Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,301,107	T→C	V549V (GTA→GTG)	napA ←	periplasmic nitrate reductase subunit NapA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,301,107	0	T	C	100.0%	161.8 / NA	48	V549V (GTA→GTG)	napA	periplasmic nitrate reductase subunit NapA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (18/30);  total (18/30)

AGCACTTCGTACAGCGTTTTGCCACGCAGTTCCGGTTTCTTCGCCAGCAGATCTTCCGGCCATACTTCTTCAGTTTTGAAGCGGCGGGAGAACTGGACTAACTGCCAGAGATCCGATTTCGCTTCGCCCGGTGCCTGTACCTGTTGACGCCAGAACTGAGTACGGCGTTCGGCGTTACCGTAAGCGCCCTCTTTCTCTACCCACATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGAC  >  NC_000913/2300970‑2301235                                                                                                                                          |                                                                                                                                 aGCACTTCGTACAGCGTTTTGCCACGCAGTTCCGGTTTCTTCGCCAGCAGATCTTCCGGCCATACTTCTTCAGTTTTGAAGCGGCGGGAGAACTGGACTAACTGCCAGAGATCCGATTTCGCTTCGCCCGGTGCCTGCAc                                                                                                                                <  1:206962/140‑1 (MQ=255)    aCTTCGTACAGCGTTTTGCCACGCAGTTCCGGTTTCTTCGCCAGCAGATCTTCCGGCCATACTTCTTCAGTTTTGAAGCGGCGGGAGAACTGGACTAACTGCCAGAGATCCGATTTCGCTTCCCCCGGGGCCTGCACCTg                                                                                                                             >  2:736891/1‑140 (MQ=255)     cTTCGTACAGCGTTTTGCCACGCAGTTCCGGTTTCTTCGCCAGCAGATCTTCCGGCCATACTTCTTCAGTTTTGAAGCGGCGGGAGAACTGGACTAACTGCCAGAGATCCGATTTCGCTTCGCCCGGTGCCTGCACCTGt                                                                                                                            >  2:461203/1‑140 (MQ=255)      ttCGTACAGCGTTTTGCCACGCAGTTCCGGTTTCTTCGCCAGCAGATCTTCCGGCCATACTTCTTCAGTTTTGAAGCGGCGGGAGAACTGGACTAACTGCCAGAGATCCGATTTCGCTTCGCCCGGTGCCTGCACCTGtt                                                                                                                           >  1:462526/1‑140 (MQ=255)      ttCGTACAGCGTTTTGCCACGCAGTTCCGGTTTCTTCGCCAGCAGATCTTCCGGCCATACTTCTTCAGTTTTGAAGCGGCGGGAGAACTGGACTAACTGCCAGAGATCCGATTTCGCTTCGCCCGGTGCCTGCACCTGtt                                                                                                                           <  2:551934/140‑1 (MQ=255)        cGTACAGCGTTTTGCCACGCAGTTCCGGTTTCTTCGCCAGCAGATCTTCCGGCCATACTTCTTCAGTTTTGAAGCGGCGGGAGAACTGGACTAACTGCCAGAGATCCGATTTCGCTTCGCCCGGTGCCTGCACCTGTTGa                                                                                                                         >  2:645500/1‑140 (MQ=255)        cGTACAGCGTTTTGCCACGCAGTTCCGGTTTCTTCGCCAGCAGATCTTCCGGCCATACTTCTTCAGTTTTGAAGCGGCGGGAGAACTGGACTAACTGCCAGACATCCGATATCGCTTCGCCCGGCGCCTGCACCTGTTGa                                                                                                                         >  1:306165/1‑140 (MQ=255)        cGTACAGCGTTTTGCCACGCAGTTCCGGTTTCTTCGCCAGCAGATCTTCCGGCCATACTTCTTCAGTTTTGAAGCGGCGGGAGAACTGGACTAACTGCCACAGAGCCGATTTCGCTTTGCCCGCGGCCCGCGCCTGTTGa                                                                                                                         >  2:409367/1‑140 (MQ=255)           aCAGCGTTTTGCCACGCAGTTCCGGTTTCTTCGCCAGCAGATCTTCCGGCCATACTTCTTCAGTTTTGAAGCGGCGGGAGAACTGGACTAACTGCCAGAGTTCCGATTTCGCTTCGCCCGGTGCCTGCACCTGTTGACGc                                                                                                                      <  2:714629/140‑1 (MQ=255)           aCAGCGTTTTGCCACGCAGTTCCGGTTTCTTCGCCAGCAGATCTTCCGGCCATACTTCTTCAGTTTTGAAGCGGCGGGAGAACTGGACTAACTGCCAGAGATCCGATTTCGCTTCGCCCGGTGCCTGCACCTGTTGACGc                                                                                                                      <  1:218431/140‑1 (MQ=255)               cGTTTTGCCACGCAGTTCCGGTTTCTTCGCCAGCAGATCTTCCGGCCATACTTCTTCAGTTTTGAAGCGGCGGGAGAACTGGACTAACTGCCAGAGATCCGATTTCGCTTCGCCCGGTGCCTGCACCTGTTGACGCCAGa                                                                                                                  >  1:488535/1‑140 (MQ=255)               cGTTTTGCCACGCAGTTCCGGTCTCTTCGCCAGCAGACCTTCCGGCCATACTTCTTCAGTTTTGAAGCGGCGGGAGAACTGGACTAACTGCCAGAGATCCGATTTCGCTTCGCCCGGTGCCTGCACCTGTTGACGCCAGa                                                                                                                  <  1:269847/140‑1 (MQ=255)                       cACGCAGTTCCGGTTTCTTCGCCAGCAGATCTTCCGGCCATACTTCTTCAGTTTTGAAGCGGCGGGAGAACTGGACTAACTGCCAGAGATCCGATTTCGCTTCGCCCGGTGCCTGCACCTGTTGACGCCAGAACTGAGTa                                                                                                          <  2:32708/140‑1 (MQ=255)                                  ggTTTCTTCGCCAGCAGCTCTTCCGGCCATACTTCATCACTTTTGAAGCGGCGGGAGAACTGGACTAACTGCCAGAGATCCGATTTCGCTTCGCCCGATGCCTGCACCTGTTGACGCCAGAACTGAGTAcggcgttcggc                                                                                               <  2:303602/140‑1 (MQ=255)                                      tcttcGCCAGCAGATCTTCCGGCCATACTTCTTCAGTTTTGAAGCGGCGGGAGAACTGGACTAACTGCCAGAGATCCGATTTCGCTTCGCCCGGTGCCTGCACCTGTTGAAGCCAGAACTGAGTACGGCGTTCGGCGTTa                                                                                           >  1:277255/1‑140 (MQ=255)                                          cGCCAGCAGATCTTCCGGCCATACTTCTTCAGTTTTGAAGCGGCGGGAGAACTGGACTAACTGCCAGAGATCCGATTTCGCTTCGCCCGGTGCCTGCACCTGTTGACGCCAGAACTGAGTACGGCGTTCGGCGTTACCGt                                                                                       >  1:607568/1‑140 (MQ=255)                                             cagcagATCTTCCGGCCATACTTCTTCAGTTTTGAAGCGGCGGGAGAAATGGACTAACTGCCAGAGATCCGATTTCGCTTCGCCCGGTGCCCGCACCTGTTTACGCCAGAACTGAGGACGGCGGTCGGCGTTACCGTaaa                                                                                    >  2:851485/1‑139 (MQ=255)                                               gcagATCTTCCGGCCATACTTCTTCAGTTTTGAAGCGGCGGGAGAACTGGACTAACTGCCAGAGATCCGATTTCGCTTCGCCCGGTGCCTGCACCTGTTGACGCCAGAACTGAGTACGGCGTTCGGCGTTACCGTAAgcg                                                                                  <  2:158508/140‑1 (MQ=255)                                               gcagATCTTCCGGCCATACTTCTTCAGTTTTGAAGCGGCGGGAGAACTGGACTAACTGCCAGAGATCCGATTTCGCTTCGCCCGGTGCCTGCACCTGTTGACGCCAGAACTGAGTACGGCGTTCGGCGTTACCGTAAgcg                                                                                  <  1:691942/140‑1 (MQ=255)                                                cagATCTTCCGGCCATACTTCTTCAGTTTTGAAGCGGCGGGAGAACTGGACTAACTGCCAGAGATCCGATTTCGCTTCGCCCGGTGCCTGCACCTGTTGACGCCAGAACTGAGTACGGCGTTCGGCGTTACCGTAAgcgc                                                                                 >  1:134586/1‑140 (MQ=255)                                                  agtCTTCCGGCCATACTTCTTCTGTTTTGAAGCGGCGTGAGAACTGGACTAACTGCCAGAGATCCGATTTCGCTTCGCCCGGTGCCTGCACCTGTTGACGCCAGAACTGAGTACGGCGTTCGGCGTTACCGTAAGCGccc                                                                               <  1:375726/138‑1 (MQ=255)                                                    tCTTCCGGCCATACTTCTTCAGTTTTGAAGCGGCGGGAGAACTGGACTAACTGCCAGAGATCCGATTTCGCTTCGCCCGGTGCCTGCACCTGTTGACGCCAGAACTGAGTACGGCGTTCGGCGTTACCGTAAGCGCCctc                                                                             <  2:777754/140‑1 (MQ=255)                                                    tCTTCCGGCCATACTTCTTCAGTTTTGAAGCGGCGGGAGAACTGGAATAACTGCCAGAGATCCGATTTCGCTTCGCCCGGTGCCTGCACCTGTTGACGCCAGAACTGAGTACGGCGTTCGGCGTTACCGTAAGCGCCctc                                                                             <  2:46647/140‑1 (MQ=255)                                                      ttCCGGCCATACTTCTTCAGTTTTGAAGCGGCGGGAGAACTGGACTAACTGCCAGAGATCCGATTTCGCTTCGCCCGGTGCCTGCACCTGTTGACGCCAGAACTGAGTACGGCGTTCGGCGTTACCGTAAGCGCCCTCtt                                                                           <  1:817867/140‑1 (MQ=255)                                                        ccGGCCATACTTCTTCAGTTTTGAAGCGGCGGGAGAACTGGACTAACTGCCAGAGATCCGATTTCGCTTCGCCCGGTGCCTGCACCTGTTGACGCCAGAACTGAGTACGGCGTTCGGCGTTACCGTAAGCGCCCTCtttt                                                                         >  2:426368/1‑139 (MQ=255)                                                          ggCCATACTTCTTCAGTTTTGAAGCGGCGGGAGAACTGGACTAACTGCCAGAGATCCGATTTCGCTTCGCCCGGTGCCTGCACCTGTTGACGCCAGAACTGAGTACGGCGTTCGGCGTTACCGTAAGCGCCCTCTTtctc                                                                       <  2:607568/140‑1 (MQ=255)                                                          ggCCATACTTCTTCACTTTTGATGCTGCGTGAGAACTCGACTAACTGCCAGAGATCCGATTTCGCTTCGCCCGGTGCCTGCACCTGTTGACGCCAGAACTGAGTACGGCGTTCGGCGTTACCGTAAGCGCCCTCTTtctc                                                                       <  1:406189/140‑1 (MQ=255)                                                          ggCCATACTTCTTCACTTTTGATGCTGCGAGAGAACTGGACTAACTGCCAGAGATCCGATTTCGCTTCGCCCGGTGCCTGCACCTGTTGACGCCAGAACTGAGTACGGCGTTCGGCGTTACCGTAAGCGCCCTCTTtctc                                                                       <  1:409367/140‑1 (MQ=255)                                                           gCCATACTTCTTCAGTTTTGAAGCGGCGGGAGAACTGGACTAACTGCCAGAGATCCGATTTCGCTTCGCCCGGTGCCTGCACCTGTTGACGCCAGAACTGAGTACGGCGTTCGGCGTTACCGTAAGCGCCCTCTTTctct                                                                      <  2:488535/140‑1 (MQ=255)                                                               tACTTCTTCAGTTTTGAAGCGGCGGGAGAACTGGACTAACTGCCAGAGATCCGATTTCGCTTCGCCCGGTGCCTGCACCTGTTGACGCCAGAACTGAGTAGGGCGTTCTGCGTTACCCTAAGGGCCCTCTTTCTCTccct                                                                  >  2:375726/1‑139 (MQ=255)                                                                     ttcAGTTTTGAAGCGGCGGGAGAACTGGACTAACTGCCAGAGATCCGATTTCGCTTCGCCCGGTGCCTGCACCTGTTGACGCCAGAACTGAGTACGGCGTTCGGCGTTACCGTAAGCGCCCTCTTTCTCTACCCACATTg                                                            >  1:460797/1‑140 (MQ=255)                                                                       cAGTTTTGAAGCGGCGGGAGAACTGGACTAACTGCCAGAGATCCGATTTCGCTTCGCCCGGTGCCTGCACCTGTTGACGCCAGAACTGAGTACGGCGTTCGGCGTTACCGTAAGCGCCCTCTTTCTCTACCCACATTGCg                                                          <  2:462526/140‑1 (MQ=255)                                                                       cAGTTTTGAAGCGGCGGGAGAACTGGACTAACTGCCAGAGATCCGATTTCGCTTCGCCCGGTGCCTGCACCTGTTGACGCCAGAACTGAGTACGGCGTTCGGCGTTACCGTAAGCGCCCTCTTTCTCTACCCACATTGCg                                                          <  1:645500/140‑1 (MQ=255)                                                                          ttttGAAGCGGCGGGAGAACTGGACTAACTGCCAGAGATCCGATTTCGCTTCGCCCGGTGCCTGCACCTGTTGACGCCAGAACTGAGTACGGCGTTCGGCGTTACCGTAAGCGCCCTCTTTCTCTACCCACATTGCGGTc                                                       >  1:573065/1‑140 (MQ=255)                                                                            ttGAAGCGGCGGGAGAACTGGACTAACTGCCAGAGATCCGATTTCGCTTCGCCCGGTGCCTGCACCTGTTGACGCCAGAACTGAGTACGGCGTTCGGGGTTACCGTAAGCGCCCTCTTTCTCTACCCACATTGCGGTCgg                                                     >  1:100734/1‑140 (MQ=255)                                                                                         agaACTGGACTAACTGCCAGAGATCCGATTTCGCTTCGCCCGGTGCCTGCACCTGTTGACGCCAGAACTGAGTACGGCGTTCGGCGTTACCGTAAGCGCCCTCTTTCTCTACCCACATTGCGGTCGGCAGGATCAAGTcg                                        <  2:560030/140‑1 (MQ=255)                                                                                         agaACTGGACTAACTGCCAGAGATCCGATTTCGCTTCGCCCGGTGCCTGCACCTGTTGACGCCAGAACTGAGTACGGCGTTCGGCGTTACCGTAAGCGCCCTCTTTCTCTACCCACATTGCGGTCGGCAGGATCAAGTcg                                        <  1:461203/140‑1 (MQ=255)                                                                                             cTGGGCTAACTGCCAGAGATCCGGTTTTGCTTCGCCCGGTGCCTGCACCTGTTGACGCCAGAACTGAGTACGGCGTTCGGCGTTACCGTAAGCGCCCTCTTTCTCTACCCACATTGCGGTCGGCAGGATCAAGTcggcgg                                    >  1:749418/1‑140 (MQ=255)                                                                                              tGGACTAACTGCCAGAGATCCGATTTCGCTTCGCCCGGTGCCTGCACCTGTTGACGCCAGAACTGAGTACGGCGTTCGGCGTTACCGTAAGCGCCCTCTTTCTCTACCCACATTGCGGTCGGCAGGATCAAGTCggcggc                                   <  2:573065/140‑1 (MQ=255)                                                                                                  cTAACTGCCAGAGATCCGATTTTGCTTCGCCAGGTTCCTCCACCTGTTGACGCCAGAACTGAGTACGGCGTTCGGCGTTACCGTAAGCGCCCTCTTTCTCTACCCACATTGCGGTCGGCAGGATCAAGTCGGCGGCCAgc                               <  2:306165/140‑1 (MQ=255)                                                                                                    aaCTGCCAGAGATCCGATTTCGCTTCGCCCGGTGCCTGCACCTGTTGACGCCAGAACTGAGTACGGCGTTCGGCGTTACCGTAAGCGCCCTCTTTCTCTACCCACATTGCGGTCGGCAGGATCAAGTCGGCGGCCAgcgc                             <  2:100734/140‑1 (MQ=255)                                                                                                     aCTGCCAGAGATCCGATTTCGCTTCGCCCGGTGCCTGCACCTGTTGACGCCAGAACTGAGTACGGCGTTCGGCGTTACCGTAAGCGCCCTCTTTCTCTACCCCCATTGCGTTCGCCAGGATCAACTCGGCGGCCAGCGCa                            >  1:343034/1‑140 (MQ=255)                                                                                                     aCTGCCAGAGATCCGATTTCACTTCGCCCGGTGCCTGCACCTGTTGACGCCAGAACTGAGTACGGCGTTCGGCGTTACCGTAAGCGCCCTCTTTCTCTACCCGCATTGCGTTCGTCAGGATCAAGTCGGCGGCCAGCGCa                            >  1:295833/1‑140 (MQ=255)                                                                                                         ccAGAGATCCGATTTCGCTTCGCCCGGTGCCTGCACCTGTTGACGCCAGAACTGAGTACGGCGTTCGGCGTTACCGTAAGCGCCCTCTTTCTCTACCCACATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCactga                        <  2:43622/140‑1 (MQ=255)                                                                                                         ccAGAGATCCGATTTCGCTTCGCCCGGTGCCTGCACCTGTTGACGCCAGAACTGAGTACGGCGTTCGGCGTTACCGTAAGCGCCCTCTTTCTCTACCCACATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCactga                        <  1:851485/140‑1 (MQ=255)                                                                                                         ccAGAGATCCGATTTCGCTTCGCCCGGTGCCTGCACCTGTTGACGCCAGAACTGAGTACGGCGTTCGGCGTTACCGTAAGCGCCCTCTTTCTCTACCCACATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCactga                        <  1:631826/140‑1 (MQ=255)                                                                                                          cAGAGATCCGATTTCGCTTCGCCCGGTGCCTGCACCTGTTGACGCCAGAACTGAGTACGGCGTTCGGCGTTACCGTAAGCGCCCTCTTTCTCTACCCACATTGCGGTCGGCAGGATCAAGTCGGCGGcc                                  <  2:717133/129‑1 (MQ=255)                                                                                                          cAGAGATCCGATTTCGCTTCGCCCGGTGCCTGCACCTGTTGACGCCAGAACTGAGTACGGCGTTCGGCGTTACCGTAAGCGCCCTCTTTCTCTACCCACATTGCGGTCGGCAGGATCAAGTCGGCGGcc                                  >  1:717133/1‑129 (MQ=255)                                                                                                            gagaTCCGATTTCGCTTCGCCCGGTGCCTGCACCTGTTGACGCCAGAACTGAGTACGGCGTTCGGCGTTACCGTAAGCGCCCTCTTTCTCTACCCACATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCactgactg                     <  1:803887/140‑1 (MQ=255)                                                                                                               aTCCGATTTCGCTTCGCCCGGTGCCTGCACCTGTTGACGCCAGAACTGAGTACGGCGTTCGGCGTTACCGTAAGCGCCCTCTTTCTCTACCCACATTGCGCTCGACAGAATCAAGTCAGCGGTCAGCGCACTGACTGTCg                  >  2:271579/1‑140 (MQ=255)                                                                                                                 ccGATTTCGCTTCGCCCGGTGCCTGCACCTGTTGACGCCAGAACTGAGTACGGCGTTCGGCGTTACCGTAAGCGCCCTCTTTCTCTACCCACATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGa                >  2:168857/1‑140 (MQ=255)                                                                                                                       tcgcttcgcCCGGTGCCTGCACCTGTTGACGCCAGAACTGAGTACGGCGTTCGGCGTTACCGTAAGCGCCCTCTTTCTCTACCCACATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACggg          >  2:523641/1‑139 (MQ=255)                                                                                                                         gcttcgcCCGGTGCCTGCACCTGTTGACGCCAGAACTGAGTACGGCGTTCGGCGTTACCGTAAGCGCCCTCTTTCTCTACCCACATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATc        >  2:836482/1‑140 (MQ=255)                                                                                                                               cccGGTGCCTGCACCTGTTGACGCCAGAACTGAGTACGGCGTTCGGCGTTACCGTAAGCGCCCTCTTTCTCTACCCACATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGAc  <  2:749418/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                 AGCACTTCGTACAGCGTTTTGCCACGCAGTTCCGGTTTCTTCGCCAGCAGATCTTCCGGCCATACTTCTTCAGTTTTGAAGCGGCGGGAGAACTGGACTAACTGCCAGAGATCCGATTTCGCTTCGCCCGGTGCCTGTACCTGTTGACGCCAGAACTGAGTACGGCGTTCGGCGTTACCGTAAGCGCCCTCTTTCTCTACCCACATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGAC  >  NC_000913/2300970‑2301235

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑