| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,224,365 | A→G | V169A (GTG→GCG) | pbpG ← | peptidoglycan DD‑endopeptidase PbpG |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,224,365 | 0 | A | G | 98.2% | 179.6 / NA | 54 | V169A (GTG→GCG) | pbpG | peptidoglycan DD‑endopeptidase PbpG |
| Reads supporting (aligned to +/- strand): ref base A (1/0); new base G (24/29); total (25/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.63e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TGCGGAACGGCAGCGTATACGTCGGATTAGAGAAGGTTGCCATATCTTCCCGGGTAGTACTTAACTGCCCGATCAACGGATATTGTTTGCTGGCAATGAGCAGTTTGGTTAAGTCACGGGCAGTTGAAACGTTATGCACCGACAATCCGGTAGGTTCAACAAAGCGCGTGTTGTTCATTCCGAGCGATTTCGCTTTCGCATTCATTGCCTTAATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTG > NC_000913/2224228‑2224500 | tGCGGAACGGCAGCGTATACGTCGGATTAGAGAAGGTTGCCATATCTTCCCGGGTAGTACTTAACTGCCCGATCAACGGATATTGTTTGCTGGCAATGAGCAGTTTGGTTAAGTCACGGGCAGTTGAAACGTTATGCGcc > 1:672577/1‑140 (MQ=255) gCGGAACGGCAGCGTATACGTCGGATTAGAGAAGGTTGCCATATCTTCCCGGGTAGTACTTAACTGCCCGATCAACGGATATTGTTTGCTGGCAATGAGCAGTTTGGTTAAGTCACGGGCAGTTGAAACGTTATGCGCCg > 2:669182/1‑140 (MQ=255) cGGAACGGCAGCGTATACGTCGGATTAGAGAAGGTTGCCATATCTTCCCGGGTAGTACTTAACTGCCCGATCAACGGATATTGTTTGCTGGCAATGAGCAGTTTGGTTAAGTCACGGGCAGTTGAAACGTTATGCGCCGa < 1:818210/140‑1 (MQ=255) cGGAACGGCAGCGTATACGTCGGATTAGAGAAGGTTGCCATATCTTCCCGGGTAGTACTTAACTGCCCGATCAACGGATATTGTTTGCTGGCAATGAGCAGTTTGGTTAAGTCACGGGCAGTTGAAACGTTATGCGCCGa > 1:84952/1‑140 (MQ=255) cGGAACGGCAGCGTATACGTCGGATTAGAGAAGGTTGCCATATCTTCCCGGGTAGTACTTAACTGCCCGATCAACGGATATTGTTTGCTGGCAATGAGCAGTTTGGTTAAGTCACGGGCAGTTGAAACGTTATGCGCCGa > 2:118585/1‑140 (MQ=255) cGGCAGCGTATACGTCGGATTAGAGAAGGTTGCCATATCTTCCCGGGTAGTACTTAACTGCCCGATCAACGGATATTGTTTGCTGGCAATGAGCAGTTTGGTTAAGTCACGGGCAGTTGAAACGTTATGCGCCGACAATc > 2:177479/1‑140 (MQ=255) gCAGCGTATACGTCGGATTAGAGAAGGTTGCCATATCTTCCCGGGTAGTACTTAACTGCCCGATCAACGGATATTGTTTGCTGGCAATGAGCAGTTTGGTTAAGTCACGGGCAGTTGAAACGTTATGCGCCGACAATCCg < 1:493449/140‑1 (MQ=255) gCAGCGTATACGTCGGATTAGAGAAAGTTGCCATATCGTCCCGGGTAGTACTTAACTGCCCGATCAACGGATATTGTTTGCTGGCAATGAGCAGTTTGGTTAAGTCACGGGCAGTTGAAACGTTATGCGCCGACAATCCg < 1:346802/140‑1 (MQ=255) cAGCGTATACGTCGGATTAGAGAAGGTTGCCATATCTTCCCGGGTAGTACTTAACTGCCCGATCAACGGATATTGTTTGCTGGCAATGAGCAGTTTGGTTAAGTCACGGGCAGTTGAAACGTTATGCGCCGACAATCCgg > 1:783374/1‑140 (MQ=255) gacctataCGTCGGATTTGAGATGGTTGCCATATCTTCCCGGGTAGTAATTATCAGCCCGATCAACGGATATTGTTTGCTGGCAATGAGCAGTTTGGTTAAGTCACGGGCAGTTGAAACGTTATGCGCCGACAATCCGGt < 2:312144/136‑1 (MQ=255) aGCGTATACGTCGGATTAGAGAAGGTTGCCATATCTTCCCGGGTAGTACTTAACTGCCCGATCAACGGATATTGTTTGCTGGCAATGAGCAGTTTGGTTAAGTCACGGGCAGTTGAAACGTTATGCGCCGACAATCCGGt < 2:66242/140‑1 (MQ=255) aGCGTATACGTCGGATTAGAGAAGGTTGCCATATCTTCCCGGGTAGTACTTAACTGCCCGATCAACGGATATTGTTTGCTGGCAATGAGCAGTTTGGTTAAGTCACGGGCAGTTGAAACGTTATGCGCCGACAATCCGGt < 2:672577/140‑1 (MQ=255) aGCGTATACGTCGGACTAGAGATGGCTGCCATATCTTCCCGGGTAGTACTTAACTGCCCGATCAACGGATATTGTTTGCTGGCAATGAGCAGTTTGGTTAAGTCACGGGCAGTTGAAACGTTATGCGCCGACAATCCGGt < 1:272683/140‑1 (MQ=255) cGTCGGATTAGAGAAGCGTGCCCTATCTTCGAGTGAAGTCCTTAACTGACCGATCAACGGATATTGTTTGCTGGCAATGAGCAGTTTGGTTAAGTCACGGGCAGTTGAAACGTTATGCGCCGACAATCCGGTAGGTTcaa < 2:419561/140‑1 (MQ=255) gTCGGATTAGAGAAGGTTGCCATATCTTCCCGGGTAGTACTTAACTGCCCGATCAACGGATATTGTTTGCTGGCAATGAGCAGTTTGGTTAAGTCACGGGCAGTTGAAACGTTATGCGCCGACAATCCGGTAGGTTcaac < 1:758328/140‑1 (MQ=255) cGGATTAGAGAAGGTTGCCATATCTTCCCGGGTAGTACTTAACTGCCCGATCAACGGATATTGTTTGCTGGCAATGAGCAGTTTGGTTAAGTCACGGGCAGTTGAAACGTTATGCGCCGACAATCCg < 2:199250/127‑1 (MQ=255) cGGATTAGAGAAGGTTGCCATATCTTCCCGGGTAGTACTTAACTGCCCGATCAACGGATATTGTTTGCTGGCAATGAGCAGTTTGGTTAAGTCACGGGCAGTTGAAACGTTATGCGCCGACAATCCg > 1:199250/1‑127 (MQ=255) aTTAGAGAAGGTTGCCATATCTTCCCGGGTAGTACTTAACTGCCCGATCAACGGATATTGTTTGCTGGCAATGAGCAGTTTGGTTAAGTCACGGGCAGTTGAAACGTTATGCGCCGACAATCCGGTAGGTTCAACAAAgc < 1:75192/140‑1 (MQ=255) aTTAGAGAAGGTTGCCATATCTTCCCGGGTAGTACTTAACTGCCCGATCAACGGATATTGTTTGCTGGCAATGAGCAGTTTGGTTAAGTCACGGGCAGTTGAAACGTTATGCGCCGACAATCCGGTAGGTTCAACAAAgc < 1:177479/140‑1 (MQ=255) aagaAGGTTGCCATATCTTCCCGGGTAGTTCTTAACTGCCCGATCAACGGATATTGTTTGCTGGCAATGAGCAGTTTGGTTAAGTCACGGGCAGTTGAAACGTTATGCGCCGACAATCCGGTAGGTTCAACAAAGCGCgt < 2:197441/139‑1 (MQ=255) agaAGGTTGCCATATCTTCCCGGGTAGTACTTAACTGCCCGATCAACGGATATTGTTTGCTGGCAATGAGCAGTTTGGTTAAGTCACGGGCAGTTGAAACGTTATGCGCCGACAATCCGGTAGGTTCAACAAAGCGCgtg > 2:848618/1‑140 (MQ=255) aaGGTTGCCATATCTTCCCGGGTAGTACTTAACTGCCCGATCAACGGATATTGTTTGCTGGCAATGAGCAGTTTGGTTAAGTCACGGGCAGGTGAAACGTGATGCGCCAACAATCCGGTAGGGTCAACAAAGCGCGtgtt > 2:218554/1‑140 (MQ=255) gTTGCCATATCTTCCCGGGTAGTACTTAACTGCCCGATCAACGGATATTGTTTGCTGGCAATGAGCAGTTTGGTTAAGTCACGGGCAGTTGAAACGTTATGCACCGACAAACCGCTAGGTTCAAGAAAGCGCGTgttgtt > 1:328384/1‑140 (MQ=255) ccATATCTTCCCGGGTAGTACTTAACTGCCCGATCAACGGATATTGTTTGCTGGCAATGAGCAGTTTGGTTAAGTCACGGGCAGTTGAAACGTTATGCGCCGACAATCCGGTAGGTTCAACAAAGCGCGTGTTGTTCAtt < 1:547462/140‑1 (MQ=255) tCTTCCCGGGTAGTACTTAACTGCCCGATCAACGGATATTGTTTGCTGGCAATGAGCAGTTTGGTTAAGTCACGGGCAGTTGAAACGTTATGCGCCGACAATCCGGTAGGGTCAGCAAAGCGCGAGTTGTTCATTCCGAg > 1:313667/1‑140 (MQ=255) ttCCCGGGTAGTACTTAACTGCCCGATCAACGGATATTGTTTGCTGGCAATGAGCAGTTTGGTTAAGTCACGGGCAGTTGAAACGTTATGCGCCGACAATCCGGTAGGTTGAACGAAGCGCGTGGTGTTCATTCCGAGCg > 1:242792/1‑140 (MQ=255) tCCCGGGTAGTACTTAACTGCCCGATCAACGGATATTGTTTGCTGGCAATGAGCAGTTTGGTTAAGTCACGGGCAGTTGAAACGTTATGCGCCGACAATCCGGTAGGTTCAACAAAGCGCGTGTTGTTCATTCCGAGCGa > 1:829072/1‑140 (MQ=255) ggTAGTACTTAACTGCCCGATCAACGGATATTGTTTGCTGGCAATGAGCAGTTTGGTTAAGTCACGGGCAGTTGAAACGTTATGCGCCGACAATCCGGTAGGTTCAACAAAGCGCGTGTTGTTCATTCCGAGCGAtttcg < 1:848618/140‑1 (MQ=255) tagtaCTTAACTGCCCGATCAACGGATATTGTTTGCTGGCAATGAGCAGTTTGGTTAAGTCACGGGCAGTTGAAACGTTATGCGCCGACAATCCGGTAGGTTCAACAAAGCGCGTGTTGTTCATTCCGAGCGAtttcgct > 1:84930/1‑140 (MQ=255) taCTTAACTGCCCGATCAACGGATATTGTTTGCTGGCAATGAGCAGTTTGGTTAAGTCACGGGCAGTTGAAACGTTATGCGCCGACAATCCGGTAGGTTCAACAAAGCGCGAGTTGTTCATTCCGAGCGAtttcgctttc > 1:208830/1‑140 (MQ=255) ttAACTGCCCGATCAACGGATATTGTTTGCTGGCAATGAGCAGTTTGGTTAAGTCACGGGCAGTTGAAACGTTATGCGCCGACAATCCGGTAGGTTCAACAAAGCGCGTGTTGTTCATTCCGAGCCATTTCGCTTTCGca < 2:533278/140‑1 (MQ=255) cccGATCAACGGATATTGTTTGCTGGCAATGAGCAGTTTGGTTAAGTCACGGGCAGTTGAAACGTTATGCGCCGACAATCCGGTAGGTTCAACAAAGCGCGTGTTGTTCATTCCGAGCGATTTCGCTTTCGCATTCATTg > 1:208349/1‑140 (MQ=255) cGATCAACGGATATTGTTTGCTGGCAATGAGCAGTTTGGTTAAGTCACGGGCAGTTGAAACGTTATGCGCCGACAATCCGGTAGGTTCAACAAAGCGCGTGTTGTTCATTCCGAGCGATTTCGCTTTCGCATTCATTGcc < 2:95646/140‑1 (MQ=255) cGATCAACGGATATTGTTTGCTGGCAATGAGCAGTTTGGTTAAGTCACGGGCAGTTGAAACGTTATGCGCCGACAATCCGGTAGGTTCAACAAAGCGCGTGTTGTTCATTCCGAGCGATTTCGCTTTCGCATTCATTGcc < 1:541025/140‑1 (MQ=255) cGATCAACGGATATTGTTTGCTGGCAATGAGCAGTTTGGTTAAGTCACGGGCAGTTGAAACGTTATGCGCCGACAATCCGGTAGGTTCAACAAAGCGCGTGTTGTTCATTCCGAGCGATTTCGCTTTCGCATTCATTGcc < 2:84952/140‑1 (MQ=255) cGATCAACGGATATTGTTTGCTGGCAATGAGCAGTTTGGTTAAGTCACGGGCAGTTGAAACGTTATGCGCCGACAATCCGGTAGGTTCAACAAAGCGCGAGTTGTTCATTCCGAGCGATTTCGCTTTCGCATTCATTGcc < 2:208830/140‑1 (MQ=255) cGATCAACGGATATTGTTTGCTCGCAATGAGCAGTCTGGCTAAGTCACCGGCAGTTGAAACGTTATGCGCCGACAATCCGGTAGGTTCAACAAAGCGCGTGTTGTTCATTCCGAGCGATTTCGCTTTCGCATTCATTGcc < 2:242792/140‑1 (MQ=255) aaCGGATATTGTTTGCTGGCAATGAGCAGTTTGGTTAAGTCACGGGCAGTTGAAACGTTATGCGCCGACAATCCGGTAGGTTCAACAAAGCGCGTGTTGTTCATTCCGAGCGATTTCGCTTTCGCATTCATTGCCTtaat < 2:14180/140‑1 (MQ=255) gATATTGTTTGCTGGCACTGAGAAGTTTGGTAAAGGCACAGGCAGTTGCAACGTTATGCGCCGACAATCCGGTAGGTTCAACAAAGAGCGTGTTGTTCATTCCGAGCGATTTCGCTTTCGCATTCATTGCTGTAATAAAg < 2:295284/140‑1 (MQ=255) ttGCTGGCAATGAGCAGTTTGGTTAAGTCACGGGCAGTTGAAACGTTATGCGCCGACAATCCGGTAGGTTCAACAAAGCGCGTGTTGTTCATTCCGAGCGATTTCGCTTTCGCATTCATTGCCTTAATAAAGGCTTTGTa < 1:669182/140‑1 (MQ=255) cTGGCAATGAGCAGTTTGGTTAAGTCACGGGCAGTTGAAACGTTATGCGCCGACAATCCGGTAGGTTCAACAAAGCGCGTGTTGTTCATTCCGAGCGATTACGCCTTCACATTCATTGCCTTCATAAAGGCTTTGTAacc > 2:315523/1‑140 (MQ=255) ggCAATGAGCAGTTTGGTTAAGTCACGGGCAATTGTAACCTTATGCGCTGACAATCCGGTAGGTTCAACAAAGCGCGTGTTGTTCATTCCGAGCGATTTCGCTTTCGCATTCATTGCCTTAATAAAGGCTTTGTAaccac < 2:328384/140‑1 (MQ=255) ggCAATGAGCAGTTTGGTTAAGACACGGGCATTTGGAACGTTATGCGCAGACAATCCGGTAGGTTCAACAAAGCGCGTGTTGTTCATTCCGAGCGATTTCGCTTTCGCATTCATTGCCTTAATAAAGGCTTTGTAaccac < 2:313667/140‑1 (MQ=255) ggCAATGAGCAGTTTAGTTAAGCCACGGGAAGTTGAAACGTTATGCGCCGACAATCCGGTAGGTTCAACAAAGCGCGTGTTGTTCATTCCGAGCGATTTCGCTTTCGCATTCATTGCCTTAATAAAGGCTTTGTAaccac < 1:315523/140‑1 (MQ=255) tGAGCAGTTTGGTTATGTCACGCGCCGTTAAAACGTTCTGCGCCGACAATCCGGTAGGTTCAACAAAGCGCGTGTTGTTCATTCCGAGCGATTTCGCTTTCGCATTCATTGCCTTAATAAAGGCTTTGTAACCACCGGGa < 1:218554/140‑1 (MQ=255) gTTTGGTTAAGTCACGGGCAGTTGAAACGTTATGCGCCGACAATCCGGTAGGTTCAACAAAGCGCGTGTTGTTCATTCCGAGCGATTTCGCTTTCGCATTGATTACCTAAATAAAGGGTTTGTAACCACCGGGATAATgg > 2:233675/1‑140 (MQ=255) ttAAGTCACGGGCAGTTGAAACGTTATGCGCCGACAATCCGGTAGGTTCAACAAAGCGCGTGTTGTTCATTCCGAGCGATTTCGCTTTCGCATTCATTGCCTTAATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCa > 1:549953/1‑140 (MQ=255) ttAAGTCACGGGCAGTTGAAACGTTATGCGCCGACAATCCGGTAGGTTCAACAAAGCGCGTGTTGTTCATTCCGAGCGATTTCGCTTTCGCATTCATTGCCTTAATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCa > 1:645916/1‑140 (MQ=255) tCACGGGCAGTTGAAACGTTATGCGCCGACAATCCGGTAGGTTCAACAAAGCGCGTGTTGTTCATTCCGAGCGATTTCGCTTTCGCATTCATTGCCTTAATAAAGGCTTTGTAACCACCGTGATAATGGTGCGCAAGGCt > 1:839239/1‑140 (MQ=255) tCACGGGCAGTTGAAACGTTATGCGCCGACAATCCGGTAGGTTCAACAAAGCGCGTGTTGTCCATTCCGAGCGATTTCGCTTTCGCATTCAGTGCCTTAAGAAAGGCTATGTAACCAACGGGGTAATGGTGCGCAAGGCt > 2:240929/1‑140 (MQ=255) gggCAGTTGAAACGTTATGCGCCGACAATCCGGTAGGTTCAACAAAGCGCGTGTTGTTCATTCCGAGCGATTTCGCTTTCGCATTCATTGCCTTAATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTgcc > 1:555595/1‑140 (MQ=255) ttGAAACGTTATGCGCCTACAATCCGGTAGGTTCAACAACGCGCGTGTAGTTCATTCCGAGCGATTTCGCTTTCGCATTCATTGCCTTAATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCcgcg < 2:328785/140‑1 (MQ=255) aaCGTTATGCGCCGACAATCCGGTAGGTTCAACAAAGCGCGTGTTGTTCATTCCGAGCGATTTCGCTTTCGCATTCATTGCCTTAATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGt < 2:645916/140‑1 (MQ=255) aTGCGCCGACAATCCGGTAGGTTCAACAAAGCGCGTGTTGTTCATTCCGAGCGATTTCGCTTTCGCATTCATTGCCTTAATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTg > 2:473516/1‑140 (MQ=255) aTGCGCCGACAATCCGGTAGGTTCAACAAAGCGCGTGTTGTTCATTCCGAGCGATTTCGCTTTCGCATTCATTGCCTTAATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTg > 2:191682/1‑140 (MQ=255) | TGCGGAACGGCAGCGTATACGTCGGATTAGAGAAGGTTGCCATATCTTCCCGGGTAGTACTTAACTGCCCGATCAACGGATATTGTTTGCTGGCAATGAGCAGTTTGGTTAAGTCACGGGCAGTTGAAACGTTATGCACCGACAATCCGGTAGGTTCAACAAAGCGCGTGTTGTTCATTCCGAGCGATTTCGCTTTCGCATTCATTGCCTTAATAAAGGCTTTGTAACCACCGGGATAATGGTGCGCAAGGCTTGCCGCCGCGCGGTTTTCTG > NC_000913/2224228‑2224500 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |