| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,870,094 | T→C | R380R (CGT→CGC) | yeaH → | DUF444 domain‑containing protein YeaH |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,870,094 | 0 | T | C | 98.3% | 199.3 / ‑4.6 | 58 | R380R (CGT→CGC) | yeaH | DUF444 domain‑containing protein YeaH |
| Reads supporting (aligned to +/- strand): ref base T (1/0); new base C (31/26); total (32/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01 | |||||||||||
CACAGTGGAATATTTACGCTGCACAAGCATCGGACGGCGATAACTGGGCCGATGACTCTCCGCTTTGCCATGAAATCCTGGCGAAAAAATTATTACCTGTTGTTCGTTATTACAGCTATATCGAAATTACCCGTCGTGCACATCAGACATTGTGGCGAGAATATGAGCATCTGCAATCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTA > NC_000913/1869958‑1870230 | cacaGTGGAATATTTACGCTGCACAAGCATCGGACGGCGATAACTGGGCCGATGACTCTCCGCTTTGCCATGAAATCCTGGCGAAAAAATTATTACCTGTTGTTCGTTATTACAGCTATATCGAAATTACCCGTCGCGca > 1:835294/1‑140 (MQ=255) tGGACTATTTACGCTGCACAAGCATCGGACGGCGATAACTGGGCCGATGACTCTCCGCTTTGCCATGAAATCCTGGCGAAAAAATTATTACCTGTTGTTCGTTATTACAGCTATATCGAAATTACCCGTCGCGCACATCa > 2:117391/1‑140 (MQ=255) tttACGCTGCACAAGCATCGGACGGCGATAACTGGGCCGATGACTCTCCGCTTTGCCATGAAATCCTGGCGAAAAAATTATTACCTGTTGTTCGTTATTACAGCTATATCGAAATTACCCGTCGCGCACATCAGACATtg < 1:528987/140‑1 (MQ=255) aCGCTGCACAAGCATCGGACGGCGATAACTGGGCCGATGACTCTCCGCTTTGCCATGAAATCCTGGCGAAAAAATTATTACCTGTTGTTCGTTATTACAGCTGTATCGAAATTAACCTTCGCGCACATCAGACATTGTgg > 1:309692/1‑140 (MQ=255) aCGCTGCACAAGCATCGGACGGCGATAACTGGGCCGATGACTCTCCGCTTTGCCATGAAATCCTGGCGAAAAAATTATTACCTGTTGTTCGTTATTACAGCTATATCGAAATTACCCGTCGCGCACATCAGACATTGTgg > 1:739581/1‑140 (MQ=255) aCGCTGCACAAGCATCGGACGGCGATAACTGGGCCGATGACTCTCCGCTTTGCCATGAAATCCTGGCGAAAAAATTATTACCTGTTGTTCGTTATTACAGCTATATCGAAATTACCCGTCGCGCACATCAGACATTGTgg > 2:49045/1‑140 (MQ=255) aCGCTGCACAAGCATCGGACGGCGATAACTGGGCCGATGACTCTCCGCTTTGCCATGAAATCCTGGCGAAAAAATTATTA‑CTGTTGTTCGTTATTACAGCTATATCGAAATTACCCGTCGTGCACATCAGACATTGTGGc > 2:461970/1‑140 (MQ=255) cGCTGCACAAGCATCGGCCGGCCATAACTGGACCGCTGAATCTCCGCTATGCCATGAAATCCTGGCGAAAAAATTATTACCTGTTGTTCGTTATTACAGCTATATCGAAATTACCCGTCGCGCACATCAGACATTGTGGc < 2:369114/140‑1 (MQ=255) cGCTGCACAAGCATCGGACGGCTATAACTGGCCCGCTGATTCTCCGCTATGCCATGAAATCCTGGCGAAAAAATTATTACCTGTTGTTCGTTATTACAGCTATATCGAAATTACCCGTCGCGCACATCAGACATTGTGGc < 2:272046/140‑1 (MQ=255) cGCTGCACAAGCATCGGACGGCTATAACTCGGCCGATGACTCTCCGCTTTGCCATGAAATCCTGGCGAAAAAATTATTACCTGTTGTTCGTTATTACAGCTATATCGAAATTACCCGTCGCGCACATCAGACATTGTGGc < 1:395805/140‑1 (MQ=255) cGCTGCACAAGCATCGGACGGCGATAACTGGGCCGATGACTCTCCGCTTTGCCATGAAATCCTGGCGAAAAAATTATTACCTGTTGTTCGTTATTACAGCTATATCGAAATTACCCGTCGCGCACATCAGACATTGTGGc < 1:90485/140‑1 (MQ=255) cGCTGCACAAGCATCGGACGGCGATAACTGGGCCGATGACTCTCCGCTTTGCCATGAAATCCTGGCGAAAAAATTATTACCTGTTGTTCGTTATTACAGCTATATCGAAATTACCCGTCGCGCACATCAGACATTGTGGc < 2:857028/140‑1 (MQ=255) cGCTGCACAAGCATCGGACGGCGATAACTCGGCCGATGACTCTCCGCTTTGCCATGAAATCCTGGCGAAAAAATTATTACCTGTTGTTCGTTATTACAGCTATATCGAAATTACCCGTCGCGCACATCAGACATTGTGGc < 1:388859/140‑1 (MQ=255) cTGCACAAGCATCGGACGGCGATAACTGGGCCGATGACTCTCCGCTTTGCCATGAAATCCTGGCGAAAAAATTATTACCTGTTGTTCGTTATTACAGCTATATCGAAATTACCCGTCGCGCACATCAGACATTGTGGCga < 2:741657/140‑1 (MQ=255) gCACAAGCATCGGACGGCGATATCTGGGCCGCTGAGTCTACGCTTTGCCATGAAATCCTGGCGAAAAAATTATTACCTGTTGTTCGTTATTACAGCTATATCGAAATTACCCGTCGCGCACATCAGACATTGTGGCgaga < 2:267398/140‑1 (MQ=255) cacaAGCATCGGACGGCGATAACTGGGCCGATGACTCTCCGCTTTGCCATGAAATCCTGGCGAAAAAATTATTACCTGTTGTTCGTTATTACAGCTATATCGAAATTACCCGTCGCGCACATCAGACATTGTGGCGAGaa > 2:523580/1‑140 (MQ=255) acaAGCATCGGACGGCGATAACTGGGCCGATGGCTCTCCGCTTTGCCATGAAATCCTGGCGAAAAAATTATTACCTGTTGTTCGTTATTACAGCTATATCGAAATTACCCGTCGCGCACATCAGACATTGTGGc < 2:425064/134‑1 (MQ=255) acaAGCATCGGACGGCGATAACTGGGCCGATGACTCTCCGCTTTGCCATGAAATCCTGGCGAAAAAATTATTACCTGTTGTTCGTTATTACAGCTATATCGAAATTACCCGTCGCGCACATCAGACATTGTGGc > 1:425064/1‑134 (MQ=255) acaAGCATCGGACGGCGATAACTGGGCCGATGACTCTCCGCTTTGCCATGAAATCCTGGCGAAAAAATTATTACCTGTTGTTCGTTATTACAGCTATATCGAAATTACCCGTCGCGCACATCAGACATTGTGGCGAGAat > 1:844124/1‑140 (MQ=255) cATCGGACGGCGATAACTGGGCCGATGACTCTCCGCTTTGTCATGAAATCCTGGCGAAAAAATTATTACCTGTTGTTCGTTATTACAGCTATATCGAAATTACCCGTCGCGCACATCAGACATTGTGGCGAGAATATGAg < 2:495813/140‑1 (MQ=255) ggACGGCGATAACTGGGCCGATGACTCTCCGCTTTGCCATGAAATCCTGGCGAAAAAATTATTACCTGTTGTTCGTTATTACAGCTATATCAAAATTACCTGTCGCGCACATCAGACCTTGTTGCGAGAATATGAGCATc > 2:383840/1‑140 (MQ=255) cGATAACTGGGCCGATGACTCTCCGCTTTGCCATGAAATCCTGGCGAAAAAATTATTACCTGTTGTTCGTTATTACAGCTATATCGAAATTGCCCGTCGCGCACATCAGACATTGTGGCGAGGATATGAGCATCTGCAAt > 2:229881/1‑140 (MQ=255) cGATAACTGGGCCGATGACTCTCCGCTTTGCCATGAAATCCTGGCGAAAAAATTATTACCTGTTGTTCGTTATTACAGCTATATCGAAATTACCCGTCGCGCACATCAGACATTGTGGCGAGAATATGAGCATCTGCAAt > 2:569040/1‑140 (MQ=255) ggCCGATGACTCTCCGCTTTGCCATGAAATCCTGGCGAAAAAATTATTACCTGTTGTTCGTTATTACAGCTATATCGAAATTACCCGTCGCGCACATCAGACATTGTGGCGAGAATATGAGCATCTGCAATCTACTTTCg > 2:764110/1‑140 (MQ=255) gCCGATGACTCTCCGCTTTGCCATGAAATCCTGGCGAAAAAATTATTACCTGTTGTTCGTTATTACAGCTATATCGAAATTACCCGTCGCGCACATCAGACATTGTGGCGAGAATATGAGCATCTGCAATCTACTTTCGa < 1:764110/140‑1 (MQ=255) gCCGATGACTCTCCGCTTTGCCATGAAATCCTGGCGAAAAAATTATTACCTGTTGTTCGTTATTACAGCTATATCGAAATTACCCGTCGCGCACATCAGACATTGTGGCGAGAATATGAGCATCTGCAATCTACTTTCGa > 1:111033/1‑140 (MQ=255) gATGACTCTCCGCTTTGCCATGAAATCCTGGCGAAAAAATTATTACCTGTTGTTCGTTATTACAGCTATATCGAAATTACCCGTCGCGCACATCAGACATTGTGGCGAGAATATGAGCATCTGCAATCTACTTTCGACaa > 2:112559/1‑140 (MQ=255) ctctCCGCTTTGCCACGAAATCCTGCCGACAAAATTATTACCTGTTGTTCGTTATTACAGCTATATCGAAATTACCCGTCGCGCACATCAGACATTGTGGCGAGAATATGAGCATCTGCAATCTACTTTCGACAACTTTg < 1:274987/140‑1 (MQ=255) tctcCGCTTTGCCATGAAATCCTGGCGAAAAAATTATTACCTGTTGTTCGTTATTACAGCTATATCGAAATTACCCGTCGCGCACATCAGACATTGTGGCTAGACTATTAGCATCTGGAATCTACTTTCGACAACTTTGc > 2:274987/1‑140 (MQ=255) tcCGCTTTGCCATGAAATCCTGGCGAAAAAATTATTACCTGTTGTTCGTTATTACAGCTATATCGAAATTACCCGTCGCGCACATCAGACATTGTGGCGAGAATGTAAGCATCTGCAATCTATATTCGACAACTTTGCGa > 2:397992/1‑140 (MQ=255) tcCGCTTTGCCATGAAATCCTGGCGAAAAAATTATTACCTGTTGTTCGTTATTACAGCTATATCGAAATTACCCGTCGCGCACATCAGACATTGTGGCGAGAATATGAGCATCTGCAATCTACTTTCGACAACTTTGCGa < 2:739581/140‑1 (MQ=255) tcCGCTTTGCCATGAAATCCTGGCGAAAAAATTATTACCTGTTGTTCGTTATTACAGCTATATCGAAATTACCCGTCGCGCACATCAGACATTGTGGCGAGAATATGAGCATCTGCAATCTACTTTCGACAACTTTGCGa < 2:687273/140‑1 (MQ=255) cGCTTTGCCATGAAATCCTGGCGAAAAAATTATTACCTGTTGTTCGTTATTACAGCTATATCGAAATTACCCGTCGCGCACATCAGACATTGTGGCGAGAATATGAGCATCTGCAATCTACTTTCGACAACTTTGCGATg < 1:523580/140‑1 (MQ=255) gCCATGAAATCCTGGCGAAAAAATTATTACCTGTTGTTCGTTATTACAGCTATATCGAAATTACCCGTCGCGCACATCAGACATTGTGGCGAGAATATGAGCATCTGCAATCTACTTTCGACAACTTTGCGATGCAGcac > 1:855683/1‑140 (MQ=255) gCCATGAAATCCTGGCGAAAAAATTATTACCTGTTGTTCGTTATTACAGCTATATCGAAATTACCCGTCGCGCACATCAGACATTGTGGCGAGAATATGAGCATCTGCAATCTACTTTCGACAACTTTGCGATGCAGcac > 2:782217/1‑140 (MQ=255) gCGAAAAAATTATTACCTGTTGTTCGTTATTACAGCTATATCGAAATTACCCGTCGCGCACATCAGACATTGTGGCGAGAATATGAGCATCTGCAATCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGACCAGga > 2:116787/1‑140 (MQ=255) cGAAAAAATTATTACCTGTTGTTCGTTATTACAGCTATATCGAAATTACCCGTCGCGCACATCAGACATTGTGGCGAGAATATGAGCATCTGCAATCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGACCAGgat < 2:835786/140‑1 (MQ=255) aaaaaaTTATTACCTGTTGTTCGTTATTACAGCTATATCGAAATTACCCGTCGCGCACATCAGACATTGTGGCGAGAATATGAGCATCTGCAATCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGACCAGgatga > 1:582422/1‑140 (MQ=255) attattACCTGTTGTTCGTTATTACAGCTATATCGAAATTACCCGTCGCGCACATCAGACATTGTGGCGAGAATATGAGCATCTGCAATCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGACCAGGATGATAttt < 1:117391/140‑1 (MQ=255) ccTGTTGTTCGTTATTACAGCTATATCGAAATTACCCGTCGCGCACATCAGACATTGTGGCGAGAATATGAGCATCTGCAATCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGACCAGGATGATATTTATCCGgt < 1:569040/140‑1 (MQ=255) ccTGTTGTTCGTTATTACAGCTATATCGAAATTACCCGTCGCGCACATCAGACATTGTGGCGAGAATATGAGCATCTGCAATCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGACCAGGATGATATTTATCCGgt < 1:782217/140‑1 (MQ=255) tgttgtTCGTTATTACAGCTATATCGAAATTACCCGTCGCGCACATCAGACATTGTGGCGAGAATATGAGCATCTGCAATCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGACCAGGATGATATTTATCCGgtgt < 1:116787/140‑1 (MQ=255) tgttCGTTATTACAGCTATATCGAAATTACCCGTCGCGCACATCAGACATTGTGGCGAGAATATGAGCATCTGCAATCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGACCAGGATGATATTTATCCGGTGTTcc < 2:855683/140‑1 (MQ=255) cGTTATTACAGCTATATCGAAATTACCCGTCGCGCACATCAGACATTGTGGCGAGAATATGAGCATCTGCAATCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGa > 2:544859/1‑140 (MQ=255) ttaCAGCTATATCGAAATTACCCGTCGCGCACATCAGACATTGTGGCGAGAATATGAGCATCTGCAATCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGt < 1:49045/140‑1 (MQ=255) cAGCTATATCGAAATTACCCGTCGCGCACATCAGACATTGTGGCGAGAATATGAGCATCTGCAATCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTc > 1:187178/1‑140 (MQ=255) aggCTATATCGAAATTACCCGTCGCGCACATCATACATTTTGGCGAGACTATGAGCATCTGCAATCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTc < 2:374740/138‑1 (MQ=255) aGCTATATCGAAATTACCCGTCGCGCACATCAGACATTGTGGCGAGAATATGAGCATCTGCAATCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCa < 2:523554/140‑1 (MQ=255) gCTATATCGAAATTACCCGTCGCGCACATCAGACATTGTGGCGAGAATATGAGCATCTGCAATCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCAt < 1:112559/140‑1 (MQ=255) atatCGAAATTACCCGTCGCGCACATCAGACATTGTGGCGAGAATATGAGCATCTGCAATCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATaaa > 2:547264/1‑140 (MQ=255) atatCGAAATTACCCGTCGCGCACATCAGACATTGTGGCGAGAATATGAGCATCTGCAATCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATaaa > 2:752639/1‑140 (MQ=255) atatCGAAATTACCCGTCGCGCACATCAGACATTGTGGCGAGAATATGAGCATCTGCAATCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGACCAGGATGATATTGATCAGGTGTTCCGTGAACTGTTTCATaaa > 1:307213/1‑140 (MQ=255) atCGAAATTACCCGTCGCGCACATCAGACATTGTGGCGAGAATATGAGCATCTGCAATCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGACCAGGATTATATTTAACCGTTGCTCCGTGAACTGTTTCATAAACa > 1:288171/1‑140 (MQ=255) aaaTTACCCGTCGCGCACATCAGACATTGTGGCGAGAATATGAGCATCTGCAATCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAAt > 2:668457/1‑140 (MQ=255) aTTACCCGTCGCGCACATCAGACATTGTGGCGAGAATATGAGCATCTGCAATCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGACCGGGATGATAATTAGCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGc > 2:243706/1‑140 (MQ=255) aTTACCCGTCGCGCACATCAGACATTGTGGCGAGAATATGAGCATCTGCAATCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGc > 2:717396/1‑140 (MQ=255) aTTACCCGTCGCGCACATCAGACATTGTGGCGAGAATATGAGCATCTGCAATCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGc > 2:841533/1‑140 (MQ=255) aTTACCCGTCGCGCACATCAGACATTGTGGCGAGAATATGAGCATCTGCAATCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGc > 1:746316/1‑140 (MQ=255) aTTACCCGTCGCGCACATCAGACATTGTGGCGAGAATATGAGCATCTGCAATCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGACCAGGATGATATTTATCCGGTGTTCCGTAAACTGTTTCATAAACAAAAtct > 1:374740/1‑138 (MQ=255) aCCCGTCGCGCACATCAGACATTGTGGCGAGAATATGAGCATCTGCAATCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAAc > 2:124905/1‑140 (MQ=255) tcgcGCACATCAGACATTGTGGGGAGAATATAAGCCTCTGCAATCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTa < 2:307213/140‑1 (MQ=255) tcgcGCACATCAGACATTGTGGCGAGAATATGAGCATCTGCAATCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAgata < 1:668457/140‑4 (MQ=255) tcgcGCACATCAGACATTGTGGCGAGAATATGAGCATCTGCAATCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTa < 2:835294/140‑1 (MQ=255) | CACAGTGGAATATTTACGCTGCACAAGCATCGGACGGCGATAACTGGGCCGATGACTCTCCGCTTTGCCATGAAATCCTGGCGAAAAAATTATTACCTGTTGTTCGTTATTACAGCTATATCGAAATTACCCGTCGTGCACATCAGACATTGTGGCGAGAATATGAGCATCTGCAATCTACTTTCGACAACTTTGCGATGCAGCACATCCGCGACCAGGATGATATTTATCCGGTGTTCCGTGAACTGTTTCATAAACAAAATGCAACAGCTA > NC_000913/1869958‑1870230 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |