Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	256,049	C→T	P25S (CCT→TCT)	gpt →	xanthine‑guanine phsophoribosyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	256,049	0	C	T	100.0%	175.6 / NA	54	P25S (CCT→TCT)	gpt	xanthine‑guanine phsophoribosyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (24/30);  total (24/30)

TCGCGCAACCTATTTTCCCCTCGAACACTTTTTAAGCCGTAGATAAACAGGCTGGGACACTTCACATGAGCGAAAAATACATCGTCACCTGGGACATGTTGCAGATCCATGCACGTAAACTCGCAAGCCGACTGATGCCTTCTGAACAATGGAAAGGCATTATTGCCGTAAGCCGTGGCGGTCTGGTACCGGGTGCGTTACTGGCGCGTGAACTGGGTATTCGTCATGTCGATACCGTTTGTATTTCCAGCTACGATCACGACAACCAGCG  >  NC_000913/255912‑256182                                                                                                                                          |                                                                                                                                      tcGCGCAACCTATTTTCCCCTCGAACACTTTTTAAGCCGTAGATAAACAGGCTGGGACACTTCACATGAGCGAAAAATACATCGTCACCTGGGACATGTTGCAGATCCATGCACGTAAACTCGCAAGCCGACTGATGTCt                                                                                                                                     >  1:808998/1‑140 (MQ=255)    cgcAACCTATTTTCCCCTCGAACACTTTTTAAGCCGTAGATAAACAGGCTGGGACACTTCACATGAGCGAAAAATACATCGTCACCTGGGACATGTTGCAGATCCATGCACGTAAACTCGCAAGCCGACTGATGtcttct                                                                                                                                  >  2:179954/1‑140 (MQ=255)    cgcAACCTATTTTCCCCTCGAACACTTTTAAAGCCGTAGATAAACAGGCTGGGACACTTCACATGAGCGAAAAATACATCGTCACCTGGGACATGTTGCAGATCCATGCACGTAAACTCGCAAGCCGACTGATGtcttct                                                                                                                                  >  2:197088/1‑140 (MQ=255)                tCCCCTCGAACACTTTTTAAGCCGTAGATAAACAGGCTGGGACACTTCACATGAGCGAAAAATACATCGTCACCTGGGACATGTTGCAGATCCATGCACGTAAACTCGCAAGCCGACTGATGTCTTCTGAACAATGGaaa                                                                                                                      <  2:808998/140‑1 (MQ=255)                   ccTCGAACACTTTTTAAGCCGTAGATAAACAGGCTGGGACACTTCACATGAGCGAAAAATACATCGTCACCTGGGACATGTTGCAGATCCATGCACGTAAACTCGCAAGCCGACTGATGTCTTCTGAACAATGGAAAGGc                                                                                                                   >  2:825326/1‑140 (MQ=255)                      cGAACACTTTTTAAGCCGTAGATAAACAGGCTGGGACACTTCACATGAGCGAAAAATACATCGTCACCTGGGACATGTTGCAGATCCATGCACGTAAACTCGCAAGCCGACTGATGTCTTCTGAACAATGGAAAGGCatt                                                                                                                >  1:721128/1‑140 (MQ=255)                       gatcacTTTTTAAGCCGTAGATAAACAGGCTGGGACACTTCACATGAGCGAAAAATACATCGTCACCTGGGACATGTTGCAGATCCATGCACGTAAACTCGCAAGCCGACTGATGTCTTCTGAACAATGGAAAGGCatta                                                                                                               <  2:530752/137‑1 (MQ=255)                       gacagcTTTTTAAGCCGTAGATCAACAGGATGGGACACTTCACATGAGCGAAAAATACATCGTCACCTGGGACATGTTGCAGATCCATGCACGTAAACTCGCAAGCCGACTGATGTCTTCTGAACAATGGAAAGGCatta                                                                                                               <  1:249280/135‑1 (MQ=255)                       gAACACTTTTTAAGCCGTAGATGAACAGGATGGGACACTTCACATGAGCGAAAAATACATCGTCACCTGGGACATGTTGCAGATCCATGCACGTAAACTCGCAAGCCGACTGATGTCTTCTGAACAATGGAAAGGCatta                                                                                                               <  1:298081/140‑1 (MQ=255)                       gAACACTTTTTAAGCCGTAGATCAACAGGCTGGGACACTTCACATGAGCGAAAAATACATCGTCACCTGGGACATGTTGCAGATCCATGTACGTAAACTCGCAAGCCGACTGATGTCTTCTGAACAATGGAAAGGCatta                                                                                                               <  1:233929/140‑1 (MQ=255)                       gAACACTTTTTAAGCCGTAGATAAACAGGCTGGGACACTTCACATGAGCGAAAAATACATCGTCACCTGGGACATGTTGCAGATCCATGCACGTAAACTCGCAAGCCGACTGATGTCTTCTGAACAATGGAAAGGCatta                                                                                                               <  2:553661/140‑1 (MQ=255)                                    gCCGTAGATAAACAGGCTGGGACACTTCACATGAGCGAAAAATACATCGTCACCTGGGACATGTTGCAGATCCATGCACGTAAACTCGCAAGCCGACTGATGTCTTCTGAACAATGGAAAGGCATTATTGCCGTAAGCCg                                                                                                  <  1:680938/140‑1 (MQ=255)                                       gTAGATAAACAGGCTGGGACACTTCACATGAGCGAAAAATACATCGTCACCTGGGACATGTTGCAGATCCATGCACGTAAACTCGCAAGCCGACTGATGTTTTCAGAAGAATGGAAAAGCATTATTGCCGTAAGcctgtc                                                                                               >  2:249280/1‑136 (MQ=255)                                       gTAGATAAACAGGCTGGGACACTTCACATGAGCGAAAAATACATCGTCACCTGGGACATGTTGCAGATCCATGCACGTAAACTCGCAAGCCGACTGATGTCTTCTGAACAATGGAAAGGCATTATTGCCGTAAGCCGTgg                                                                                               <  2:721128/140‑1 (MQ=255)                                       gTAGATAAACAGGCTGGGACACTTCACATGAGCGAAAAATACATCGTCACCTGGGACATGTTGCAGATCCATGCACGTAAACTCGCAAGCCGACTGATGTCTTCTGAACAATGGAAAGGCATTATTGCCGTAAGCCGTgg                                                                                               <  1:862266/140‑1 (MQ=255)                                       gTAGATAAACAGGCTGGGACACTTCACATGAGCGAAAAATACATCGTCACCTGGGACATGTTGCAGATCCATGCACGTAAACTCGCAAGCCGACTGATGTCTTCTGAACAATGGAAAGGCATTATTGCCGTAAGCCGTgg                                                                                               <  2:537958/140‑1 (MQ=255)                                       gTAGATAAACAGGCTGGGACACTTCACATGAGCGAAAAATACATCGTCACCTGGGACATGTTGCAGATCCATGCACGTAAACTCGCAAGCCGACTGATGTCTTCTGAACAATGGAAAGGCATTATTGCCGTAAGCCGTgg                                                                                               >  1:477772/1‑140 (MQ=255)                                                   gCTGGGACACTTCACATGAGCGAAAAATACATCGTCACCTGGGACATGTTGCAGATCCATGCACGTAAACTCGCAAGCCGACTGATGTCTTCTGAACAATGGAAAGGCATTATTGCCGTAAGCCGTGGCGGTc                                                                                          >  2:527604/1‑133 (MQ=255)                                                   gCTGGGACACTTCACATGAGCGAAAAATACATCGTCACCTGGGACATGTTGCAGATCCATGCACGTAAACTCGCAAGCCGACTGATGTCTTCTGAACAATGGAAAGGCATTATTGCCGTAAGCCGTGGCGGTc                                                                                          <  1:527604/133‑1 (MQ=255)                                                     tGGGACACTTCACATGAGCGAAAAATACATCGTCACCTGGGACATGTTGCAGATCCATGCACGTAAACTCGCAAGCCGACTGATGTCTTCTGAACAATGGAAAGGCATTATTGCCGTAAGCCGTGGCGGTCTGGTACCgg                                                                                 <  1:825326/140‑1 (MQ=255)                                                       ggACACTTCACATGAGCGAAAAATACATCGTCACCTGGGACATGTTGCAGATCCATGCACGTAAACTCGCAAGCCGACTGATGTCTTCTGAACAATGGAAAGGCATTATTGCCGTAAGCCGTGGCGGTCTGGTACCgggg                                                                               >  1:606205/1‑139 (MQ=255)                                                             ttCACATGAGCGAACAATACCTCGTCACCTGGGACATGTTGCAGATCCATGCACGTAAACTCGCAAGCCGACTGATGTCTTCTGAACAATGGAAAGGCATTATTGCCGTACGCCGTGGCGGTTTGGTACAGTGTGCCTTa                                                                         >  2:713161/1‑140 (MQ=255)                                                             ttCACATGAGCGAAAAATACATCGTCACCTGGGACATGTTGCAGATCCATGCACGTAAACTCGCAAGCCGACTGATGTCTTCTGAACAATGGAAAGGCATTATTGCCGTAAGCCGTGGCGGTCTGGTACCggg                                                                                >  2:102754/1‑133 (MQ=255)                                                             ttCACATGAGCGAAAAATACATCGTCACCTGGGACATGTTGCAGATCCATGCACGTAAACTCGCAAGCCGACTGATGTCTTCTGAACAATGGAAAGGCATTATTGCCGTAAGCCGTGGCGGTCTGGTACCggg                                                                                <  1:102754/133‑1 (MQ=255)                                                             ttCACATGAGCGAAAAATACATCGTCACCTGGGACATGTTGCAGATCCATGCACGTAAACTCGCAAGCCGACTGATGTCTTCTGAACAATGGAAAGGCATTATTGCCGTAAGCCGTGGCGGTCTGGTACCGGGTGCGTTa                                                                         >  1:842021/1‑140 (MQ=255)                                                                  aTGAGCGAAAAATACATCGTCACCTGGGACATGTTGCAGATCCATGCACGTAAACTCGCAAGCCGACTGATGTCTTCTGAACAATGGAAAGGCATTATTGCCGTAAGCCGTGGCGGTCTGGTACCGGGTGCGTTACTGgc                                                                    >  2:562187/1‑140 (MQ=255)                                                                       cGAAAAATACATCGTCACCTGGGACATGTTGCAGATCCATGCACGTAAACTCGCAAGCCGACTGATGTCTTCTGAACAATGGAAAGGCATTATTGCCGTAAGCCGTGGCGGTCTTGTACCGGGTGGGTTACTGGCGCCTg                                                               >  2:215217/1‑140 (MQ=255)                                                                       cGAAAAATACATCGTCACCTGGGACATGTTGCAGATCCATGCACGTAAACTCGCAAGCCGACTGATGTCTTCTGAACAATGGAAAGGCATTATTGCCGTAAGCCGTGGCGGTCTGGTACCGGGTGCGTTACTGGCGCGTg                                                               >  2:433546/1‑140 (MQ=255)                                                                         aaaaaTACATCGTCACCTGGGACATGTTGCAGATCCATGCACGTAAACTCGCAAGCCGACTGATGTCTTCTGAACAATGGAAAGGCATTATTGCCGTAAGCCGTGGCGGTCTGGTACCGGGTGCGTTACTGGCGCGTGaa                                                             <  2:842021/140‑1 (MQ=255)                                                                           aaaTACATCGTCACCTGGGACATGTTGCAGATCCATGCACGTAAACTCGCAAGCCGACTGATGTCTTCTGAACAATGGAAAGGCATTATTGCCGTAAGCCGTGGCGGTCTGGTACCGGGTGCGTTACTGGCGCGTGAACt                                                           <  1:179954/140‑1 (MQ=255)                                                                           aaaTACATCGTCACCTGGGACATGTTGCAGATCCATGCACGTAAACTCGCAAGCCGACTGATGTCTTCTGAACAATGGAAAGGCATTATTGCCGTAAGCCGTGGCGGTCTGGTACCGGGTGCGTTACTGGCGCGTGAACt                                                           <  1:29004/140‑1 (MQ=255)                                                                               aCATCGTCACCTGGGACATGTTGCAGATCCATGCACGTAAACTCGCAAGCCGACTGATGTCTTCTGAACAATGGAAAGGCATTATTGCCGTAAGCCGTGGCGGTCTGGTACCGGGTGCGTTACTGGCGCGTGAACTGGGt                                                       >  1:438681/1‑140 (MQ=255)                                                                                      ccccTGGGACATGTTGCAGATCTATCCACTTAAACTCACAAGCCGACTGATGTCTTCTGAACAATGGAAAGGCATTATTGCCGTAAGCCGTGGCGGTCTGGTACCGGGTGCGTTACTGGCGCGTGAACTGGGTATTCGTc                                                <  1:260859/138‑1 (MQ=255)                                                                                      cACCTGGGACATGTTGCAGATCCATGCACGTAAACTCGCAAGCCGACTGATGTCTTCTGAACAATGGAAAGGCATTATTGCCGTAAGCCGTGGCGGTCTTGTACCGGGTGCGTTACTGGCGCGTGAACCGGGTATTCGtt                                                >  2:858795/1‑139 (MQ=255)                                                                                             gACATGTTGCAGATCCATGCACGTAAACTCGCAAGCCGACTGATGTCTTCTGAACAATGGAAAGGCATTATTGCCGTAAGCCGTGGCGGTCTGGTACCGGGTGCGTTACTGGCGCGTGAACTGGGTATTCGTCATGTCGa                                         >  2:582400/1‑140 (MQ=255)                                                                                             gACATGTTGCAGAGCCATGCACGTAAACTCGCAAGCCGACTGATGTCTCCTGAACAATGGAAAGGCATTATTGCCGTAAGCCGTGGCGGTCTGGTACCGGGTGCGTTACGGGCGCGTGAACTGGGTATTCGTCATGTCGa                                         <  2:719708/140‑1 (MQ=255)                                                                                              aCATGTTGCAGATCCATGCACGTAAACTCGCAAGCCGACTGATGTCTTCTGAACAATGGAAAGGCATTATTGCCGTAAGCCGTGGCGGTCTGGTACCGGGTGCGTTACTGGCGCGTGAACTGGGTATTCGTCATGTCGAt                                        <  1:42586/140‑1 (MQ=255)                                                                                                aTGTTGCAGATCCATGCACGTAAACTCGCAAGCCGACTGATGTCTTCTGAACAATGGAAAGGCATTATTGCCGTAAGCCGTGGCGGTCTGGTACCGGGTGCGTTACTGGCGCGTGAACTGGGTATTCGTCATGTCGATAc                                      >  2:593039/1‑140 (MQ=255)                                                                                                  gTTGCAGATCCATGCACGTAAACTCGCAAGCCGACTGATGTCTTCTGAACAATGGAAAGGCATTATTGCCGTAAGCCGTGGCGGTCTGGTACCGGGTGCGTTACTGGCGCGTGAACTGGGTATTCGTCATGTCGATACCg                                    <  2:606205/140‑1 (MQ=255)                                                                                                          tCCATGCACGTAAACTCGCAAGCCGACTGATGTCTTCTGAACAATGGAAAGGCATTATTGCCGTAAGCCGTGGCGGTCTGGTACCGGGTGCGTTACTGGCGCGTGAACTGGGTATTCGTCATGTCGATACCGTTTGTAtt                            <  1:858795/140‑1 (MQ=255)                                                                                                          tCCATGCACGTAAACTCGCAAGCCGACTGATGTCTTCTGAACAATGGAAAGGCATTATTGCCGTAAGCCGTGGCGGTCTGGTACCGGGTGCATTACTGGCACGTAAACAGGGTATTCATCATTCCGATACCATTTGTAtt                            >  2:418793/1‑140 (MQ=255)                                                                                                           ccATGCACGTAAACTCGCAAGCCGACTGATGTCTTCTGAACAATGGAAAGGCATTATTGCCGTAAGCCGTGGCGGTCTGGTACCGGGTGCGTTACTGGCGCGTGGACTCGGTATTCGACATGAGGATACCGTTTGTAttt                           >  2:380219/1‑140 (MQ=255)                                                                                                           ccATGCACGTAAACTCGCAAGCCGACTGATGTCTTCTGAACAATGGAAAGGCATTATTGCCGTAAGCCGTGGCGGTCTGGTACCGGGTGCGTTACTGGCGCGTGAACTGGGAATTCGTCATGTCGATACCGTTTGTAttt                           >  2:751292/1‑140 (MQ=255)                                                                                                              tGCACGTAAACTCGCAAGCCGACTGATGTCTTCTGAACAATGGAAAGGCATTATTGCCGTAAGCCGTGGCGGTCTGGTACCGGGTGCGTTACTGGCGCGTGAACTGGGTATTCGTCATGTCGATACCGTTTGTATTTCCa                        >  2:198850/1‑140 (MQ=255)                                                                                                              tGCACGTAAACTCGCAAGCCGACTGATGTCTTCTGAACAATGGAAAGGCATTATTGCCGTAAGCCGTGGCGGTCTGGTACCGGGTGCGTTAATGGCGCGCGAACCGCGTATTCGTCATGTCGCCACCGTTTGTATTTCCa                        >  2:392677/1‑140 (MQ=255)                                                                                                                cACGTAAACTCGCAAGCCGACTGATGTCTTCTGAACAATGGAAAGGCATTATTGCCGTAAGCCGTGGCGGTCTGGTACCGGGTGCGTTACTGGCGCGTGAACTGGGTATTCGTCATGTCGAt                                        <  1:509327/122‑1 (MQ=255)                                                                                                                cACGTAAACTCGCAAGCCGACTGATGTCTTCTGAACAATGGAAAGGCATTATTGCCGTAAGCCGTGGCGGTCTGGTACCGGGTGCGTTACTGGCGCGTGAACTGGGTATTCGTCATGTCGAt                                        >  2:509327/1‑122 (MQ=255)                                                                                                                cACGTAAACTCGCAAACCGACTGATATCTTCTGAACACTGGAAAGGCATTATTGCCGTAAGCCGTGGCGGTCTGGTACCGGGTGCGTTACTGGCGCGTGAACTGGGTATTCGTCATGTCGATACCGTTTGTATTTCCAGc                      <  1:380219/140‑1 (MQ=255)                                                                                                                     aaaCTCGCAAGCCGACTGATGTCTTCTGAACAATGGAAAGGCATTATTGCCGTAAGCCGTGGCGGTCTGGTACCGGGTGCGTTACTGGCGCGTGAACTGGGTATTCGTCATGTCGATACCGTTTGTATTTCCAGCTACGa                 >  1:807164/1‑140 (MQ=255)                                                                                                                     aaaCTCGCAAGCCGACTGATGTCTTCTGAACAATGGAAAGGCATTATTGCCGTAAGCCGTGGCGGTCTGGTACCGGGTGCGTTACTGGCGCGTGAACTGGGTATTCGTCATGTCGATACCGTTTGTATTTCCAGCTACGa                 <  1:582400/140‑1 (MQ=255)                                                                                                                     aaaCTCGCAAGCCGACTGATGTCTTCTGAACAATGGAAAGGCATTATTGCCGTAAGCCGTGGCGGTCTGGTACCGGGTGCGTTACTGGCGCGTGAACTGGGTATTCGTCATGTCGATACCGTTTGTATTTCCAGCTACGa                 <  1:751292/140‑1 (MQ=255)                                                                                                                     aaaCTCGCAAGCCGACTGATGTCTTCTGAACAATGGAAAGGCATTATTGCCGTAAGCCGTGGCGGTCTGGTACCGGGTGCGTTACTGGCGCGTGAACTGGGTATTCGTCATGTCGATACCGTTTGTATTTCCAGCTACGa                 <  2:807164/140‑1 (MQ=255)                                                                                                                                 cGACTGATGTCTTCTGAACAATGGAAAGGCATTATTGCCGTAAGCCGTGGCGGTCTGGTACCTGGTGCGTTACTGGCGCGTGAACTGGGTAATCGTCATGCCGAGACCGTTTGTATTCCCAGTTACGATCACGACAACCa     >  2:345450/1‑140 (MQ=255)                                                                                                                                    cTGATGTCTTCTGAAGAATGGAAAGACATAATTGCCGGAAGCCGTGGCGGTCTGGTACCGGGTGCGTTACTGGCGCGTGAACTGGGTATTCGTCATGTCGATACCGTTTGTATTTCCAGCTACGATCACGACAACCAgcg  <  1:392677/140‑1 (MQ=255)                                                                                                                                    cTGATGTCTTCTGAACAGTGGATAGGCATTACTGCAGTACGCCGTGGCAGTCTGGTACCGGGTGCGTTACTGGCGCGTGAACTGGGTATTCGTCATGTCGATACCGTTTGTATTTCCAGCTACGATCACGACAACCAgcg  <  2:235775/140‑1 (MQ=255)                                                                                                                                    cTGATGTCTTCTGAACAATGGAAAGGCATTATTGCCGTAAGCCGTGGCGGTCTGGTACCGGGTGCGTTACTGGCGCGTGAACTGGGTATTCGTCATGTCGATACCGTTTGTATTTCCAGCTACGATCACGACAACCAgcg  <  1:593039/140‑1 (MQ=255)                                                                                                                                    cTGATGTCTTCTGAACAATGGAAAGGCATTATTGCCGTAAGCCGTGGCGGTCTGGTACCGGGTGCGTTACTGGCGCGTGAACTGGGTATTCGTCATGTCGATACCGTTTGTATTTCCAGCTACGATCACGACAACCAgcg  <  1:198850/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                      TCGCGCAACCTATTTTCCCCTCGAACACTTTTTAAGCCGTAGATAAACAGGCTGGGACACTTCACATGAGCGAAAAATACATCGTCACCTGGGACATGTTGCAGATCCATGCACGTAAACTCGCAAGCCGACTGATGCCTTCTGAACAATGGAAAGGCATTATTGCCGTAAGCCGTGGCGGTCTGGTACCGGGTGCGTTACTGGCGCGTGAACTGGGTATTCGTCATGTCGATACCGTTTGTATTTCCAGCTACGATCACGACAACCAGCG  >  NC_000913/255912‑256182

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑