Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,233,659 C→T F20F (TTC→TTT)  yeiS → DUF2542 domain‑containing protein YeiS

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,233,6590CT98.2% 187.9 / NA 57F20F (TTC→TTT) yeiSDUF2542 domain‑containing protein YeiS
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base T (29/27);  total (29/28)
Fisher's exact test for biased strand distribution p-value = 4.91e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACCGGAAGATGCGCAGGGGTATCCCGCCGTCACACCCGAACAGTTACTTGAATTACAAAAGAAACAAGGAAAGTAGTTATGGATGTACAGCAGTTTTTTGTCGTTGCCGTTTTTTTCCTTATCCCGATATTTTGTTTCCGCGAAGCATGGAAAGGCTGGCGCGCAGGCGCGATTGATAAACGGGTTAAAAATGCACCGGAACCGGTGTATGTCTGGCGAGCAAAAAATCCCGGACTCTTTTTCGCTTATATGGTGGCATATATCGGCTTCGGA  >  NC_000913/2233522‑2233794
                                                                                                                                         |                                                                                                                                       
aCCGGAAGATGCGCAGGGGTATTCCGCCGTCACACCCGAACAGTTACTTGAATTACAAAAGAAACAAGGAAAGTAGTTATGGATGTACAGCAGTTTTTTGTCGTTGCCGTTTTTTTCCTTATCCCGATATTTTGTTTTcg                                                                                                                                       >  2:84213/1‑140 (MQ=255)
   ggAAGATGCGCAGGGGTATCCCGCCGTCACACCCGAACAGTTACTTGAATTACAAAAGAAACAAGGAAAGTAGTTATGGATGTACAGCAGTTTTTTGTCGTTGCCGTTTTTTTCCTTATCCCGATATTTTGTTTTCGCGa                                                                                                                                    >  2:92409/1‑140 (MQ=255)
   ggAAGATGCGCAGGGGTATCCCGCCGTCACACCCGAACAGTTACTTGAATTACAAAAGAAACAAGGAAAGTAGTTATGGATGTACAGCAGTTTTTTGTCGTTGCCGTTTTTTTCCTTATCCCGATATTTTGTTTTCGCGa                                                                                                                                    <  2:109893/140‑1 (MQ=255)
         tGCGCAGGGGTATCCCGCCGTCACACCCGAACAGTTACTTGAATTACAAAAGAAACAAGGAAAGTAGTTATGGATGTACAGCAGTTTTTTGTCGTTGCCGTTTTTTTCCTTATCCCGATATTTTGTTTTcgcg                                                                                                                                     >  1:123199/1‑133 (MQ=255)
         tGCGCAGGGGTATCCCGCCGTCACACCCGAACAGTTACTTGAATTACAAAAGAAACAAGGAAAGTAGTTATGGATGTACAGCAGTTTTTTGTCGTTGCCGTTTTTTTCCTTATCCCGATATTTTGTTTTcgcg                                                                                                                                     <  2:123199/133‑1 (MQ=255)
                     tCCCGCCGTCACACCCGAACAGTTACTTGAATTACAAAAGAAACAAGGAAAGTAGTTATGGATGTACAGCAGTTTTTTGTCGTTGCCGTTTTTTTCCTTATCCCGATATTTTGTTTTCGCGAAg                                                                                                                                  <  1:6362/124‑1 (MQ=255)
                     tCCCGCCGTCACACCCGAACAGTTACTTGAATTACAAAAGAAACAAGGAAAGTAGTTATGGATGTACAGCAGTTTTTTGTCGTTGCCGTTTTTTTCCTTATCCCGATATTTTGTTTTCGCGAAg                                                                                                                                  >  2:6362/1‑124 (MQ=255)
                     tCCCGCCGTCACACCCGAACAGTTACTTGAATTACAAAAGAAACAAGGAAAGTAGTTATGGATGTACAGCAGTTTTTTGTCGTTGCCGTTTTTTTCCTTATCCCGATATTTTGTTTTCGCGAAGCATGGAAAGGATGgcg                                                                                                                  >  1:52316/1‑140 (MQ=255)
                          ccgTCACACCCGAACAGTTACTTGAATTACAAAAGAAACAAGGAAAGTAGTTATGGATGTACAGCAGTTTTTTGTCGTTGCCGTTTTTTTCCTTATCCCGATATTTTGTTTTCGCGAAGCATGGAAAGGCTGGCGCGCAg                                                                                                             >  2:688016/1‑140 (MQ=255)
                           cgTCACACCCGAACAGTTACTTGAATTACAAAAGAAACAAGGAAAGTAGTTATGGATGTACAGCAGTTTTTTGTCGTTGCCGTTTTTTTCCTTATCCCGATATTTTGTTTTCGCGAAGCATGGAAAGGCTGGCGCGCAgg                                                                                                            >  2:663068/1‑140 (MQ=255)
                             tCACACCCGAACAGTTACTTGAATTACAAAAGAAACAAGGAAAGTAGTTATGGATGTACAGCAGTTTTTTGTCGTTGCCGTTTTTTTCCTTATCCCGATATTTTGTTTTCGCGAAGCATGGAAAGGCTGGCGCGCAGgcg                                                                                                          <  1:92409/140‑1 (MQ=255)
                             tCACACCCGAACAGTTACTTGAATTACAAAAGAAACAAGGAAAGTAGTTATGGATGTACAGCAGTTTTTTGTCGTTGCCGTTTTTTTCCTTATCCCGATATTTTGTTTTCGCGAAGCATGGAAAGGCTGGCGCGCAGgcg                                                                                                          >  1:136821/1‑140 (MQ=255)
                                      aaCAGTTACTTGAATTACAAAAGAAACAAGGAAAGTAGTTATGGATGTACAGCAGTTTTTTGTCGTTGCCGTTTTTTTCCTTATCCCGATATTTTGTTTTCGCGAAGCATGGAAAGGCTGGCGCGCAGGCGCGATTGATa                                                                                                 <  1:105452/140‑1 (MQ=255)
                                        cAGTTACTTGAATTACAAAAGAAACAAGGAAAGTAGTTATGGATGTACAGCAGTTTTTTGTCGTTGCCGTTTTTTTCCTTATCCCGATATTTTGTTTTCGCGAAGCATGGAAAGGCTGGCGCGCAGGCGCGATTGATaaa                                                                                               <  2:624959/140‑1 (MQ=255)
                                        cAGTTACTTGAATTACAAAAGAAACAAGGAAAGTAGTTATGGATGTACAGCAGTTTTTTGTCGTTGCCGTTTTTTTCCTTATCCCGATATTTTGTTTTCGCGAAGCATGGAAAGGCTGGCGCGCAGGCGCGATTGATaaa                                                                                               <  2:655249/140‑1 (MQ=255)
                                                   aTTACAAAAGAAACAAGGAAAGTAGTTATGGATGTACAGCAGTTTTTTGTCGTTGCCGTTTTTTTCCTTATCCCGATATTTTGTTTTCGCGAAGCATGGATAGGCTGGTCCGCAGGCTCGATTAATAAACGAGTTaaaaa                                                                                    >  2:401719/1‑140 (MQ=255)
                                                        aaaaGAAACAAGGAACGTAGTTATGCATGTACAGCAGATTTTTGTCGTTGCCGTTTTTTTCCTTATCCCGATATTTTGTTTTCGCGAAGCATGGAAAGGCTGGCGCGCAGGCGCGATTGATAAACGGGTTAAAAATGCAc                                                                               <  1:401719/140‑1 (MQ=255)
                                                             aaaCAAGGAAAGTAGTTATGGATGTACAGCAGTTTTTTGTCGTTGCCGTTTTTTTCCTTATCCCGATATTTTGTTTTCGCGAAGCATGGAAAGGCTGGCGCGCAGGCGCGATTGATAAACGGGTTAAAAATGCACCGGaa                                                                          <  2:647971/140‑1 (MQ=255)
                                                              aaCAAGGAAGGTAGTTATGGATGTACAGCAGTTTTTTGTCGTTGCCGTTTTTTTCCTTATCCCGATATTTTGTTTTCGCGAAGCATGGAAAGGCTGGCGCGCAGGCGCGATTGATAAACGGGTTAAAAATGCACCGGAAc                                                                         <  1:817713/140‑1 (MQ=255)
                                                              aaCAAGGAAAGTAGTTATGGATGTACAGCAGTTTTTTGTCGTTGCCGTTTTTTTCCTTATCCCGATATTTTGTTTTCGCGAAGCATGGAAAGGCTGGCGCGCAGGCGCGATTGATAAACGGGTTAAAAATGCACCGGAAc                                                                         <  2:149712/140‑1 (MQ=255)
                                                              aaCAAGGAAAGTAGTTATGGATGTACAGCAGTTTTTTGTCGTTGCCGTTTTTTTCCTTATCCCGATATTTTGTTTTCGCGAAGCATGGAAAGGCTGGCGCGCAGGCGCGATTGATAAACGGGTTAAAAATGCACCGGAAc                                                                         <  2:753785/140‑1 (MQ=255)
                                                              aaCAAGGAAAGTAGTTATGGATGTACAGCAGTTTTTTGTCGTTGCCGTTTTTTTCCTTATCCCGATATTTTGTTTTCGCGAAGCATGGAAAGGCTGGCGCGCAGGCGCGATTGATAAACGGGTTAAAAATGCACCGGAAc                                                                         <  1:84213/140‑1 (MQ=255)
                                                              aaCAAGGAAAGTAGTTAGGGATTTACAGCAGGTTTTTGTCGTTGCCGTTTTTTTCCTTATCCCGATATTTTGTTTTCGCGAAGCATGGAAAGGCTGGCGCGCAGGCGCGATTGATAAACGGGTTAAAAATGCACCGGAAc                                                                         <  2:292954/140‑1 (MQ=255)
                                                                  aGGAAAGTAGTTATGGATGTACAGCAGTTTTTTGTCGTTGCCGTTTTTTTCCTTATCCCGATATTTTGTTTTCGCGAAGCATGGAAAGGCTGGCGCGCAGGCGCGATTGATAAACGGGTTAAAAATGCACCGGAACCGgt                                                                     <  1:467979/140‑1 (MQ=255)
                                                                   ggAAAGTAGTTATGGATGTACAGCAGTTTTTTGTCGTTGCCGTTTTTTTCCTTATCCCGATATTTTGTTTTCGCGAAGCATGGAAAGGCTGGCGCGCAGGCGCGATTGATAAACGGGTTAAAAATGCACCGGAACCGgtg                                                                    <  2:719517/140‑1 (MQ=255)
                                                                           gtTATGGATGTACAGCAGTTTTTTGTCGTTGCCGTTTTTTTCCTTATCCCGATATTTTGTTTTCGCGAAGCATGGAAAGGCTGGCGCGCAGGCGCGATTGATAAACGGGTTAAAAATGCACCGGAAc                                                                         <  1:745243/127‑1 (MQ=255)
                                                                           gtTATGGATGTACAGCAGTTTTTTGTCGTTGCCGTTTTTTTCCTTATCCCGATATTTTGTTTTCGCGAAGCATGGAAAGGCTGGCGCGCAGGCGCGATTGATAAACGGGTTAAAAATGCACCGGAAc                                                                         >  2:745243/1‑127 (MQ=255)
                                                                              aTGGATGTACAGCAGTTTTTTGTCGTTGCCGTTTTTTTCCTTATCCCGATATTTTGTTTTCGCGAAGCATGGAAAGGCTGGCGCGCAGGCGCGATTGATACACGGGTTGAAAATGCAGCGGAACCGGTGTATGTCTGGCg                                                         >  2:371524/1‑140 (MQ=255)
                                                                              aTGGATGTACAGCAGTTTTTTGTCGTTGCCGTTTTTTTCCTTATCCCGATATTTTGTTTTCGCGAAGCATGGAAAGGCTGGCGCGCAGGCGCGATTGATAAACGGGTTAAAAATGCACCGGAACCGGTGTATGTCTGGCg                                                         >  2:621860/1‑140 (MQ=255)
                                                                              aTGGATGTACAGCAGTTTTTTGTCGTTGCCGTTTTTTTCCTTATCCCGATATTTTGTTTTCGCGAAGCATGGAAAGGCTGGCGCGCAGGCGCGATTGATAAACGGGTTAAAAATGCACCGGAACCGGTGTATGTCTGGCg                                                         >  2:817133/1‑140 (MQ=255)
                                                                              aTGGATGTACAGCAGTTTTTTGTCGTTGCCGTTTTTTTCCTTATCCCGATATTTTGTTTTCGCGAAGCATGGAAAGGCTGGCGCGCAGGCGCGATTGATAAACGGGTTAAAAATGCAACGGAACCGGTGTATGTCTGGCg                                                         >  1:349116/1‑140 (MQ=255)
                                                                               tGGATGTACAGCAGTTTTTTGTCGTTGCCGTTTTTTTCCTTATCCCGATATTTTGTTTTCGCGAAGCATGGAAAGGCTGGCGCGCAGGCGCGATTGATAAACGGGTTAAAAATGTACCGGAACCAGTGTATGTCTGGCGa                                                        >  1:289039/1‑140 (MQ=255)
                                                                                ggATGTACAGCAGTTTTCTGTCCTTGCCGTTGTTTGCCTTATCCCGATGTTTTGTTTTCGCGAAGCATGGAAAGGCTGGCGCGCAGGCGCGATTGATAAACGGGTTAAAAATGCACCGGAACCGGTGTATGTCTGGCGAg                                                       <  2:349116/140‑1 (MQ=255)
                                                                                   tGTACAGCAGTTTTTTGTCGTTGCCGTTTTTTTCCTTATCCCGATATTTTGTTTTCGCGAAGCATGGAAAGGCTGGCGCGCAGGCGCGATTGATAAACGGGTTACAAACGCACCGGATCCGGCGTATGTCTGGCGAGCaa                                                    >  2:334786/1‑140 (MQ=255)
                                                                                   tGTACAGCAGTTTTTTGTCGTTGCCGTTTTTTTCCTTATCCCGATATTTTGTTTTCGCGAAGCATGGAAAGGCTGGCGCGCAGGCGCGATTGATAAACGGGTTAAAAATGCACCGGAACCGGTGTATGTCTGGCg                                                         <  1:484108/135‑1 (MQ=255)
                                                                                   tGTACAGCAGTTTTTTGTCGTTGCCGTTTTTTTCCTTATCCCGATATTTTGTTTTCGCGAAGCATGGAAAGGCTGGCGCGCAGGCGCGATTGATAAACGGGTTAAAAATGCACCGGAACCGGTGTATGTCTGGCg                                                         >  2:484108/1‑135 (MQ=255)
                                                                                   tGTACAGCAGTTTTTTGTCGTTGCCGTTTTTTTCCTTATCCCGATATTTTGTTTTCGCGAAGCATGGAAAGGCTGGCGCGCAGGCGCGATTGATAAACGGGTTAAAAATGCACCGGAACCGGTGTATGTCTGGCGAGCaa                                                    >  1:504882/1‑140 (MQ=255)
                                                                                    gTACAGCAGTTTTTTGTCGTTGCCGTTTTTTTCCTTATCCCGATATTTTGTTTTCGCGAAGCATGGAAAGGCTGGCGCGCAGGCGCGATTGATAAACGGGTTAAAAATGCCCCGGAATCGGTGTATGTCTGGCGAGCaaa                                                   >  1:421890/1‑140 (MQ=255)
                                                                                     tACAGCAGTTTTTTGTCGTTGCCGTTTTTTTCCTTATCCCGATATTTTGTTTTCGCGAAGCATGGAAAGGCTGGCGCGCAGGCGCGATTGATAAACGGGTTAAAAATGCACCGGAACCGGTGTATGTCTGGCGAGCaaaa                                                  >  1:771467/1‑140 (MQ=255)
                                                                                         gcagTTTTTTGTCGTTGCCGTTTTTTTCCTTATCCCGATATTTTGTTTTCGCGAAGCATGGAAAGGCTGGCGCGCAGGCGCGATTGATAAACGGGTTAAAAATGCACCGGAACCGGTGTATGTCTGGCGAGCAAAAAATc                                              >  1:447106/1‑140 (MQ=255)
                                                                                            gTTTTTTGTCGTTGCCGTTTTTTTCCTTATCCCGATATTTTGTTTTCGCGAAGCATGGAAAGGCTGGCGCGCAGGCGCGATTGATAAACGGGTTAAAAATGCACCGGAACCGGTGTATGTCTGGCGAGCAAAAAATCCCg                                           >  1:12702/1‑140 (MQ=255)
                                                                                              tttttGTCGTTGCCGTTTTTTTCCTTATCCCGATATTTTGTTTTCGCGAAGCATGGAAAGGATGGCGCGCAGGCGCGATTGATAAACGGGTTAAAAATGCACCGGAACCGGTGTATGTCTGGCGAGCAAAAAATCCCGGa                                         <  2:52316/140‑1 (MQ=255)
                                                                                               ttttGTCGTTGCCGTTTTTTTCCTTATCCCGATATTTTGTTTTCGCGAAGCATGGAAAGGCTGGCGCGCAGGCGCGATTGATAAACGGGTTTAAAATACAACGGGACCTGTGTATGTCTGGCACGCAAAAAATCCCGGAc                                        >  2:416844/1‑140 (MQ=255)
                                                                                               ttttGTCGTTGCCGTTTTTTTCCTTATCCCGATATTTTGTTTTCGCGAAGCATGGAAAGGCTGGCGCGCAGGCGCGATTGATAAACGGGTTAAAAATGCACCGGAACCGGTGTATGTCTGGCGAGCAAAAAATCCCGGAc                                        >  2:543033/1‑140 (MQ=255)
                                                                                               ttttGTCGTTGCCGTTTTTTTCCTTATCCCGATATTTTGTTTTCGCGAAGCATGGAAAGGCTGGCGCGCAGGCGCGATTGATAAACGGGTTAAAAATGCACCGGAACCGGTGTATGTCTGGCGAGCAAAAAATCCCGGAc                                        >  2:15103/1‑140 (MQ=255)
                                                                                               ttttGTCGTTGCCGTTTTTTTCCTTATCCCGATATTTTGTTTTCGCGAAGCATGGAAAGGCTGGCGCGCAGGCGCGATTGATAAACGGGTTAAAAATGCACCGGAACCGGTGTATGTCTGGCGAGCAAAAAATCCCGGAc                                        >  2:168296/1‑140 (MQ=255)
                                                                                               ttttGTCGTTGCCGTTTTTTTCCTTATCCCGATATTTTGTTTTCGCGAAGCATGGAAAGGCTGGCGCGCAGGCGCGATTGATAAACGGGTTAAAAATGCACCAGAACCGGAGTACGTCTGGCGATCAAAAAATCCCGGAc                                        >  1:361614/1‑140 (MQ=255)
                                                                                                      gTTGCCGTTTTTTTCCTTATCCGGATATTTTTTTTTCGCTAAGCATGGAAAGGCTGGCGCGCAGGCGCGATTGATAAACGGGTTAAAAATGCACCGGAACCGGTGTATGTCTGGCGAGCAAAAAATCCCGGACTCttttt                                 <  2:289039/140‑1 (MQ=255)
                                                                                                      gTTGCCGTTTTTTTCCTTATCCCGATATTTTGTTTTCGCGAAGCATGGAAAGGCTGGCGCGCAGGCGCGATTGATAAACGGGTTAAAAATGCACCGGAACCGGTGTATGTCTGGCGAGCAAAAAATCCCGGACTCttttt                                 <  1:543033/140‑1 (MQ=255)
                                                                                                       ttGCCGTTTTTTTCCTTATCCCGATATTTTGTTTTCGCGAAGCATGGAAAGGCTGGCGCGCAGGCGCGATTGATAAACGGGTTAAAAATGCACCGGAACCGGTGTATGTCTGGCGAGCAAAAAATCCCGGACTCTTTTTc                                >  2:864262/1‑140 (MQ=255)
                                                                                                         gCCGTTTTTTTCCTTATCCCGATATTTTGTTTTCGCGAAGCATGGAAAGGCTGGCGCGCAGGCGCGATTGATAAACGGGTTAAAAATGCACCGGAACCGGTGTATGTCTGGCGAGCAAAAAATCCCGGACTCTTTTTCGc                              >  2:571653/1‑140 (MQ=255)
                                                                                                         gCCGTTTTTTTCCTTATCCCGATATTTTGTTTTCGCGAAGCATGGAAAGGCTGGCGCGCAGGCGCGATTGATAAACGGGTTAAAAATGCACCGGAACCGGTGTATGTCTGGCGAGCAAAAAATCCCGGACTCTTTTTCGc                              <  2:771467/140‑1 (MQ=255)
                                                                                                         gCCGTTTTTTTCCTTATCCCGATATTTTGTTTTCGCGAAGCATGGAAAGGCTGGCGCGCAGGCGCGATTGATAAACGGGTTAAAAATGCACCGGAACCGGTGTATGTCTGGCGAGCAAAAAATCCCGGACTCTTTTTCGc                              <  2:504882/140‑1 (MQ=255)
                                                                                                            gTTTTTTTCCTTATCGCGATATTTTATTTACGCGAAGTATGGAAAGGCTGGCGCGCAGGCGCGATTGATAAACGGGTTAAAAATGCACCGGAACCGGTGTATGTCTGGCGAGCAAAAAATCCCGGACTCTTTTTCGCTta                           <  1:371524/140‑1 (MQ=255)
                                                                                                            gTTTTTTTCCTTATCCCGATATTTTGTTTCCGCGAAGCATGGAAAGGCTGGCGCGCAGGCGCGATTGATAAACGGGTTAAAAATGCACCGGAACCGGTGTATGTCTGGCGAGCAAAAAATCCCGGACTCTTTTTCGCTta                           <  1:334786/140‑1 (MQ=255)
                                                                                                                ttttCCTTAACCCGAAATTTTGTTTTCGCAAAGCATGGAAAGGCTGGCGCGCAGGCGCGATTGATAAACGGGTTAAAAATACACCGGAACCGGTGTATGTCTGGCGAGCAAAAAATCCCGGACTCTTTTTCGCTTATAtg                       <  1:416844/140‑1 (MQ=255)
                                                                                                                                  tttGTTTTCGCGAAGCTGGGAACGGCTGGCACGAACGCGGGATTGATATACGGGTTAAAAATGCACCGGAACCGGTGTATGTCTGGCGAGCAAAAAATCCCGGACTCTTTTTCGCTTATATGGTGGCATATATCGGCTTc     <  2:421890/140‑1 (MQ=255)
                                                                                                                                   ttGTTTTCGCGAAGCATGGAAAAGCCGGCCCGCAGGCGCGATTGATAAACGGGTTAAAAATGCACCGGAACCGGTGTATGTCTGGCGAGCAAAAAATCCCGGACTCTTTTTCGCTTATATGGTGGCATATATCGGCTTCg    <  1:301754/140‑1 (MQ=255)
                                                                                                                                     gTTTTCGCGAAGCATGGAAAGGCTGGCGCGCAGGCGCGATTGATAAACGGGTTAAAAATGCACCGGAACCGGTGTATGTCTGGCGAGCAAAAAATCCCGGACTCTTTTTCGCTTATATGGTGGCATATATCGGCTTCGGa  <  1:168296/140‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                       
ACCGGAAGATGCGCAGGGGTATCCCGCCGTCACACCCGAACAGTTACTTGAATTACAAAAGAAACAAGGAAAGTAGTTATGGATGTACAGCAGTTTTTTGTCGTTGCCGTTTTTTTCCTTATCCCGATATTTTGTTTCCGCGAAGCATGGAAAGGCTGGCGCGCAGGCGCGATTGATAAACGGGTTAAAAATGCACCGGAACCGGTGTATGTCTGGCGAGCAAAAAATCCCGGACTCTTTTTCGCTTATATGGTGGCATATATCGGCTTCGGA  >  NC_000913/2233522‑2233794

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑