Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,124,085	T→C	T146A (ACC→GCC)	cpsB ←	mannose‑1‑phosphate guanylyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,124,085	0	T	C	100.0%	166.4 / NA	50	T146A (ACC→GCC)	cpsB	mannose‑1‑phosphate guanylyltransferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (24/26);  total (24/26)

CTGAGCGGTTTCCAGATTCGGTTTTTCGACAAACTGCGCCACTTCAAAGGCCACCATATCCTGCTCACCCGCAGACACTTCACCGCGACGAATATAGCCATAACCGGTTTCTGGTAGATCCGGCACAATGCCGAAGGTCACCAGCTTGCCCGCTTCGGCATATGGCATGGCATTACGCACGGCGGCACGGAACGCGTCTTCATCGGCAATCACATGATCCGCCGCCAATACCAGCATTAACGGGTCGCTCTCCGGGCTATGACGTTTT  >  NC_000913/2123948‑2124215                                                                                                                                          |                                                                                                                                   cTGAGCGGTTTCCAGATTCGGTTTTTCGACAAACTGCGCCACTTCAAAGGCCACCATATCCTGCTCACCCGCAGACACTTCACCGCGACGAATATAGCCATAACCGGTTTCTGGTAGATCCGGCACAATGCCGAAGGCCa                                                                                                                                  >  1:42174/1‑140 (MQ=255)         tttCCAGATTCGGTTTTTCGACAAACTGCGCCACTTCAAAGGCCACCATATCCTGCTCACCCGCAGACACTTCACCGCGACGAATATAGCCATAACCGGTTTGTGGTAGATCCGACACAATGCCGAAGGCCACCAGCTTg                                                                                                                          >  1:234552/1‑140 (MQ=255)         tttCCAGATTCGGTTTTTCGACAAACTGCGCCACTTCAAAGGCCACCATATCCTGCTCACCCGCAGACACTTCACCGCGACGAATATAGCCATAACCGGTTTGTGGTAGAGCCGACAGAATGCCGAAGGCCACCAGCTTg                                                                                                                          >  1:237082/1‑140 (MQ=255)               gATTCGGTTTTTCGACAAACTGCGCCACTTCAAAGGCCACCATATCCTGCTCACCCGCAGACACTTCACCGCGACGAATATAGCCATAACCGGTTTCTGGTAGATCCGGCACAATGCCGAAGGCCGCCAGCTTGCCCGCt                                                                                                                    <  1:114488/140‑1 (MQ=255)               gATTCGGTTTTTCGACAAACTGCGCCACTTCAAAGGCCACCATATCCTGCTCACCCGCAGACACTTCACCGCGACGAATATAGCCATAACCGGTTTCTGGTAGATCCGGCACAATGCCGAAGGCCACCAGCTTGCCCGCt                                                                                                                    <  1:516944/140‑1 (MQ=255)                aTTCGGTTTTTCGACAAACTGCGCCACTTCAAAGGCCACCATATCCTGCTCACCCGCAGACACTTCACCGCGACGAATATAGCCATAACCGGTTTCTGGTAGATCCGGCACAATGCCGAAGGCCACCAGCTTGCCCGCtt                                                                                                                   <  2:459715/140‑1 (MQ=255)                aTTCGGTTTTTCGACAAACTGCGCCACTTCAAAGGCCACCATATCCTGCTCACCCGCAGACACTTCACCGCGACGAATATAGCCATAACCGGTTTCTGGTAGATCCGGCACAATGCCGAAGGCCACCAGCTTGCCCGCtt                                                                                                                   <  1:491635/140‑1 (MQ=255)                   cGGTTTTTCGACAAACTGCGCCACTTCAAAGGCCACCATATCCTGCTCACCCGCAGACACTTCACCGCGACGAATATAGCCATAACCGGTTTCTGGTAGATCCGGCACAATGCCGAAGGCCACCAGCTTGCCCGCTTCgg                                                                                                                <  2:661744/140‑1 (MQ=255)                       ttttCGACAAACTGCTCCACTTCAACGGCTACCATATACTGCTCACCCGCAGACACTTCACCGCGACGAATATAGCCATAACCGGTTTCTGGTAGATCCGGCACAATGCCGAAGGCCACCAGCTTGCCCGCTTCGGCata                                                                                                            <  1:236144/140‑1 (MQ=255)                       ttttCGACAAACTGCGCTCCTTCAAAGGCCACCATTTCCCGCTCACCCCCAGACCCTTCACCGCGACGAATATAGCCATAACCGGTTTCTGGTAGATCCGGCACAATGCCGAAGGCCACCAGCTTGCCCGCTTCGGCata                                                                                                            <  2:237082/140‑1 (MQ=255)                       ttttCGACAAACTGCGCCACTTCAAAGGCCACCATATCCTGCTCACCCGCAGACACTTCACCGCGACGAATATAGCCATAACCGGTTTCTGGTAGATCCGGCACAATGCCGAAGGCCACCAGCTTGCCCGCTTCGGCata                                                                                                            <  1:728703/140‑1 (MQ=255)                       ttttCGACAAACTGCGCCACTTCAAAGGCCACCATATCCTGCTCACCCGCAGACACTTCACCGCGACGAATATAGCCATAACCGGTTTCTGGTAGATCCGGCACAATGCCGAAGGCCACCAGCTTGCCCGCTTCGGCata                                                                                                            <  2:685201/140‑1 (MQ=255)                       ttttCGACAAACTGCGCCACTTCAAAGGCCACCATATCCTGCTCACCCGCAGACACTTCACCGCGACGAATATAGCCATAACCGGTTTCTGGTAGATCCGGCACAATGCCGAAGGCCACCAGCTTGCCCGCTTCGGCata                                                                                                            >  1:685201/1‑140 (MQ=255)                            gACAAACTGCGCCACTTCAAAGGCCACCATATCCTGCTCACCCGCAGACACTTCACCGCGACGAATATAGCCATAACCGGTTTCTGGTAGATCCGGCACAATGCCGAAGGCCACCAGCTTGCCCGCTTCGGCATatggca                                                                                                       <  1:628951/140‑1 (MQ=255)                              cAAACTGCGCCACTTCAAAGGCCACCATATCCTGCTCACCCGCAGACACTTCACCGCGACGAATATAGCCATAACCGGTTTCTGGTAGATCCGGCACAATGCCGAAGGCCACCAGCTTGCCCGCTTCGGCATatggcatg                                                                                                     <  1:699386/140‑1 (MQ=255)                                           ttCAAAGGCCACCATATCCTGCTCACCCGCAGACACTTCACCGCGACGAATATAGCCATAACCGGTTTCTGGTAGATCCGGCACAATGCCGAAGGCCACCAGCTTGCCCGCTTCGGCATATGGCATGGCATTACGCAcgg                                                                                        >  2:524982/1‑140 (MQ=255)                                           ttCAAAGGCCACCATATCCTGCTCACCCGCACACACTTCGCCGCGACGTATATAGCCATAACCGGTTTCTGGTAGATCCGGCACAATGCCGAAGGCCACCAGCTTGCCCGCTTCGGCATATGGCATGGCATTACGCAcgg                                                                                        <  2:234552/140‑1 (MQ=255)                                                   ccaccaTATCCTGCTCACCCGCAGACACTTCACCGCGACGAATATAGCCATAACCGGTTTCTGGTAGATCCGGCACAATGCCGAAGGCCACCAGCTTGCCCGCTTCGGCATATGGCATGGCATTACGCACGGCGGCACgg                                                                                <  2:116767/140‑1 (MQ=255)                                                     accaTATCCTGCTCACCCGCAGACACTTCACCGCGACGAATATAGCCATAACCGGTTTCTGGTAGATCCGGCACAATGCCGAAGGCCACCAGCTTGCCCGCTACGGCATAAGGCCTGGCATTACTCACGGCGGCACGGaa                                                                              >  1:309408/1‑140 (MQ=255)                                                                cTCACCCGCAGACACTTCACCGCGACGAATATAGCCATAACCGGTTTCTGGTAGATCCGGCACAATGCCGAAGGCCACCAGCTTGCCCGCTTCGGCATATGGGATGGCATGACGGACTGCGGCAGGGAACGCGTCTTCAt                                                                   >  1:232647/1‑140 (MQ=255)                                                                cTCACCCGCAGACACTTCACCGCGACGAATATAGCCATAACCGGTTTCTGGTAGATCCGGCACAATGCCGAAGGCCACCAGCTTGCCCGCTTCGGCATATGGCATGGCATTACGCACGGCGGCACGGAACGCGTCTTCAt                                                                   >  1:859357/1‑140 (MQ=255)                                                                 tCACCCGCAGACACTTCACCGCGACGAATATAGCCATAACCGGTTTCTGGTAGATCCGGCACAATGCCGAAGGCCACCAGCTTGCCCGCTTCGGCATATGTCATTGGACTACGCACGGCGGCCCGGAACGCGTCTTCATc                                                                  >  2:381181/1‑140 (MQ=255)                                                                 tCACCCGCAGACACTTCACCGCGACGAATATAGCCATAACCGGTTTCTGGTAGATCCGGCACAATGCCGAAGGCCACCAGCTTGCCCGCTTCGGCATATGGCATGGCATTACGCACGGCGGCACGGAACGCGTCTTCATc                                                                  >  2:3296/1‑140 (MQ=255)                                                                  cACCCGCAGACACTTCACCGCGACGAATATAGCCATAACCGGTTTCTGGTAGATCCGGCACAATGCCGAAGGCCACCAGCTTGCCCGCTTCGGCATATGGCATGGCATTACGCACGGCGGCACGGAACGCGTCTTCATCg                                                                 <  2:42174/140‑1 (MQ=255)                                                                   ccccGCAGACACTTCACCGCGATGAATATAGCCATTACCCGTTTCTGGCAGATCCGGCACAATGCCGAAGGCCACCAGCTTGCCCGCTTCGGCATATGGCATGGCATTACGCACGGCGGCACGGAACGCGTCTTCATCgg                                                                <  2:232647/139‑1 (MQ=255)                                                                     ccGCAGACACTTCACCGCGACGAATATAGCCATAACCGGTTTCTGGTAGATCCGGCACAATGCCGAAGGCCACCAGCTTGCCCGCTTCGGCATATGGCATGGCATTACGCGCGGGGGCACGGAACGCGTCTTCATCGGCa                                                              >  1:296666/1‑140 (MQ=255)                                                                     ccGCAGACACTTCACCGCGACGAATATAGCCATAACCGGTTTCTGGTAGATCCGGCACAATGCCGAAGGCCACCAGCTTGCCCGCTTCGGCATATGGCATGGCATTACGCACGGCGGCACGGAACGCGTCTTCATCGGCa                                                              >  2:92936/1‑140 (MQ=255)                                                                     ccGCAGACACTTCACCGCGACGAATATAGCCATAACCGGTTTCTGGTAGATCCGGCACAATGCCGAAGGCCACCAGCTTGCCCGCTTCGGCATATGGCATGGCATTACGCACGGCGGCACGGAACGCGTCTTCATCGGCa                                                              >  2:165210/1‑140 (MQ=255)                                                                       gCAGACACTTCACCGCGACGAATATAGCCATAACCGGTTTCTGGTAGATCCGGCACAATGCCGAAGGCCACCAGCTTGCCCGCTTCGGCATATGGCATGGCATTACGCACGGCGGCACGGAACGCGTCTTCATCGGCAAt                                                            >  2:36889/1‑140 (MQ=255)                                                                          gACACTTCACCGCGACGAATATAGCCATAACCGGTTTCTGGTAGATCCGGCACAATGCCGAAGGCCACCAGCTTGCCTGCTTCGGCATATGGCATGGCATTACGCACGGCGGCACGGAACGCGTCTTCATCGGCAATcac                                                         >  2:479713/1‑140 (MQ=255)                                                                               ttCACCGCGACGAATATAGCCATAACCGGTTTCTGGTAGATCCGGCACAATGCCGAAGGCCACCAGCTTGCCCGCTTCGGCATATGGCATGGCATTACGCACGGCGGCACGGAACGCGTCTTCATCg                                                                 <  1:725715/127‑1 (MQ=255)                                                                               ttCACCGCGACGAATATAGCCATAACCGGTTTCTGGTAGATCCGGCACAATGCCGAAGGCCACCAGCTTGCCCGCTTCGGCATATGGCATGGCATTACGCACGGCGGCACGGAACGCGTCTTCATCg                                                                 >  2:725715/1‑127 (MQ=255)                                                                                  cccGCGACGAATATAGCCATAACCGGTTTCTCGTAAATCCGGCACAATACCGAAGGCCACCAGCTTGCCCGCTTCGGCATATGGCATGGCATTACGCACGGCGGCACGGAACGCGTCTTCATCGGCAATCACATGATccg                                                 <  2:296666/139‑1 (MQ=255)                                                                                      cgacgaATATAGCCATAACCGGTTTCTGGTAGATCCGGCACAATGCCGAAGGCCACCAGCTTGCCCGCTTCGGCATATGGCATGGCATTACGCACGGCGGCACGGAACGCGTCTTCATCGGCAATCACATGATCcgccgc                                             >  2:499947/1‑140 (MQ=255)                                                                                         cgaATATAGCCATAACCGGTTTCTGGTAGATCCGGCACAATGCCGAAGGCCACCAGCTTGCCCGCTTCGGCATATGGCATGGCATTACGCAGGGCGGCACAGAATGGGAATTCATCGACAATGTCATGATCCGCCGCCaa                                          >  2:405978/1‑140 (MQ=255)                                                                                         cgaATATAGCCATAACCGGTTTCTGGTAGATCCGGCACAATGCCGAAGGCCACCAGCTTGCCCGCTTCGGCATATGGCATGGCATTACGCACGGCGGCACGGAACGCGTCTTCATCGGCAATCACATGATCCGCCGCCaa                                          >  1:153459/1‑140 (MQ=255)                                                                                            atatAGCCTCATCCGGTTTCTGGTAGATCCGGCACAATGCGGAGGGACACCAGCTTGCCCGCTTCGGCTTATGGCATGGCATTACGCCCGGCGGAACGGAACGCGTCTTCATCGGCAATCACATGATCCGCCGCCAATAc                                       <  2:859357/140‑1 (MQ=255)                                                                                            atatAGCCATAACCGGTTTCTGGTAGATCGGGCACAATGCCGAAGGCCGCCAGCTTGCCCGCTTCGGCATATGGCATGGCATTACGCACGGCGGCACGGAACGCGTCTTCATCGGCAATCACATGATCCGCCGCCAATAc                                       <  1:532390/140‑1 (MQ=255)                                                                                            atatAGCCATAACCGGTTTCTGGTAGATCCGGCACAATGCCGAAGGCCACCAGCTTGCCCGCTTCGGCATATGGCATGGCATTACGCACGGCGGCACGGAACGCGTCTTCATCGGCAATCACATGATCCGCCGCCAATAc                                       <  1:479713/140‑1 (MQ=255)                                                                                            atatAGCCATAACCGGTTTCTGGTAGATCCGGCACAATGCCGAAGGCCACCAGCTTGCCCGCTTCGGCATATGGCATGGCATTACGCACGGCGGCACGGAACGCGTCTTCATCGGCAATCACATGATCCGCCGCCAATAc                                       <  1:92936/140‑1 (MQ=255)                                                                                                     tAACCGGTTTCTGGTAGATCCGGCACAATGCCGAAGGCCACCAGCTTGCCCGCTTCGGCATATGGCATGGCATTACGCACGGCGGCACGGAACGCGTCTTCATCGGCAATCACATGATCCTCCGCCAATACCAg                                    <  2:166114/134‑1 (MQ=255)                                                                                                     tAACCGGTTTCTGGTAGATCCGGCACAATGCCGAAGGCCACCAGCTTGCCCGCTTCGGCATATGGCATGGCATTACGCACGGCGGCACGGAACGCGTCTTCATCGGCAATCACATGATCCTCCGCCAATACCAg                                    >  1:166114/1‑134 (MQ=255)                                                                                                      aaCCGGTTTCTGGTAGATCCGGCACAATGCCGAAGGCCACCAGCTTGCCCGCTTCGGCATATGGCATGGCATTACGCACGGCGGCACGGAAAGCGTCTTCATCGACAAGAACATGATGCGCCCGCAATACCGGCATTAAc                             >  2:420735/1‑140 (MQ=255)                                                                                                          ggTTTCTGGTAGATCCGGCACAATGCCGAAGGCCACCAGCTTGCCCGCTTCGGCATATGGCATGGCATTACGCACGGCGGCACGGAACGCGTCTTCATCGGCAATCACATGATCCGCCGCCAATACCAGCATTAACGGGt                         <  2:209077/140‑1 (MQ=255)                                                                                                           gTTTCTGGTAGATCCGGCACAATGCCGAAGGCCACCAGCTTGCCCGCTTCGGCATATGGCATGGCATTACGCACGGCGGCACGGAACGCGTCTTCATCGGCAATCACATGATCCGCCGCCAATACCAGCATTAACGGGTc                        <  1:590588/140‑1 (MQ=255)                                                                                                            tttCTGGTAGATCCGGCACAATGCCGAAGGCCACCAGCTTGCCCGCTTCGGCATATGGCATGGCATTACGCACGGCGGCACGGAACGCGTCTTCATCGGCAATCAGATGATCCGCCGCCAATACCAGCATTAACGGGTCg                       <  1:165210/140‑1 (MQ=255)                                                                                                                tGGTAGATCCGGCACAATGCCGAAGGCCACCAGCTTGCCCGCTTCGGCATATGGCATGGCATTACGCACGGCGGCACGGAACGCGTCTTCATCGGCAATCACATGATCCGCCGCCAATACCAGCATTAACGGGTCGctct                   <  1:36889/140‑1 (MQ=255)                                                                                                                 ggTAGATCCGGCACAATGCCGAAGGCCACCAGCTTGCCCGCTTCGGCATATGGCATGGCATTACGCACGGCGGCACGGAACGCGTCTTCATCGGCAATCACATGATCCGCCGCCAATACCAGCATTAACGGGTCGCtctc                  <  1:524982/140‑1 (MQ=255)                                                                                                                                 tGCCGAAGGCCACCAGCTTGCCCGCTTCGGCATATGGCATGGCATTACGCACGGCGGCACGGAACGCGTCTTCATCGGCCGTCACATGATCAGCCGCCAATACCAGCATTAACGGGTCGCTCTCCGGGCTATGACGtttt  >  1:11723/1‑140 (MQ=255)                                                                                                                                 tGCCGAAGGCCACCAGCTTGCCCGCTTCGGCATATGGCATGGCATTACGCACGGCGGCACGGAACGCGTCTTCATCGGCAATCACATGATCCGCCGCCAATACCAGCATTAACGGGTCGCTCTCCGGGCTATGACGtttt  >  1:837811/1‑140 (MQ=255)                                                                                                                                 tGCCGAAGGCCACCAGCTTGCCCCCTTCGGCATATGGCATGGCATTACGCACGGCGGCACGGAACGCGTCTTCATCGGCAATCACATGATCCGCCGCCAATACCAGCATTAACGGGTCGCTCTCCGGGCTATGACGtttt  >  1:526560/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                   CTGAGCGGTTTCCAGATTCGGTTTTTCGACAAACTGCGCCACTTCAAAGGCCACCATATCCTGCTCACCCGCAGACACTTCACCGCGACGAATATAGCCATAACCGGTTTCTGGTAGATCCGGCACAATGCCGAAGGTCACCAGCTTGCCCGCTTCGGCATATGGCATGGCATTACGCACGGCGGCACGGAACGCGTCTTCATCGGCAATCACATGATCCGCCGCCAATACCAGCATTAACGGGTCGCTCTCCGGGCTATGACGTTTT  >  NC_000913/2123948‑2124215

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑