| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,017,886 | T→C | S445P (TCT→CCT) | fliI → | flagellum‑specific ATP synthase FliI |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,017,886 | 0 | T | C | 97.1% | 110.0 / NA | 34 | S445P (TCT→CCT) | fliI | flagellum‑specific ATP synthase FliI |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (12/21); minor base A (1/0); total (13/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.82e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01 | |||||||||||
TAACCGCGATCTGGTTAGCGTCGGCGCGTATGCCAAAGGCAGCGATCCGATGCTCGATAAAGCCATCGCCCTGTGGCCGCAGCTGGAGGGCTATTTGCAACAAGGCATTTTTGAACGCGCGGACTGGGAAGCGTCTCTCCAGGGGCTGGAGCGTATTTTCCCGACAGTGTCATAACCCAGGAGATAACGGCAGATGGCAGAACATGGTGCGCTGGCGACCCTGAAAGATCTGGCAGAAAAAGAGGTAGAGGATGCCGCGC > NC_000913/2017753‑2018012 | tAACCGCGATCTGGTTAGCGTCGGCGCGTATGCCAAAGGCAGCGATCCGATGCTCGATAAAGCCATCGCCCTGTGGCCGCAGCTGGAGGGCTATTTGCAACAAGGCATTTTTGAACGCGCGGACTGGGAAGCGCCTCTcc < 1:800712/140‑1 (MQ=255) aTCTGGTTAGCGTCGGCGCGTATGCCAAAGGCAGCGATCCGATGCTCGATAAAGCCATCGCCCTGTGGCCGCAGCTGGAGGGCTATTTGCAACAAGGCATTTTTGAACGCGCGGACTGGGAAGCGCCTCTCCAGGGGCTg < 1:564765/140‑1 (MQ=255) aTCTGGTTAGCGTCGGCGCGTATGCCAAAGGCAGCGATCCGATGCTCGATAAAGCCATCGCCCTGTGGCCGCAGCTGGAGGGCTATTTGCAACAAGGCATTTTTGAACGCGCGGACTGGGAAGCGCCTCTCCAGGGGCTg > 1:62108/1‑140 (MQ=255) cTGGTTAGCGTCGGCGCGTATGCCAAAGGCAGCGATCCGATGCTCGATAAAGCCATCGCCCTGTGGCCGCAGCTGGAGGGCTATTTGCAACAAGGCATTTTTGAACGCGCGGACTGGGAAGCGCCTCTCCAGGGGCTGGa < 1:793516/140‑1 (MQ=255) ttAGCGTCGGCGCGTCTGCCAACGGCAGCTATCCGATTCTCGATAAAGCCATCGCCCTGTGGCCGCAGCTGGAGGGCTATTTGCAACAAGGCATTTTTGAACGCGCGGACTGGGAAGCGCCTCTCCAGGGGCTGGAGCGt < 1:324719/140‑1 (MQ=255) ttAGCGTCCGCGCGTATGCCAATGGCAGCGACCAGCTGCCCGATAAAGCCATCGCCCTGTGGCCGCAGCTGGAGGGCTATTTGCAACAAGGCATTTTTGAACGCGCGGACTGGGAAGCGCCTCTCCAGGGGCTGGAGCGt < 2:423032/140‑1 (MQ=255) cGTCGGCGCGTATGCCAAAGGCAGCGATCCGATGCTCGATAAAGCCATCGCCCTGTGGCCGCAGGTGGAGGGCTATTTGCAACAAGGCATTTTTGAACGCGCGGACTGGGAAGCGCCTCTCCAGGGGGTGGAGCGTActt > 2:773413/1‑140 (MQ=255) cGTCGGCGCGTATGCCAAAGGCAGCGATCCGATGCTCGATAAAGCCATCGCCCTGTGGCCGCAGCTGGAGGGCTATTTGCAACAAGGCATTTTTGAACGCGCGGACTGGGAAGCGCCTCTCCAGGGGCTGGAGCGTAttt > 1:866326/1‑140 (MQ=255) cGGCGCGTATGCCAAAGGCAGCGATCCGATGCTCGATAAAGCCATCGCCCTGTGGCCGCAGCTGGAGGGCTATTTGCAACAAGGCATTTTTGAACGCGCGGACTGGGAAGCGCCTCTCCAGGGGCTGGAGCGTATTTTcc < 1:520390/140‑1 (MQ=255) gcgcGTATGCCAAAGGCAGCGATCCGATGCTCGATAAAGCCATCGCCCTGTGGCCGCAGCTGGAGGGCTATTTGCAACAAGGCATTTTTGAACGCGCGGACTTGGAAGCGACTCGCCGGGGGCTGGAGCGTATTTTCCCg > 1:278224/1‑140 (MQ=255) gcgcGTATGCCAAAGGCAGCGATCCGATGCTCGATAAAGCCATCGCCCTGTGGCCGCAGCTGGAGGGCTATTTGCAACAAGGAATTTTTGAACGCGCGGACTGGGAAGCGCCTCTCCAGGGGCTGGAGCGTATTTTCCCg > 2:531187/1‑140 (MQ=255) cgcgTATGCCAAAGGCAGCGATCCGATGCTCGATAAAGCCATCGCCCTGTGGCCGCAGCTGGAGGGCTATTTGCAACAAGGCATTTTTGAACGCGCGGACTGGGAAGCGCCTCTCCAGGGGCTGGAGCGTATTTTCCCGa < 1:624865/140‑1 (MQ=255) cgTATGCCAAAGGCAGCGATCCGATGCTCGATAAAGCCATCGCCCTGTGGCCGCAGCTGGAGGGCTATTTGCAACAAGGCATTTTTGAACGCGCGGACTGGGAAGCGCCTCTCCAGGGGCTGGAGCGTATTTTCCCGACa < 1:531187/140‑1 (MQ=255) cgTATGCCAAAGGCAGCGATCCGATGCTCGATAAAGCCATCGCCCTGTGGCCGCAGCTGGAGGGCTATTTGCAACAAGGCATTTTTGAACGCGCGGACTGGGAAGCGCCTCTCCAGGGGCTGGAGCGTATTTTCCCGACa < 1:667735/140‑1 (MQ=255) cgTATGCCAAAGGCAGCGATCCGATGCTCGATAAAGCCATCGCCCTGTGGCCGCAGCTGGAGGGCTATTTGCAACAAGGCATTTTTGAACGCGCGGACTGGGAAGCGCCTCTCCAGGGGCTGGAGCGTATTTTCCCGACa < 1:692288/140‑1 (MQ=255) cGATCCGATGCTCGATAAAGCCATCGCCCTGTGGCCGCAGCTGGAGGGCTATTTGCAACAAGGCATTTTTGAACGCGCGGACTGGGAAGCGCCTCTACAGGGGCTGGAGCGTATTTTCCCGACAg > 2:592852/1‑125 (MQ=255) cGATCCGATGCTCGATAAAGCCATCGCCCTGTGGCCGCAGCTGGAGGGCTATTTGCAACAAGGCATTTTTGAACGCGCGGACTGGGAAGCGCCTCTACAGGGGCTGGAGCGTATTTTCCCGACAg < 1:592852/125‑1 (MQ=255) cTCGATAAAGCCATCGCCCTGTGGCCGCAGCTGGAGGGCTATTTGCAACAAGGCATTTTTGAACGCGCGGACTGGGAAGCGCCTCTCCAGGGGCTGGAGCGTATTTTCCCGACAGTGTCATAACCCAGGAGATAACGGCa < 2:614283/140‑1 (MQ=255) cTCGATAAAGCCATCGCCCTGTGGCCGCAGCTGGAGGGCTATTTGCAACAAGGCATTTTTGAACGCGCGGACTGGGAAGCGCCTCTCCAGGGGCTGGAGCGTATTTTCCCGACAGTGTCATAACCCAGGAGATAACGGCa < 2:866326/140‑1 (MQ=255) gATAAAGCCATCGCCCTGTGGCCGCAGCTGGAGGGCTATTTGCAACAAGGCATTTTTGAACGCGCGGACTGGGAAGCGCCTCTCCAGGGGCTGGAGCGTATTTTCCCGACAGTGTCATAACCCAGGAGATAACGGCAGAt < 1:785329/140‑1 (MQ=255) aGCCATCGCCCTGTGGCCGCAGCTGGAGGGCTATTTGCAACAAGGCATTTTTGAACGCGCGGACTGGGAAGCGCCTCTCCAGGGGCTGGAGCGTATTTTCCCGACAGTGTCATAACCCAGGAGATAACGGCAGATGGCAg > 2:486184/1‑140 (MQ=255) gCCATCGCCCTGTGGTCGCAGCTGGAGGGCTATTTGCAACAAGGCATTTTTGAACGCGCGGACTGGGAAGCGCCTCTCCAGGGGCTGGAGCGTATTTTCCCGACAGTGTCATAACCCAGGAGATAACGGCAGATGGCAGa < 2:577084/140‑1 (MQ=255) tgtgGCCGCAGCTGGAGGGCTATTTGCAACAAGGCATTTTTGAACGCGCGGACTGGGAAGCGCCTCTCCAGGGGCTGGAGCGTATTTTCCCGACAGTGTCATGACCCAGGGGATAACTGCAGATTGCAGAACATGGTgcg > 1:246268/1‑140 (MQ=255) tgtgGCCGCAGCTGGAGGGCTATTTGCAACAAGGCATTTTTGAACGCGCGGACTGGGAAGCGCCTCTCCAGGGGCTGGAGCGTATTTTCCCGACAGTGTCATAACCCAGGAGATAACGGCAGATGGCAGAACATGGTgcg > 1:627890/1‑140 (MQ=255) gtgGCCGCAGCTGGAGGGCTATTTGCAACAAGGCATTTTTGAACGCGCGGACTGGGAAGCGCCTCTCCAGGGGCTGGAGCGTATTTTCCCGACAGTGTCATAACCCAGGAGATAACGGCAGATg < 1:151709/124‑1 (MQ=255) gtgGCCGCAGCTGGAGGGCTATTTGCAACAAGGCATTTTTGAACGCGCGGACTGGGAAGCGCCTCTCCAGGGGCTGGAGCGTATTTTCCCGACAGTGTCATAACCCAGGAGATAACGGCAGATg > 2:151709/1‑124 (MQ=255) gtgGCCGCAGCTGGAGGGCTATTTGCAACAAGGCATTTTTGAACGCGCGGACTGGGAAGCGCCTCTCCAGGGGCTGGAGCGTATTTTCCCGACAGTGTCATAACCCAGGAGATAACGGCAGATGGCAGAACATGGTgcgc > 2:684906/1‑140 (MQ=255) ccGCAGCTGGAGGGCTATTTGCTACAAGGCATTTTTGAAGGCGCGGACTGGGAAGCGCCTCTCCAGGGGCTGGAGCGTATTTTCCCGACAGTGTCATAACCCAGGAGATAACGGCAGATGGCAGAACATGGTGCGCTGGc < 2:401771/140‑1 (MQ=255) gCAGCTGGAGGGCTATTAGCAACAAGGCATTCTTGTACGGGCGGACTGTGAAGCGCCTCTCCAGGGGCTGGAGCGTATTTTCCCGACAGTGTCATAACCCAGGAGATAACGGCAGATGGCAGAACATGGTGCGCTGGCGa < 2:278224/140‑1 (MQ=255) cAGCTGGAGGGCTATTTGCAACAAGGCATTTTTGAACGCGCGGACTGGGAAGCGCCTCTCCAGGGGCTGGAGCGTATTTTCCCGACAGTGTCATAACCCAGGAGATAACGGCAGATGGCAGAACATGGTGCGCTGGCGAc > 1:552703/1‑140 (MQ=255) tttGCAACAAGGCATTTTTGAACGCGCGGACTGGGAAGCGCCTCTCCAGGGGCTGGAGCGTATTTTCCCGACAGTGTCATAACCCAGGAGATAACGGCAGATGGCAGAACATGGTGCGCTGGCGACCCTGAAAGATCTgg > 1:89287/1‑140 (MQ=255) acaaGGCATTTTTGAACCCGCGCACTGGGAATCGCGTCTTCAGGGGCTTGAGCGTATTTTCCCGACAGTGTCATAACCCAGGAGATAACGGCAGATGGCAGAACATGGTGCGCTGGCGACCCTGAAAGATCTGGCAGaaa < 2:246268/140‑1 (MQ=255) aaGGCATTTTTGAACGCGCGGACTGGGAAGCGCCTCTCCAGGGGCTGGAGCGTATTTTCCCGACAGTGTCATAACCCAGGAGATAACGGCAGATGGCAGAACATGGTGCGCTGGCGACCCTGAAAGATCTGGCAGaaaaa < 1:773413/140‑1 (MQ=255) ggCATTTTTGAACGCGCGGACTGGGAAGCGCCTCTCCAGGGGCTGGAGCGTATTTTCCCGACAGTGTCATAACCCAGGAGATAACGGCAGATGGCAGAACATTGTGCGCTGGCGACCGTGAAAGTTCTGGCAGAAAAaga > 1:291769/1‑140 (MQ=255) ggACTGGGAAGCGCCTCTCCAGGGGCTGGAGCGTATTTTCCCGACAGTGTCATAACCCAGGAGATAACGGCAGATGGCAGAACATGGTGCGCTGGCGACCCTGAAAGATCTGGCAGAAAAAGAGGTAGAGGATGCcgcgc < 1:600078/140‑1 (MQ=255) | TAACCGCGATCTGGTTAGCGTCGGCGCGTATGCCAAAGGCAGCGATCCGATGCTCGATAAAGCCATCGCCCTGTGGCCGCAGCTGGAGGGCTATTTGCAACAAGGCATTTTTGAACGCGCGGACTGGGAAGCGTCTCTCCAGGGGCTGGAGCGTATTTTCCCGACAGTGTCATAACCCAGGAGATAACGGCAGATGGCAGAACATGGTGCGCTGGCGACCCTGAAAGATCTGGCAGAAAAAGAGGTAGAGGATGCCGCGC > NC_000913/2017753‑2018012 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |