| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 219,654 | A→G | G114G (GGT→GGC) | rcsF ← | outer membrane lipoprotein RcsF |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 219,654 | 0 | A | G | 95.2% | 132.8 / ‑4.3 | 42 | G114G (GGT→GGC) | rcsF | outer membrane lipoprotein RcsF |
| Reads supporting (aligned to +/- strand): ref base A (2/0); new base G (21/19); total (23/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.92e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GGCTGGCGCGGAACGGCGAACTTTTCTTTATAGGGCGAGCGAATAACGCCTATTTGCTCGAACTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTGGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCTTACGTGCGGTTGGAATGCTCGGCGGAGAGTCCTGATTAGAGGCCTGGCAAGAGTCGCCACTG > NC_000913/219518‑219789 | ggctggcGCGGAACGGCGAACTTTTCTTTATAGGGCGAGCGAATAACGCCTATTTGCTCGAACTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTGGCGTGCCg < 2:452830/140‑1 (MQ=255) aaCGGCGAACTTTTCTTTATAGGGCGAGCGAATAACGCCTATTTGCTCGAACTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTGGCGTGCCGCTGGTGACTTc > 2:54990/1‑140 (MQ=255) gCGAACTTTTCTTTATAGGGCGAGCGAATAACGCCTATTTGCTCGAACTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTGGCGTGCCGCTGGTGACTTCGCAg < 1:673844/140‑1 (MQ=255) gCGAACTTTTCTTTATAGGGCGAGCGAATAACGCCTATTTGCTCGAACTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTGGCGTGCCGCTGGTGACTTCGCAg < 1:60833/140‑1 (MQ=255) aaCTTTTCTTTATAGGGCGAGCGAATAACGCCTATTTGCTCGAACTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTGGCGTGCCGCTGGTGACTTCGCAGCTa < 2:813205/140‑1 (MQ=255) aaCTTTTCTTTATAGGGAGAGCCAATAACGCTTATTTGCACGAACTGGTAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTGGCGTGCCGCTGGTGACTTCGCAGCTa < 2:298645/140‑1 (MQ=255) cTTTTCTTTATAGGGCGAGCGAATAACGCCTATTTGCTCGAACTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTGGCGTGCCGCTGGTGACTTCGCAGCTATg < 1:54990/140‑1 (MQ=255) agtCTTTATAGGGCGTGCGAATGACTCCTCTTTGCTCGAACTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTGGCGTGCCGCTGGTGACTTCGCAGCTATGCa < 1:265522/138‑1 (MQ=255) tataGGGCGAGCGAATAACGCCTATTTGCTCGAACTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGAGGATAGCAGGCTGACTTACCGCTGGTAACTTGGCAGCTATGCAGTAATa > 2:373910/1‑140 (MQ=255) tataGGGCGAGCGAATAACGCCTATTTGCTCGAACTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTGGCGTGCCGCTGGTGACTTCGCAGCTATGCAGTAATa < 1:693199/140‑1 (MQ=255) ggCGAGCGAATAACGCCTATTTGCTCGAACTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTGGCGTGCCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCa > 1:194633/1‑140 (MQ=255) cgagcgaATAACGCCTATTTGCTCGAACTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTGGCGTGCCGCTGGTAACTACGTAGCTATGCAGTAATACAGCAtt > 1:273847/1‑140 (MQ=255) cgagcgaATAACGCCTATTTGCTCGAACTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTGGCGCGCCACTGTTGACTTCGGAGCTTTGCAGTAATACAGCact > 2:265522/1‑138 (MQ=255) gagcgaATAACGCCTATTTGCTCGAACTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTGGCGTGCCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTg > 2:650117/1‑140 (MQ=255) agcgaATAACGCCTATTTGCTCGAACTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTGGCGTGCCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTgg > 2:671503/1‑140 (MQ=255) aaTAACGCCTATTTGCTCGAACTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTGGCGTGCCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCttt < 2:194633/140‑1 (MQ=255) tttGCTCGAACTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTGGCGTGCCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCttt > 1:545725/1‑129 (MQ=255) tttGCTCGAACTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTGGCGTGCCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCttt < 2:545725/129‑1 (MQ=255) tttGCTCGAACTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTGGCGTGCCGCTGATGACTTCGGAGCAATGCAGTAATACGGCATCGGCTTTCATTTTAGAgg > 2:305314/1‑140 (MQ=255) ttGCTCGAACTGGAATCTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTGGCGTGCCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGc < 1:305314/140‑1 (MQ=255) tGCTCGAACTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTGGCGTGCCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCg < 1:650117/140‑1 (MQ=255) cGAACTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTGGCGTGCCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTag < 1:531948/131‑1 (MQ=255) cGAACTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTGGCGTGCCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTag > 2:531948/1‑131 (MQ=255) aCTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTGGCGTGCCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGAttt > 2:167544/1‑140 (MQ=255) cATTTCGCCGTAATGTTTAGCGCAGAACCGACACAAACAACCTGACGATAGCAGCCTGGCGTGCCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCTTACg < 2:282108/140‑1 (MQ=255) cATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTGGCGTGCCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCTTACg < 1:167544/140‑1 (MQ=255) cGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTGGCGTGCCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCTTACGTGCGGTTg > 1:490098/1‑140 (MQ=255) cGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTGGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCAGTGGCTTTCGTTTTAGAAGCGTTGATGTGCAACCGCTTACGTGCGGTTg > 2:262223/1‑140 (MQ=255) cgcAGAACCGATACATACAGCCTGACGATAGCAGCCTGGCGTGCCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCTTACGTGCGGTTGGAATGCTcggcg > 2:527468/1‑140 (MQ=255) cgcAGAACCGATACATACAGCCTGACGATAGCAGCCTGGCGTGCCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCTTACGTGCGGTTGGAATGCTcggcg > 1:42105/1‑140 (MQ=255) cgcAGAACCGATACATACAGCCTGACGATAGCAGCCTGGCGTGCCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCTTACGTGCGGTTGGAATGCTcggcg < 1:671503/140‑1 (MQ=255) cAGAACCGATACATAAAGCCTGACGCTAGCAGCCTGGCGTGCCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCTTACGTGCGGTTGGAATGCTCGGCGga < 1:373910/140‑1 (MQ=255) aaCCGATACATACAGCCTGACGATAGCAGCCTGGCGTGCCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATGTGCGTCCGCTTACGTGAGGTTTGAATGCTCGGCGGagag > 2:242116/1‑140 (MQ=255) atacatacAGCCTGACGATAGCAGCCTGGCGTGCCGCTGGCGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCTTACGTGCGGTTGGAATGCTCGGCGGAGAGTCCTg > 2:734495/1‑140 (MQ=255) ccTGACGATAGCAGCCTGGCGTGCCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCTTACGTGCGGTTGGAATGCTCGGCGGAGAGTCCTGATTAGAGGcc > 1:703811/1‑140 (MQ=255) gACGATAGCAGCCTGGCGTGCCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCTTACGTGCGGTTGGAATGCTCGGCGGAGAGTCCTGATTAGAGGCCTgg > 1:821075/1‑140 (MQ=255) aCGATAGCAGCCTGGCGTGCCGCTGGTGACTTCGCAGCTATGCCGTAATACAGCATTGGCTTTCCTTTTAGAGGCGTTGATTTGCATCCGCGTACGTGCGGTTGCATTTCTCGGCGGAGCGTGATGATTAGCGGCCTTGc > 2:399071/1‑140 (MQ=255) aCGATAGCAGCCTGGCGTGCCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCTTACGTGCGGTTGGAATGCTCGGCGGAGAGTCCTGATTAGAGGc < 2:308394/135‑1 (MQ=255) aCGATAGCAGCCTGGCGTGCCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCTTACGTGCGGTTGGAATGCTCGGCGGAGAGTCCTGATTAGAGGc > 1:308394/1‑135 (MQ=255) aCGATAGCAGCCTGGCGTGCCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCCTACGTGCGTTTGGAATGCTCGGCGGGGAGTGCTGATTAGAGGCCTGGc > 2:323942/1‑140 (MQ=255) cGATAGCAGCCTGGCGTGCCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCTTACGTGCGGTTGGAATGCTCGGCGGAGAGTCCTGATTAGAGGCCTGGCa < 2:490098/140‑1 (MQ=255) gCGTGCCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCTTACGTGCGGTTGGAATGCTCGGCGGAGAGTCCTGATTAGAGGCCTGGCAAGAGTCGCCACTg > 2:699138/1‑140 (MQ=255) | GGCTGGCGCGGAACGGCGAACTTTTCTTTATAGGGCGAGCGAATAACGCCTATTTGCTCGAACTGGAAACTGCTCATTTCGCCGTAATGTTAAGCGCAGAACCGATACATACAGCCTGACGATAGCAGCCTGGCGTACCGCTGGTGACTTCGCAGCTATGCAGTAATACAGCATTGGCTTTCATTTTAGAGGCGTTGATTTGCATCCGCTTACGTGCGGTTGGAATGCTCGGCGGAGAGTCCTGATTAGAGGCCTGGCAAGAGTCGCCACTG > NC_000913/219518‑219789 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |