Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,986,976	T→C	F18L (TTT→CTT)	ftnB →	putative ferritin‑like protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,986,976	0	T	C	100.0%	150.9 / NA	44	F18L (TTT→CTT)	ftnB	putative ferritin‑like protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (34/10);  total (34/10)

CAGAAAGGAATTATTCTGCAAACAGTAATTATGGTGTTTTGATTTATCTTGCACCTCTCCACTTCTGGATATAAGGATATTAGGTATGGCAACCGCTGGAATGCTTCTCAAACTCAACTCTCAAATGAACCGCGAGTTTTACGCATCCAATCTCTACCTTCACCTGAGTAACTGGTGTTCTGAACAGAGTCTGAACGGCACCGCCACTTTCCTTCGCGCCCAGGCACAGAGTAATGTGACCCAAATGATGCGCATGTTTAACTTTATGAAGAGTGT  >  NC_000913/1986840‑1987115                                                                                                                                         |                                                                                                                                            cAGAAAGGAATTATTCTGCAAACAGTAATTATGGTGTTTTGATTTATCTTGCACCTCTCCACTTCTGGATATAAGGATATTAGGTATGGCAACCGCTGGAATGCTTCTCAAACTCAACTCTCAAATGAACCGCGAGcttt                                                                                                                                          >  2:663218/1‑140 (MQ=255)        gAATTATTCTGCAAACAGTAATTATGGTGTTTTGATTTATCTTGCACCTCTCCACTTCTGGATATAAGGATATTAGGTATGGCAACCGCTGGAATGCTTCTCAAACTCAACTCTCAAATGAACCGCGAGCTTTACGCATc                                                                                                                                   <  1:469837/140‑1 (MQ=255)                   cAAACAGTAATTATGGTGTTTTGATTTATCTTGCACCTCTCCACTTCTGGATATAAGGATATTAGGTATGGCAACCGCTGGAATGCTTCTCAAACTCAACTCTCAAATGAACCGCGAGCTTTACGCATCCAATCTCTAcc                                                                                                                        >  1:726126/1‑140 (MQ=255)                   cAAACAGTAATTATGGTGTTTTGATTTATCTTGCACCTCTCCACTTCTGGATATAAGGATATTAGGTATGGCAACCGCTGGAATGCTTCTCAAACTCAACTCTCAAATGAACCGCGAGCTTTACGCATCCAATCTCTAcc                                                                                                                        >  2:207572/1‑140 (MQ=255)                     aaCAGTAATTATGGTGTTTTGATTTATCTTGCACCTCTCCACTACTGGATATAAGGATATTAGGTATGGCAACCGCTGGAATGCTTCTCAAACTCAACTCTCAAATGAACCGCGAGCTTTACGCGGCCAATCTCTACCtt                                                                                                                      <  2:762255/140‑1 (MQ=255)                         gTAATTATGGTGTTTTGATTTATCTTGCACCTCTCCACTTCTGGATATAAGGATATTAGGTATGGCAACCGCTGGAATGCTTCTCAAACTCAACTCTCAAATGAACCGCGAGCTTTACGCATCCAATCTCTACCTTCAcc                                                                                                                  <  1:207572/140‑1 (MQ=255)                             ttATGGTGTTTTGATTTATCTTGCACCTCTCCACTTCTGGATATAAGGATATTAGGTATGGCAACCGCTGGAATGCTTCTCAAACTCAACTCTCAAATGAACCGCGAGCTTTACGCATCCAATCTCTACCTTCACCTGAg                                                                                                              >  1:853741/1‑140 (MQ=255)                                 ggTGTTTTGATTTATCTTGCACCTCTCCACTTCTGGATATAAGGATATTAGGTATGGCAACCGCTGGAATGCTTCTCAAACTCAACTCTCAAATGAACCGCGAGCTTTACGCATCCAATCTCTACCTTCACCTGAGTAAc                                                                                                          >  1:638208/1‑140 (MQ=255)                                  gtgtTTTGATTTATCTTGCACCTCTCCACTTCTGGATATAAGGATATTAGGTATGGCAACCGCTGGAATGCTTCTCAAACTCAACTCTCAAATGAACCGCGAGCTTTACGCATCCAATCTCTACCTTCACCTGAGTAACt                                                                                                         >  1:861233/1‑140 (MQ=255)                                   tgtTTTGATTTATCTTGCACCTCTCCACTTCTGGATATAAGGATATTAGGTATGGCAACCGCTGGAATGCTTCTCAAACTCAACTCTCAAATGAACCGCGAGCTTTACGCATCCAATCTCTACCTTCACCTGAGTAACTg                                                                                                        <  1:2072/140‑1 (MQ=255)                                    gtTTTGATTTATCTTGCACCTCTCCACTTCTGGATATAAGGATATTAGGTATGGCAACCGCTGGAATGCTTCTCAAACTCAACTCTCAAATGAACCGCGAGCTTTACGCATCCAATCTCTACCTTCACCTGAGTAACTgg                                                                                                       >  1:609557/1‑140 (MQ=255)                                     ttttGATTTATCTTGCACCTCTCCACTTCTGGATATAAGGATATTAGGTATGGCAACCGCTGGAATGCTTCTCAAACTCAACTCTCAAATGAACCGCGAGCTTTACGCATCCAATCTCTACCTTCACCTGAGTAACTGgt                                                                                                      <  2:853741/140‑1 (MQ=255)                                      tttGATTTATCTTGCACCTCTCCACTTCTGGATATAAGGATATTAGGTATGGCAACCGCTGGAATGCTTCTCAAACTCAACTCTCAAATGAACCGCGAGCTTTACGCATCCAATCTCTACCTTCACCTGAGTAACTGgtg                                                                                                     >  1:192859/1‑140 (MQ=255)                                      tttGATTTATCTTGCACCTCTCCACTTCTGGATATAAGGATATTAGGTATGGCAACCGCTGGAATGCTTCTCAAACTCAACTCTCAAATGAACCGCGAGCTTTACGCATCCAATCTCTACCTTCACCTGAGTAACTGgtg                                                                                                     >  1:797918/1‑140 (MQ=255)                                       ttGATTTATCTTGCACCTCTCCACTTCTGGATATAAGGATATTAGGTATGGCAACCGCTGGAATGCTTCTCAAACTCAACTCTCAAATGAACCGCGAGCTTTACGCATCCAATCTCTACCTTCACCTGAGTAACTGgtgt                                                                                                    >  1:698427/1‑140 (MQ=255)                                             tATCTTGCACCTCTCCACTTCTGGATATAAGGATATTAGGTATGGCAACCGCTGGAATGCTTCTCAAACTCAACTCTCAAATGAACCGCGAGCTTTACGCATCCAATCTCTACCTTCACCTGAGTAACTGGTGTTCTGaa                                                                                              >  1:182414/1‑140 (MQ=255)                                             tATCTTGCACCTCTCCACTTCTGGATATAAGGATATTAGGTATGGCAACCGCTGGAATGCTTCTCAAACTCAACTCTCAAATGAACCGCGAGCTTTACGCATCCAATCTCTACCTTCACCTGAGTAACTGGTGTTCTGaa                                                                                              <  2:609557/140‑1 (MQ=255)                                              aTCTTGCACCTCTCCACTTCTGGATATAAGGATATTAGGTATGGCAACCGCTGGAATGCTTCTCAAACTCAACTCTCAAATGAACCGCGAGCTTTACGCATCCAATCTCTACCTTCACCTGAGTAACTGGTGTTCTGAAc                                                                                             <  2:638208/140‑1 (MQ=255)                                              aTCTTGCACCTCTCCACTTCTGGATATAAGGATATTAGGTATGGCAACCGCTGGAATGCTTCTCAAACTCAACTCTCAAATGAACCGCGAGCTTTACGCATCCAATCTCTACCTTCACCTGAGTAACTGGTGTTCTGAAc                                                                                             <  1:153810/140‑1 (MQ=255)                                                  tGCACCTCTCCACTTCTGGATATAAGGATATTAGGTATGGCAACCGCTGGAATGCTTCTCAAACTCAACTCTCAAATGAACCGCGAGCTTTACGCATCCAATCTCTACCTTCACCTGAGTAACTGGTGTTCTGAACagag                                                                                         >  1:121407/1‑140 (MQ=255)                                                    cACCTCTCCACTTCTGGATATAAGGATATTAGGTATGGCAACCGCTGGAATGCTTCTCAAACTCAACTCTCAAATGAACCGCGAGCTTTACGCATCCAATCTCTACCTTCACCTGAGTAACTGgt                                                                                                      >  1:583065/1‑125 (MQ=255)                                                    cACCTCTCCACTTCTGGATATAAGGATATTAGGTATGGCAACCGCTGGAATGCTTCTCAAACTCAACTCTCAAATGAACCGCGAGCTTTACGCATCCAATCTCTACCTTCACCTGAGTAACTGgt                                                                                                      <  2:583065/125‑1 (MQ=255)                                                       ctctCCACTTCTGGATATAAGGATATTAGGTATGGCAACCGCTGGAATGCTTCTCAAACTCAACTCTCAAATGAACCGCGAGCTTTACGCATCCAATCTCTACCTTCACCTGAGTAACTGGTGTTCTGAACAGAGTCTGa                                                                                    <  2:518343/140‑1 (MQ=255)                                                         ctcCACTTCTGGATATAAGGATATTAGGTATGGCAACCGCTGGAATGCTTCTCAAACTCAACTCTCAAATGAACCGCGAGCTTTACGCATCCAATCTCTACCTTCACCTGAGTAACTGGTGTTCTGAACAGAGTCTGAAc                                                                                  >  2:734696/1‑140 (MQ=255)                                                         ctcCACTTCTGGATATAAGGATATTAGGTATGGCAACCGCTGGAATGCTTCTCAAACTCAACTCTCAAATGAACCGCGAGCTTTACGCATCCAATCTCTACCTTCACCTGAGTAACTGGTGTTCTGAACAGAGTCTGAAc                                                                                  >  1:697207/1‑140 (MQ=255)                                                         ctcCACTTCTGGATATAAGGATATTAGGTATGGCAACCGCTGGAATGCTTCTCAAACTCAACTCTCAAATGAACCGCGAGCTTTACGCATCCAATCTCTACCTTCACCTGAGTAACTGGTGTTCTGAACAGAGTCTGAAc                                                                                  >  1:731551/1‑140 (MQ=255)                                                                tCTGGATATAAGTATATTAGGTATGCCACCAGCTGGAATGCTTCCCAAACTCAACCCTCAAATGAACCGCGGGCTTTCCGAATCCAGTCCCTCCCTTCACCTGAATAACTGGTGTTCTGAACAGAGTCTGAACGccgccg                                                                           <  2:120935/140‑1 (MQ=255)                                                                      taaaaGGATATTGGGTATGGCAACCGCTGGAATGCTTCTCAAACTCACCTCTAAAATAAACCGCGAGCTTTACGCATCCAATCTCTCCCTTCCCCTGAGTAACGGGTGTTCTGAACAGGGTCGGAACGGCACCGCCACtt                                                                     <  2:698427/137‑1 (MQ=255)                                                                              tatTAGGTATGGCAACCGCTGGAATGCTTCTCAAACTCAACTCTCAAATGAACCGCGAGCTTTACGCATCCAATCTCTACCTTCACCTGAGTAACTGGTGTTCTGAACAGAGTGCGAGCGGCACCGCCACTTTCCTTcgc                                                             >  1:279735/1‑140 (MQ=255)                                                                              tatTAGGTATGGCAACCGCTGGAATGCTTCTCAAACTCAACTCTCAAATGAACCGCGAGCTTTACGCATCCAATCTCTACCTTCACCTGAGTAACTGGTGTTCTGAACAGAGTCTGAACGGCACCGCCACTTTCCTTcgc                                                             >  1:607750/1‑140 (MQ=255)                                                                              tatTAGGTATGGCAACCGCTGGAATGCTTCTCAAACTCAACTCTCAAATGAACCGCGAGCTTTACGCATCCAATCTCTACCTTCACCTGAGTAACTGGTGTTCTGAACAGAGTCTGAACGGCACCACCACTTTCCTTcgc                                                             >  1:827379/1‑140 (MQ=255)                                                                                   ggTATGGCAACCGCTGGAATGCTTCTCAAACTCAACTCTCAAATGAACCGCGAGCTTTACGCATCCAATCTCTACCTTCACCTGAGTAACTGGTGTTCTGAACAGAGTCTGAACGGTACCGCCACTTTCCTTCGCGCCCa                                                        >  1:631299/1‑140 (MQ=255)                                                                                   ggTATGGCAACCGCTGGAATGCTTCTCAAACTCAACTCTCAAATGAACCGCGAGCTTTACGCATCCAATCTCTACCTTCACCTGAGTAACTGGTGTTCTGAACAGAGTCTGAACGGCACCGCCACTTTCCTTCGCGCCCa                                                        >  1:42064/1‑140 (MQ=255)                                                                                   ggTATGGCAACCGCTGGAATGCTTCTCAAACTCAACTCTCAAATGAACCGCGAGCTTTACGCATCCAATCTCTACCTTCACCTGAGTAACTGGTGTTCTGAACAGAGTCTGAACGGCACCGCCACTTTCCTTCGCGCCCa                                                        >  1:626428/1‑140 (MQ=255)                                                                                   ggTATGGCAACCGCTGGAATGCTTCTCAAACTCAACTCTCAAATGAACCGCGAGCTTTACGCATCCAATCTCTACCTTCACCTGAGTAACTGGTGTTCTGAACAGAGTCTCAACCGCTCCGCCATTTTCCTTCGCGCCCa                                                        >  1:288636/1‑140 (MQ=255)                                                                                       tGGCAACCGCTGGAATGCTTCTCAAACTCAACTCTCAAATGAACCGCGAGCTTTACGCATCCAATCTCTACCTTCACCTGAGTAACTGGTGTTCTGAACAGAGTCTGAACGGCACCGCCACTTTCCTTCGCGCCCAGGca                                                    >  1:129257/1‑140 (MQ=255)                                                                                       tGGCAACCGCTGGAATGCTTCTCAAACTCAACTCTCAAATGAACCGCGAGCTTTACGCATCCAATCTCTACCTTCACCTGAGTAACTGGTGTTCTGAACAGAGTCTGAACGGCACCGCCACTTTCCTTCGCGCCCAGGca                                                    >  2:796363/1‑140 (MQ=255)                                                                                       tGGCAACCGCTGGAATGCTTCTCAAACTCAACTCTCAAATGAACCGCGAGCTTTACGCATCCAATCTCTACCTTCACCTGAGTAACTGGTGTTCTGAACAGAGTCTGAACGGCACCGCCACTTTCCTTCGCGCCCAGGca                                                    >  2:682391/1‑140 (MQ=255)                                                                                       gggCACCCGCTGGACTCCTTCTAAAACTCAACTCTCAAAGGACCGGCGGGTTTTACGCTCCCACCCCCTACCTTCCCTTGAGTAACGGGTGTTTTAAACAGAGTCTGAAAGCCACCGCCACTTTCCTTCGCGCCCAGGca                                                    <  2:797918/139‑1 (MQ=255)                                                                                         gCACCCGCGGGAATGCTTCACAACCTCAACTCAAAAATGACCCGCGAGCTTTACGCATCAAATCTCTCCCTCCCCCTGAGTAACTGGTGTTCTGAAAAGAGTCTGAACGGCACCGCCACTTTCCTTCGCGCCCAGGcaca                                                  <  2:626428/140‑1 (MQ=255)                                                                                          cAACCGCTGGAATGCTTCTCAAACTCAACTCTCAAATGAACCGCGAGCTTTACGCATCCAATCTCTACCTTCACCTGAGTAACTGGTGTTCTGAACAGAGTCTGAACGGCACCGCCACTTTCCTTCGCGCCCAGGCACag                                                 >  1:851600/1‑140 (MQ=255)                                                                                             ccGCTGGAATGCTTCTCAAACTCAACTCTCAAATGAACCGCGAGCTTTACGCATCCAATCTCTACCTTCACCTGAGTAACTGGTGTTCTGAACAGAGTCTGAACGGCACCGCCACTTTCCTTCGCGCCCAGGCACAGAGt                                              >  2:513550/1‑140 (MQ=255)                                                                                                  ggAATGCTTCTCAAACTCAACTCTCAAATGAACCGCGAGCTTTACGCATCCAATCTCTACCTTCACCTGAGTAACTGGTGTTCTGAACAGAGTCTGAACGGCACCGCCACTTTCCTTCGCGCCCAGGCACAGAGTAAtgt                                         >  2:479974/1‑140 (MQ=255)                                                                                                        cTTCTCAAACTCAACTCTCAAATGAACCGCGAGCTTTACGCATCCAATCTCTACCTTCACCTGAGTAACTGGTGTTCTGAACAGAGTCTGAACGGCACCGCCGCTTTCCTACGCACCGAGGCACGGAGTAATGTGACCCa                                   >  1:223046/1‑140 (MQ=255)                                                                                                        cTTCTCAAACTCAACTCTCAAATGAACCGCGAGCTTTACGCATCCAATCTCTACCTTCACCTGAGTAACTGGTGTTCTGAACAGAGTCTGAACGGCACCGCCACTTTCCTTCGCGCCCAGGCACAGAGTAATGTGACCCa                                   >  2:160923/1‑140 (MQ=255)                                                                                                                  caaacccTCAAATGAACCGCGAGCTTTACGCATCAAATCTCTCCCTCCCCCTGAGTAACTGGTGTTCTGAACAGAGTCTGAACGGCACCGCCACTTTCCTTCGCGCCCAGGCACAGAGTAATGTGACCCAAATGATgcgc                         <  2:851600/138‑1 (MQ=255)                                                                                                                    aactcTCAAATGAACCGCGAGCTTTACGCATCCAATCTCTACCTTCACCTGAGTAACTGGTGTTCTGAACAGAGTCTGAACGGCACCGCCACTTTCCTTCGCGCCCAGGCACAGAGTAATGTGACCCAAATGATGCGCAt                       >  1:782483/1‑140 (MQ=255)                                                                                                                                aaCCGCGAGCTTTACGCATCCAATCTCTACCTTCACCTGAGTAACTGGTGTTCTGAACAGAGTCTGAACGGCACCGCCACTTTCCTTCGCGCCCAGGCACAGAGTAATGTGACCGAAATGATGCGCATGTTTAACTTTAt           >  1:271221/1‑140 (MQ=255)                                                                                                                                 cccGCGAGCTTTACGCATCCAATCTCTCCCTTCCCCTGAGTAACTGGTGTTCTAAACAGAGTCTGAACGGCACCGCCACTTTCCTTCGCGCCCAGGCACAGAGTAATGTGACCCAAATGATGCGCATGTTTAACTTTATg          <  2:3095/139‑1 (MQ=255)                                                                                                                                     cgAGCTTTACGCATCCAATCTCTACCTTCACCTGAGTAACTGGTGTTCTGAACAGAGTCTGAACGGCACCGCCACTTTCCTTCGCGCCCAGGCACAGAGTAATGTGACCCAAATGATGCGCATGTTTAACTTTATGAaga      >  2:549390/1‑140 (MQ=255)                                                                                                                                     cgAGCTTTACGCATCCAATCTCTACCTTCACCTGAGTAACTGGTGTTCTGAACAGAGTCTGAACGGCACCGCCACTTTCCTTCGCGCCCAGGCACAGAGTAATGTGACCCAAATGATGCGCATGTTTAACTTTATGAaga      >  1:3095/1‑140 (MQ=255)                                                                                                                                         ttttCCGCATCCACTCTCTCCCTCCCCCTGAGTACGTGGTGTTCTGAAGAGAGTCAGAAGGGCTCCGCCACTTTCCTTCGCGCCCAGGCACAGAGTAATGTGACCCAAATGATGCGCATGTTTAACTTTATGAAGAgtgt  <  2:697207/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                            CAGAAAGGAATTATTCTGCAAACAGTAATTATGGTGTTTTGATTTATCTTGCACCTCTCCACTTCTGGATATAAGGATATTAGGTATGGCAACCGCTGGAATGCTTCTCAAACTCAACTCTCAAATGAACCGCGAGTTTTACGCATCCAATCTCTACCTTCACCTGAGTAACTGGTGTTCTGAACAGAGTCTGAACGGCACCGCCACTTTCCTTCGCGCCCAGGCACAGAGTAATGTGACCCAAATGATGCGCATGTTTAACTTTATGAAGAGTGT  >  NC_000913/1986840‑1987115

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑