Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,015,075	T→C	F66L (TTT→CTT)	fliG →	flagellar motor switch protein FliG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,015,075	0	T	C	100.0%	194.2 / NA	54	F66L (TTT→CTT)	fliG	flagellar motor switch protein FliG
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (21/33);  total (21/33)

GGCGGCAGAGGTGTTCAAGCACCTCTCCCAGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACGCAGATCTCCAACAAGCAGCTAACCGATGTGCTGGCGGAGTTTGAGCAAGAAGCTGAACAGTTTGCCGCACTGAATATCAACGCCAACGATTATCTGCGCTCGGTATTGGTCAAAGCTCTGGGTGAAGAACGTGCCGCCAGCCTGCTGGAAGATATTCTCGAAACTCGCGATACCGCCAGCGGTATTGAAACGCT  >  NC_000913/2014942‑2015208                                                                                                                                      |                                                                                                                                      ggcggcAGAGGTGTTCAAGCACCTCTCCCAGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACGCAGATCTCCAACAAGCAGCTAACCGATGTGCTGGCGGAGTTTGAGCAAGAAGCTGAACAGCTTGCCg                                                                                                                                 >  1:159817/1‑140 (MQ=255) ggcggcAGAGGTGTTCAAGCACCTCTCCCAGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACGCAGATCTCCAACAAGCAGCTAACCGATGTGCTGGCGGAGTTTGAGCAAGAAGCTGAACAGCTTGCCg                                                                                                                                 >  2:634178/1‑140 (MQ=255)   cggcAGAGGTGTTCAAGCACCTCTCCCAGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACGCAGATCTCCAACAAGCAGCTAACCGATGTGCTGGCGGAGTTTGAGCAAGAAGCTGAACAGCTTGCCGCa                                                                                                                               <  2:576105/140‑1 (MQ=255)   cggcAGAGGTGTTCAAGCACCTCTCCCAGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACGCAGATCTCCAACAAGCAGCTAACCGATGTGCTGGCGGAGTTTGAGCAAGAAGCTGAACAGCTTGCCGCa                                                                                                                               <  2:781670/140‑1 (MQ=255)     gcAGAGGTGTTCAAGCACCTCTCCCAGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACGCAGATCTCCAACAAGCAGCTAACCGATGTGCTGGCGGAGTTTGAGCAAGAAGCTGAACAGCTTGCCGCACt                                                                                                                             <  2:159817/140‑1 (MQ=255)      cAGAGGTGTTCAAGCACCTCTCCCAGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACGCAGATCTCCAACAAGCAGCTAACCGATGTGCTGGCGGAGTTTGAGCAAGAAGCTGAACAGCTTGCCGCACTg                                                                                                                            >  2:644579/1‑140 (MQ=255)         agGTGTTCAAGCACCTCTCCCAGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACGCAGATCTCCAACAAGCAGCTAACCGATGTGCTGGCGGAGTTTGAGCAAGAAGCTGAACAGCTTGCCGCACTGAat                                                                                                                         >  1:674612/1‑140 (MQ=255)            tgtTCAAGCACCTCTCCCAGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACGCAGATCTCCAACAAGCAGCTAACCGATGTGCTGGCGGAGTTTGAGCAAGAAGCTGAACAGCTTGCCGCACTGAATATc                                                                                                                      <  2:756375/140‑1 (MQ=255)               tCAAGCACCTCTCCCAGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACGCAGATCTCCAACAAGCAGCTAACCGATGTGCTGGCGGAGTTTGAGCAAGAAGCTGAACAGCTTGCCGCACTGAATATCAAc                                                                                                                   <  1:634178/140‑1 (MQ=255)                cAAGCACCTCTCCCAGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACGCAGATCTCCAACAAGCAGCTAACCGATGTGCTGGCGGAGTTTGAGCAAGAAGCTGAACAGCTTGCCGCACTGAATATCAACg                                                                                                                  >  1:181319/1‑140 (MQ=255)                  aGCACCTCTCCCAGCGTAAAGTCCAAACCCTAAGCGCTGCAATGGCGAACGTCACGCAGATCTCCAACAAGCAGCTAACCGATGTGCTGGCGGAGTTTGAGCAAGAAGCTGAACAGCTTGCCGCACTGAATATCAACGcc                                                                                                                <  2:352226/140‑1 (MQ=255)                   gCACCTCTCCCAGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACGCAGATCTCCAACAAGCAGCTAACCGATGTGCTGGCGGAGTTTGAGCAAGAAGCTGAACAGCTTGCCGCa                                                                                                                               >  1:209839/1‑124 (MQ=255)                   gCACCTCTCCCAGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACGCAGATCTCCAACAAGCAGCTAACCGATGTGCTGGCGGAGTTTGAGCAAGAAGCTGAACAGCTTGCCGCa                                                                                                                               <  2:209839/124‑1 (MQ=255)                             cAGCGTGAAGTACAAGCCCTGACCGGTGCAATGGCGAACGTCACGCAGATCTCCAACAAGCAGCTAACCGATGTGCTGGCGGAGTTTGAGCAAGAAGCTGAACAGCTTGCCGCACTGAATATCAACGCCAACGATTATCt                                                                                                     <  1:345345/140‑1 (MQ=255)                                 gTGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACGCAGATCTCCAACAAGCAGCTAACCGATGTGCTGGCGGAGTTTGAGCAAGAAGCTGAACAGCTTGCCGCACTGAATATCAACGCCAACGATTATCt                                                                                                     >  2:1435/1‑136 (MQ=255)                                 gTGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACGCAGATCTCCAACAAGCAGCTAACCGATGTGCTGGCGGAGTTTGAGCAAGAAGCTGAACAGCTTGCCGCACTGAATATCAACGCCAACGATTATCt                                                                                                     <  1:1435/136‑1 (MQ=255)                                  tGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACGCAGATCTCCAACAAGCAGCTAACCGATGTGCTGGCGGAGTTTGAGCAAGAAGCTGAACAGCTTGCCGCACTGAATATCAACGCCAACGATTATCTGCGCt                                                                                                <  1:658480/140‑1 (MQ=255)                                  tGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACGCAGATCTCCAACAAGCAGCTAACCGATGTGCTGGCGGAGTTTGAGCAAGAAGCTGAACAGCTTGCCGCACTGAATATCAACGCCAACGATTATCTGCGCt                                                                                                <  1:816024/140‑1 (MQ=255)                                  tGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACGCAGATCTCCAACAAGCAGCTAACCGATGTGCTGGCGGAGTTTGAGCAAGAAGCTGAACAGCTTGCCGCACTGAATATCAACGCCAACGATTATCTGCGCt                                                                                                <  2:181319/140‑1 (MQ=255)                                  tGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACGCAGATCTCCAACAAGCAGCTAACCGATGTGCTGGCGGAGTTTGAGCAAGAAGCTGAACAGCTTGCCGCACTGAATATCAACGCCAACGATTATCTGCGCt                                                                                                <  1:780594/140‑1 (MQ=255)                                  tGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACGCAGATCTCCAACAAGCAGCTAACCGATGTGCTGGCGGAGTTTGAGCAAGAAGCTGAACAGCTTGCCGCACTGAATATCAACGCCAACGATTATCTGCGCt                                                                                                <  1:644579/140‑1 (MQ=255)                                  tGAAGTACAAACCCTCAGCGCTCCAGTGGCGAACGTCTCGCAGATCTCCAACAAGCAGCTAACCGATGTGCTGGCGGAGTTTGAGCAAGAAGCTGAACAGCTTGCCGCACTGAATATCAACGCCAACGATTATCTGCGCt                                                                                                <  1:255700/140‑1 (MQ=255)                                    aaGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACGCAGATCTCCAACAAGCAGCTAACCGATGTGCTGGCGGAGTTTGAGCAAGAAGCTGAACAGCTTGCCGCACTGAATATCAACGCCAACGATTATCTGCGCt                                                                                                >  1:577223/1‑138 (MQ=255)                                    aaGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACGCAGATCTCCAACAAGCAGCTAACCGATGTGCTGGCGGAGTTTGAGCAAGAAGCTGAACAGCTTGCCGCACTGAATATCAACGCCAACGATTATCTGCGCt                                                                                                >  2:521240/1‑138 (MQ=255)                                    aaGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACGCAGATCTCCAACAAGCAGCTAACCGATGTGCTGGCGGAGTTTGAGCAAGAAGCTGAACAGCTTGCCGCACTGAATATCAACGCCAACGATTATCTGCGCt                                                                                                <  1:521240/138‑1 (MQ=255)                                    aaGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACGCAGATCTCCAACAAGCAGCTAACCGATGTGCTGGCGGAGTTTGAGCAAGAAGCTGAACAGCTTGCCGCACTGAATATCAACGCCAACGATTATCTGCGCt                                                                                                <  2:577223/138‑1 (MQ=255)                                       tACAAACCCTGAGCGCTGCAATGGCGAACGTCACGCAGATCTCCAACAAGCAGCTAACCGATGTGCTGGCGGAGTTTGAGCAAGAAGCTGAACAGCTTGCCGCACTGAATATCAACGCCAACGATTATCTGCGCTCGGTa                                                                                           <  2:674612/140‑1 (MQ=255)                                                 gAGCGCTGCAATGGCGAACGTCACGCAGATCTCCAACAAGCAGCTAACCGATGTGCTGGCGGAGTTTGAGCAAGAAGCTGAACAGCTTGCCGCACTGAATATCAACGCCAACGATTATCTGCGCTCGGTAt                                                                                          <  1:837598/131‑1 (MQ=255)                                                 gAGCGCTGCAATGGCGAACGTCACGCAGATCTCCAACAAGCAGCTAACCGATGTGCTGGCGGAGTTTGAGCAAGAAGCTGAACAGCTTGCCGCACTGAATATCAACGCCAACGATTATCTGCGCTCGGTAt                                                                                          >  2:837598/1‑131 (MQ=255)                                                   gcgcTGCAATGGCGAACGTCACTCATATCTCCAACAAGCAGCTAACCGATGTGCTGGCGGAGTTTGAGCAAGAAGCTGAACAGCTTGCCGCACTGAATATCAACGCCAACGATTATCTGCGCTCGGTATTGGTCAAAGct                                                                               <  1:373139/140‑1 (MQ=255)                                                   gcgcTGCAATGGCGAACGTCACGCAGATCTCCAACAAGCAGCTAACCGATGTGCTGGCGGAGTTTGAGCAAGAAGCTGAACAGCTTGCCGCACTGAATATCAACGCCAACGATTATCTGCGCTCGGTATTGGTCAAAGct                                                                               >  1:801356/1‑140 (MQ=255)                                                    cgcTGCAATGGCGAACGTCACGCAGATCTCCAACAAGCAGCTAACCGATGTGCTGGCGGAGTTTGAGCAAGAAGCTGAACAGCTTGCCGCACTGAATATCAACGCCAACGATTATCTGCGCTCGGTATTGGTCAAAGctc                                                                              <  2:46680/140‑1 (MQ=255)                                                    cgcTGCAATGGCGAACGTCACGCAGATCTCCAACAAGCAGCTAACCGATGTGCTGGCGGAGTTTGAGCAAGAAGCTGAACAGCTTGCCGCACTGAATATCAACGCCAACGATTATCTGCGCTCGGTATTGGTCAAAGctc                                                                              <  2:792627/140‑1 (MQ=255)                                                        gCAATGGCGAACGTCACGCAGATCTCCAACAAGCAGCTAACCGATGTGCTGGCGGAGTTTGAGCAAGAAGCTGAACAGCTTGCCGCACTGAATATCAACGCCAACGATTATCTGCGCTCGGTATTGGTCAAAGCTCTggg                                                                          >  2:147999/1‑140 (MQ=255)                                                                   cGTCACGCAGATCTCCAACAAGCAGCTAACCGATGTGCTGGCGGAGTTTGAGCAAGAAGCTGAACAGCTTGCCGCACTGAATATCAACGCCAACGATTATCTGCGCTCGGTATTGGTCAAAGCTCTGGGTGAAGAACGTg                                                               >  2:43215/1‑140 (MQ=255)                                                                   cGTCACGCAGATCTCCAACAAGCAGCTAACCGATGTGCTGGCGGAGTTTGAGCAAGAAGCTGAACAGCTTGCCGCACTGAATATCAACGCCAACGATTATCTGCGCTCGGAATTGGTCAAAGCTCTGGGTGAAGAACGTg                                                               >  1:315435/1‑140 (MQ=255)                                                                         gCAGATCTCCAACAAGCAGCTAACCGATGTGCTGGCGGAGTTTGAGCAAGAAGCTGAACAGCTTGCCGCACTGAATATCAACGCCAACGATTATCTGCGCTCGGTATTGGTCAAAGCTCTGGGTGAAGAACGTGCCGCCa                                                         <  2:801356/140‑1 (MQ=255)                                                                               ctcCAACAAGCAGCTAACCGATGTGCTGGCGGAGTTTGAGCAAGAAGCTGAACAGCTTGCCGCACTGAATATCAACGCCAACGATTATCTGCGCTCGGTATTGGTCAAAGCTCTGGGTGAAGAACGTGCCGCCAGCctgc                                                   >  2:755035/1‑140 (MQ=255)                                                                                       agcagcTAACCGATGTGCTGGCGGAGTTTGAGCAAGAAGCTGAACAGCTTGCCGCACTGAATATCAACGCCAACGATTATCTGCGCTCGGTATTGGTCAAAGCTCTGGGTGAAGAACGTGCCGCCAGc                                                       >  2:852513/1‑128 (MQ=255)                                                                                       agcagcTAACCGATGTGCTGGCGGAGTTTGAGCAAGAAGCTGAACAGCTTGCCGCACTGAATATCAACGCCAACGATTATCTGCGCTCGGTATTGGTCAAAGCTCTGGGTGAAGAACGTGCCGCCAGc                                                       <  1:852513/128‑1 (MQ=255)                                                                                         cagcTAACCGATGTGCTGGCGGAGTTTGAGCAAGAAGCTGAACAGCTTGCCGCACTGAATATCAACGCCAACGATTATCTGCGCTCGGTATTGGTCAAAGCTCTGGGTGAAGAACGTGCCGCCAGCCTGCTGGAAGatat                                         >  1:573154/1‑140 (MQ=255)                                                                                         cagcTAACCGATGTGCTGGCGGAGTTTGAGCAAGAAGCTGAACAGCTTGCCGCACTGAATATCAACGCCAACGATTATCTGCGCTCGGTATTGGTCAAAGCTCTGGGTGAAGAACGTGCCGCCAGCCTGCTGGAAGatat                                         >  1:216511/1‑140 (MQ=255)                                                                                         cagcTAACCGATGTGCTGGCGGAGTTTGAGCAAGAAGCTGAACAGCTTGCCGCACTGAATATCAACGCCAACGATTATCTGCGCTCGGTATTGGTCAAAGCTCTGGGTGAAGAACGTGCCGCCAGCCTGCTGGAAGatat                                         >  2:849214/1‑140 (MQ=255)                                                                                           gcTAACCGATGTGCTGGCGGAGTTTGAGCAAGAAGCTGAACAGCTTGCCGCACTGAATATCAACGCCAACGATTATCTGCGCTCGGTATTGGTCAAAGCTCTGGGTGAAGAACGTGCCGCCAGCCTGCTGGAAGATATtc                                       >  2:752677/1‑140 (MQ=255)                                                                                              aaCCGATGTGCTGGCGGAGTTTGAGCAAGAAGCTGAACAGCTTGCCGCACTGAATATCAACGCCAACGATTATCTGCGCTCGGTATTGGTCAAAGCTCTGGGGGAAGAACGTGCCGCGAGCCTGATGGAAGATATTCTCg                                    >  1:262162/1‑140 (MQ=255)                                                                                                         tGGCGGAGTTTGAGCAAGAAGCTGAACAGCTTGCCGCACTGAATATCAACGCCAACGATTATCTGCGCCCGGTATTGGTCAAAGCTCTGGGTGAAGAACGTGCCGCCAGCCTGCTGGAAGATATTCTCGAAACTCGCGAt                         <  2:216511/140‑1 (MQ=255)                                                                                                          ggCGGAGTTTGAGCAAGAAGCTGAACAGCTTGCCGCACTGAATATCAACGCCAACGATTATCTGCGCTCGGTATTGGTCAAAGCTCTGGGTGAAGAACGTGCCGCCAGCCTGCTGGAAGATATTCTCGAAACTCGCGATa                        <  2:158300/140‑1 (MQ=255)                                                                                                                 tttGAGCAAGAAGCTGAACAGCTTGCCGCACTGAATATCAACGCCAACGATTATCTGCGCTCGGTATTGGTCAAAGCTCTGGGTGAAGAACGTGCCGCCAGCCTGCTGGAAGATATTCTCGAAACTCGCGATACCGCCAg                 <  1:562142/140‑1 (MQ=255)                                                                                                                 tttGAGCAAGAAGCTGAACAGCTTGCCGCACTGAATATCAACGCCAACGATTATCTGCGCTCGGTATTGGTCAAAGCTCTGGGTGAAGAACGTGCCGCCAGCCTGCTGGAAGATATTCTCGAAACTCGCGATACCGCCAg                 <  1:43215/140‑1 (MQ=255)                                                                                                                 tttGAGCAAGAAGCTGAACAGCTTGCCGCACTGAATATCAACGCCAACGATTATCTGCGCTCGGTATTGGTCAAAGCTCTGGGTGAAGAACGTGCCGCCAGCCTGCTGGAAGATATTCTCGAAACTCGCGATACCGCCAg                 <  1:147999/140‑1 (MQ=255)                                                                                                                     aGCATGAAGCTGAACAGCTTGCCGCACTGAATATCAACGCCTATGTTTATCTGCGCTCGGTATTGGTCAAAGCTCTGGGTGAAGAACGTGCCGCCAGCCTTGTGGACGACATTCTCGAAACTCACGATACCGCCAGCGGt             >  2:420177/1‑140 (MQ=255)                                                                                                                           aagCTGAACAGCTTGCCGCACTGAATATCAACGCCAACGATTATCTGCGCTCGGTATTGGTCAAAGCTCTGGGTGAAGAACGTGCCGCCAGCCTGCTGGAAGATATTCTCGAAACTCGCGATACCGCCAGCGGTATTGaa       <  1:755035/140‑1 (MQ=255)                                                                                                                                gAACAGCTTGCCGCACTGAATATCAACGCCAACGATTATCTGCGCTCGGTATTGGTCAAAGCTCTGGGTGAAGAACGTGCCGCCAGCCTGCTGGAAGATATTCTCGAAACTCGCGATACCGCCAGCGGTATTGAAACGCt  <  2:573154/140‑1 (MQ=255)                                                                                                                                gAACAGCTTGCCGCACTGAATATCAACGCCAACGATTATCTGCGCTCGGTATTGGTCAAAGCTCTGGGTGAAGAACGTGCCGCCAGCCTGCTGGAAGATATTCTCGAAACTCGCGATACCGCCAGCGGTATTGAAACGCt  <  1:849214/140‑1 (MQ=255)                                                                                                                                gAACAGCTTGCCGCACTGAATATCAACGCCAACGATTATCTGCGCTCGGTATTGGTCAAAGCTCTGGGTGAAGAACGTGCCGCCAGCCTGCTGGAAGATATTCTCGAAACTCGCGATACCGCCAGCGGTATTGAAACGCt  <  1:112469/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                                      GGCGGCAGAGGTGTTCAAGCACCTCTCCCAGCGTGAAGTACAAACCCTGAGCGCTGCAATGGCGAACGTCACGCAGATCTCCAACAAGCAGCTAACCGATGTGCTGGCGGAGTTTGAGCAAGAAGCTGAACAGTTTGCCGCACTGAATATCAACGCCAACGATTATCTGCGCTCGGTATTGGTCAAAGCTCTGGGTGAAGAACGTGCCGCCAGCCTGCTGGAAGATATTCTCGAAACTCGCGATACCGCCAGCGGTATTGAAACGCT  >  NC_000913/2014942‑2015208

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑