Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,799,072	T→C	intergenic (‑130/+154)	pheS ← / ← pheM	phenylalanine‑‑tRNA ligase subunit alpha/pheST‑ihfA operon leader peptide

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,799,072	0	T	C	100.0%	97.9 / NA	29	intergenic (‑130/+154)	pheS/pheM	phenylalanine‑‑tRNA ligase subunit alpha/pheST‑ihfA operon leader peptide
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (16/13);  total (16/13)

TGTGACATGGTTTTCCTCATTGTGTCAGTGGTGACACTGGTTCGTTGGACTTAGAGCCTATCCCATCAGGCTATTTTACTTGCCATTTTGGTCCCGGGCTGTGCTCGAAATTCTCACGTACTTAAATACGCTCCGGTTTCTCCGCGCTGGCCGTGTCCAGTCTGGCTGCGACAATTACACCTGATGAGACAGGCTTTTTATTTTTCAAAACGCGCATACAAAAAAAGCCTCCACTGGGAGGCTTTCAGGCGCTGTTTTCCGTTTCTCTTCTCA  >  NC_000913/1798935‑1799207                                                                                                                                          |                                                                                                                                        tgtgACATGGTTTTCCTCATTGTGTCAGTGGTGACACTGGTTCGTTGGACTTAGAGCCTATCCCATCAGGCTATTTTACTTGCCATTTTGGTCCCGGGCTGTGCTCGAAATTCTCACGTACTTAAATACGCTCCGGtctc                                                                                                                                       >  1:711910/1‑140 (MQ=255)         ggTTTTCCTCATTGTGTCAGTGGTGACACTGGTTCGTTGGACTTAGAGCCTATCCCATCAGGCTATTTTACTTGCCATTTTGGTCCCGGGCTGTGCTCGAAATTCTCACGTACTTAAATACGCTCCGGTCTCTCCGCGCt                                                                                                                               >  2:121507/1‑140 (MQ=255)           ttttCCTCATTGTGTCAGTGGTGACACTGGTTCGTTGGACTTAGAGCCTATCCCATCAGGCTATTTTACTTGCCATTTTGGTCCCGGGCTGTGCTCGAAATTCTCACGTACTTAAATACGCTCCGGTCTCTCCGCGCTgg                                                                                                                             <  2:752796/140‑1 (MQ=255)           ttttCCTCATTGTGTCAGTGGTGACACTGGTTCGTTGGACTTAGAGCCTATCCCATCAGGCTATTTTACTTGCCATTTTGGTCCAGGGCTGTGCTCGAAATTCTCACGTACTTAAATACGCTCCGGTCTCTCCGCGCTgg                                                                                                                             >  1:650874/1‑140 (MQ=255)            tttCCTCATTGTGTCAACGGTGACACTGGTTCGCTGGACGTAGAGCCTATCCCATCAGGCTATTTTACTTGCCATTTTGGTCCCGGGCTGTGCTCGAAATTCTCACGTACTTAAATACGCTCCGGTCTCTCCGCGCTGGc                                                                                                                            <  2:389154/140‑1 (MQ=255)            agtCCTCATTGTGTCAGTGGTGACATTGGGTCGTTGGGCTTAGAGCCTATCCCATCAGGCTATTTTACTTGCCATTTTGGTCCCGGGCTGTGCTCGAAATTCTCACGTACTTAAATACGCTCCGGTCTCTCCGCGCTGGc                                                                                                                            <  1:380600/138‑1 (MQ=255)                cTCATTGTGTCAGTGGTGACACTGGTTCGTTGGACTTAGAGCCTATCCCATCAGGCTATTTTACTTGCCATTTTGGTCCCGGGCTGTGCTCGAAATTCTCACGTACTTAAATACGCTCCGGTCTCTCCGCGCTGGCCgtg                                                                                                                        <  1:121507/140‑1 (MQ=255)                cTCATTGTGTCAGTGGTGACACTGGTTCGTTGGACTTAGAGCCTATCCCATCAGGCTATTTTACTTGCCATTTTGGTCCAGGGCTGTGCTCGAAATTCTCACGTACTTAAATACGCTCCGGTCTCTCCGCGCTGGCCgtg                                                                                                                        <  2:650874/140‑1 (MQ=255)                       tgtCAGTGGTGACACTGGTTCGTTGGACTTAGAGCCTATCCCATCAGGCTATTTTACTTGCCATTTTGGTCCCGGGCTGTGCTCGAAATTCTCACGTACTTAAATACGCTCCGGTCTTTCCGCGATGGCCGTGTCCAGTc                                                                                                                 >  1:389154/1‑140 (MQ=255)                         tCAGTGGTGACACTGGTTCGTTGGACTTAGAGCCTATCCCATCAGGCTATTTTACTTGCCATTTTGGTCCCGGGCTGTGCTCGAAATTCTCACGTACTTAAATAGGCTCCGGTCTCTACGCGCAGGCCGTGTCCAGTcct                                                                                                               >  2:380600/1‑138 (MQ=255)                                 gACACTGGTTCGTTGGACTTAGAGCCTATCCCATCAGGCTATTTTACTTGCCATTTTGGTCCCGGGCTGTGCTCGAAATTCTCACGTACTTAAATACGCTCCGGTCTCTCCGCGCTGGCCGTGTCCAGTCTGGCTGCGAc                                                                                                       >  1:105931/1‑140 (MQ=255)                                    acTGGTTCGTTGGACTTAGAGCCTATCCCATCAGGCTATTTTACTTGCCATTTTGGTCCCGGGCTGTGCTCGAAATTCTCACGTACTTAAATACGCTCCGGTCTCTCCGCGCTGGCCGTGTCCAGTCTGGCTGCGACAAt                                                                                                    <  2:711910/140‑1 (MQ=255)                                       ggTTCGTTGGACTTAGAGCCTATCCCATCAGGCTATTTTACTTGCCATTTTGGTCCCGGGCTGTGCTCGAAATTCTCACGTACTTAAATACGCTCCGGTCTCTCCGCGCTGGCCGTGTCCAGTCTGGCTGCGACAATTac                                                                                                 >  1:744837/1‑140 (MQ=255)                                         ttCGTTGGACTTAGAGCCTATCCCATCAGGCTATTTTACTTGCCATTTTGGTCCCGGGCTGTGCTCGAAATTCTCACGTACTTAAATACGCTCCGGTCTCTCCGCGCTGGCCGTGTCCAGTCTGGCTGCGACAATTacac                                                                                               >  1:482371/1‑140 (MQ=255)                                                  cTTAGAGCCTATCCCATGAGGCCATTTTACTCGCCTTTTCGGTCCCGGGCTGTGCTCGAAATTCTCACGTACTTAAATACGCTCCGGTCTCTCCGCGCTGGCCGTGTCCAGTCTGGCTGCGACAATTACACCTGATgaga                                                                                      <  2:327275/140‑1 (MQ=255)                                                  cTTAGAGCCTATCCCATCAGGCTATTTTACTTGCCATTTTGGTCCCGGGCTGTGCTCGAAATTCTCACGTACTTAAATACGCTCCGGTCTCTCCGCGCTGGCCGTGTCTAGTCTGGCTGCGACAATTACACCTGATgaga                                                                                      >  2:410003/1‑140 (MQ=255)                                                                       cTATTTTACTTGCCATTTTGGTCCCGGGCTGTGCTCGAAATTCTCACGTACTTAAATACGCTCCGGTCTCTCCGCGCTGGCCGTGTCCAGTCTGGCTGCGACAATTACACCTGATGAGACAGGCTTTTTATTTTTCaaaa                                                                 >  2:88146/1‑140 (MQ=255)                                                                                         tGGTCCCGGGCTGTGCTCGAAATTCTCACGTACTTAAATACGCTCCGGTCTCTCCGCGCTGGCCGTGTCCAGTCTGGCTGCGACAATTACACCTGATGAGACAGGCTTTTTATTTTTCAAAACGCGCATACAAAAAAAGc                                               <  1:88146/140‑1 (MQ=255)                                                                                         tGGTCCCGGGCTGTGCTCGAAATTCTCACGTACTTAAATACGCTCCGGTCTCTCCGCGCTGGCCGTGTCCAGTCTGGCTGCGACAATTACACCTGATGAGACAGGCTTTTTATTTTTCAAAACGCGCATACAAAAAAAGc                                               <  2:744837/140‑1 (MQ=255)                                                                                                               ttCTCACGTACTTAACTACGCTGCGATCTATCCGCGCGGGCCGTGTCCAGTCTGGCTGCGACAATTACACCTGATGAGACAGGCTTTTTATTTTTCAAAACGCGCATACAAAAAAAGCCTCCACTGGGAGGCTTTCAGgc                         <  1:410003/140‑1 (MQ=255)                                                                                                               ttCTCACGTACTTAAATACGCTCCGGTCTCTCCGCGCTGGCCGTGTCCAGTCTGGCTGCGACAATTACACCTGATGAGACAGGCTTTTTATTTTTCAAAACGCGCATACAAAAAAAGCCTCCACTGGGAGGCTTTCAGgc                         <  2:105931/140‑1 (MQ=255)                                                                                                                tctcACGTACTTAAATACGCTCCGGTCTCTCCGCGCTGGCCGTGTCCAGTCTGGCTGCGACAATTACACCTGATGAGACAGGCTTTTTATTTTTCAAAACGCGCATACAAAAAAAGCCTCCACTGGGAGGCTTTCAGgcg                        >  1:689817/1‑140 (MQ=255)                                                                                                                  tcACGTACTTAAATACGCTCCGGTCTCTCCGCGCTGGCCGTGTCCAGTCTGGCTGCGACAATTACACCTGATGAGACAGGCTTTTTATTTTTCAAAACGCGCTTACAAAAAAAGTCTGCACTGGCAGGCTTTCAGGCGCt                      >  1:401034/1‑140 (MQ=255)                                                                                                                        aCTTAAATACGCTCCGGTCTCTCCGCGCTGGCCGTGTCCAGTCTGGCTGCGACAATTACACCTGATGAGACAGGCTTTTTATTTTTCAAAACGCGCATACAAAAAAAGCCTCCACTGGGAGGCTTTCAGGCGCTGTTTTc                <  2:482371/140‑1 (MQ=255)                                                                                                                                    tCCGGTCTCTCCGCGCTGGCCGTGTCCAGTCTGGCTGCGACAATTACACCTGATGAGACAGGCTTTTTATTTTTCAAAACGCGCATACAAACAAAGCCTCCACTTGGACGCTTTCAGCCGCTCGTTTCCGTttctcttct    >  2:360540/1‑140 (MQ=255)                                                                                                                                    tCCGGTCTCTCCGCGCTGGCCGTGTCCAGTCTGGCTGCGACAATTACACCTGATGAGACAGGCTTTTTATTTTTCAAAACGCGCATACAAAAAAAGCCTCCAGTGGGAGGGTTTGAGGCGCTGTGTTCCGTttctcttct    >  1:219268/1‑140 (MQ=255)                                                                                                                                    tCCGGTCTCTCCGCGCTGGCCGTGTCCAGTCTGGCTGCGACAATTACACCTGATGAGACAGGCTTTTTATTTTTCAAAACGCGCATACAAAAAAAGCCTCCACTGGGAGGCTTTCAGGCGCTGTTTTCCGTttctcttct    >  1:730225/1‑140 (MQ=255)                                                                                                                                    tCCGGTCTCTCCGCGCTGGCCGTGTCCAGTCTGGCTGCGACAATTACACCTGATGAGACAGGCTTTTTATTTTTCAAAACGCGCATACAAAAAAAGCCTCCACTGGGAGGCTTTCAGGCGCTGTTTTCCGTttctcttct    >  1:703202/1‑140 (MQ=255)                                                                                                                                    tCCGGTCTCTCCGCGCTGGCCGTGTCCAGTCTGGCTGCGACAATTACACCTGATGAGACAGGCTTTTTATTTTTCAAAACGCGCATACAAAAAAAGCCTCCACTGGGAGGCTTTCAGGCGCTGTTTTCCGTttctcttct    >  1:192630/1‑140 (MQ=255)                                                                                                                                     ccGGTCTCTCCGCGCTGGCCGTGTCCAGTCTGGCTGCGACAATTACACCTGATGAGACAGGCTTTTTATTTTTCAAAACGCGCATACAAAAAAAGCCTCCACTGGGAGGCTTTCAGGCGCTGTTTTCCGTttctcttctc   <  1:83837/140‑1 (MQ=255)                                                                                                                                      cGGTCTCTCCGCGCTGGCCGTGTCCAGTCTGGCTGCGACAATTACACCTGATGAGACAGGCTTTTTATTTTTCAAAACGCGCATACAAAAAAAGCCTCCACTGGGAGGCTTTCAGGCGCTGTTTTCCGTTTCTCTTCTCa  <  2:703202/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                        TGTGACATGGTTTTCCTCATTGTGTCAGTGGTGACACTGGTTCGTTGGACTTAGAGCCTATCCCATCAGGCTATTTTACTTGCCATTTTGGTCCCGGGCTGTGCTCGAAATTCTCACGTACTTAAATACGCTCCGGTTTCTCCGCGCTGGCCGTGTCCAGTCTGGCTGCGACAATTACACCTGATGAGACAGGCTTTTTATTTTTCAAAACGCGCATACAAAAAAAGCCTCCACTGGGAGGCTTTCAGGCGCTGTTTTCCGTTTCTCTTCTCA  >  NC_000913/1798935‑1799207

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑