Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	240,779	G→A	S146N (AGC→AAC)	ivy →	periplasmic chaperone, inhibitor of vertebrate C‑type lysozyme

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	240,779	0	G	A	100.0%	99.9 / NA	31	S146N (AGC→AAC)	ivy	periplasmic chaperone, inhibitor of vertebrate C‑type lysozyme
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (17/14);  total (17/14)

GATGACGGGGCTGTTCTCGACTATTGATGAGAAAACGTCGCAAGAGAAACTCACCTGGCTGAATGTGAACGATGCGCTTTCGATTGATGGTAAAACGGTGTTGTTCGCGGCGTTGACCGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCT  >  NC_000913/240657‑240905                                                                                                                           |                                                                                                                               gATGACGGGGCTGTTCTCGACTATTGATGAGAAAACGTCGCAAGAGAAACTCACCTGGCTGAATGTGAACGATGCGCTTTCGATTGATGGTAAAACGGTGTTGTTCGCGGCGTTGACCGGCAACCTGGAAAACCATCCgg                                                                                                               >  1:675892/1‑140 (MQ=255)  aTGACGGGGCTGTTCTCGACTATTGATGAGAAAACGTCGCAAGAGAAACTCACCTGGCTGAATGTGAACGATGCGCTTTCGATTGATGGTAAAACGGTGTTGTTCGCGGCGTTGCCCGGCAACCGGGAAAACCATCCGGa                                                                                                              >  1:358230/1‑140 (MQ=255)  aTGACGGGGCTGTTCTCGACTATTGATGAGAAAACGTCGCAAGAGAAACTCACCTGGCTGAATGTGAACGATGCGCTTTCGATTGATGGTAAAACGGTGTTGTTCGCGGCGTTGACCGGCAACCTGGAAAACCATCCGGa                                                                                                              >  1:65993/1‑140 (MQ=255)          gCTGTTCTCGACTATTGATGAGAAAACGTCGCAAGAGAAACTCACCTGGCTGAATGTGAACGATGCGCTTTCGATTGATGGTAAAACGGTGTTGTTCGCGGCGTTGACCGGCAACCTGGAAAACCATCCGGATGGCTTTa                                                                                                      <  2:803093/140‑1 (MQ=255)            tGTTCTCGACTATTGATGAGAAAACGTCGCAAGAGAAACTCACCTGGCTGAATGTGAACGATGCGCTTTCGATTGATGGTAAAACGGTGTTGTTCGCGGCGTTGACCGGCAACCTGGAAAACCATCCGGa                                                                                                              <  2:94979/130‑1 (MQ=255)            tGTTCTCGACTATTGATGAGAAAACGTCGCAAGAGAAACTCACCTGGCTGAATGTGAACGATGCGCTTTCGATTGATGGTAAAACGGTGTTGTTCGCGGCGTTGACCGGCAACCTGGAAAACCATCCGGa                                                                                                              >  1:94979/1‑130 (MQ=255)            tGTTCTCGACTATTGATGAGAAAACGTCGCAAGAGAAACTCACCTGGCTGAATGTGAACGATGCGCTTTCGATTGATGGTAAAACGGTGTTGTTCGCGGCGTTGACCGGCAACCTGGAAAACCATCCGGATGGCTTTAAt                                                                                                    >  2:51987/1‑140 (MQ=255)                          gatgaGAAAACGTCGCAAGAGAAACTCACCTGGCTGAATGTGAACGATGCGCTTTCGATTGATGGTAAAACGGTGTTGTTCGCGGCGTTGACCGGCAACCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGc                                                                                      <  1:514202/140‑1 (MQ=255)                             gagaAAACGTCGCAAGAGAAACTCACCTGGCTGAATGTGAACGATGCGCTTTCGATTGATGGTAAAACGGTGTTGTTCGCGGCGTTGACCGGCAACCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGa                                                                                   <  2:536489/140‑1 (MQ=255)                                        gCAAGAGAAACTCACCTTGCTGTATGTGAACTATGCGCTCTCGATTGAGGGTAAAACGGTGTTGTTCGCGGCGTTGACCGGCAACCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACgg                                                                        <  2:305046/140‑1 (MQ=255)                                        gCAAGAGAAACTCACCTGGCTGAATGTGAACGATGCGCTTTCGATTGATGGTAAAACGGTGTTGTTCGCGGCGTTGACCGGCAACCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGa                                                                                   <  1:474845/129‑1 (MQ=255)                                        gCAAGAGAAACTCACCTGGCTGAATGTGAACGATGCGCTTTCGATTGATGGTAAAACGGTGTTGTTCGCGGCGTTGACCGGCAACCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGa                                                                                   >  2:474845/1‑129 (MQ=255)                                                 aCTCACCTGGCTGAATGTGAACGATGCGCTTTCGCTTGATGGTAAAACGGTGTTGTTCGCGGCGTTGACCGGCAACCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATGAAGAAACGGAGCCTTTCg                                                               >  2:170513/1‑140 (MQ=255)                                                  cTCACCTGGCTGAATGTGAACGATGCGCTTTCGATTGATGGTAAAACGGTGTTGTTCGCGGCGTTGACCGGCAACCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCgg                                                              <  2:65993/140‑1 (MQ=255)                                                   tCACCTGGCTGAATGTGAACGATGCGCTTTCGATTGATGGTAAAACGGTGTTGTTCGCGGCGTTGACCGGCAACCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGc                                                             <  1:51987/140‑1 (MQ=255)                                                    cACCTGGCTGAATGTCAACGATCCGATTTAGATTGATGGTAAAACGGTGTTGTTCGCGGCGTTGACCGGCAACCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCt                                                            <  1:367697/140‑1 (MQ=255)                                                            tGAATGTGAACGATGCGCTTTCGATTGATGGTAAAACGGTGTTGTTCGCGGCGTTGACCGGCAACCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTAtt                                                    >  2:708960/1‑140 (MQ=255)                                                               aTGTGAACGATGCGCTTTCGATTGATGGTAAAACGGTGTTGTTCGCGGCGTTGACCGGCAACCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCAt                                                 >  2:12252/1‑140 (MQ=255)                                                                    aaCGATGCGCTTTCGATTGATGGTAAAACGGTGTTGTTCGCGGCGTTGACCGGCAACCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCt                                                            <  1:810626/124‑1 (MQ=255)                                                                    aaCGATGCGCTTTCGATTGATGGTAAAACGGTGTTGTTCGCGGCGTTGACCGGCAACCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCt                                                            >  2:810626/1‑124 (MQ=255)                                                                        aTGCGCTTTCGATTGATGGTAAAACGGTGTTGTTCGCGGCGTTGACCGGCAACCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTGGCGGATAAAGAAACGGAGCCTTTCGGCTCCTATATTCATCTACGCGGc                                        >  2:409517/1‑140 (MQ=255)                                                                        aTGCGCTTTCGATTGATGGTAAAACGGTGTTGTTCGCGGCGTTGACCGGCAACCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGc                                        >  1:626907/1‑140 (MQ=255)                                                                          gcgcTTTCGATTGATGGTAAAACGGTGTTGTTCGCGGCGTTGACCGGCAACCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTAcg                                            <  2:592220/134‑1 (MQ=255)                                                                          gcgcTTTCGATTGATGGTAAAACGGTGTTGTTCGCGGCGTTGACCGGCAACCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTAcg                                            >  1:592220/1‑134 (MQ=255)                                                                           cgcTTTCGATTGATGGTAAAACGGTGTTGTTCGCGGCGTTGACCGGCAACCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTc                                     >  1:683798/1‑140 (MQ=255)                                                                            gcTTTCGATTGATGGTAAAACGGTGTTGTTCCCGGCGTTTACCGGCAATCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCa                                    <  2:358230/140‑1 (MQ=255)                                                                                  gATTGATGGTAAAACGGTGTTGTTCGCGGCGTTGACCGGCAACCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTc                              <  1:562598/140‑1 (MQ=255)                                                                                     tGATGGTAAAACGGTGTTGTTCGCGGCGTTGACCGGCAACCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGc                           >  2:470628/1‑140 (MQ=255)                                                                                      gATGGTAAAACGGTGTTGTTCGCGGCGTTGACCGGCAACCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCa                          >  2:138367/1‑140 (MQ=255)                                                                                                   tgttgtTCGCGGCGTTGACCGGCAACCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCaa             >  1:525937/1‑140 (MQ=255)                                                                                                              gcgTTGACCGGCAACCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCt  <  2:683798/140‑1 (MQ=255)                                                                                                                           |                                                                                                                               GATGACGGGGCTGTTCTCGACTATTGATGAGAAAACGTCGCAAGAGAAACTCACCTGGCTGAATGTGAACGATGCGCTTTCGATTGATGGTAAAACGGTGTTGTTCGCGGCGTTGACCGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCT  >  NC_000913/240657‑240905

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑