Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,206,448	A→G	M252V (ATG→GTG)	yehM →	putative YehN

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,206,448	0	A	G	98.0%	161.4 / NA	49	M252V (ATG→GTG)	yehM	putative YehN
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (26/22);  minor base T (1/0);  total (27/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGCAAAGATGTGGCGCGAATGCCCTCAGAAAATTAACAAGCCAGAATTGCCCTCGCTGGCAGATGCCGTTACAGGTTGTTATCTCACACCCTACAGTGAAAAGCGCCTTGATGTGCTGGCAGGATACCTTTCAGGAATGCCTGCCCCGGTATGGCAAAACTGGTGCTGGCAGTGGGGCTTGCAGAAGGCCGGTGAACAACTGCTAAAAACTATCCTTACCCGTTTGCGCCAGCACAAATTGCCCGCTTCTACCGCGGATATGGCTGCCGCTCAT  >  NC_000913/2206312‑2206585                                                                                                                                         |                                                                                                                                          ggCAAAGATGTGGCGCGAATGCCCTCAGAAAATTAACAAGCCAGAATTGCCCTCGCTGGCAGATGCCGTTACAGGTTGTTATCTCACACCCTACAGTGAAAAGCGCCTTGATGTGCTGGCAGGATACCTTTCAGGAGtgc                                                                                                                                        <  2:545233/140‑1 (MQ=255)  gCAAAGATGTGGCGCGAATGCCCTCAGAAAATTAACAAGCGAGAATTGCCCTCGCTGGCAGATGCCGTTACAGGTTGTTATCTCACACCCTACAGTGAAAAGCGGCTTTATGTGCTGTCAGGATACCTTTCAGGAGtgcc                                                                                                                                       >  2:373177/1‑140 (MQ=255)  gCAAAGATGTGGCGCGAATGCCCTCAGAAAATTAACAAGCCAGAATTGCCCTCGCTGGCAGATGCCGTTACAGGTTGTTATCTCACACCCTACAGTGAAAAGCGCCTTGATGTGCTGGCAGGATACCTTTCAGGAGtgcc                                                                                                                                       <  2:522051/140‑1 (MQ=255)  gCAAAGATGTGGCGCGAATGCCCTCAGAAAATTAACAAGCCAGAATTGCCCTCGCTGGCAGATGCCGTTACAGGTTGTTATCTCACACCCTACAGTGAAAAGCGCCTTGATGTGCTGGCAGGATACCTTTCAGGAGtgcc                                                                                                                                       <  2:103074/140‑1 (MQ=255)  gCAAAGATGTGGCGCGAATGCCCTCAGAAAATTAACAAGCCAGAATTGCCCTCGCTGGCAGATGCCGTTACAGGTTGTTATCTCACACCCTACAGTGAAAAGCGCCTTGATGTGCT‑GCAGGATACCTTTCAGGAGtgcct                                                                                                                                      >  1:636275/1‑140 (MQ=255)           tgGCGCGAATGCCCTCAGAAAATTAACAAGCCAGAATTGCCCTCGCTGGCAGATGCCGTTACAGGTTGTTATCTCACACCCTACAGTGAAAAGCGCCTTGATGTGCTGGCAGGATACCTTTCAGGAGTGCCTGCCCCGGt                                                                                                                              <  2:619553/140‑1 (MQ=255)            ggCGCGAATGCCCTCAGAAAATTAACAAGCCAGAATTGCCCTCGCTGGCAGATGCCGTTACAGGTTGTTATCTCACACCCTACAGTGAAAAGCGCCTTGATGTGCTGGCAGGATACCTTTCAGGAGTGCCTGCCCCGGTa                                                                                                                             >  1:13728/1‑140 (MQ=255)            ggCGCGAATGCCCTCAGAAAATTAACAAGCCAGAATTGCCCTCGCTGGCAGATGCCGTTACAGGTTGTTATCTCACACCCTACAGTGAAAAGCGCCTTGATGTGCTGGCAGGATACCTTTCAGGAGTGCCTGCCCCGGTa                                                                                                                             >  1:471288/1‑140 (MQ=255)            ggCGCGAATGCCCTCAGAAAATTAACAAGCCAGAATTGCCCTCGCTGGCAGATGCCGTTACAGGTTGTTATCTCACACCCTACAGTGAAAAGCGCCTTGATGTGCTGGCAAGATGCCATTCAGGCGTGCCTGCCCCGGTa                                                                                                                             >  1:410211/1‑140 (MQ=255)            ggCGCGAATGCCCTCAGAAAATTAACAAGCCAGAATTGCCCTCGCT‑GCAGATGCCGTTACAGGTTGTTATCTCACACCCTACAGTGAAAAGCGCCTTGATGTGCTGGCAGGATACCTTTCAGGATTGCCTGCCCCGGTAt                                                                                                                            >  1:288777/1‑140 (MQ=255)                         tCAGAAAATTAACAAGCCAGAATTGCCCTCGCTGGCAGATGCCGTTACAGGTTGTTATCTCACACCCTACAGTGAAAAGCGCCTTGATGTGCTGGCAGGATACCTTTCAGGAGTGCCTGCCCCGGTATGGCAAAACTGGt                                                                                                                <  2:434141/140‑1 (MQ=255)                           aGAAAATTAACAAGCCAGAATTGCCCTCGCTGGCAGATGCCGTTACAGGTTGTTATCTCACACCCTACAGTGAAAAGCGCCTTGATGTGCTGGCAGGATACCTTTCAGGAGTGCCTGCCCCGGTATGGCAAAACTGGTGc                                                                                                              <  2:567704/140‑1 (MQ=255)                               aaTTAACAAGCCAGAATTGCCCTCGCTGGCAGATGCCGTTACAGGTTGTTATCTCACACCCTACAGTGAAAAGCGCCTTGATGTGCTGGCAGGATACCTTTCAGGAGTGCCTGCCCCGGTATGGCAAAACTGGTGCTGGc                                                                                                          >  2:838102/1‑140 (MQ=255)                                aTTAACAAGCCAGAATTGCCCTCGCTGGCAGATGCCGTTACAGGTTGTTATCTCACACCCTACAGTGAAAAGCGCCTTGATGTGCTGGCAGGATACCTTTCAGGAGTGCCTGCCCCGGTATGGCAAAACTGGTGc                                                                                                              <  2:55645/135‑1 (MQ=255)                                aTTAACAAGCCAGAATTGCCCTCGCTGGCAGATGCCGTTACAGGTTGTTATCTCACACCCTACAGTGAAAAGCGCCTTGATGTGCTGGCAGGATACCTTTCAGGAGTGCCTGCCCCGGTATGGCAAAACTGGTGc                                                                                                              >  1:55645/1‑135 (MQ=255)                                  tAACAAGCCAGAATTGCCCTCGCTGGCAGATGCCGTTACAGGTTGTTATCTCACACCCTACAGTGAAAAGCGCCTTGATGTGCTGGCAGGATACCTTTCAGGTGTGCCTGACCCAGTATGGCAATACTGGTGCTGGCAGt                                                                                                       >  1:361219/1‑140 (MQ=255)                                  tAACAAGCCAGAATTGCCCTCGCTGGCAGATGCCGTTACAGGTTGTTATCTCACACCCTACAGTGAAAAGCGCCTTGATGTGCTGGCAGGATACCTTTCAGGGGTGCCTGCCCCGGTGTGGCAACACTGGTGCTGGCAGt                                                                                                       >  1:287129/1‑140 (MQ=255)                                         ccAGAATTGCCCTCGCTGGCAGATGCCGTTACAGGTTGTTATCTCACACCCTACAGTGAAAAGCGCCTTGATGTGCTGGCAGGATACCTTTCAGGAGTGCCTGCCCCGGTATGGCAAAACTGGTGCTGGCAGTGGGGCtt                                                                                                >  1:715944/1‑140 (MQ=255)                                                  cccTCGCTGGCAGATGCCGTTACAGGTTGTTATCTCACACCCTACAGTGAAAAGCGCCTTGATGTGCTGGCAGGATACCTTTCAGGAGTGCCTGCCCCGGTATGGCAAAACTGGTGCTGGCAGTGGGGCTTGCAGAAGGc                                                                                       >  1:202054/1‑140 (MQ=255)                                                  cccTCGCTGGCAGATGCCGTTACAGGTTGTTATCTCACACCCTACAGTGAAAAGCGCCTTGATGTGCTGGCAGGATACCTTTCAGGAGTGCCTGCCCCGGTATGGCAAAACTGGTGCTGGCAGTGGGGCTTGCAGAAGGc                                                                                       >  2:713724/1‑140 (MQ=255)                                                       gCTGGCAGATGCCGTTACAGGTTGTTATCTCACACCCTACAGTGAAAAGCGCCTTGATGTGCTGGTAGGATACCTTTCAGGAGTGCCTGCCCCGGTATGGCAAAACTGGTGCTGGCAGTGGGGCt                                                                                                 <  1:191755/125‑1 (MQ=255)                                                       gCTGGCAGATGCCGTTACAGGTTGTTATCTCACACCCTACAGTGAAAAGCGCCTTGATGTGCTGGTAGGATACCTTTCAGGAGTGCCTGCCCCGGTATGGCAAAACTGGTGCTGGCAGTGGGGCt                                                                                                 >  2:191755/1‑125 (MQ=255)                                                            cAGATGCCGTTACAGGTTGTTATCTCACACCCTACAGTGAAAAGCGCCTTGATGTGCTGGCAGGATACCTTTCAGGAGTGCCTGCCCCGGTATGGCAAAACTGGTGCTGGCAGTGGGGCTTGCAGAAGGCCGGTGaacaa                                                                             <  2:13728/140‑1 (MQ=255)                                                                 gCCGTTACAGGTTGTTATCTCACACCCTACAGTGAAAAGCGCCTTGATGTGCTGGCAGGATACCTTTCAGGAGTGCCTGCCCCGGTATGGCAAAACTGGTGCTGGCAGTGGGGCTTGCAGAAGGCCGGTGAACAACTGCt                                                                        <  1:838102/140‑1 (MQ=255)                                                                              gttATCTCACACCCTACAGTGAAAAGCGCCTTGATGTGCTGGCAGGATACCTTTCAGGAGTGCCTGCCCCGGTATGGCAAAACTGGTGCTGGCAGTGGGGCTTGCAGAAGGCCGGTGAACAACTGCTAAAAACTATCCtt                                                           >  1:639104/1‑140 (MQ=255)                                                                              gttATCTCACACCCTACAGTGAAAAGCGCCTTGATGTGCTGGCAGGATACCTTTCAGGAGTGCCTGCCCCGGTATGGCAAAACTGGTGCTGGCAGTGGGGCTAGCAGAAGGCCGCTGAACAACTTCTAAAAACTATCCtt                                                           >  1:372487/1‑140 (MQ=255)                                                                               ttATCTCACACCCTATAGTGAACAGTGCCGTGATGTGCTGGCAGGATACCTTTCAGGAGTGCCTGCCCCGGTATGGCAAAACTGGTGCTGGCAGTGGGGCTTGCAGAAGGCCGGTGAACAACTGCTAAAAACTATCCTTa                                                          <  1:248050/140‑1 (MQ=255)                                                                                     agacagcTACAGTGATAAGCGCGTTAATGGGCTGGCACGATCCCTTTCAGGAGTGCCTGCCCCGGTATGGCAAAACTGGTGCTGGCAGTGGGGCTTGCAGAAGGCCGGTGAACAACTGCTAAAAACTATCCTTACCCGtt                                                    <  1:332244/134‑1 (MQ=255)                                                                                         cccTACAGTGAAAAGCGCCTTGTTGTGCTGGTAGGATACATTTCAGGAGTGCCTGCCCCGGTATGGCAAAACTGGTGCTGGCAGTGGGGCTTGCAGAAGGCCGGTGAACAACTGCTAAAAACTATCCTTACCCGTTTgcg                                                <  2:410211/140‑1 (MQ=255)                                                                                         cccTACAGTGAAAAGCGCCTTGATGTGCTGGCAGGATACCTTTCAGGAGTGCCTGCCCCGGTATGGCAACACTGGTGCTGGCAGTGGGGCTTGCAGAAGGCCGGTGAACAACTGCTAAAAACTATCCTTACCCGTTTgcg                                                <  2:715944/140‑1 (MQ=255)                                                                                          ccTACAGTGAAAAGCGCCTTGATGTGCTGGCAGGATACCTTTCAGGAGTGCCTGCCCCGGTATGGCAAAACTGGTGCTGGCAGTGGGGCTTGCAGAAGGCCGGTGAACAACTGCTAAAAACTATCCTTACCCGTTTgcgc                                               >  2:449686/1‑140 (MQ=255)                                                                                            tACAGTGAAAAGCGCCTTGATGTGCTGGCAGGATACCTTTCAGGAGTGCCTGCCCCGGTATGGCAAAACTGGTGCTGGCAGTGGGGCTTGCGGAAGGCCGGTGAGCAAGTGCTAAAACCTATTCTTACCCGTTTGCGCCa                                             >  2:225535/1‑140 (MQ=255)                                                                                            tACAGTGAAAAGCGCCTTGATGTGCTGGCAGGATACCTTTCAGGAGTGCCTGCCCCGGTATGGCAAAACTGGTGCTGGCAGTGGGGCTTGCAGAAGGCCGGTGAACAACTGCTAAAAACTATCCTTACCCGTTTGCGCCa                                             >  2:773729/1‑140 (MQ=255)                                                                                                  gAAAAGCGCCTTGATGTGCTGGCAGGATACCTTTCAGGAGTGCCTGCCCCGGTATGGCAAAACTGGTGCTGGCAGTGGGGCTTGCAGAAGGCCGGTGAACAACTGCTAAAAACTATCCTTACCCGTTTGCGCCAGCACaa                                       >  1:16476/1‑140 (MQ=255)                                                                                                   aaaaGCGCCTTGATGTGTTGGCTGGATACCTCTCATGAGGGCCTGCCCCGGTATGGCAAAACTGGTGCTGGCAGTGGGGCTTGCAGAAGGCCGGTGAACAACTGCTAAAAACTATCCTTACCCGTTTGCGCCAGCACaaa                                      <  2:287129/140‑1 (MQ=255)                                                                                                   aaaaGCGCCTTGATGTGCTGGCAGGATACCTCTCACGAGAGCCTGCCCCGGTATGGCAAAACTGGTGCTGGCAGTGGGGCTTGCAGAAGGCCGGTGAACAACTGCTAAAAACTATCCTTACCCGTTTGCGCCAGCACaaa                                      <  2:361219/140‑1 (MQ=255)                                                                                                    aaaGCGCCTTGATGTGCTGGCAGGATACCTTTCAGGAGTGCCTGCCCCGGTATGGCAAAACTGGTGCTGGCAGTGGGGCTTGCAGAAGGCCGGTGAACAACTGCTAAAAACTATCCTTACCCGTTTGCGCCAGCACAAAt                                     >  1:29154/1‑140 (MQ=255)                                                                                                    aaaGCGCCTTGATGTGCTGGCAGGATACCTTTCAGGAGTGCCTGCCCCGGTATGGCAAAACTGGTGCTGGCAGTGGGGCTTGCAGAAGGCCGGTGAACAACTACTAAAAAGTATACTGACCCGTGTGCGCCAGCACAAAt                                     >  1:239552/1‑140 (MQ=255)                                                                                                      aGCGCCTTGATGTGCTGGCAGGATACCTTTCAGGAGTGCCTGCCCCGGTATGGCAAAACTGGTGCTGGCAGTGGGGCTTGCAGAAGGCCGGTGAGCAACTGCTAAAAACTATCCTTACCCGTTTGCGCCAGCACAAATTg                                   >  1:550515/1‑140 (MQ=255)                                                                                                      aGCGCCTTGATGTGCTGGCAGGATACCTTTCAGGAGTGCCTGCCCCGGTATGGCAAAACTGGTGCTGGCAGTGGGGCTTGCAGAAGGCCGGTGAACAACTGCTAAAAACTATCCTTACCCGTTTGCGCCAGCACAAATTg                                   <  2:16476/140‑1 (MQ=255)                                                                                                      aGCGCCTTGATGTGCTGGCAGGATACCTTTCAGGAGTGCCTGCCCCGGTATGGCAAAACTGGTGCTGGCAGTGGGGCTTGCAGAAGGCCGGAGAACAACTTCTAGAAATTATCCTTAACCGTTTGCGCCAGcacctgtct                                   >  2:332244/1‑134 (MQ=255)                                                                                                      aGCGCCTTGATGTGCTGGCAGGATACCTTTCAGGAGTGCCTGCCCCGGTATGACAAAACTGGTGCTGGCAGTGGGGCTTGCAGAAGGCCGGTGAACAACTGCTAAAAACTATCCTTACCCGTTTGCGCCAGCACAAATTg                                   >  2:675829/1‑140 (MQ=255)                                                                                                        cgcCTTGATGTGCTGGCAGGATACCTTTCAGGAGTGCCTGCCCCGGTATGGCAAAACTGGTGCTGGCAGTGGGGCTTGCAGAAGGCCGGTGAACAACTGCTAAAAACTATCC‑TACCCGTTTGCGCCAGCACAAATTGccc                                >  1:574706/1‑140 (MQ=255)                                                                                                           cTTGATGTGCTGGCAGGATACCTTTCAGGAGTGCCTGCCCCGGTATGGCAAAACTGGTGCTGGCAGTGGGGCTTGCAGAAGGCCGGTGAACCACTGCTAAAAACAATCATTACCCGTTTGCGGCAGCACAAATTGCCCGc                              >  2:294339/1‑140 (MQ=255)                                                                                                           cTTGATGTGCTGGCAGGATACCTTTCAGGAGTGCCTGCCCCGGTATGGCAAAACTGGTGCTGGCAGTGGGGCTTGCAGAAGGCCGGCGAACAACTGCTAAGAACAATCTTTACCCGTGTGCGCCAGCACAAATTGCCCGc                              >  2:327929/1‑140 (MQ=255)                                                                                                                  tgCTGGCAGGATACCCTTCAGGTGTTCCTTCCCCGGTCTGGCAAAACTGGTGCTGGCAGTGGGGCTTGCAGAAGGCCGGTGAACAACTGCTAAAAACTATCCTTACCCGTTTGCGCCAGCACAAATTGCCCGCTTCTAcc                       <  1:225535/140‑1 (MQ=255)                                                                                                                             tcccTTTCAGGAGTGCCTGCCCTGGTATGGCTAAACTGGTGCTGGCAGGGGGGCTTGCAGAAGGCCGGTGAACAACTGCTAAAAACTATCCTTACCCGTTTGCGCCAGCACAAATTGCCCGCTTCTACCGCGGATATGGc            <  2:288777/138‑1 (MQ=255)                                                                                                                                    cAGGAGTGCCTGCCCCGGTATGGCAAAACTGGTGCTGGCAGTGGGGCTTGCAGAAGGCCGGTGAACAACTGCTAAAAACTATCCTTACCCGTTTGCGCCAGCACAAATTGCCCGCTTCTACCGCGGATATGGCTGCCGCt     <  2:476521/140‑1 (MQ=255)                                                                                                                                     aGGAGTGCCTGCCCCGGTATGGCAAAACTGGTGCTGGCAGTGGGGCTTGCAGAAGGCCGGTGAACAACTGCTAAAAACTATCCTTACCCGTTTGCGCCAGCACAAATTGCCCGCTTCTACCGCGGATATGGCTGc         <  2:1284/135‑1 (MQ=255)                                                                                                                                     aGGAGTGCCTGCCCCGGTATGGCAAAACTGGTGCTGGCAGTGGGGCTTGCAGAAGGCCGGTGAACAACTGCTAAAAACTATCCTTACCCGTTTGCGCCAGCACAAATTGCCCGCTTCTACCGCGGATATGGCTGc         >  1:1284/1‑135 (MQ=255)                                                                                                                                      ggAGTGCCTGCCCCGGTATGGCAAAACTGGTGCTGGCAGTGGGGCTTGCAGAAGGCCGGTGAACAACTGCTAAAAACTATCC‑TACCCGTTTGCGCCAGCACAAATTGCCCGCTTCTACCGCGGATATGGCTGCCGCTCAt  <  2:574706/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                          GGCAAAGATGTGGCGCGAATGCCCTCAGAAAATTAACAAGCCAGAATTGCCCTCGCTGGCAGATGCCGTTACAGGTTGTTATCTCACACCCTACAGTGAAAAGCGCCTTGATGTGCTGGCAGGATACCTTTCAGGAATGCCTGCCCCGGTATGGCAAAACTGGTGCTGGCAGTGGGGCTTGCAGAAGGCCGGTGAACAACTGCTAAAAACTATCCTTACCCGTTTGCGCCAGCACAAATTGCCCGCTTCTACCGCGGATATGGCTGCCGCTCAT  >  NC_000913/2206312‑2206585

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑