Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,928,733	A→G	V36A (GTC→GCC)	ptrB ←	oligopeptidase B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,928,733	0	A	G	100.0%	106.0 / NA	31	V36A (GTC→GCC)	ptrB	oligopeptidase B
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (13/18);  total (13/18)

TTTTGATGTAGGGCGCAGAAACTTCTCGTTGCGGAATGCGGTCGATGATTTCCTTTAAGATGCGATCCTGCAAGGCTTGTTGTGAGGCCATCACCCGATGACCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACA  >  NC_000913/1928599‑1928864                                                                                                                                       |                                                                                                                                    ttttGATGTAGGGCGCAGAAACTTCTCGTTGCGGAATGCGGTCGATGATTTCCTTTAAGATGCGATCCTGCAAGGCTTGTTGTGAGGCCATCACCCGATGGCCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGGCTTCt                                                                                                                                >  1:647311/1‑140 (MQ=255)           gggCGCAGAAACTTCTCGTTGCGGAATGCGGTCGATGATTTCCTTTAAGATGCGATCCTGCAAGGCTTGTTGTGAGGCCATCACCCGATGGCCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGGCTTCTGGCTGAGAAc                                                                                                                      >  2:11584/1‑140 (MQ=255)                        tctcGTTGCGGAATGCGGTCGATGATTTCCTTTAAGATGCGATCCTGCAAGGCTTGTTGTGAGGCCATCACCCGATGGCCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGGCTTCTGGCTGAGAACGCGTATCGTCCCg                                                                                                         <  2:215322/140‑1 (MQ=255)                         ctcGTTGCGGAATGCGGTCGATGATTTCCTTTAAGATGCGATCCTGCAAGGCTTGTTGTGAGGCCATCACCCGATGGCCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGGCTTCTGGCTGAGAACGCGTATCGTCCCGc                                                                                                        >  2:659488/1‑140 (MQ=255)                          tcGTTGCGGAATGCGGTCGATGATTTCCTTTAAGATGCGATCCTGCAAGGCTTGTTGTGAGGCCATCACCCGATGGCCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGGCTTCTGGCTGAGAACGCGTATCGTCCCGCa                                                                                                       <  1:670191/140‑1 (MQ=255)                            gTTGCGGAATGCGGTCGATGATTTCCTTTAAGATGCGATCCTGCAAGGCTTGTTGTGAGGCCATCACCCGATGGCCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGGCTTCTGGCTGAGAACGCGTATCGTCCCGCAGc                                                                                                     <  2:164907/140‑1 (MQ=255)                                           cGATGATTTCCTTTAAGATGCGATCCTGCAAGGCTTGTTGTGAGGCCATCACCCGATGGCCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGGCTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCg                                                                                      <  1:168524/140‑1 (MQ=255)                                                    ccTTTAAGATGCGATCCTGCAAGGCTTGTTGTGAGGCCATCACCCGATGGCCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGGCTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTa                                                                             <  1:485803/140‑1 (MQ=255)                                                            aTGCGATCCTGCAAGGCTTGTTGTGAGGCCATCACCCGATGGCCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGGCTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATg                                                                     <  2:647311/140‑1 (MQ=255)                                                              gCGATCCTGCAAGGCTTGTTGTGAGGCCATCACCCGATGGCCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGGCTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGaa                                                                   <  1:659488/140‑1 (MQ=255)                                                              gCGATCCTGCAAGGCTTGTTGTGAGGCCATCACCCGATGGCCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGGCTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGaa                                                                   <  1:11584/140‑1 (MQ=255)                                                               cGATCCTGCAAGGCTTGTTGTGAGGCCATCACCCGATGGCCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGGCTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAg                                                                  >  2:507620/1‑140 (MQ=255)                                                                      gCAAGGCTTGTTGTGAGGCCATCACCCGATGGCCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGGCTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATg                                                           >  2:445428/1‑140 (MQ=255)                                                                       cAAGGCTTGTTGTGAGGCCATCACCCGATGGCCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGGCTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATgg                                                          <  1:445428/140‑1 (MQ=255)                                                                            cTTGTTGTGAGGCCATCACCCGATGGCCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGGCTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTACTTAACGATGCGCGTATAGCCATGAAGCGTCATGGCGTgg                                                     >  2:387333/1‑140 (MQ=255)                                                                            cTTGTTGTGAGGCCATCACCCGATGGCCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGGCTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGAATCGCCATGTAGCGTCATGGCGTgg                                                     >  1:397000/1‑140 (MQ=255)                                                                            cTTGTTGTGAGGCCATCACCCGATGGCCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGGCTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATGATCGATGCGCGTATGGCCATGAAGCGTCATGGCGTgg                                                     >  1:329483/1‑140 (MQ=255)                                                                                      ggCCATCACCCGATGGCCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGGCTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATgcggg                                           <  1:661736/140‑1 (MQ=255)                                                                                          aTCACCCGATGGCCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGGCTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGggcggc                                       >  2:677053/1‑140 (MQ=255)                                                                                           tCACCCGATGGCCGTTACTATTGTCTTGTCGCAGGTAGTCCAGGGCTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCt                                      <  1:387333/140‑1 (MQ=255)                                                                                           tCACCCGATGGCCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGGCTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCt                                      <  1:607316/140‑1 (MQ=255)                                                                                            cACCCGATGGCCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGGCTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCtt                                     >  2:573613/1‑140 (MQ=255)                                                                                                               ttttCTTGTTGCAGGTAGTCCAGGGCTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTACCATGTTATTGtt                  >  2:527028/1‑140 (MQ=255)                                                                                                                      gttgCAGGTAGTCCAGGGCTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTg           >  1:35236/1‑140 (MQ=255)                                                                                                                         gCAGGTAGTCCAGGGCTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGtt        >  1:718886/1‑140 (MQ=255)                                                                                                                           aGGTAGTCCAGGGCTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGa      <  2:35236/140‑1 (MQ=255)                                                                                                                             gTAGTCCAGGGCTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGTGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGaaa    <  1:507620/140‑1 (MQ=255)                                                                                                                              tAGTCCAGGGCTTCTGGCTGAGAACGCGTATGGTCCCGCCGCCAGTAGGAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAAc   <  2:397000/140‑1 (MQ=255)                                                                                                                               ggTCCAGGGCTTCTGGCTGAGAACGCGTATCGTCCAGCATCCAGTAGTTATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACa  <  2:320959/139‑1 (MQ=255)                                                                                                                               aGTCCAGGGCTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACa  <  2:460362/140‑1 (MQ=255)                                                                                                                               aGTCCAGGGCTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACa  <  1:573613/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                    TTTTGATGTAGGGCGCAGAAACTTCTCGTTGCGGAATGCGGTCGATGATTTCCTTTAAGATGCGATCCTGCAAGGCTTGTTGTGAGGCCATCACCCGATGACCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACA  >  NC_000913/1928599‑1928864

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑