BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,947,257	A→C	D40E (GAT→GAG)	ruvC ←	crossover junction endodeoxyribonuclease RuvC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,947,257	0	A	C	100.0%	114.8 / NA	34	D40E (GAT→GAG)	ruvC	crossover junction endodeoxyribonuclease RuvC
Reads supporting (aligned to +/- strand): ref base A (0/0); new base C (14/20); total (14/20)

GCGCGCCTGGCCCAGTTTCAGGGCTGAGTCAGCGTTCTTTGCCATAAAGACTTGTTCAATGGCGAAATAATCAGGCTGGAACTGGGTGATGATTTCCGTCACGCCCGCATAGATGAGTTTCAGACGAGACGGTAAATCATCCACTTTGGTGCGGATGCATCCGCTACCCAGGTAGGACAGTTGCCTACCTACCTGGCGGATGACGCCGTAGCCGGTCACGCGCGAACCCGGATCAATGCCGAGAATAATAGCCATCACGCGTCTCCG  >  NC_000913/1947122‑1947388

gcgcgcCTGGCCCAGTTTCAGGGCTGAGTCAGCGTTCTTTGCCATAAAGACTTGTTCAATGGCGAAATAATCAGGCTGGAACTGGGTGATGATTTCCGTCACGCCCGCATAGATGAGTTTCAGACGAGACGGTAActcat                                                                                                                                 <  1:822368/140‑1 (MQ=255)

gcgcgcCTGGCCCAGTTCCAGGCCTGAGTCACCGTTCTTTGCCATAAACACTTGTTCAATGGCGAAATAATCAGGCTGGAACTGGGTGATGATTTCCGTCACGCCCGCATAGATGAGTTTCAGACGAGACGGTAActcat                                                                                                                                 <  2:244717/140‑1 (MQ=255)

                  cAGGGCTGAGTCAGCGTTCTTTGCCATAAAGACTTGTTCAATGGCGAAATAATCAGGCTGGAACTGGGTGATGATTTCCGTCACGCCCGCATAGATGAGTTTCAGACGAGACGGTAACTCATCCACTTTGGTGCGGATGc                                                                                                               <  2:9847/140‑1 (MQ=255)

                      gCTGAGTCAGCGTTCTTTGCCATAAAGACTTGTTCAATGGCGAAATAATCAGGCTGGAACTGGGTGATGATTTCCGTCACGCCCGCATAGATGAGTTTCAGACGAGACGGTAACTCATCCACTTTGGTGCGGATGCAAcc                                                                                                           <  1:60959/140‑1 (MQ=255)

                           gTCAGCGTTCTTTGCCATAAAGACTTGTTCAATGGCGAAATAATCAGGCTGGAACTGGGTGATGATTTCCGTCACGCCCGCATAGATGAGTTTCAGACGAGACGGTAACTCATCCACTTTGGTGCGGATGCATCCGCTAc                                                                                                      <  1:839666/140‑1 (MQ=255)

                           gTCAGCGTTCTTTGCCATAAAGACTTGTTCAATGGCGAAATAATCAGGCTGGAACTGGGTGATGATTTCCGTCACGCCCGCATAGATGAGTTTCAGACGAGACGGTAACTCATCCACTTTGGTGCGGATGCATCCGCTAc                                                                                                      <  1:678273/140‑1 (MQ=255)

                           gTCAGCGTTCTTTGCCATAAAGACTTGTTCAATGGCGAAATAATCAGGCTGGAACTGGGTGATGATTTCCGTCACGCCCGCATAGATGAGTTTCAGACGAGACGGTAACTCATCCACTTTGGTGCGGATGCATCCGCTAc                                                                                                      <  2:623462/140‑1 (MQ=255)

                               gCGTTCTTTGCCATAAAGACTTGTTCAATGGCGAAATAATCAGGCTGGAACTGGGTGATGATTTCCGTCACGCCCGCATAGATGAGTTTCAGACGAGACGGTAACTCATCCACTTTGGTGCGGATGCATCCGCTACCCAg                                                                                                  >  1:103887/1‑140 (MQ=255)

                                cGTTCTTTGCCATAAAGACTTGTTCAATGGCGAAATAATCAGGCTGGAACTGGGTGATGATTTCCGTCACGCCCGCATAGATGAGTTTCAGACGAGACGGTAACTCATCCACTTTGGAGCGGATGCATCCGCTACCCAgg                                                                                                 >  1:282237/1‑140 (MQ=255)

                                     tttGCCATAAAGACTTGTTCAATGGCGAAATAATCAGGCTGGAACTGGGTGATGATTTCCGTCACGCCCGCATAGATGAGTTTCAGACGAGACGGTAACTCATCCACTTTGGTGCGGATGCATCCGCTACCCAGGTAGGa                                                                                            <  2:147714/140‑1 (MQ=255)

                                          cATAAAGACTTGTTCAATGGCGAAATAATCAGGCTGGAACTGGGTGATGATTTCCGTCACGCCCGCATAGATGAGTTTCAGACGATACGGTAACTCATCCACTTTGGTGCGGATGCATCCGCTACCCAGGTAGGACAGtt                                                                                       >  2:138647/1‑140 (MQ=255)

                                             aaaGACTTGTTCAATGGCGAAATAATCAGGCTGGAACTGGGTGATGATTTCCGTCACGCCCGCATAGATGAGTTTCAGACGAGACGGTAACTCATCCACTTTGGTGCGGATGCATCCGCTACCCAGGTAGGACAGTTGcc                                                                                    <  1:851720/140‑1 (MQ=255)

                                               aGACTTGTTCAATGGCGGAATATTCAGGCTGGAACGGGGTGATGATTTCCGTCACGCCCGCATAGATGAGTTTCAGACGAGACGGTAACTCATCCACTTTGGTGCGGATGCATCCGCTACCCAGGTAGGACAGTTGccta                                                                                  <  2:282237/140‑1 (MQ=255)

                                                 aCTTGTTCAATGGCGAAATAATCAGGCTGGAACTGGGTGATGATTTCCGTCACGCCCGCATAGATGAGTTTCAGACGAGACGGTAACTCATCCACTTTGGTGCGGATGCATCCGCTACCCAGGTAGGACAGTTGcctacc                                                                                >  2:823354/1‑140 (MQ=255)

                                                   ttGTTCAATGGCGAAATAATCAGGCTGGAACTGGGTGATGATTTCCGTCACGCCCGCATAGATGAGTTTCAGACGAGACGGTAACTCATCCACTTTGGTGCGGATGCATCCGCTACCCAGGTAGGACAGTTGcctaccta                                                                              >  2:499083/1‑140 (MQ=255)

                                                    tGTTCAATGGCGAAATAATCAGGATGGAACTGGGTGATGATTTCCGTCACGCCCGCATAGATGAGTTTCAGACGAGACGGTAACTCATCCACTTTGGTGCGGATGCATCCGCTACCCAGGTAGGAc                                                                                           >  2:216402/1‑126 (MQ=255)

                                                    tGTTCAATGGCAAAATAATCAGGCTGGAACTGGGTGATGATTTCCGTCACGCCCGCATAGATGAGTTTCAGACGAGACGGTAACTCATCCACTTTGGTGCGGATGCATCCGCTACCCAGGTAGGAc                                                                                           <  1:216402/126‑1 (MQ=255)

                                                     gTTCAATGGCGAAATAATCAGGCTGGAACTGGGTGATGATTTCCGTCACGCCCGCATAGATGAGTTTCAGACGAGACGGTAACTCATCCACTTTGGTGCGGATGCATCCGCTACCCAGGTAGGACAGTTGCCtacctacc                                                                            <  1:107207/140‑1 (MQ=255)

                                                      ttCAATGGCGAAATAATCAGGCTGGAACTGGGTGATGATTTCCGTCACGCCCGCATAGATGAGTTTCAGACGAGACGGTAACTCATCCACTTTGGTGCGGATGCATCCGCTACCCAGGTAGGACAGTTGCCTacctacct                                                                           >  1:808193/1‑140 (MQ=255)

                                                             gCGAAATAATCAGGCTGGAACTGGGTGATGATTTCCGTCACGCCCGCATAGATGAGTTTCAGACGAGACGGTAACTCATCCACTTTGGTGCGGATGCATCCGCTACCCAGGTAGGACAGTTGCCTACCTACCTGGCGGAt                                                                    <  2:103887/140‑1 (MQ=255)

                                                                aaaTAATCAGGCTGGAACTGGGTGATGATTTCCGTCACGCCCGCATAGATGAGTTTCAGACGAGACGGTAACTCATCCACTTTGGTGCGGATGCATCCGCTACCCAGGTAGGACAGTTGCCTACCTACCTGGCGGATGAc                                                                 >  1:736579/1‑140 (MQ=255)

                                                                 aataatCAGGCTGGAACTGGGTGATGATTTCCGTCACGCCCGCATAGATGAGTTTCAGACGAGACGGTAACTCATCCACTTTGGTGCGGATGCATCCGCTACCCAGGTAGGACAGTTGCCTACCTACCTGGCGGATGACg                                                                <  2:736579/140‑1 (MQ=255)

                                                                          gCTGGAACTGGGTGATGATTTCCGTCACGCCCGCATAGATGAGTTTCAGACGAGACGGTAACTCATCCACTTTGGTGCGGATGCATCCGCTACCCAGGTAGGACAGTTGCCTACCTACCTGGCGGATGACGCCGTAGCCg                                                       >  2:691397/1‑140 (MQ=255)

                                                                                  tGGGTGATGATTTCCGTCACGCCCGCATAGATGAGTTTCAGACGAGACGGTAACTCATCCACTTTGGTGCGGATGCATCCGCTACCCAGGTAGGACAGTTGCCTACCTACCTGGCGGATGACGCCGTAGCCGGTCAcgcg                                               <  1:138647/140‑1 (MQ=255)

                                                                                  tGGGTGATGATTTCCGTCACGCCCGCATAGATGAGTTTCAGACGAGACGGTAACTCATCCACTTTGGTGCGGATGCATCCGCTACCCAGGTAGGACAGTTGCCTACCTACCTGGCGGATGACGCCGTAGCCGGTCAcgcg                                               <  2:163365/140‑1 (MQ=255)

                                                                                    ggTGATGATTTCCGTCACGCCCGCATAGATGAGTTTCAGACGAGACGGTAACTCATCCACTTTGGTGCGGATGCATCCGCTACCCAGGTAGGACAGTTGCCTACCTACCTGGCGGATGACGCCGTAGCCGGTCAcgcgcg                                             >  1:83199/1‑140 (MQ=255)

                                                                                    ggTGATGATTTCCGTCACGCCCGCATAGATGAGTTTCAGACGAGACGGTAACTCATCCACTTTGGTGCGGATGCATCCGCTACCCAGGTAGGACAGTTGCCTACCTACCTGGCGGATGACGCCGTAGCCGGTCAcgcg                                               <  2:82228/138‑1 (MQ=255)

                                                                                    ggTGATGATTTCCGTCACGCCCGCATAGATGAGTTTCAGACGAGACGGTAACTCATCCACTTTGGTGCGGATGCATCCGCTACCCAGGTAGGACAGTTGCCTACCTACCTGGCGGATGACGCCGTAGCCGGTCAcgcg                                               >  1:82228/1‑138 (MQ=255)

                                                                                    ggTGATGATTTCCGTCACGCCCGCATAGATGAGTTTCAGACGAGACGGTAACTCATCCACTTTGGTGCGGATGCATCCGCTACCCAGGTAGGACAGTTGCCTACCTACCTCGCGCATGACGCCGCAGCCGGTCAcgcgcg                                             >  1:362298/1‑140 (MQ=255)

                                                                                        atgatTTCCGTCACGCCCGCATAGATGAGTTTCAGACGAGACGGTAACTCATCCACTTTGGTGCGGATGCATCCGCTACCCAGGTAGGACAGTTGCCTACCTACCTGGCGGATGACGCCGTAGCCGGTCACGCGCGATcc                                         <  2:83199/140‑1 (MQ=255)

                                                                                          gatTTCCGTCACGCCCGCATAGATGAGTTTCAGACGAGACGGTAACTCATCCACTTTGGTGCGGATGCATCCGCTACCCAGGTAGGACAGTTGCCTACCTACCTGGCGGATGACGCCGTAGCCGGTCACGCGCGAACCCg                                       <  1:823354/140‑1 (MQ=255)

                                                                                             ttCCGTCACGCCCGCATAGATGAGTTTCAGACGAGACGGTAACTCATCCACTTTGGTGCGGATGCATCCGCTACCCAGGTAGGACAGTTGCCTACCTACCTGGCGGATGACGCCGTAGCCGGTCACGCGCGAACCCGGAt                                    <  2:176386/140‑1 (MQ=255)

                                                                                               ccGTCACGCCCGCATAGATGAGTTTCAGACGAGACGGTAACTCATCCACTTTGGTGCGGATGCATCCGCTACCCAGGTAGGACAGTTGCCTACCTACCTGGCGGATGACGCCGTAGCCGGTCACGCGCGAACCCGGATCa                                  <  1:499083/140‑1 (MQ=255)

                                                                                                          gCATAGATGAGTTTCAGCCGAGTCGGTAACTTATCAACTTTGGTGCGGATGCATCCGCTACCCAGGTAGGACAGTTGCCTACCTAGCTGGCGGATGACGCCGTAGCCGGTCACGCGCGAACCCGGATCAATGCCGAGaat                       <  2:362298/140‑1 (MQ=255)

                                                                                                                          gacgagacgGTAACTCATCCACTTTGGTGCGGATGCATCCGCTACCCAGGTAGGACAGTTGCCTACCTACCTGGCGGATGACGCCGTAGCCGGTCACGCGCGAACCCGGATCAATGCCGAGAATAATAGCCATCACGCGt       >  1:11159/1‑140 (MQ=255)

                                                                                                                               gacgGTAACTCATCCACTTTGGTGCGGATGCATCCGCTACCCAGGTAGGACAGTTGCCTACCTACCTGGCGGATGACGCCGTAGCCGGTCAAGCGCGAACCCGGCTCACTGCCGAGAATAATTGCCATCACCCGTCTCCg  >  2:407767/1‑140 (MQ=255)

GCGCGCCTGGCCCAGTTTCAGGGCTGAGTCAGCGTTCTTTGCCATAAAGACTTGTTCAATGGCGAAATAATCAGGCTGGAACTGGGTGATGATTTCCGTCACGCCCGCATAGATGAGTTTCAGACGAGACGGTAAATCATCCACTTTGGTGCGGATGCATCCGCTACCCAGGTAGGACAGTTGCCTACCTACCTGGCGGATGACGCCGTAGCCGGTCACGCGCGAACCCGGATCAATGCCGAGAATAATAGCCATCACGCGTCTCCG  >  NC_000913/1947122‑1947388

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`