BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,096,351	G→A	G170S (GGT→AGT)	hisF →	imidazole glycerol phosphate synthase subunit HisF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,096,351	0	G	A	100.0%	121.3 / NA	36	G170S (GGT→AGT)	hisF	imidazole glycerol phosphate synthase subunit HisF
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (23/13); total (23/13)

TGGTCGGTATTGATACCTGGTACGACGCCGAAACCGGTAAATATCATGTGAATCAATATACCGGCGATGAAAGCCGCACCCGCGTCACTCAATGGGAAACGCTCGACTGGGTACAGGAAGTGCAAAAACGCGGTGCCGGAGAAATCGTCCTCAATATGATGAATCAGGACGGCGTGCGTAACGGTTACGACCTCGAACAACTGAAAAAAGTGCGTGAAGTTTGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAA  >  NC_000913/2096220‑2096485

tgGTCGGTATTGATACCTGGTACGACGCCGAAACCGGTAAATATCATGTGAATCAATATACCGGCGATGAAAGCCGCACCCGCGTCACTCAATGGGAAACGCTCGACTGGGTACAGGAAGTGCAAAAACGCAGTGCCGga                                                                                                                                >  1:654274/1‑140 (MQ=255)

   tCGGTATTGATACCTGGTACGACGCCGAAACCGGTAAATATCATGTGAATCAATATACCGGCGATGAAAGCCGCACCCGCGTCACTCAATGGGAAACGCTCGACTGGGTACAGGAAGTGCAAAAACGCAGTGCCGGAGaa                                                                                                                             >  1:531416/1‑140 (MQ=255)

    cGGTATTGATACCTGGTACGACGCCGAAACCGGTAAATATCATGTGAATCAATATACCGGCGATGAAAGCCGCACCCGCGTCACTCAATGGGAAACGCTCGACTGGGTACAGGAAGTGCAAAAACGCAGTGCCGGAGaaa                                                                                                                            <  2:654274/140‑1 (MQ=255)

     ggTATTGATACCTGGTACGACGCCGAAACCGGTAAATATCATGTGAATCAATATACCGGCGATGAAAGCCGCACCCGCGTCACTCAATGGGAAACGCTCGCCTGGGTAGAGGAAGTGAAAAAACGCAGTGCCGGAGAAAt                                                                                                                           >  2:277608/1‑140 (MQ=255)

     ggTATTGATACCTGGTACGACGCCGAAACCGGTAAATATCATGTGAATCAATATACCGGCGATGAAAGCCGCACCCGCGTCACTCAATGGGAAACGCTCGACAGGGTACAGGAACTGGAAAAACGCAGTGCCGGAGAAAt                                                                                                                           >  1:231484/1‑140 (MQ=255)

                   gTACGACGCCGAAACCGGTAAACATTATGTGAATCAAGATACCGGCGATGAAAGCCGCACCCGCGTCACTCAATGGGAAACGCTCGACTGGGTACAGGAAGTGCAAAAACGCAGTGCCGGAGAAATCGTCCTCAATatga                                                                                                             <  1:277608/140‑1 (MQ=255)

                    tACGACGCCGAAACCGGTAAATATCATGTGAATCAATATACCGGCGATGAAAGCCGCACCCGCGTCACTCAATGGGAAACGCTCGACTGGGTACAGGAAGTGCAAAAACGCAGTGCCGGAGAAATCGTCCTCAATatgat                                                                                                            >  2:692214/1‑140 (MQ=255)

                                                   aTCAATATACCGGCGATGAAAGCCGCACCCGCGTCACTCAATGGGAAACGCTCGACTGGGTACAGGAAGTGCAAAAACGCAGTGCCGGAGATATCGTCCTCAATATGATGAATCAGGACGGCGTGCGTAACGGTTACGAc                                                                             >  2:127580/1‑140 (MQ=255)

                                                         ataCCGGCGATGAAAGCCGCACCCGCGTCACTCAATGGGAAACGCTCGACTGGGTACAGGAAGTGCAAAAACGCAGTGCCGGAGAAATCGTCCTCAATATGATGAATCAGGACGGCGTGCGTAACGGTTACGACCTCGaa                                                                       >  2:4384/1‑140 (MQ=255)

                                                         ataCCGGCGATGAAAGCCGCACCCGCGTCACACAACGGGTAACGCTCGTCTGGGTACAGGAAGTGCAAAAACGCAGTGCCGGAGAAATCGTCCTCAATATGATGAATCAGGACGGCGTGCGTAACGGTTACGACCTCGaa                                                                       <  2:231484/140‑1 (MQ=255)

                                                          taCCGGCGATGAAAGCCGCACCCGCGTCACTCAATGGGAAACGCTCGACTGGGTACAGGAAGTGCAAAAACGCAGTGCCGGAGAAATCGTCCTCAATATGATGAATCAGGACGGCGTGCGTAACGGTTACGACCTCGaac                                                                      >  2:622774/1‑140 (MQ=255)

                                                             cGGCGATGAAAGCCGCACCCGCGTCACTCAATGGGAAACGCTCGACTGGGTACAGGAAGTGCAAAAACGCAGTGCCGGAGAAATCGTCCTCAATATGATGAATCAGGACGGCGTGCGTAACGGTTACGACCTCg                                                                         <  1:195474/134‑1 (MQ=255)

                                                             cGGCGATGAAAGCCGCACCCGCGTCACTCAATGGGAAACGCTCGACTGGGTACAGGAAGTGCAAAAACGCAGTGCCGGAGAAATCGTCCTCAATATGATGAATCAGGACGGCGTGCGTAACGGTTACGACCTCg                                                                         >  2:195474/1‑134 (MQ=255)

                                                             cGGCGATGAAAGCCGCACCCGCGTCACTCAATGGGAAACGCTCGACTGGGTACAGGAAGTGCAAAAACGCAGTGCCGGAGAAATCGTCCTCAATATGATGAAGCAGGACGGCGTGCGGAACGGTAACGACCTCGaacaac                                                                   >  1:268600/1‑140 (MQ=255)

                                                              ggCGATGAAAGCCGCACCCGCGTCACTCAATGGGAAACGCTCGACTGGGTACAGGAAGTGCAAAAACGCAGTGCCGGAGAAATCGTCCTCAATATGATGAATCAGGACGGCGTGCGTAACGGTTACGACCTCGAACAACt                                                                  >  1:489334/1‑140 (MQ=255)

                                                               gCGATGAAAGCCGCACCCGCGTCACTCAATGGGAAACGCTCGACTGGGTACAGGAAGTGCAAAAACGCAGTGCCGGAGAAATCGTCCTCAATATGATGAATCAGGACGGCGTGCGTAACGGTTACGACCTCGAACAACTg                                                                 >  2:600284/1‑140 (MQ=255)

                                                               gCGATGAAAGCCGCACCCGCGTCACTCAATGGGAAACGCTCGACTGGGTACAGGAAGTGCAAAAACGCAGTGCCGGAGAAATCGTCCTCAATATGATGAATCAGGACGGCGTGCGTAACGGTTACGACCTCGAACAACTg                                                                 >  2:787376/1‑140 (MQ=255)

                                                                cGATGAAAGCCGCACCCGCGTCACTCAATGGGAAACGCTCGACTGGGTACAGGAAGTGCAAAAACGCAGTGCCGGAGAAATCGTCCTCAATATGATGAATCAGGACGGCGTGCGTAACGGTTACGACCTCGAACAACTGa                                                                >  2:823277/1‑140 (MQ=255)

                                                                    gAAAGCCGCACCCGCGTCACTCAATGGGAAACGCTCGACTGGGTACAGGAAGTGCAAAAACGCAGTGCCGGAGAAATCGTCCTCAATATGATGAATCAGGACGGCGTGCGTAACGGTTACGACCTCGAACAACTGaaaaa                                                            >  2:774070/1‑140 (MQ=255)

                                                                     aaaGCCGCACCCGCGTCACTCAATGGGAAACGCTCGACTGGGTACAGGAAGTGCAAAAACGCAGTGCCGGAGAAATCGTCCTCAATATGATGAATCAGGACGGCGTGCGTAACGGTTACGACCTCGAACAACTGaaaaaa                                                           >  1:786938/1‑140 (MQ=255)

                                                                           gCACCCGCGTCACTCAATGGGACACGCTCGACTGGATACTGGAAGTGCCAAAACGCAGTGCCGGAGAAATCGTCCTCAATATGATGAATCAGGACGGCGTGCGTAACGGTTACGACCTCGAACAACTGAAAAAAGTGCGt                                                     <  2:268600/140‑1 (MQ=255)

                                                                             aCCCGCGTCACTCAATGGGAAACGCTCGACTGGGTACAGGAAGTGCAAAAACGCAGTGCCGGAGAAATCGTCCTCAATATGATGAATCAGGACGGCGTGCGTAACGGTTACGACCTCGAACAACTGAAAATAGTGCGTGa                                                   >  2:631617/1‑140 (MQ=255)

                                                                                    tCACTCAATGGGAAACGCTCGACTGGGTACAGGAAGTGCAAAAACGCAGTGCCGGAGAAATCGTCCTCAATATGATGAATCAGGACGGCGTGCGTAACGGTTACGACCTCGAACAACTGAAAAAAGTGCGTGAAGTTTGc                                            <  1:823277/140‑1 (MQ=255)

                                                                                     cACTCAATGGGAAACGCTCGACTGGGTACAGGAAGTGCAAAAACGCAGTGCCGGAGAAATCGTCCTCAATATGATGAATCAGGACGGCGTGCGTAACGGTTACGACCTCGAACAACTGAAAAAAGTGCGTGAAGTTTGcc                                           <  1:622774/140‑1 (MQ=255)

                                                                                         cAATGGGAAACGCTCGACTGGGTACAGGAAGTGCAAAAACGCAGTGCCGGAGAAATCGTCCTCAATATGATGAATCAGGACGGCGTGCGTAACGGTTACGACCTCGAACAACTGAAAAAAGTGCGTGAAGTTTGCCACGt                                       >  2:5604/1‑140 (MQ=255)

                                                                                                  aCGCTCGACTGGGTACAGGAAGTGCAAAAACGCAGTGCCGGAGAAATCGTCCTCAATATGATGAATCAGGACGGCGTGCGTAACGGTTACGACCTCGAACAACTGAAAAAAGTGCGTGAAGTTTGc                                            >  2:132131/1‑126 (MQ=255)

                                                                                                  aCGCTCGACTGGGTACAGGAAGTGCAAAAACGCAGTGCCGGAGAAATCGTCCTCAATATGATGAATCAGGACGGCGTGCGTAACGGTTACGACCTCGAACAACTGAAAAAAGTGCGTGAAGTTTGc                                            <  1:132131/126‑1 (MQ=255)

                                                                                                      tCGACTGGGTACAGGAAGTGCAAAAACGCAGTGCCGGAGAAATCGTCCTCAATATGATGAATCAGGACGGCGTGCGTAACGGTTACGACCTCGAACAACTGAAAAAAGTGCGTGTAGATTGCCAAGTCCCGCTGATTGcc                          >  1:304654/1‑140 (MQ=255)

                                                                                                                    gAAGTGCAAAAACGCAGTGCCGGAGATATCGTCCTCAATATGATGAATCAGGACGGCGTGCGTAACGGTTACGACCTCGAACAACTGAAAAAAGTGCGTGAAGTTTGCCACGTCCCGCTGATTGCCTCCGGTGGCGCggg            <  1:127580/140‑1 (MQ=255)

                                                                                                                    gAAGTGCAAAAACGCAGTGCCGGAGAAATCGTCCTCAATATGATGAATCAGGACGGCGTGCGTAACGGTTACGACCTCGAACAACTGAAAAAAGTGCGTGAAGTTTGCCACGTCCCGCTGATTGCCTCCGGTGGCGCggg            <  2:786938/140‑1 (MQ=255)

                                                                                                                      aGTGCAAAAACGCAGTGCCGGAGAAATCGTCCTCAATATGATGAATCAGGACGGCGTGCGTAACGGTTACGACCTCGAACAACTGAAAAAAGTGCGTGAAGTTTGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCa          <  1:692214/140‑1 (MQ=255)

                                                                                                                      aGTGCAAAAACGCAGTGCCGGAGAAATCGTCCTCAATATGATGAATCAGGACGGCGTGCGTAACGGTTACGACCTCGAACAACTGAAAAAAGTGCGTGAAGTTTGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCa          <  1:774070/140‑1 (MQ=255)

                                                                                                                       gTGCAAAAACGCAGTGCCGGAGAAATCGTCCTCAATATGATGAATCAGGACGGCGTGCGTAACGGTTACGACCTCGAACAACTGAAAAAAGTGCGTGAAGTTTGCCACGTCCCGATGCTTGCCTACGGTGGCGCGGGCAc         >  1:371079/1‑140 (MQ=255)

                                                                                                                         gCAAAAACGCAGTGCCGGAGAAATCGTCCTCAATATGATGAATCAGGACGGCGTGCGTAACGGTTACGACCTCGAACAACTGAAAAAAGTGCGTGAAGTTTGCCATGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCa       >  2:5009/1‑140 (MQ=255)

                                                                                                                            aaaaCGCAGTGCCGGAGAAATCGTCCTCAATATGATGAATCAGGACGGCGTGCGTAACGGTTACGACCTCGAACAACTGAAAAAAGTGCGTGAAGTTTGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATgg    <  1:4384/140‑1 (MQ=255)

                                                                                                                              aaCGCAGTGCCGGAGAAATCGTCCTCAATATGATGAATCAGGACGGCGTGCGTAACGGTTACGACCTCGAACAACTGAAAAAAGTGCGTGAAGTTTGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGaa  >  1:433615/1‑140 (MQ=255)

TGGTCGGTATTGATACCTGGTACGACGCCGAAACCGGTAAATATCATGTGAATCAATATACCGGCGATGAAAGCCGCACCCGCGTCACTCAATGGGAAACGCTCGACTGGGTACAGGAAGTGCAAAAACGCGGTGCCGGAGAAATCGTCCTCAATATGATGAATCAGGACGGCGTGCGTAACGGTTACGACCTCGAACAACTGAAAAAAGTGCGTGAAGTTTGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGGAA  >  NC_000913/2096220‑2096485

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`