Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,312,343	T→C	Y136C (TAC→TGC)	ompC ←	outer membrane porin C

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,312,343	0	T	C	100.0%	134.8 / NA	39	Y136C (TAC→TGC)	ompC	outer membrane porin C
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (19/20);  total (19/20)

CTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAGTTGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACGGGTCCA  >  NC_000913/2312209‑2312473                                                                                                                                       |                                                                                                                                   cTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTGACCACGCTGCTACACGAAGTTATCAGAACCGCAGgtg                                                                                                                               >  1:387212/1‑140 (MQ=255)    cACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAGTTGTCAGAACCGCAGGTGTca                                                                                                                            >  2:430726/1‑140 (MQ=255)    cACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAGTTGTCAGAACCGCAGGTGTca                                                                                                                            >  1:595300/1‑140 (MQ=255)    cACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAGTTGTCAGAACCGCAGGTGTca                                                                                                                            <  1:500870/140‑1 (MQ=255)    cACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGGTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGCAGGTCGCGAAGCCGTTACCACGCTGCTGCGGGAAGGTGTCAGAACCGCAGGGGTca                                                                                                                            >  2:803313/1‑140 (MQ=255)        aGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAGTTGTCAGAACCGCAGGTGTcaccac                                                                                                                        <  1:120721/140‑1 (MQ=255)          aTGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAGTTGTCAGAACCGCAGGTGTCACCACCg                                                                                                                      >  1:20550/1‑140 (MQ=255)          aTGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAGTTGTCAGAACCGCAGGTGTCACCACCg                                                                                                                      >  1:660079/1‑140 (MQ=255)            gggTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAGTTGTCAGAACCGCAGGTGTCACCACCGaa                                                                                                                    <  2:595300/140‑1 (MQ=255)            gggTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAGTTGTCAGAACCGCAGGTGTCACCACCGaa                                                                                                                    >  1:797871/1‑140 (MQ=255)             ggTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAGTTGTCAGAACCGCAGGTGTCACCACCGAAt                                                                                                                   <  1:656479/140‑1 (MQ=255)                    gTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAGTTGTCAGAACCGCAGGTGTCACCACCGAATTc                                                                                                                 <  1:436945/135‑1 (MQ=255)                    gTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAGTTGTCAGAACCGCAGGTGTCACCACCGAATTc                                                                                                                 >  2:436945/1‑135 (MQ=255)                           cccTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAGTTGTCAGAACCGCAGGTGTCACCACCGAATTCTGGCAGTACGTc                                                                                                     <  2:660079/140‑1 (MQ=255)                                 tACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAGTTGTCAGAACCGCAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCa                                                                                               >  1:435098/1‑140 (MQ=255)                                      aaCAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAGTTGTCAGAACCGCAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAg                                                                                          >  2:183682/1‑140 (MQ=255)                                      aaCAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAGTTGTCAGAACCGCAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAg                                                                                          >  1:818757/1‑140 (MQ=255)                                        cAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAGTTGTCAGAACCGCAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGtt                                                                                        <  2:797871/140‑1 (MQ=255)                                         aGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAGTTGTCAGAACCGCAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTa                                                                                       <  1:430726/140‑1 (MQ=255)                                           cAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAGTTGTCAGAACCGCAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACg                                                                                     >  1:766465/1‑140 (MQ=255)                                                     gCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAGTTGTCAGAACCGCAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAaacaa                                                                           <  1:803313/140‑1 (MQ=255)                                                         tCAACCAGACCGAAGACATCAGTGTTACGGTGGTTGGCGCAGCCGTTATCACGCTGCTGCATGAAGTTGTCAGAACCGCAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGcc                                                                       <  2:387212/140‑1 (MQ=255)                                                         tCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAGTTGTCAGAACCGCAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGcc                                                                       <  2:435098/140‑1 (MQ=255)                                                          cAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAGTTGTCAGAACCGCAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCg                                                                      >  1:691706/1‑140 (MQ=255)                                                                     aagaagTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAGTTGTCAGAACCGCAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGAcc                                                           <  1:61114/140‑1 (MQ=255)                                                                                       tAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAGTTGTCAGAACCGCAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGt                                                      <  1:584395/127‑1 (MQ=255)                                                                                       tAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAGTTGTCAGAACCGCAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGt                                                      >  2:584395/1‑127 (MQ=255)                                                                                         ggTCGCGAAGCCGTTACCACGCTGCTGCATGAAGTTGTCAGAACCGCAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACGACGCCGTGGTTTCGGCCGTAGTCGAAAGAACCCACAt                                       >  1:414229/1‑140 (MQ=255)                                                                                         ggTCGCGAAGCCGTTACCACGCTGCTGCATGAAGTTGTCAGAACCGCAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACAt                                       <  1:87455/140‑1 (MQ=255)                                                                                         ggTCGCGAAGCCGTTACCACGCTGCTGCATGAAGTTGTCAGAACCGCAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACAt                                       >  2:87455/1‑140 (MQ=255)                                                                                         ggTCGCGAAGCCGTTACCACGCTGCTGCATGAAGTTGTCAGAACCGCAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACAt                                       >  2:746597/1‑140 (MQ=255)                                                                                              cgAAGCCGTTACCACGCTGCTGCATGAAGTTGTCAGAACCGCAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTgg                                  <  2:556718/140‑1 (MQ=255)                                                                                                                gctgcATGAAGTTGTCAGAACCGCAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGTATTTCAGACCTGCGAAt                >  2:332379/1‑140 (MQ=255)                                                                                                                     aTGAAGTTATCAGAACAGCAGGCGTCACCATGGAAGTCTCGCAGTACGACGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCcac           <  2:414229/140‑1 (MQ=255)                                                                                                                      tGAAGTTGTCAGAACCGCAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCcaca          >  1:688647/1‑140 (MQ=255)                                                                                                                      tGAAGTTGTCAGAACCGCAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCcaca          <  2:691706/140‑1 (MQ=255)                                                                                                                      tGAAGTTGTCAGAACCGCAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCcaca          <  2:135510/140‑1 (MQ=255)                                                                                                                       gAAGTTGTCAGAACCGCAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCacac         <  2:688647/140‑1 (MQ=255)                                                                                                                        aaGTTGTCAGAACCGCAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACg        <  1:332379/140‑1 (MQ=255)                                                                                                                           ttGTCAGAACCGCAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACGGGt     <  1:746597/140‑1 (MQ=255)                                                                                                                              tCAGAACCGCAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACGGGTCCa  >  1:491877/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                                   CTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAGTTGTCAGAACCGTAGGTGTCACCACCGAATTCTGGCAGTACGTCGGTCCAGGAAGTTACGTCATAAACAACGCCGTAGTTACGACCGTAGTCGAAAGAACCCACATCCTGGAATTTCAGACCTGCGAATGCCACACGGGTCCA  >  NC_000913/2312209‑2312473

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑