Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,024,436	C→T	Q157* (CAA→TAA)	rcsA →	DNA‑binding transcriptional activator RcsA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,024,436	0	C	T	100.0%	250.2 / NA	71	Q157* (CAA→TAA)	rcsA	DNA‑binding transcriptional activator RcsA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (33/38);  total (33/38)

TCGACGATATCCTTGGCGATATTCTGAAAAAAGAGACAACGATAACCTCGTTTTTAAATATGCCGACGTTATCATTGAGCCGAACCGAATCGAGTATGTTGCGAATGTGGATGGCAGGTCAGGGAACCATTCAAATCTCTGACCAAATGAATATCAAAGCCAAGACCGTTTCATCGCATAAAGGTAATATTAAACGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCGTCCGACTGACGGATAATGTG  >  NC_000913/2024305‑2024561                                                                                                                                    |                                                                                                                              tcGACGATATCCTTGGCGATATTCTGAAAAAAGAGACAACGATAACCTCGTTTTTAAATATGCCGACGTTATCATTGAGCCGAACCGAATCGAGTATGTTTCGAATGTAGATGGCAGATCAGGGAACCATTTAAATctct                                                                                                                       >  2:362402/1‑140 (MQ=255)    acgaTATCCTTGGCGATATTCTGAAAAAAGAGACAACGATAACCTCGTTTTTAAATATGCCGACGTTATCATTGAGCCGAACCGAATCGAGTATGTTGCGAATGTGGATGGCAGGTCAGGGAACCATTTAAATCTCTGAc                                                                                                                    <  1:101109/140‑1 (MQ=255)    acgaTATCCTTGGCGATATTCTGAAAAAAGAGACAACGATAACCTCGTTTTTAAATATGCCGACGTTATCATTGAGCCGAACCGAATCGAGTATGTTGCGAAGGTGGATGGCAGTTCCGGGAACCATTTAAATCTCTGAc                                                                                                                    >  1:320049/1‑140 (MQ=255)     cgaTATCCTTGGCGATATTCTGAAAAAAGAGACAACGATAACCTCGTTTTTAAATATGCCGACGTTATCATTGAGCCGAACCGAATCGAGTATGTTGCGAATGTGGATGGCAGGTCAGGGAACCATTTAAATCTCTGAcc                                                                                                                   <  1:600872/140‑1 (MQ=255)     cgaTATCCTTGGCGATATTCTGAAAAAAGAGACAACGATAACCTCGTTTTTAAATATGCCGACGTTATCATTGAGCCGAACCGAATCGAGTATGTTGCGAATGTGGATGGCAGGTCAGGGAACCATTTAAATCTCTGAcc                                                                                                                   <  2:484455/140‑1 (MQ=255)               ggCGATATTCTGAAAAAAGAGACAACGATAACCTCGTTTTTAAATATGCCGACGTTATCATTGAGCCGAACCGAATCGAGTATGTTGCGAATGTGGATGGCAGGTCAGGGAACCATTTAAATCTCTGACCAAATGAatat                                                                                                         >  1:786262/1‑140 (MQ=255)               ggCGATATTCTGAAAAAAGAGACAACGATAACCTCGTTTTTAAATATGCCGACGTTATCATTGAGCCGAACCGAATCGAGTATGTTGCGAATGTGGATGGCAGGTCAGGGAACCATTTAAATCTCTGACCAAATGAatat                                                                                                         >  2:747923/1‑140 (MQ=255)               ggCGATATTCTGAAAAAAGAGACAACGATAACCTCGTTTTTAAATATGCCGACGTTATCATTGAGCCGAACCGAATCGAGTATGTTGCGAATGTGGATGGCAGGTCAGGGAACCATTTAAATCTCTGACCAAATGAatat                                                                                                         >  1:140199/1‑140 (MQ=255)               ggCGATATTCTGAAAAAAGAGACAACGATAACCTCGTTTTTAAATATACCGACGTTATCATTGAGCCGAACCGAATCGAGTATGTTGCGAATGTGGATGGCAGGTCAGGGAACCATTTAAATCTCTGACCAAATGAatat                                                                                                         >  2:131712/1‑140 (MQ=255)                gCGATATTCTGAAAAAAGAGACAACGATAACCTCGTTTTTAAATATGCCGACGTTATCATTGAGCCGAACCGAATCGAGTATGTTGCGAATGTGGATGGCAGGTCAGGGAACCATTTAAATCTCTGACCAAATGAATATc                                                                                                        <  2:613000/140‑1 (MQ=255)                    tatTCTGAAAAAAGAGACAACGATAACCTCGTTTTTAAATATGCCGACGTTATCATTGAGCCGAACCGAATCGAGTATGTTGCGAATGTGGATGGCAGGTCAGGGAACCATTTAAATCTCTGACCAAATGAATATCAAAg                                                                                                    >  2:832104/1‑140 (MQ=255)                      ttCTGAAATAAGAGACATCGATTACCTCGTGTTAAAATACGCCGACGTTATCATTGAGCCGAACCGAATCGAGTATGTTGCGAATGTGGATGGCAGGTCAGGGAACCATTTAAATCTCTGACCAAATGAATATCAAAGcc                                                                                                  <  2:414684/140‑1 (MQ=255)                      ttCTGAAAAAAGAGACAACGATAACCTCGTTTTTAAATATGCCGACGTTATCATTGAGCCGAACCGAATCGAGTATGTTGCGAATGTGGATGGCAGGTCAGGGAACCATTTAAATCTCTGACCAAATGAATATCAAAGcc                                                                                                  >  2:542258/1‑140 (MQ=255)                        cTGAAAAAAGAGACAACGATAACCTCGTTTTTAAATATGCCGACGTTATCATTGAGCCGAACCGAATCGAGTATGTTGCGAATGTGGATGGCAGGTCAGGGAACCATTTAAATCTCTGACCAAATGAATATCAAAGCCaa                                                                                                <  1:832104/140‑1 (MQ=255)                          gAAAAAAGAGACAACGATAACCTCGTTTTTAAATATGCCGACGTTATCATTGAGCCGAACCGAATCGAGTATGTTGCGAATGTGGATGGCAGGTCAGGGAACCATTTAAATCTCTGACCAAATGAATATCAAAGCCAAGa                                                                                              <  1:624081/140‑1 (MQ=255)                          gAAAAAAGAGACAACGACAACCCCGTTTTTACATACGCCCACGTTATCTTTGAGCCGAACCGAATCGAGTATGTTGCGAATGTGGATGGCAGGTCAGGGAACCATTTAAATCTCTGACCAAATGAATATCAAAGCCAAGa                                                                                              <  2:237218/140‑1 (MQ=255)                                agagACAACGATAACCTCGTTTTTAAATATGCCGACGTTATCATTGAGCCGAACCGAATCGAGTATGTTGCGAATGTGGATGGCAGGTCAGGGAACCATTTAAATCTCTGACCAAATGAATATCAAAGCCAAGACCGttt                                                                                        >  2:759275/1‑140 (MQ=255)                                   gaCAACGATAACCTCGTTTTTAAATATGCCGACGTTATCATTGAGCCGAACCGAATCGAGTATGTTGCGAATGTGGATGGCAGGTCAGGGAACCATTTAAATCTCTGACCAAATGAATATCAAAGCCAAGACCGTTTCAt                                                                                     >  1:820984/1‑140 (MQ=255)                                     cAACGATAACCTCGTTTTTAAATATGCCGACGTTATCATTGAGCCGAACCGAATCGAGTATGTTGCGAATGTGGATGGCAGGTCAGGGAACCATTTAAATCTCTGACCAAATGAATATCAAAGCCAAGACCGTTTCATCg                                                                                   >  1:528800/1‑140 (MQ=255)                                        cGATAACCTCGTTTTTAAATATGCCGACGTTATCATTGAGCCGAACCGAATCGAGTATGTTGCGAATGTGGATGGCAGGTCAGGGAACCATTTAAATCTCTGACCAAATGAATATCAAAGCCAAGACCGTTTCATCGCAt                                                                                <  2:139638/140‑1 (MQ=255)                                        cGATAACCTCGTTTTTAAATATGCCGACGTTATCATTGAGCCGAACCGAATCGAGTATGTTGCGAATGTGGATGGCAGGTCAGGGAACCATTTAAATCTCTGACCAAATGAATATCAAAGCCAAGACCGTTTCATCGCAt                                                                                <  1:745055/140‑1 (MQ=255)                                        cGATAACCTCGTTTTTAAATATGCCGACGTTATCATTGAGCCGAACCGAATCGAGTATGTTGCGAATGTGGATGGCAGGTCAGGGAACCATTTAAATCTCTGACCAAATGAATATCAAAGCCAAGACCGTTTCATCGCAt                                                                                <  1:759275/140‑1 (MQ=255)                                         gATAACCTCGTTTTTAAATATGCCGACGTTATCATTGAGCCGAACCGAATCGAGTATGTTGCGAATGTGGATGGCAGGTCAGGGAACCATTTAAATCTCTGACCAAATGAATATCAAAGCCAAGACCGTTTCAt                                                                                     <  1:43865/134‑1 (MQ=255)                                         gATAACCTCGTTTTTAAATATGCCGACGTTATCATTGAGCCGAACCGAATCGAGTATGTTGCGAATGTGGATGGCAGGTCAGGGAACCATTTAAATCTCTGACCAAATGAATATCAAAGCCAAGACCGTTTCAt                                                                                     >  2:43865/1‑134 (MQ=255)                                         gATAACCTCGTTTTTAAATATGCCGACGTTATCATTGAGCCGAACCGAATCGAGTATGTTGCGAATGTGGATGGCAGGTCAGGGAACCATTTAAATCTCTGACCAAATGAATATCAAAGCCAAGACCGTTTCATCGCATa                                                                               <  2:786262/140‑1 (MQ=255)                                                  gTTTTTAAATATGCCGACGTTATCATTGAGCCGAACCGAATCGAGTATGTTGCGAATGTGGATGGCAGGTCAGGGAACCATTTAAATCTCTTACCAAATGCATAACAAAGCCAAGACCGTTTTATCGCATAAAGGTAata                                                                      >  2:344383/1‑140 (MQ=255)                                                  gTTTTTAAATATGCCGACGTTATCATTGAGCCGAACCGAATCGAGTATGTTGCGAATGTGGATGGCAGGTCAGGGAACCATTTAAATCTCTGACCAAATGAATATCAAAGCCAAGACCGTTTCATCGCATAAAGGTAata                                                                      >  1:864171/1‑140 (MQ=255)                                                  gTTTTTAAATATGCCGACCTTATCATTGAGCCGAACCGAATCGAGTATGTTGCGAATGTGGATGGCAGGTCAGGGAACCATTTAAATCTCTGACCAAATGAATATCAAAGCCAAGACCGTTTCATCGCATAAAGGTAata                                                                      >  2:87783/1‑140 (MQ=255)                                                   tttttAAATATGCCGACGTTATCATTGAGCCGAACCGAATCGAGTATGTTGCGAATGTGGATGGCAGGTCAGGGAACCATTTAAATCTCTGACCAAATGAATATCAAAGCCAAGACCGTTTCATCGCATAAAGGTAatat                                                                     >  2:848996/1‑140 (MQ=255)                                                   tttttAAATATGCCGACGTTATCATTGAGCCGAACCGAATCGAGTATGTTGCGAATGTGGATGGCAGGTCAGGGAACCATTTAAATCTCTGACCAAATGAATATCAAAGCCAAGACCGTTTCATCGCATAAAGGTAatat                                                                     <  2:528800/140‑1 (MQ=255)                                                   tttttAAATATGCCGACGTTATCATTGAGCCGAACCGAATCGAGTATGTTGCGAATGTGGATGGCAGGTCAGGGAACCATTTAAATCTCTGACCAAATGAATATCAAAGCCAAGACCGTTTCATCGCATAAAGGTAatat                                                                     <  2:140199/140‑1 (MQ=255)                                                         aaTATGCCGACGTTATCATTGAGCCGAACCGAATCGAGTATGTTGCGAATGTGGATGGCAGGTCAGGGAACCATTTAAATCTCTGACCAAATGAATATCAAAGCCAAGACCGTTTCATCGCATAAAGGTAATATTAAACg                                                               <  1:540483/140‑1 (MQ=255)                                                              gCCGACGTTATCATTGAGCCGAACCGAATCGAGTATGTTGCGAATGTGGATGGCAGGTCAGGGAACCATTTAAATCTCTGACCAAATGAATATCAAAGCCAAGACCGTTTCATCGCATAAAGGTAATATTAAACGTAAGa                                                          >  2:727734/1‑140 (MQ=255)                                                              gCCGACGTTATCATTGAGCCGAACCGAATCGAGTATGTTGCGAATGTGGATGGCAGGTCAGGGAACCATTTAAATCTCTGACCAAATGAATATCAAAGCCAAGACCGTTTCATCGCATAAAGGTAATATTAAAAGTAAGa                                                          >  1:746723/1‑140 (MQ=255)                                                                   cGTTATCATTGAGCCGAACCGAATCGAGTATGTTGCGAATGTGGATGGCAGGTCAGGGAACCATTTAAATCTCTGACCAAATGAATATCAAAGCCAAGACCGTTTCATCGCATAAAGGCAATATTAAACGTAAGATCaaa                                                     >  2:456317/1‑140 (MQ=255)                                                                      tATCATTGAGCCGAACCGAATCGAGTATGTTGCGAATGTGGATGGCAGGTCAGGGAACCATTTAAATCTCTGACCAAATGAATATCAAAGCCAAGACCGTTTCATCGCATAAAGGTAATATTAAACGTAAGATCAAAACg                                                  <  1:848996/140‑1 (MQ=255)                                                                        tCATTGAGCCGAACCGAATCGATTAGGTTACGAATGTGGATGGCAGGTCAGGGAACCATTTAAATCTCTGACCAAATGAATATCAAAGCCAAGACCGTTTCATCGCATAAAGGTAATATTAAACGTAAGATCAAAACGCa                                                <  1:344383/140‑1 (MQ=255)                                                                        tCATTGAGCCGAACCGAATCGAGTATGTTGCGAATGTGGATGGCAGGTCAGGGAACCATTTAAATCTCTGACCAAATGAATATCAAAGCCAAGACCGTTTCATCGCATAAAGGTAATATTAAACGTAAGATCAAAACGCa                                                <  2:119371/140‑1 (MQ=255)                                                                        tCATTGAGCCGAACCGAATCGAGTATGTTGCGAATGTGGATGGCAGGTCAGGGAACCATTTAAATCTCTGACCAAATGAATATCAAAGCCAAGACCGTTTCATCGCATAAAGGTAATATTAAACGTAAGATCAAAACGCa                                                <  1:87783/140‑1 (MQ=255)                                                                             gAGCCGAACCGAATCGAGTATGTTGCGAATGTGGATGGCAGGTCAGGGAACCATTTAAATCTCTGACCAAATGAATATCAAAGCCAAGACCGTTTCATCGCATAAAGGTAATATTAAACGTAAGATCAAAACGCataata                                           >  2:558109/1‑140 (MQ=255)                                                                             gAGCCGAACCGAATCGAGTATGTTGCGAATGTGGATGGCAGGTCAGGGAACCATTTAAATCTCTGACCAAATGAATATCAAAGCCAAGACCGTTTCATCGCATAAAGGTAATATTAAACGTAAGATCAAAACGCataata                                           <  1:727734/140‑1 (MQ=255)                                                                                ccgaaccgaaTCGAGCATGTTGCGACTGTGGATGGCAGGTCAGGGAACCATTTAAATCTCTGACCAAATGAATATCAAAGCCAAGACCGTTTCATCGCATAAAGGTAATATTAAACGTAAGATCAAAACGCATAATAAAc                                        <  1:362402/140‑1 (MQ=255)                                                                                   aaccgaaTCGAGTATGTTGCGAATGTGGATGGCAGGTCAGGGAACCATTTAAATCTCTGACCAAATGAATATCAAAGCCAAGACCGTTTCATCGCATAAAGGTAATATTAAACGTAAGATCAAAACGCATAATAAAc                                        <  1:43692/137‑1 (MQ=255)                                                                                   aaccgaaTCGAGTATGTTGCGAATGTGGATGGCAGGTCAGGGAACCATTTAAATCTCTGACCAAATGAATATCAAAGCCAAGACCGTTTCATCGCATAAAGGTAATATTAAACGTAAGATCAAAACGCATAATAAAc                                        >  2:43692/1‑137 (MQ=255)                                                                                   aaccgaaTCGAGTATGTTGCGAATGTGGATGGCAGGTCAGGGAACCATTTAAATCTCTGACCAAATGAATATCAAAGCCAAGACCGTTTCATCGCATAAAGGTAATATTAAACGTAAGATCAAAACGCATAATAAACAgg                                     <  1:686218/140‑1 (MQ=255)                                                                                   aaccgaaTCGAGTATGTTGCGAATGTGGATGGCAGGTCAGGGAACCATTTAAATCTCTGACCAAATGAATATCAAAGCCAAGACCGTTTCATCGCATAAAGGTAATATTAAACGTAAGATCAAAACGCATAATAAACAgg                                     >  1:676282/1‑140 (MQ=255)                                                                                   aaccgaaTCGAGTATGTTGCGAATGTGGATGGCAGGTCAGGGAACCATTTAAATCTCTGACCAAATGAATATCAAAGCCAAGACCGTTTCATCGCATAAAGGTAATATTAAACGTAAGATCAAAACGCATAATAAACAgg                                     >  2:840549/1‑140 (MQ=255)                                                                                     ccgaaTCGAGTATGTTGCGAATGTGGATGGCAGGTCAGGGAACCATTTAAATCTCTGACCAAATGAATATCAAAGCCAAGACCGTTTCATCGCATAAAGGTAATATTAAACGTAAGATCAAAACGCATAATAAACAGGtt                                   <  2:820984/140‑1 (MQ=255)                                                                                     ccgaaTCGAGTATGTTGCGAATGTGGATGGCAGGTCAGGGAACCATTTAAATCTCTGACCAAATGAATATCAAAGCCAAGACCGTTTCATCGCATAAAGGTAATATTAAACGTAAGATCAAAACGCATAATAAACAGGtt                                   <  1:747923/140‑1 (MQ=255)                                                                                     ccgaaTCGAGTATGTTGCGAATGTGGATGGCAGGTCAGGGAACCATTTAAATCTCTGACCAAATGAATATCAAAGCCAAGACCGTTTCATCGCATAAAGGTAATATTAAACGTAAGATCAAAACGCATAATAAACAGGtt                                   <  1:542258/140‑1 (MQ=255)                                                                                        aaTCGAGTATGTTGCGAATGTGGATGGCAGGTCAGGGAACCATTTAAATCTCTGACCAAATGAATATCAAAGCCAAGACCGTTTCATCGCATAAAGGTAATATTAAACGTAAGATCAAAACGCATAATAAACAGGTTATc                                >  1:546012/1‑140 (MQ=255)                                                                                        aaTCGAGTATGTTGCGAATGTGGATGGCAGGTCAGGGAACCATTTAAATCTCGGACCAAATGAATATCAAAGCCAAGACCGTTTCATCGCATAAAGGTAATATTAAACGTAAGATCAAAACGCATAATAAACAGGTTATc                                >  2:517255/1‑140 (MQ=255)                                                                                             aGTATGTTGCGAATGTGGATGGCAGGTCAGGGAACCATTTAAATCTCTGACCAAATGAATATCAAAGCCAAGACCGTTTCATCGCATAAAGGTAATATTAAACGTAAGATCAAAACGCATAATAAACAGGTTATCTACCa                           <  1:517255/140‑1 (MQ=255)                                                                                                     gCGAATGTGGATGGCTGGTCAGCGATCCATTTAAATCTCTGACCAAATGAATATCAAAGCCAAGACCGTTTCATCGCATAAAGGTAATATTAAACGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCGTcc                   <  1:298890/140‑1 (MQ=255)                                                                                                     gCGAATGTGGATGGCAGGTCAGGGAACCATTTAAATCTCTGACCAAATGAATATCAAAGCCAAGACCGTTTCATCGCATAAAGGTAATATTAAACGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCGTcc                   <  1:558109/140‑1 (MQ=255)                                                                                                      cGAATGTGGATGGCAGGTCAGGGAACCATTTAAATCTCTGACCAAATGAATATCAAAGCCAAGACCGTTTCATCGCATAAAGGTAATATTACACGTAAGACCAAAACGGATAATAAAAAGGTGATCTACCATGTCGTCCg                  >  2:298890/1‑140 (MQ=255)                                                                                                      cGAATGTGGATGGCAGGTCAGGGAACCATTTAAATCTCTGACCAAATGAATATCAAAGCCAAGACCGTTTCATCGCATAAAGGTAATATTAAACGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCGTcc                   <  1:771372/139‑1 (MQ=255)                                                                                                      cGAATGTGGATGGCAGGTCAGGGAACCATTTAAATCTCTGACCAAATGAATATCAAAGCCAAGACCGTTTCATCGCATAAAGGTAATATTAAACGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCGTcc                   >  2:771372/1‑139 (MQ=255)                                                                                                       gAATGTGGATGGCAGGTCAGGGAACCATTTAAATCTCTGACCAAATGAATATCAAAGCCAAGACCGTTTCATCGCATAAAGGTAATATTAAACTTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCGTCCGa                 <  1:137789/140‑1 (MQ=255)                                                                                                       gAATGTGGATGGCAGGTCAGGGAACCATTTAAATCTCTGACCAAATGAATATCAAAGCCAAGACCGTTTCATCGCATAAAGGTAATATTAAACGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCGTCCGa                 <  2:60589/140‑1 (MQ=255)                                                                                                             ggATGGCAGGTCAGGGAACCATTTAAATCTCTGACCAAATGAATATCAAAGCCAAGACCGTTTCATCGCATAAAGGTAATATTAAACGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCGTCCGACTGACg           >  2:772734/1‑140 (MQ=255)                                                                                                                   cAGATCAGGGAACCATTTAAATCTCGGACCAAATGAATATCAAAGCCAAGACCGTTTCATCGCATAAAGGTAATATTAAACGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCGTCCGACTGACGGATAAt     <  2:22607/140‑1 (MQ=255)                                                                                                                     ggTCAGGGAACCATTTAAATCTCTGACCAAATGAATATCAAAGCCAAGACCGTTTCATCGCATAAAGGTAATATTAAACGTAAGATCAAAACTCATAATAAACAGGTTATCTACCATGTCGTCCGACTGACGGATAAtgt   <  2:554590/140‑1 (MQ=255)                                                                                                                      gTCAGGGTACCATTTAAATCTCTGACCAAATGAATATCAAAGCCAAGACCGTTTCATCGCATAAAGGTAATATTAAACGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCGTCCGACTGACGGATAAt     <  1:16016/137‑1 (MQ=255)                                                                                                                      gTCAGGGAACCATTTAAATCTCTGACCAAATGAATATCAAAGCCAAGACCGTTTCATCGCATAAAGGTAATATTAAACGTAAGATCAAAACTCATAATAAACAGCTTAGCTACCATGGCGTCCGACTGACGGATAAtgtg  >  2:247251/1‑140 (MQ=255)                                                                                                                      gTCAGGGAACCATTTAAATCTCTGACCAAATGAATATCAAAGCCAAGACCGTTTCATCGCATAAAGGTAATATTAAACGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCGTCCGACTGACGGATAAtgtg  >  2:779289/1‑140 (MQ=255)                                                                                                                      gTCAGGGAACCATTTAAATCTCTGACCAAATGAATATCAAAGCCAAGACCGTTTCATCGCATAAAGGTAATATTAAACGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCGTCCGACTGACGGATAAt     >  2:16016/1‑137 (MQ=255)                                                                                                                         aGGGAACCATTTAAATCTCTGACCAAATGAATATCAAAGCCAAGACCGTTTCATCGCATAAAGGTAATATTAAACGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCGTCCGa                 <  2:606142/122‑1 (MQ=255)                                                                                                                         aGGGAACCATTTAAATCTCTGACCAAATGAATATCAAAGCCAAGACCGTTTCATCGCATAAAGGTAATATTAAACGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCGTCCGa                 >  1:606142/1‑122 (MQ=255)                                                                                                                                cATTTAAATCTCTGACCAAATGAATATCAAAGCCAAGACCGTTTCATCGCATAAAGGTAATATTAAACGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCGTCCGACTGACGGATa       >  1:54761/1‑125 (MQ=255)                                                                                                                                cATTTAAATCTCTGACCAAATGAATATCAAAGCCAAGACCGTTTCATCGCATAAAGGTAATATTAAACGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCGTCCGACTGACGGATa       <  2:54761/125‑1 (MQ=255)                                                                                                                                    |                                                                                                                              TCGACGATATCCTTGGCGATATTCTGAAAAAAGAGACAACGATAACCTCGTTTTTAAATATGCCGACGTTATCATTGAGCCGAACCGAATCGAGTATGTTGCGAATGTGGATGGCAGGTCAGGGAACCATTCAAATCTCTGACCAAATGAATATCAAAGCCAAGACCGTTTCATCGCATAAAGGTAATATTAAACGTAAGATCAAAACGCATAATAAACAGGTTATCTACCATGTCGTCCGACTGACGGATAATGTG  >  NC_000913/2024305‑2024561

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑