Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,234,448 C→T A139V (GCC→GTC)  preT → NAD‑dependent dihydropyrimidine dehydrogenase subunit PreT

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,234,4480CT97.8% 142.4 / NA 45A139V (GCC→GTC) preTNAD‑dependent dihydropyrimidine dehydrogenase subunit PreT
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (25/19);  minor base A (1/0);  total (26/19)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTCCCATTGATATCGGCCGCTTACAGCGTTTTGTTACTGATTTTGAACAACAAACCGGAATGGAAATTTATCAGCCCGGTACTAAAACGCTCGGCAAAGTCGCGATTATTGGCGCAGGTCCTGCCGGATTGCAGGCCAGTGTGACACTGACAAACCAGGGTTATGACGTCACGATTTATGAGAAAGAAGCGCACCCCGGTGGCTGGTTGCGTAACGGTATTCCGCAATTCCGGTTACCGCAATCAGTGCTGGATGCAGAGATCGCCCGTATT  >  NC_000913/2234313‑2234584
                                                                                                                                       |                                                                                                                                        
cTCCCATTGATATCGGCCGCTTACAGCGTTTTGTTACTGATTTTGAACAACAAACCGGAATGGAAATTTATCAGCCCGGTACTAAAACGCTCGGCAAAGTCGCGATTATTGGCGCAGGTCCTGCCGGATTGCAGGTCAgt                                                                                                                                      >  1:591215/1‑140 (MQ=255)
cTCCCATTGATATCGCCCGCTTCCACCGTTTTGTTACAGATTTTGAACAACAAACCGGAATGGAAATTTATCAGCCCGGTACTAAAACGCTCGGCAAAGTCGCGATTATTGGCGCAGGTCCTGCCGGATTGCAGGTCAgt                                                                                                                                      <  1:298472/140‑1 (MQ=255)
 tCCCATTGATATCGGCCGCTTACAGCGTTTTGTTACTGATTTTGAACAACAAACCGGAATGGAAATTTATCAGCCCGGTACTAAAACGCTCGGCAAAGTCGCGATTATTGGCGCAGGTCCTGCCGGATTGCAGGTCAgtg                                                                                                                                     <  2:545038/140‑1 (MQ=255)
  cccATTGATATCGGCCGCTTACAGCGTTTTGTTACTGATTTTGAACAACAAACCGGAATGGAAATTTATCAGCCCGGTACTAAAACGCTCGGCAAAGTCGCGGTTATTGGAGCAGGTACTGCCGTATTGCAGGTCAgtgt                                                                                                                                    >  1:225722/1‑140 (MQ=255)
    cATTGATATCGGCCGCTTACAGCGTTTTGTTACTGATTTTGAACAACAAACCGGAATGGAAATTTATCAGCCCGGTACTAAAACGCTCGGCCAAGTCGCGGTTAATGGAGCAGGTCCTGCCGGATTGCAGGTCAGTGTGa                                                                                                                                  >  2:298472/1‑140 (MQ=255)
    cATTGATATCGGCCGCTTACAGCGTTTTGTTACTGATTTTGAACAACAAACCGGAATGGAAATTTATCAGCCCGGTACTAAAACGCTCGGCAAAGTCGCGATTATTGGCGCAGGTCCTGCCGGATTGCAGGTCAGTGTGa                                                                                                                                  >  1:655507/1‑140 (MQ=255)
     aTTGATATCGGCCGCTTACAGCGTTTTGTTACTGATTTTGAACAACAAACCGGAATGGAAATTTATCAGCCCGGTACTAAAACGCTCGGCAAAGTCGCGATTCTTGGCGCGGGTGCTACCGGATCGCAGGTCAGTGTGac                                                                                                                                 >  1:229807/1‑140 (MQ=255)
          tatCGGCCGCTTACAGCGTCTTGTTACTGATTTTGAACAACAAACCGGAATGGAAATTTATCAGCCCGGTACTAAAACGCTCGGCAAAGTCGCGATTATTGGCGCAGGTCCTGCCGGATTGCAGGTCAGTGTGACACTGa                                                                                                                            <  1:490522/140‑1 (MQ=255)
          tatCGGCCGCTTACAGCCTTTTCTTACTGATTTTGCACATCAAACCGGCATGGAAATTTATCAGCCCGGTACTAAAACGCTCGGCAAAGTCGCGATTATTGGCGCAGGTCCTGCCGGATTGCAGGTCAGTGTGACACTGa                                                                                                                            <  2:225722/140‑1 (MQ=255)
           atCGGCCGCTTACAGCGTTTTGTTACTGATTTTGAACAACAAACCGGAATGGAAATTTATCAGCCCGGTACTAAAACGCTCGGCAAAGTCGCGATTATTGGCACAGGTCCGGCCGGAGTGCAGGACAGTGTGACACTGAc                                                                                                                           >  1:229598/1‑140 (MQ=255)
             cGGCCGCTTACAGCGTTTTGTTACTGATTTTGAACAACAAACCGGAATGGAAATTTATCAGCCCGGTACTAAAACGCTCGGCAAAGTCGCGATTATTGGCGCAGGTCCTGCCGGATTGCAGGTCAGTGTGACACTGACaa                                                                                                                         >  2:587076/1‑140 (MQ=255)
                   cTTACAGCGTTTTGTTACTGATTTTGAACAACAAACCGGAATGGAAATTTATCAGCCCGGTACTAAAACGCTCGGCAAAGTCGCGATTATTGGCGCAGGTCCTGCCGGATTGCAGGTCAGTGTGACACTGACAAACCAgg                                                                                                                   >  2:639245/1‑140 (MQ=255)
                    ttACAGCGTTTTGTTACTGATTTTGAACAACAAACCGGAATGGAAATTTATCAGCCCGGTACTAAAACGCTCGGCAAAGTCGCGATTATTGGCGCAGGTCCTGCCGGATTCCAGTTCTGTGTGACACTGACAAACCAggg                                                                                                                  >  1:309312/1‑140 (MQ=255)
                      aCAGCGTTTTGTTACTGATTTTGAACAACAAACCGGAATGGAAATTTATCAGCCCGGTACTAAAACGCTCGGCAAAGTCGCGATTATTGGCGCAGGTCCTGCCGGATTGCAGGTCAGTGTGACACTGACAAACCAGGGtt                                                                                                                <  1:639245/140‑1 (MQ=255)
                              ttGTTACTGATTTTGAACAACAAACCGGAATGGAAATTTATCAGCCCGGTACTAAAACGCTCGGCAAAGTCGCGATTATTGGCGCAGGTCCTGCCGGATTGCAGGTCAGTGTGACACTGACAAACCAGGGTTATGACGTc                                                                                                        >  1:812069/1‑140 (MQ=255)
                               tGTTACTGATTTTGAACAACAACCCGGAATGGAAATTTAGCAGCCCGGCACTAAAACGCTCGGCAAAGTCGCGATTATTGGCGCAGGTCCTGCCGGATTGCAGGTCAGTGTGACACTGACAAACCAGGGTTATGACGTCa                                                                                                       <  2:354738/140‑1 (MQ=255)
                               tGTTACTGATTTTGAACAACAAACCGGAATGGAAATTTATCAGCCCGGTACTAAAACGCTCGGCAAAGTCGCGATTATTGGCGCAGGTCCTGCCGGATTGCAGGTCAGTGTGACACTGACAAACCAGGGTTATGACGTCa                                                                                                       <  2:591215/140‑1 (MQ=255)
                               tGTTACTGATTTTGAACAACAAACCGGAATGGAAATTTATCAGCCCGGTACTAAAACGCTCGGCAAAGTCGCGATTATTGGCGCAGGTCCTGCCGGATTGCAGGTCAGTGTGACACTGACAAACCAGGGTTATGACGTCa                                                                                                       >  1:636790/1‑140 (MQ=255)
                                gTTACTGATTTTGAACAACAAACCGGAATGGAAATTTATCAGCCCGGTACTAAAACGCTCGGCAAAGTCGCGATTATTGGCGCAGGTCCTGCCGGATTGCAGGTCAGTGTGACACTGACAAACCAGGGTTATGACGTCAc                                                                                                      <  1:587076/140‑1 (MQ=255)
                                 ttACTGATTTTGAACAACAAACCGGAATGGAAATTTATCAGCCCGGTACTAAAACGCTCGGCAAAGTCGCGATTATTGGCGCAGGTCCTGCCGGATTGCAGGTCAGTGTGACACTGACAAACCAGGGTTATGACGTCACg                                                                                                     <  1:138432/140‑1 (MQ=255)
                                      gATTTTGAACAACAAACCGGAATGGAAATTTATCAGCCCGGTACTAAAACGCTCGGCAAAGTCGCGATTATTGGCGCAGGTCCTGCCGGATTGCAGGTCAGTGTGACACTGACAAACTAGGGTTATGACGTCACGATTTa                                                                                                >  1:354738/1‑140 (MQ=255)
                                      gATTTTGAACAACAAACCGGAATGGAAATTTATCAGCCCGGTACTAAAACGCTCGGCAAAGTCGCGATTATTGGCGCAGGTCCTGCCGGATTGCAGGTCAGTGTGACACTGACAAACCAGGGTTATGACGTCa                                                                                                       <  2:624474/133‑1 (MQ=255)
                                      gATTTTGAACAACAAACCGGAATGGAAATTTATCAGCCCGGTACTAAAACGCTCGGCAAAGTCGCGATTATTGGCGCAGGTCCTGCCGGATTGCAGGTCAGTGTGACACTGACAAACCAGGGTTATGACGTCa                                                                                                       >  1:624474/1‑133 (MQ=255)
                                       aTTTTGAACAACAAACCGGAATGGAAATTTATCAGCCCGGTACTAAAACGCTCGGCAAAGTCTCGTTTATTGTCGCAGGTCCTGCCCGATGGCAGGTCAGTGTGACACTGACAAACCAGGGTTATGACGTCACGATTTaa                                                                                               >  2:68502/1‑139 (MQ=255)
                                        ttttGAACAACAAACCGGAATGGAAATTTATCAGCCCGGTACTAAAACGCTCGGCAAAGTCGCGATTATTGGCGCAGGTCCTGCCGGATTGCAGGTCAGTGTGACACTGACAAACCAGGGTTATGACGTCACGATTTATg                                                                                              >  1:17695/1‑140 (MQ=255)
                                           tGAACAACAAACCGGAATGGAAATTTATCAGCCCGGTACTAAAACGCTCGGCAAAGTCGCGATTATTGGCGCAGGTCCTGCCGGATTGCAGGTCAGTGTGACACTGACAAACCAGGGTTATGACGTCACGATTTATgaga                                                                                           >  1:694280/1‑140 (MQ=255)
                                                             ggAAATTTATCAGCCCGGTACTAAAACGCTCGGCAAAGTCGCGATTATTGGCGCAGGTCCTGCCGGATTGCAGGTCAGTGTGACACTGACAAACCAGGGTTATGACGTCACGATTTATGAGAAAGAAGCGCACCCCGGTg                                                                         >  2:594214/1‑140 (MQ=255)
                                                                  tttATCAGCCCGGTACTAAAACGCTCGGCAAAGTCGCGATTATTGGCGCAGGTCCTGCCGGATTGCAGGTCAGTGTGACACTGACAAACCAGGGTTATGACGTCACGATTTATGAGAAAGAAGCGCACCCCGGTGGCTgg                                                                    <  1:117332/140‑1 (MQ=255)
                                                                  tttATCAGCCCGGTACTAAAACGCTCGGCAAAGTCGCGATTATTGGCGCAGGTCCTGCCGGATTGCAGGTCAGTGTGACACTGACAAACCAGGGTTATGACGTCACGATTTATGAGAAAGAAGCGCACCCCGGTGGCTgg                                                                    <  2:655507/140‑1 (MQ=255)
                                                                         gCCCGGTACTAAAACGCTCGGCAAAGTCGCGATTATTGGCGCAGGTCCTGCCGGATTGCAGGTCAGTGTGACACTGACAAACCAGGGTTATGACGTCACGATATATGAGAAAGAAGCGCACCCCAGTGGCTGGTTGCGTa                                                             >  1:415365/1‑140 (MQ=255)
                                                                                    aaaCGCTCGGCAAAGTCGCGATTATTGGCGCCGGTACTGCCGGATTGCTGGTCAGTGTGACACTGACAAACCAGGGTTATGACGTCACGATTTATGAGAAAGAAGCGCACCCCGGTGGCTGGTTGCGTAACGGTATTCCg                                                  <  2:309312/140‑1 (MQ=255)
                                                                                    aaaCGCTCGGCAAAGTCGCGATTATTGGCGCAGGTCCTGCCGGATTGCAGGTCAGTGTGACACTGACAAACCAGGGTTATGACGTCACGATTTATGAGAAAGAAGCGCACCCCGGTGGCTGGTTGCGTAACGGTATTCCg                                                  >  2:834684/1‑140 (MQ=255)
                                                                                    aaaCGCTCGGCAAAGTCGCGATTATTGGCGCAGGTCCTGCCGGATTGCAGGTCAGTGTGACACTGACAAACCAGGGTTATGACGTCACGATTTATGAGAAAGAAGCGCACCCCGGTGGCTGGTTGCGTAACGGTATTCCg                                                  >  1:440651/1‑140 (MQ=255)
                                                                                      aCGCTCGGCAAAGTCGCGATTATTGGCGCAGGTCCTGCCGGATTGCAGGTCAGTGTGACACTGACAAACCAGGGTTATGACGTCACGATTTATGAGAAAGAAGCGCACCCCGGTGGCTGGTTGCGTAACGGTATTCCGCa                                                <  2:17695/140‑1 (MQ=255)
                                                                                         cTCGGCAAAGTCGCGATTATTGGCGCAGGTCCTGCCGGATTGCAGGTCAGTGTGACACTGACAAACCAGGGTTATGACGTCACGATTTATGAGAAAGAAGTGCAACGCGGTGGCTGGCTGCGAAACGGTATTCCGCAAtt                                             >  2:391011/1‑140 (MQ=255)
                                                                                                      cgATTATTGGCGCAGGTCCTGCCGGATTGCAGGTCAGTGTGACACTGACAAACCAGGGTTATGACGTCACGATTTATGAGAAAGAAGCGCACCCCGGTGGCTGGTTGCGTAACGGTATTCCGCAATTCCGGTTACCGCaa                                >  1:58356/1‑140 (MQ=255)
                                                                                                             tGGCGCAGGTCCTGCCGGATTGCAGGTCAGTGTGACACTGACAAACCAGGGTTATGACGTCACGATTTATGAGAAAGAAGCGCACCCCGGTGGCTGGTTGCGTAACGGTATTCCGCAATTCCGGTTACCGCAATCAGTGc                         <  1:594214/140‑1 (MQ=255)
                                                                                                              ggCGCAGGTCCTGCCCGATTGCAGGTCAGAGTGACACTGACAAACCAGGGTTATGACGTCACGATTTATGAGAAAGAAGCGCACCCCGGTGGCTGGTTGCGTAACGGTATTCCGCAATTCCGGTTACCGCAATCAGTGCt                        <  1:391011/140‑1 (MQ=255)
                                                                                                                   aGGTCCTGCCGGATTGCAGGTCAGTGTGACACTGACAAACTAGGGTTATGACGTCACGATTTATGAGAAAGAAGCGCACCCCGGTGGCTGGTTGCGTAACGGTATTCCGCAATTCCGGTTACCGCAATCAGTGCTGGATg                   >  2:545807/1‑140 (MQ=255)
                                                                                                                   aGGTCCTGCCGGATTGCAGGTCAGTGTGACACTGACAAACCAGGGTTATGACGTCACGATTTATGAGAAAGAAGCGCACCCCGGTGGCTGGTTGCGTAACGGTATTCCGCAATTCCGGTTACCGCAATCAGTGCTGGATg                   >  2:526638/1‑140 (MQ=255)
                                                                                                                      tCCTGCCGGATTGCAGGTCAGTGTGACACTGACAAACCAGGGTTATGACGTCACGATTTATGAGAAAGAAGCGCACCCCGGTGGCTGGTTGCGTAACGGTATTCCGCAATTCCGGTTACCGCAATCAGTGCTGGATGCag                <  2:812069/140‑1 (MQ=255)
                                                                                                                          gCCGGATTGCAGGTCAGTGTGACACTGACAAACCAGGGTTATGACGTCACGATTTATGAGAAAGAAGCGCACCCCGGTGGCTGGTTGCGTAACGGTATTCCGCAATTCCGGTTACCGCAATCAGTGc                         >  2:602211/1‑127 (MQ=255)
                                                                                                                          gCCGGATTGCAGGTCAGTGTGACACTGACAAACCAGGGTTATGACGTCACGATTTATGAGAAAGAAGCGCACCCCGGTGGCTGGTTGCGTAACGGTATTCCGCAATTCCGGTTACCGCAATCAGTGc                         <  1:602211/127‑1 (MQ=255)
                                                                                                                               aTTGCAGGTCAGTGTGACACTGACAAACCAGGGTTATGACGTCACGATTTATGAGAAAGAAGCGCACCCCGGTGGCTGGTTGCGTAACGGTATTCCGCAATTCCGGTTACCGCAATCAGTGCTGGATGCAGAGATCGccc       >  1:24029/1‑140 (MQ=255)
                                                                                                                               aTTGCAGGTCAGTGTGACACTGACAAACCAGGGTTATGACGTCACGATTTATGAGAAAGAAGCGCACCCCGGTGGCTGGTTGCGTAACGGTATTCCGCAATTCCGGTTACCGCAATCAGTGCTGGATGCAGAGATCGccc       >  2:821702/1‑140 (MQ=255)
                                                                                                                                    aGGTCAGTGTGACACTGACAAACCAGGGTTATGACGTCACGATTTATGAGAAAGAAGCGCACCCCGGTGGCTGGTTGCGTAACGGTATTCCGCAATTCCGGTTACCGCAATCAGTGCTGGATGCAGAGATCGCCCGTAtt  <  2:694280/140‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                        
CTCCCATTGATATCGGCCGCTTACAGCGTTTTGTTACTGATTTTGAACAACAAACCGGAATGGAAATTTATCAGCCCGGTACTAAAACGCTCGGCAAAGTCGCGATTATTGGCGCAGGTCCTGCCGGATTGCAGGCCAGTGTGACACTGACAAACCAGGGTTATGACGTCACGATTTATGAGAAAGAAGCGCACCCCGGTGGCTGGTTGCGTAACGGTATTCCGCAATTCCGGTTACCGCAATCAGTGCTGGATGCAGAGATCGCCCGTATT  >  NC_000913/2234313‑2234584

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑