Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,127,723 T→C T198A (ACC→GCC)  gmd ← GDP‑mannose 4,6‑dehydratase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,127,7230TC98.0% 169.2 / NA 51T198A (ACC→GCC) gmdGDP‑mannose 4,6‑dehydratase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (23/27);  minor base G (1/0);  total (24/27)
Fisher's exact test for biased strand distribution p-value = 4.71e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CATCATCCACTGCATTTTTACGTAGTCTTTGGCGTGGCCCCAGTCACGCAGGGAATCCATATTGCCGAGGTACAGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGATTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGACCG  >  NC_000913/2127595‑2127842
                                                                                                                                |                                                                                                                       
catcatCCACTGCATTTTTACGTAGTCTTTGGCGTGGCCCCAGTCACGCAGGGAATCCATATTGCCGAGGTACAGGCACGACTCCAGCCCCTGGGCGATGTTAGCGATTGCGCGGGTGATTTTGAGGGCAACGAAGGttt                                                                                                              >  1:317089/1‑140 (MQ=255)
        aCTGCATTTTTACGTTGTCTTTTGCTTGGTCCCAGTCCCGCAGGGAATCCATATTGCCGAGGTACAGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGCAACGAAGGTTTCGCcgcgg                                                                                                      <  1:254521/140‑1 (MQ=255)
          ggCATTTTTACGTAGTCTTTGGCGTGGCCCCAGTCGCGCCGGGAATCCCTATTGCCGAGGTACAGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGCAACGAAGGTTTCGCcgcggcg                                                                                                    <  2:262730/139‑1 (MQ=255)
            gaTTTTTACGTAGTCATTGGCGTGGTCCCAGTCACGCCGGGAATCCATATTGCCGAGGTACAGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGCAACGAAGGTTTCGCcgcggcgcg                                                                                                  <  1:324854/139‑1 (MQ=255)
            cATTTTTACGTAGTCTTTGGCGTGGCCCCAGTCACGCAGGGAATCCATATTGCCGAGGTACAGGCACGACTCCAG‑CCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTTCGGGCATCGAAGGTTTCGCcgcggcgcgg                                                                                                 >  1:373930/1‑140 (MQ=255)
             aTTTTTACGTAGTCTTTGGCGTGGCCCCAGTCACGCAGGGAATCCATATTGCCGAGGTACAGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGCAACGAAGGTTTCGCcgcggcgcgg                                                                                                 <  1:747002/140‑1 (MQ=255)
                   aCGTAGTCTTTGGCGTCGCCCCTGTCACGCAGGCAATCCATATTGCCGAGGTACAGGCACGACTCCAG‑CCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGCAACGAAGGTTTCGCCGCGGCGCGGGGATTCa                                                                                          <  2:373930/140‑1 (MQ=255)
                    cGTAGTCTTTGGCGTGGCCCCAGTCACGCAGGGAATCCATATTGCCGAGGTACAGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGCAACGAAGGTTTCGCCGCGGCGCGGGGATTCa                                                                                          <  1:517040/140‑1 (MQ=255)
                      tAGTCTTTGGCGTGGCCCCAGTCACGCAGGGAATCCATATTGCCGAGGTACAGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGCAACGAAGGTTTCGCCGCGGCGCGGGGATTCATg                                                                                        >  2:508637/1‑140 (MQ=255)
                      tAGTCTTTGGCGTGGCCCCAGTCACGCAGGGAATCCATATTGCCGAGGTACAGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGCAACGAAGGTTGCGCCGCTGCGCGGGGATTCAtc                                                                                        >  1:262730/1‑139 (MQ=255)
                      tAGTCTTTGGCGCCTCCCCAGTCACGCAGGGAATCCATATTGCCCAGGTACAGGCACGACTCCGGCCCCTGCGCGATGTTGGCGATTTCCCTGGTGACTTGGCGACAGGCGCCGGTTCCGCCGCGGCGCgggggcgcatg                                                                                        >  2:372440/1‑133 (MQ=255)
                        gTCTTTGGCGTGGCCCCAGTCACGCAGGGAATCCATATTGCCGAGGTACAGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGAGGGCAACAAAGGTTTCACCGCGGCGCGGGGATTCATggc                                                                                      >  2:324854/1‑139 (MQ=255)
                            ttGGCGTGGCCCCAGTCACGCAGGGAATCCATATTGCCGAGGTACAGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGCAACGAAGGTTTCGCCGCGGCGCGGGGATTCATGGTTGaa                                                                                  <  2:781768/140‑1 (MQ=255)
                            ttGGCGTGGCCCCAGACACGCATGGTATCCATATTGCTGAGGTACAGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGCAACGAAGGTTTCGCCGCGGCGCGGGGATTCATGGTTGaa                                                                                  <  1:251621/140‑1 (MQ=255)
                             tGGCGTGGCCCCAGTCACGCAGGGAATCCATATTGCCGAGGTACAGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGCAACGAAGGTTTCGCTGCGGCGCGGGGATTCATGGTTGAag                                                                                 >  1:396414/1‑140 (MQ=255)
                                cGTGGCCCCAGTCACGCAGGGAATCCATATTGCCGAGGTACAGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGCAACGAAGGTTTCGCCGCGGCGCGGGGATTCATGGTTGAAgaga                                                                              >  2:840675/1‑140 (MQ=255)
                                   ggCCCCAGTCACGCTGGGAATCCATATTGCCGAGGTACAGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGCAACGAAGGTTTCGCCGCGGCGCGGGGATTCATGGTTGAAGAGAAtt                                                                           >  2:35237/1‑140 (MQ=255)
                                   ggCCCCAGTCACGCAGGGAATCCATATTGCCGAGGTACAGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGCAACGAAGGTTTCGCCGCGGCGCGGGGATTCATGGTTGAAGAGAAtt                                                                           >  2:449678/1‑140 (MQ=255)
                                   ggCCCCAGTCACGCAGGGAATCCATATTGCCGAGGTACAGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGCAACGAAGGTTTCGCCGCGGCGCGGGGATTCATGGTTGAAGAGAAtt                                                                           >  1:100616/1‑140 (MQ=255)
                                     ccccAGTCACGCAGGGAATCCATATTGCCGAGGTACAGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGGAACGAAGGGTTCGCCGGGGCGCGGGCATTCATGGTTGAAGAGAATTcc                                                                         >  2:251621/1‑140 (MQ=255)
                                         aGTCACGCAGGGAATACATATTCCCCAGGTACAGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGCAACGAAGGTTTCGCCGCGGCGCGGGGATTCATGGTTGAAGAGAATTCCGTTa                                                                     <  1:339668/140‑1 (MQ=255)
                                                     aaTCCATATTGCCGAGGTACAGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGCAACGAAGGTTTCGCCGCGGCGCGGGGATTCATGGTTGAAGAGAATTCCg                                                                        >  1:686743/1‑125 (MQ=255)
                                                     aaTCCATATTGCCGAGGTACAGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGCAACGAAGGTTTCGCCGCGGCGCGGGGATTCATGGTTGAAGAGAATTCCg                                                                        <  2:686743/125‑1 (MQ=255)
                                                     aaTCCATATTGCCGAGGTACAGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGCAACGAAGGTTTCGCCGCGGCGCGGGGATTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATg                                                         >  1:708608/1‑140 (MQ=255)
                                                     aaTCCATATTGCCGAGGTACAGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGCAACGAAGGTTTCGCCGCGGCGCGGGGATTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATg                                                         >  1:499356/1‑140 (MQ=255)
                                                       tCCATATTGCCGAGGTACAGGCCCGACTCCAGCCCCTTGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGCAACGAAGGTTTCGCCGCGGCGCGGGGATTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGcc                                                       <  1:252253/140‑1 (MQ=255)
                                                       tCCATATTGCCGAGGTACAGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGCAACGAAGGTTTCGCCGCGGCGCGGGGATTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGcc                                                       >  2:861015/1‑140 (MQ=255)
                                                         cATATTGCCGAGGTACAGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGCAACGAAGGTTTCGCCGCGGCGCGGGGATTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGt                                                     <  1:449678/140‑1 (MQ=255)
                                                          atatTGCCGAGGTACAGTCACGTCTCCAGCCACTGTGCGGTGTTGGCGCTTGCGCGGGTGATTTTGCGGGCAACGAAGGTTTCGCCGCGGCGCGGGGATTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTa                                                    <  2:294808/140‑1 (MQ=255)
                                                             ttGCCGAGGTACAGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGCAACGAAGGTTTCGCCGCGGCGCGGGGATTCATGGTTGAAGAGAATTCCGTTACAGGCGTACa                                                           >  1:864311/1‑130 (MQ=255)
                                                             ttGCCGAGGTACAGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGCAACGAAGGTTTCGCCGCGGCGCGGGGATTCATGGTTGAAGAGAATTCCGTTACAGGCGTACa                                                           <  2:864311/130‑1 (MQ=255)
                                                                   aGGTACAGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGCAACGAAGGTTTCGCCGCGGCGCGGGGATTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACg                                           <  1:785643/140‑1 (MQ=255)
                                                                   aGGTACAGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGCAACGAAGGTTTCGCCGCGGCGCGGGGATTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACg                                           <  1:35237/140‑1 (MQ=255)
                                                                     gTACAGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGCAACGAAGGTTTCGCCGCGGCGCGGGGATTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACg                                           >  2:202713/1‑138 (MQ=255)
                                                                     gTACAGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGCAACGAAGGTTTCGCCGCGGCGCGGGGATTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACg                                           <  1:202713/138‑1 (MQ=255)
                                                                             aCGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGCAACGAAGGTTTCGCCGCGGCGCGGGGATTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACg                                 <  1:840675/140‑1 (MQ=255)
                                                                                aCTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGCAACGAAGGTTTCGCCGCGGCGCGGGGATTCATGGTTGAAGAGGATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTg                              >  2:221432/1‑140 (MQ=255)
                                                                                    cAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGCAACGAAGGTTTCGCCGCGGCGCGGGGATTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATcc                          <  1:861015/140‑1 (MQ=255)
                                                                                     aGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGCAACGAAGGTTTCGCCGCGGCGCGGGGATTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGtt                                     <  2:178382/128‑1 (MQ=255)
                                                                                     aGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGCAACGAAGGTTTCGCCGCGGCGCGGGGATTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGtt                                     >  1:178382/1‑128 (MQ=255)
                                                                                     aGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGCAACGAAGGTTTCGCCGCGGCGCGGGGATTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCa                         >  1:208760/1‑140 (MQ=255)
                                                                                      gCCCCTGGGCGATGTTGGCGATAGCGCGGGTGATTATGCGGGCAACGAAGGTTTCGCCGCGGCGCGGGGATTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAg                        <  2:317089/140‑1 (MQ=255)
                                                                                           tGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGCAACGAAGGTTTCGCCGCGGCGCGGGGATTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACg                                 <  2:434818/126‑1 (MQ=255)
                                                                                           tGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGCAACGAAGGTTTCGCCGCGGCGCGGGGATTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACg                                 >  1:434818/1‑126 (MQ=255)
                                                                                            gggCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGCAACGAAGGTTTCGCCGCGGCGCGGGGATTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCTGTAGGATGCACAGTGGTTAACGGTGATCCAGTAGGCg                  >  1:229113/1‑140 (MQ=255)
                                                                                            gggCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGCAACGAAGGTTTCGCCGCGGCGCGGGGATTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAg                        <  1:823491/134‑1 (MQ=255)
                                                                                            gggCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGCAACGAAGGTTTCGCCGCGGCGCGGGGATTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAg                        >  2:823491/1‑134 (MQ=255)
                                                                                             ggCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGCAACGAAGGTTTCGCCGCGGCGCGGGGATTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGt                 <  2:100616/140‑1 (MQ=255)
                                                                                                          aTTGCGCGGGTGATTTTGCGGGCAACGAAGGTTTCGCCGCGGCGCGGGGATTCATGGTTGAAGAGAATTCCGTTCCAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGAc    <  2:499356/140‑1 (MQ=255)
                                                                                                          aTTGCGCGGGTGATTTTGCGGGCAACGAAGGTTTCGCCGCGGCGCGGGGATTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGAc    <  2:429012/140‑1 (MQ=255)
                                                                                                          aTTGCGCGGGTGATTTTGCGGGCAACGAAGGTTTCGCCGCGGCGCGGGGATTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGAc    <  2:708608/140‑1 (MQ=255)
                                                                                                            tGCGCGGGTGATTTTGCGGGCAACGAAGGTTTCGCCTCGGCGCGGGGATTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGACCg  >  1:842343/1‑140 (MQ=255)
                                                                                                            tGCGCGGGTGATTTTGCGGGCAACGAAGGTTTCGCCGCGGCGCGGGGATTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGACCg  >  2:683260/1‑140 (MQ=255)
                                                                                                                                |                                                                                                                       
CATCATCCACTGCATTTTTACGTAGTCTTTGGCGTGGCCCCAGTCACGCAGGGAATCCATATTGCCGAGGTACAGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGATTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGACCG  >  NC_000913/2127595‑2127842

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑