Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,926,868	G→A	R658C (CGC→TGC)	ptrB ←	oligopeptidase B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,926,868	0	G	A	100.0%	145.3 / NA	43	R658C (CGC→TGC)	ptrB	oligopeptidase B
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (20/23);  total (20/23)

CATGAGCCCGGAGCTGCGTTTAACACTGAAACATTATCTGGAAAATACTTAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAATATTGCACCTGAGAATCGTGCAAACCGGT  >  NC_000913/1926731‑1927003                                                                                                                                          |                                                                                                                                        cATGAGCCCGGAGCTGCGTTTAACACTGAAACATTATCTGGAAAATACTTAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCGTATTCCACCGCGACGCCTTCGTACGATTTAAAGCAAc                                                                                                                                       >  1:367501/1‑140 (MQ=255) cATGAGCCCGGAGCTGCGTTTAACACTGAAACATTATCTGGAAAATACTTAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCAAc                                                                                                                                       >  1:132976/1‑140 (MQ=255) cATGAGCCCGGAGCTGCGTTTAACACTGAAACATTATCTGGAAAATACTTAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCAAc                                                                                                                                       >  1:793987/1‑140 (MQ=255)   tGAGCCCGGAGCTGCGTTTAACACTGAAACATTATCTGGAAAATACTTAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCAACCa                                                                                                                                     >  1:692867/1‑140 (MQ=255)    gAGCCCGGAGCTGCGTTTAACACTGAAACATTATCTGGAAAATACTTAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCAACCAg                                                                                                                                    >  1:759048/1‑140 (MQ=255)              cTGCGTTTAACACTGAAACATTATCTGGAAAATACTTAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCAACCAGATTTACCGcc                                                                                                                          <  1:741416/140‑1 (MQ=255)               tGCGTTTAACACTGAAATATTATCTGGAAAACACTTAGTTCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCAACCAGATTTACCGCCa                                                                                                                         <  2:267350/140‑1 (MQ=255)                 cGTTTAACACTGAAACATTATCTGGAAAATACTTAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGGCTTCGTATGATATAGAGCAACAAGATTTACCGCCATg                                                                                                                       >  1:267350/1‑140 (MQ=255)                        cacTGAAACATTATCTGGAAAATACTTAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCAACCAGATTTACCGCCATGGCCTGAg                                                                                                                <  2:575509/140‑1 (MQ=255)                           tGAAACATTATCTGGAAAATACTTAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCAACCAGATTTACCGCCATGGCCTGAGTcc                                                                                                             >  1:140814/1‑140 (MQ=255)                             aaaCATTATCTGGAAAATACTTAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCAACCAGATTTACCGCCATGGCCTGAGTCCAt                                                                                                           >  1:429138/1‑140 (MQ=255)                                    aTCTGGAAAATACTTAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCAACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTa                                                                                                    <  2:759048/140‑1 (MQ=255)                                    aTCTGGAAAATACTTAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCAACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTa                                                                                                    <  2:140814/140‑1 (MQ=255)                                       tGGAAAATACTTAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCAACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACag                                                                                                 <  1:129209/140‑1 (MQ=255)                                        ggAAAATACTTAGTCTGCAGGCGTAGCGGTTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCAACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACaga                                                                                                <  1:358517/140‑1 (MQ=255)                                        ggAAAATACTTAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCAACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACaga                                                                                                <  2:132976/140‑1 (MQ=255)                                         gAAAATACTTAGTCCGCGGGCGGAGCGGGTAATGTGCCCCGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCAACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACagag                                                                                               <  2:344640/140‑1 (MQ=255)                                             aTACTTAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCAACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAAt                                                                                           <  2:429138/140‑1 (MQ=255)                                                     tCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCAACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATg                                                                                   <  1:472526/140‑1 (MQ=255)                                                          aGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCAACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCAt                                                                              <  2:715260/140‑1 (MQ=255)                                                           ggCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCAACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATc                                                                             >  2:638788/1‑140 (MQ=255)                                                               tAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCAACCAGATTTACCGCCATGGCCTGAGTACATGTCGGTACAGAGCAATAAGAGATGGTCATCGGtt                                                                         >  1:243439/1‑140 (MQ=255)                                                                              ccTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCAACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAAt                                                          >  2:436953/1‑140 (MQ=255)                                                                               cTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCAACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGAAATAAAAGATGATCATCGGTTTTCAGCTCGCGCAAtt                                                         >  1:312203/1‑140 (MQ=255)                                                                                tGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCAACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTcg                                                                >  1:632140/1‑132 (MQ=255)                                                                                tGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCAACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTcg                                                                <  2:632140/132‑1 (MQ=255)                                                                                   gcCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCAACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGc                                                     >  1:180991/1‑140 (MQ=255)                                                                                               aGAAAAGCATATTCCATCGCTATGCCTTCGTCCGAGTTATAGCAACCAAATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGc                                         <  2:243439/140‑1 (MQ=255)                                                                                                 aaaaGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCAACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCg                                       <  2:23994/140‑1 (MQ=255)                                                                                                  aaaGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCAACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCgg                                      <  2:692867/140‑1 (MQ=255)                                                                                                      cATATTCCATCGCTACGTCTTCATACGATTTCAAGCAACTAGATTTACAGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCGTTTTGCCGGTTcc                                  <  2:367501/140‑1 (MQ=255)                                                                                                      cATATTCCATCGCTACGACTTCTTACGATTTTAAGCAACAAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTcc                                  <  2:312203/140‑1 (MQ=255)                                                                                                           tCCATCGCTACGCCTTCGTACGATTTAAAGCAACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCACGCGCAAATTATCGTCCCATTGTGCCGGTTCCCAata                             >  1:408701/1‑140 (MQ=255)                                                                                                              aTCGCTACGCCTTCGTACGATTTAAAGCAACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAATATTg                          <  2:793987/140‑1 (MQ=255)                                                                                                              aTCGCTACGCCTTCGTACGATTTAAAGCAACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAATATTg                          <  2:748858/140‑1 (MQ=255)                                                                                                                cGCTACGCCTTCGTACGATTTAAAGCAACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCACTTTGCCCGTTCCCAATATTGCa                        >  1:344428/1‑140 (MQ=255)                                                                                                                    aCGCCTTCGTACGATTTAAAGCAACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAATATTGCACCTg                    >  1:731318/1‑140 (MQ=255)                                                                                                                     cGCCTTCGTACGATTTAAAGCAACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAATATTg                          <  2:664738/133‑1 (MQ=255)                                                                                                                     cGCCTTCGTACGATTTAAAGCAACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAATATTg                          >  1:664738/1‑133 (MQ=255)                                                                                                                     cGCCTTCGTACGATTTAAAGCAACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAATATTGCACCTga                   >  2:717757/1‑140 (MQ=255)                                                                                                                       ccTTCGTACGATTTAAAGCAACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTTGCGCAATTCAGCGACCCATTTTGCCTGTTCACAATATTGCACCTgaga                 >  2:265186/1‑140 (MQ=255)                                                                                                                           cGTACGATTTAAAGCAACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGATCCATTTTGCCGGTTGCCAATATTGCACTTGAGAATCg             >  1:321119/1‑140 (MQ=255)                                                                                                                                  tttAAAGCAACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAATATTGCACCTGAGAATCGTGCAAAc      <  2:180991/140‑1 (MQ=255)                                                                                                                                      aaGCAACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAATATTGCACCTGAGAATCGTGCAAACCGGt  <  1:123121/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                        CATGAGCCCGGAGCTGCGTTTAACACTGAAACATTATCTGGAAAATACTTAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAATAAAAGATGGTCATCGGTTTTCAGCTCGCGCAATTTAGCGACCCATTTTGCCGGTTCCCAATATTGCACCTGAGAATCGTGCAAACCGGT  >  NC_000913/1926731‑1927003

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑