Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,784,065	G→A	V363V (GTG→GTA)	fadK →	short chain acyl‑CoA synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,784,065	0	G	A	100.0%	137.0 / NA	40	V363V (GTG→GTA)	fadK	short chain acyl‑CoA synthetase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (22/18);  total (22/18)

CGGACCTTTCAGCGCTGCGTTTCTTTCTTTGCGGCGGAACCACAATCCCCAAAAAAGTGGCGCGTGAATGCCAGCAGCGCGGCATTAAATTATTAAGTGTTTATGGTTCCACAGAAAGTTCGCCGCATGCGGTGGTGAATCTCGATGATCCTTTGTCGCGCTTTATGCACACCGATGGTTACGCTGCCGCAGGTGTAGAGATTAAAGTGGTCGATGACGCACGCAAGACCTTACCGCCAGGTTGCGAAGGTGAAGAAGCCTCGCGTGGCCCC  >  NC_000913/1783929‑1784200                                                                                                                                         |                                                                                                                                        cgGACCTTTCAGCGCTGCGTTTCTTTCTTTGCGGCGGAACCACAATCCCCAAAAAAGTGGCGCGTGAATGCCAGCAGCGCGGCATTAAATTGTTAAGTGTTTATAGATGAACAGAAACTTCGGACCATGCGGGGGTAAAt                                                                                                                                      >  2:422472/1‑140 (MQ=255) cgGACCTTTCAGCGCTGCGTTTCTTTCTTTGCGGCGGAACCACAATCCCCAAAAAAGTGGCGCGTGAATGCCAGCAGCGCGGCATTAAATTATTAAGTGTTTATGGTTCCACAGCAAATTCGCCACATGCGGTGGTAAAt                                                                                                                                      >  1:421241/1‑140 (MQ=255) cgGACCTTTCAGCGCTGCGTTTCTTTCTTTGCGGCGGAACCACAATCCCCAAAAAAGTGGCGCGTGAATGCCAGCAGCGCGGCATTAAATTATTAAGTGTTTATGGTTCCACAGAAAGTTCGCCGCATGCGGTGGTAAAt                                                                                                                                      >  1:604267/1‑140 (MQ=255)  ggACCTTTCAGCGCTGCGTTTCTTTCTTTGCGGCGGAACCACAATCCCCAAAAAAGTGGCGCGTGAATGCCAGCAGCGCGGCATTAAATTATTAAGTGTTTATGGTTCCACAGAAAGTTCGCCGCATGCGGTGGTAAAtc                                                                                                                                     >  1:180909/1‑140 (MQ=255)    aCCTTTCAGCGCTGCGTTTCTTTCTTTGCGGCGGAACCACAATCCCCAAAAAAGTGGCGCGTGAATGCCAGCAGCGCGGCATTAAATTATTAAGTGTTTATGGTTCCACAGAAAGTTCGCCGCATGCGGTGGTAAAtctc                                                                                                                                   >  1:577425/1‑140 (MQ=255)      cTTTCAGCGCTGCGTCTCTTTCCTTACGGTGGAACCACAATCCCCAAAAAAGTGGCGCGTGAATGCCAGCAGCGCGGCATTAAATTATTAAGTGTTTATGGTTCCACAGAAAGTTCGCCGCATGCGGTGGTAAATCTCga                                                                                                                                 <  1:321113/140‑1 (MQ=255)        ttCAGCGCTGCGTTTCTTTCTTTGCGGCGGAACCACAATCCCCAAAAAAGTGGCGCGTGAATGCCAGCAGCGCGGCATTAAATTATTAAGTATTTATGGTTCCAGAGAAAGTTCGCCGCATGTGGTGGTAAATCTCgatg                                                                                                                               >  2:242212/1‑140 (MQ=255)                  cGTTTCTTTCTTTGCGGCGGAACCACAATCCCCAAAAAAGTGGCGCGTGAATGCCAGCAGCGCGGCATTAAATTATTAAGTGTTTATGGTTCCACAGAAAGTTCGCCGCATGCGGTGGTAAATCTCGATGATCCTTTGTc                                                                                                                     >  1:138280/1‑140 (MQ=255)                  cGTTTCTTTCTTTGCGCCGGAATCACAATCCCCTATAAATTGGCGCGTCAATGCCAGCAGCGCGGCATTAAATTATTAAGTGTTTATGGTTCCACAGAAAGTTCGCCGCATGCGGTGGTAAATCTCGATGATCCTTTGTc                                                                                                                     <  2:421241/140‑1 (MQ=255)                  cGTTTCTTTCTTTGCCGCGGAACCACAATTCCCAAAACAGTGGCGCGTGAATGCCAGCAGCGCGGCATTAAATTATTAAGTGTTTATGGTTCCACAGAAAGTTCGCCGCATGCGGTGGTAAATCTCGATGATCCTTTGTc                                                                                                                     <  1:393980/140‑1 (MQ=255)                      tctttcttTGCGGCGTAACCACAATGCCCAAAAAAGTCGCGCGTGAATGCCAGCAGCGCGGCATTAAATTATTAAGTGTTTATGGTTCCACAGAAAGTTCGCCGCATGCGGTGGTAAATCTCGATGATCCTTTGTCgcgc                                                                                                                 <  1:330467/140‑1 (MQ=255)                                cggcggAACCACAATCCCCAAAAAAGTGGCGCGTGAATGCCAGCAGCGCGGCATTAAATTATTAAGTGTTTATGGTTCCACAGAAAGTTCGCCGCATGCGGTGGTAAATCTCGATGATCCTTTGTCGCGCTTTATGcaca                                                                                                       <  2:448774/140‑1 (MQ=255)                                          acaATCCCCAAAAAAGTGGCGCGTGAATGCCAGCAGCGCGGCATTAAATTATTAAGTGTTTATGGTTCCACAGAAAGTTCGCCGCATGCGGTGGTAAATCTCGATGATCCTTTGTCGCGCTTTATGCACACCGATGGTTa                                                                                             <  1:132160/140‑1 (MQ=255)                                                cccAAAAAAGTGGCGCGTGAATGCCAGCAGCGCGGCATTAAATTATTAAGTGTTTATGGTTCCACAGAAAGTTCGCCGCATGCGGTGGTAAATCTCGATGATCCTTTGTCGCGCTTTATGCACACCGATGGTTAc                                                                                            <  2:737764/135‑1 (MQ=255)                                                cccAAAAAAGTGGCGCGTGAATGCCAGCAGCGCGGCATTAAATTATTAAGTGTTTATGGTTCCACAGAAAGTTCGCCGCATGCGGTGGTAAATCTCGATGATCCTTTGTCGCGCTTTATGCACACCGATGGTTAc                                                                                            >  1:737764/1‑135 (MQ=255)                                                cccAAAAAAGTGGCGCGTGAATGCCAGCAGCGCGGCATTAAATTATTAAGTGTTTATGGTTCCACAGAAAGTTCGCCGCATGCGGTGGTAAATCTCGATGATCCTTTGTCGCGCTTTATGCACACCGATGGTTACGCTGc                                                                                       >  1:771021/1‑140 (MQ=255)                                                  cAAAAAAGTGGCGCGTGAATGCCAGCAGCGCGGCATTAAATTATTAAGTGTTTATGGTTCCACAGAAAGTTCGCCGCATGCGGTGGTAAATCTCGATGATCCTTTGTCGCGCTTTATGCACACCGATGGTTACGCTGCCg                                                                                     <  2:771021/140‑1 (MQ=255)                                                   aaaaaaGTGGCGCGTTAATGCCCGCAGCGGGGCATTACATTATTAAGTGTTTATGGTTCCACAGAAAGTTCGCCGCATGCGGTGGTAAATCTCGATGATCCTTTGTCGCGCTTTATGCACACCGATGGTTACGCTGCCGc                                                                                    <  1:422472/140‑1 (MQ=255)                                                   aaaaaaGTGGCGCGTGAATGCCAGCAGCGCGGCATTAAATTATTAAGTGTTTATGGTTCCACAGAAAGTTCGCCGCATGCGGTGGTAAATCTCGATGATCCTTTGTCGCGCTTTATGCACACCGATGGTTACGCTGCCGc                                                                                    >  2:728544/1‑140 (MQ=255)                                                          tGGCGCGTGAATGCCTGCAGCGTGGTATTAAATTATTGAGTGTTTATGGTTCCACAGAAAGTTCGCCGCATGCGGTGGTAAATCTCGATGATCCTTTGTCGCGCTTTATGCACACCGATGGTTACGCTGCCGCAGGTGTa                                                                             <  1:333501/140‑1 (MQ=255)                                                          tGGCGCGTGAATGCCAGCAGCGCGGCATTAAATTATTAAGTGTTTATGGTTCCACAGAAAGTTCGCCGCATGCGGTGGTAAATCTCGATGATCCTTTGTCGCGCTTTATGCACACCGATGGTTACGCTGCCGCAGGTGTa                                                                             <  1:728544/140‑1 (MQ=255)                                                                              cgcgGCATTAAATTATTAAGTGTTTATGGTTCCACAGAAAGTTCGCCGCATGCGGTGGTAAATCTCGATGATCCTTTGTCGCGCTTTATGCACACCGATGGTTACGCTGCCGCAGGTGTAGAGATTAAAGTGGTCGATGa                                                         >  1:719979/1‑140 (MQ=255)                                                                              cgcgGCATTAAATTATTAAGTGTTTATGGTTCCACAGAAAGTTCGCCGCATGCGGTGGTAAATCTCGATGATCCTTTGTCGCGCTTTATGCACACCGATGGTTACGCTGCCGCAGGTGTAGAGATTAAAGTGGTCGATGa                                                         >  2:100425/1‑140 (MQ=255)                                                                                cgGCATTAAATTATTCAGTGTTCATCGTTCCACAGAAAGTTCGCCGCATGCGGTGGTAAATCTCGATGATCCTTTGTCGCGCTTTATGCACACCGATGGTTACGCTGCCGCAGGTGTAGAGATTAAAGTGGTCGATGacg                                                       <  1:267736/140‑1 (MQ=255)                                                                                cgGCATTAAATTATTAAGTGTTTATGGTTCCACAGAAAGTTCGCCGCATGCGGTGGTAAATCTCGATGATCCTTTGTCGCGCTTTATGCACACCGATGGTTACGCTGCCGCAGGTGTAGAGATTAAAGTGGTCGATGacg                                                       <  1:100425/140‑1 (MQ=255)                                                                                cgGCATTAAATTATTAAGTGTTTATGGTTCCACAGAAAGTTCGCCGCATGCGGTGGTAAATCTCGATGATCCTTTGTCGCGCTTTATGCACACCGATGGTTACGCTGCCGCAGGTGTAGAGATTAAAGTGGTCGATGacg                                                       <  2:466488/140‑1 (MQ=255)                                                                                  gCATTAAATTAGTAAGTGTTTATGGTTCCACAGAAAGTTCGCCGCATGCGGTGGTAAATCTCGATGATCCTTTGTCGCGCTTTATGCACACCCATGGTTTCGCTACCGCAGGTGTGGAGATTAAAGTGGTCGATGacgca                                                     <  2:79985/140‑1 (MQ=255)                                                                                             ttaAGTGTTTATGGTTCCACAGAAAGTTCGCCGCATGCGGTGGTAAATCTCGATGATCCTTTGTCGCGCTTTATGCACACCGATGGTTACGCTGCCGCAGGTGTAGAGATTAAAGTGGTCGATGACGCACGCAAGACCtt                                          >  2:515092/1‑140 (MQ=255)                                                                                             ttaAGTGTTTATGGTTCCACAGAAAGATCGCCGCATGCGGTGGTAAATCTCGATGATCCTTTGTCGCGCTTTATGCACACCGATGGTTACGCTGCCGCAGGTGTAGAGATTAAAGTGGTCGATGACGCACGCAAGACCtt                                          >  2:97160/1‑140 (MQ=255)                                                                                                  tgtTTATGGTTCCACAGAAAGTTCGCCGCATGCGGTGGTAAATCTCGATGATCCTTTGTCGCGCTTTATGCACACCGATGGTTACGCTGCCGCAGGTGTAGAGATTAAAGTGGTCGATGACGCACGCAAGACCTTACCGc                                     >  1:654775/1‑140 (MQ=255)                                                                                                  tgtTTATGGTTCCACAGAAAGTTCGCCGCATGCGGTGGTAAATCTCGATGATCCTTTGTCGCGCTTTATGCACACCGATGGTTACGCTGCCGCAGGTGTAGAGATTAAAGTGGTCGATGACGCACGCAAGACCTTACCGc                                     >  2:577355/1‑140 (MQ=255)                                                                                                  tgtTTATGGTTCCACAGAAAGTTCGCCGCATGCGGTGGTAAATCTCGATGATCCTTTGTCGCGCTTTATGCACACCGATGGTTACGCTGCCACAGGTGTAAAGCATAATGTGGTCGATGACGGCCGCAAGACCTTACCGc                                     >  2:290562/1‑140 (MQ=255)                                                                                                    tttATGGTTCCACAGAAAGTTCGCCGCATGCGGTGGTAAATCTCGATGATCCTTTGTCGCGCTTTATGCACACCGATGGTTACGCTGCCGCAGGTGTAGAGATTAAAGTGGTCGATGACGCACGCAAGACCTTACCGCCa                                   >  1:499886/1‑140 (MQ=255)                                                                                                      tATGGTTCCACAGAAAGTTCGCCGCATGCGGTGGTAAATCTCGATGATCCTTTGTCGCGCTTTATGCACACCGATGGTTACGCTGCCGCAGGTGTAGAGATTAAAGTGGTCGATGACGCACGCAAGACCTTACCGCCAgg                                 >  2:59050/1‑140 (MQ=255)                                                                                                      tATGGTTCCACAGAAAGTTCGCCGCATGCGGTGGTAAATCTCGATGATCCTTTGTCGCGCTTTATGCACACCGATGGTTACGCTGCCGCAGCTGCAGAGATTAACGTGTTCGATGACTCACGCAAGACCTTACCGCCggg                                 >  2:323692/1‑140 (MQ=255)                                                                                                       aTGGTTCCACAGAAAGTTCGCCGCATGCGGTGGTAAATCTCGATGATCCTTTGTCGCGCTTTATGCACACCGATGGTTACGCTGCCGCAGGTGTAGAGATTAAAGTGGTCGATGACGCACGCAAGACCTTACCGCCAGGt                                >  2:45718/1‑140 (MQ=255)                                                                                                          gTTCCACAGAAAGTTCGCCGCATGCGGTGGTAAATCTCGATGATCCTTTGTCGCGCTTTATGCACACCGATGGTTACGCTGCCGCAGGTGTAGAGATTAAAGTGGTCGATGACGCACGCAAGACCTTACCGCCAGGTTGc                             <  2:180909/140‑1 (MQ=255)                                                                                                            tCCACAGAAAGTTCGCCGCATGCGGTGGTAAATCTCGATGATCCTTTGTCGCGCTTTATGCACACCGATGGTTACGCTGCCGCAGGTGTAGAGATTAAAGTGGTCGATGACGCACGCAAGACCTTACCGCCAGGTTGCGa                           >  1:857754/1‑140 (MQ=255)                                                                                                              cacaGAAAGTTCGCCGCATGCGGTGGTAAATCTCGATGATCCTTTGTCGCGCTTTATGCACACCGATGGTTACGCTGCCGCAGGTGTAGAGATTAAAGTGTTCGGTGAGGCACGCAATACCTTACCGCCAGGTTGCGAAg                         >  2:231879/1‑140 (MQ=255)                                                                                                                        tCGCCGCATGCGGTGGTAAATCTCGATGATCCTTTGTCGCGCTTTATGCACACCGATGGTTACGCTGCCGCAGGTGTAGAGATTAAAGTGGTCGATGACGCACGCAAGACCTTACCGCCAGGTTGCGAAGGTGAAGAAGc               <  1:577355/140‑1 (MQ=255)                                                                                                                              cATGCGGTGGTAAATCTCGATGATCCTTTGTCGCGCTTTATGCACACCGATGGTTACGCTGCCGCAGGTGTAGAGATTAAAGTGGTCGATGACGCACGCAAGACCTTACCGCCAGGTTGCGAAGGTGAAGAAGCCTcgcg         <  1:515092/140‑1 (MQ=255)                                                                                                                                tGCGGTGGTAAATCTCGATGATCCTATGTAGCGCTTTATGCACACCGATGGTTACGCTGCCGCAGGTGTAGAGATTAAAGGGGTCGATGACGCACGCAAGACCTTACCGCCAGGTTGCGAAGGTGAAGAAGCCTCGCGTg       <  1:290562/140‑1 (MQ=255)                                                                                                                                     tggtaAATCTCGATGATCCTTTGTCGCGCTTTATGCACACCGATGGTTACGCTGCCGCAGGTGTAGAGATTAAAGTGGTCGATGACGCACGCAAGACCTTACCGCCAGGTTGCGAAGGTGAAGAAGCCTCGCGTGGcccc  <  1:80772/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                        CGGACCTTTCAGCGCTGCGTTTCTTTCTTTGCGGCGGAACCACAATCCCCAAAAAAGTGGCGCGTGAATGCCAGCAGCGCGGCATTAAATTATTAAGTGTTTATGGTTCCACAGAAAGTTCGCCGCATGCGGTGGTGAATCTCGATGATCCTTTGTCGCGCTTTATGCACACCGATGGTTACGCTGCCGCAGGTGTAGAGATTAAAGTGGTCGATGACGCACGCAAGACCTTACCGCCAGGTTGCGAAGGTGAAGAAGCCTCGCGTGGCCCC  >  NC_000913/1783929‑1784200

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑