Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,362,013	T→C	T67A (ACC→GCC)	yfaX ←	putative DNA‑binding transcriptional regulator YfaX

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,362,013	0	T	C	100.0%	106.8 / NA	32	T67A (ACC→GCC)	yfaX	putative DNA‑binding transcriptional regulator YfaX
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (18/14);  total (18/14)

ATCAATAATCCCCAGGTGACATAACAGCCCGGTGGTATCCATCAGTTGCGTCAAACGCGGTCGAGCCAGTTCCCGGAGATCCATCTTGCTTAACGCATGACCGGAAAGCTCCACCAGCCTGGTCCACAAACAAAAATTTTCCTGGTGATCAAGGCTAAGAAAACGCTGACGCCTGAGTTCATTAAGCAGCAAATAGGCCGTGCTTTTAGGGATTCCCAATGTGTCAATGATCGTCGCAGCACTACAGGGGCCAATGCG  >  NC_000913/2361891‑2362148                                                                                                                           |                                                                                                                                        aTCAATAATCCCCAGGTGACATAACAGCCCGGTGGTATCCATCAGTTGCGTCAAACGCGGTCGAGCCAGTTCCCGGAGATCCATCTTGCTTAACGCATGACCGGAAAGCTCCACCAGCCTGGCCCACAAACAAAAAtttt                                                                                                                        >  2:26856/1‑140 (MQ=255)          ccccAGGTGACATAACAGCCCGGTGGTATCCATCAGTTGCGTCAAACGCGGTCGAGCCAGTTCCCGGAGATCCATCTTGCTTAACGCATGACCGGAAAGCTCCACCAGCCTGGCCCACAAACAAAAATTTTCCTGGTGAt                                                                                                               <  2:144954/140‑1 (MQ=255)            ccAGGTGACATAACAGCCCGGTGGTATCCATCAGTTGCGTCAAACGCGGTCGAGCCAGTTCCCGGAGATCCATCTTGCTTAACGCATGACCGGAAAGCTCCACCAGCCTGGCCCACAAACAAAAATTTTCCTGGTGATCa                                                                                                             >  1:787627/1‑140 (MQ=255)            ccAGGTGACATAACAGCCCGGTGGTATCCATCAGTTGCGTCAAACGCGGTCGAGCCAGTTCCCGGAGATCCATCTTGCTTAACGCATGACCCGAAAGCTCAACCGGCCGGGCCCACAAACAAAAATTTTCCTGGTGATCa                                                                                                             >  2:252491/1‑140 (MQ=255)                gTGACATAACAGCCCGGTGGTATCCATCAGTTGCGTCAAACGCGGTCGAGCCAGTTCCCGGAGATCCATCTTGCTTAACGCATGACCGGAAAGCTGCACCAGCCTGGCCCACAAACAAAAATTTTCCTGGTGATCAAGGc                                                                                                         >  1:537946/1‑140 (MQ=255)                             ccGGTGGTATCCATCAGTTGCGTCAAACGCGGTCGAGCCAGTTCCCGGAGATCCATCTTGCTTAACGCATGACCGGAAAGCTCCACCAGCCTGGCCCACAAACAAAAATTTTCCTGGTGATCAAGGCTAAGAAAACGCTg                                                                                            >  2:117938/1‑140 (MQ=255)                             ccGGTGGTATCCATCAGTTGCGTCAAACGCGGTCGAGCCAGTTCCCGGAGATCCATCTTGCTTAACGCATGACCGGAAAGCTCCACCAGCCCGGCCCACAAACAAAAAGTTTCCTGGGGATCTAGGCTAAGAAAACGCTg                                                                                            >  2:383250/1‑140 (MQ=255)                              cGGTGGTATCCATCAGTTGCGTCAAACGCGGTCGAGCCAGTTCCCGGAGATCCATCTTGCTTAACGCATGACCGGAAAGCTCCACCAGCCTGGCCCACAAACAAAAATTTTCCTGGTGATCAAGGCTAAGAAAACGCTGa                                                                                           >  1:596233/1‑140 (MQ=255)                               ggtggtATCCATCAGTTGCGTCAAACGCGGTCGAGCCAGTTCCCGGAGATCCATCTTGCTTAACGCATGACCGGAAAGCTCCACCAGCCTGGCCCACAAACAAAAATTTTCCTGGTGATCAAGGCTAAGAAAACGCTGAc                                                                                          >  1:479323/1‑140 (MQ=255)                                   gtATCCATCAGTTGCGTCAAACGCGGTCGAGCCAGTTCCCGGAGATCCATCTTGCTTAACGCATGACCGGAAAGCTCCACCAGCCTGGCCCACAAACAAAAATTTTCCTGGTGATCAAGGCTAAGAAAACGCTGACGCCt                                                                                      <  1:26856/140‑1 (MQ=255)                                     aTCCATCAGTTGCGTCAAACGCGGTGGAGGCAGTTCCCGGAGATCCATCTTGCTTAACGCATGACCGGAAAGCTCCACCAGCCTGGCCCACAAACAAAAATTTTCCTGGTGATCAAGGCTAAGAAAACGCTGACGCCTGa                                                                                    <  1:383250/140‑1 (MQ=255)                                      tCCATCAGTTGCGTCAAACGCGGTCGAGCCAGTTCCCGGAGATCCATCTTGCTTAACGCATGACCGGAAAGCTCCACCAGCCTGGCCCACAAACAAAAATTTTCCTGGTGATCAAGGCTAAGAAAACGCTGACGCCTGAg                                                                                   >  1:773158/1‑140 (MQ=255)                                                     aaaCGCGGTCGAGCCAGTTCCCGGAGATCCATCTTGCTTAACGCATGACCGGAAAGCTCCACCAGCCTGGCCCACAAACAAAAATTTTCCTGGTGATCAAGGCTAAGAAAACGCTGACGCCTGAGTTCATTAAGCAGCaa                                                                    >  1:12909/1‑140 (MQ=255)                                                        cgcgGTCGAGCCAGTTCCCGGAGATCCATCTTGCTTAACGCATGACCGGAAAGCTCCACCAGCCTGGCCCACAAACAAAAATTTTCCTGGTGATCAAGGCTAAGAAAACGCTGACGCCTGAGTTCATTAAGCAGCAAATa                                                                 >  1:732480/1‑140 (MQ=255)                                                           ggTCGAGCCAGTTCCCGGAGATCCATCTTGCTTAACGCATGACCGGAAAGCTCCACCAGCCTGGCCCACAAACAAAAATTTTCCTGGTGATCAAGGCTAAGAAAACGCTGACGCCTGAGTTCATTAAGCAGCAAATAGGc                                                              <  2:773158/140‑1 (MQ=255)                                                             tCGAGCCAGTTCCCGGAGATCCATCTTGCTTAACGCATGACCGGAAAGCTCCACCAGCCTGGCCCACAAACAAAAATTTTCCTGGTGATCAAGGCTAAGAAAACGCTGACGCCTGAGTTCATTAAGCAGCAAATAGGCCg                                                            <  2:732480/140‑1 (MQ=255)                                                             tCGAGCCAGTTCCCGGAGATCCATCTTGCTTAACGCATGACCGGAAAGCTCCACCAGCCTGGCCCACAAACAAAAATTTTCCTGGTGATCAAGGCTAAGAAAACGCTGACGCCTGAGTTCATTAAGCAGCAAATAGGCCg                                                            <  1:117938/140‑1 (MQ=255)                                                                           ggAGATCCATCTTGCTTAACGCATGACCGGAAAGCTCCACCAGCCTGGCCCACAAACAAAAATTTTCCTGGTGATCAAGGCTAAGAAAACGCTGACGCCTGAGTTCATTAAGCAGCAAATAGGCCGTGCTTTTAGGGAtt                                              >  1:691951/1‑140 (MQ=255)                                                                             agaTCCATCTTGCTTAACGCATGACCGGAAAGCTCCACCAGCCTGGCCCACAAACAAAAATTTTCCTGGTGATCAAGGCTAAGAAAACGCTGACGCCTGAGTTCATTAAGCAGCAAATAGGCCGTGCTTTTAGGGATTcc                                            <  1:806687/140‑1 (MQ=255)                                                                               aTCCATCTTGCTTAACGCATGACCGGAAAGCTCCACCAGCCTGGCCCACAAACAAAAATTTTCCTGGTGATCAAGGCTAAGAAAACGCTGACGCCTGAGTTCATTAAGCAGCAAATAGGCCGTGCTTGTAGGGATTCCCa                                          <  2:596233/140‑1 (MQ=255)                                                                                tCCATCTTGCTTAACGCATGACCGGAAAGCTCCACCAGCCTGGCCCACAAACAAAAATTTTCCTGGTGATCAAGGCTAAGAAAACGCTGACGCCTGAGTTCATTAAGCAGCAAATAGGCCGTGCTTTTAGGGATTCCCaa                                         >  1:490176/1‑140 (MQ=255)                                                                                tCCATCTTGCTTAACGCATGACCGGAAAGCTCCACCAGCCTGGCCCACAAACAAAAATTTTCCTGGTGATCAAGGCTAAGAAAACGCTGACGCCTGAGTTCATTAAGCAGCAAATAGGCCGTGCTTTTAGGGATTCCCaa                                         <  2:787627/140‑1 (MQ=255)                                                                                 ccATCTTGCTTAACGCATGACCTGATAGCACCACCAGGCTGGCCCACAAACAAAAATTTTCCTGGTGATCAAGGCTAAGAAAACGCTGACGCCTGAGTTCATTAAGCAGCAAATAGGCCGTGCTTTTAGGGATTCCCAAt                                        <  1:252491/140‑1 (MQ=255)                                                                                            aaCGCATGACCGGAAAGCTCCACCAGCCTGGCCCACAAACAAAAATTTTCCTGGTGATCAAGGCTAAGAAAACGCTGACGCCTGAGTTCATTAAGCAGCAAATAGGCCGTGCTTTTAGGGATTCCCAATGTGTCAATGAt                             <  2:479323/140‑1 (MQ=255)                                                                                            aaCGCATGACCGGAAAGCTCCACCAGCCTGGCCCACAAACAAAAATTTTCCTGGTGATCAAGGCTAAGAAAACGCTGACGCCTGAGTTCATTAAGCAGCAAATAGGCCGTGCTTTTAGGGATTCCCAATGTGTCAATGAt                             <  2:691951/140‑1 (MQ=255)                                                                                                  tGACCGGAAAGCTCCACCAGCCTGGCCCACAAACAAAAATTTTCCTGGTGATCAAGGCTAAGAAAACGCTGACGCCTGAGTTCATTAAGCAGCAAATAGGCCGTGCTTTTAGGGATTCCCAATGTGTCAATGATCGTCgc                       >  1:776633/1‑140 (MQ=255)                                                                                                   gACCGGAAAGCTCCACCAGCCTGGCCCACAAACAAAAATTTTCCTGGTGATCAAGGCTAAGAAAACGCTGACGCCTGAGTTCATTAAGCAGCAAATAGGCCGTGCTTTTAGGGATTCCCAATGTGTCAATGAtcgt                          <  1:560214/136‑1 (MQ=255)                                                                                                   gACCGGAAAGCTCCACCAGCCTGGCCCACAAACAAAAATTTTCCTGGTGATCAAGGCTAAGAAAACGCTGACGCCTGAGTTCATTAAGCAGCAAATAGGCCGTGCTTTTAGGGATTCCCAATGTGTCAATGAtcgt                          >  2:560214/1‑136 (MQ=255)                                                                                                             cTCCACCAGCCTGGCCCACAAACAAAAATTTTCCTGGGGATCAAGGCTAAGAAAACGCTGACGCCTGAGTTCATTAAGCAGCAAATAGGCCGTGCTTTTAGGGATTCCCAATGTGTCAATGATCGTCGCAGCACTACAgg            >  1:103752/1‑140 (MQ=255)                                                                                                              tCCACCAGCCTGGCCCACAAACAAAAATTTTCCTGGTGATCAAGGCTAAGAAAACGCTGACGCCTGAGTTCATTAAGCAGCAAATAGGCCGTGCTTTTAGGGATTCCCAATGTGTCAATGAACGTCGCAGCACTACAggg           >  1:519887/1‑140 (MQ=255)                                                                                                                      ccTGGCCCACAAACAAAAATTTTCCTGGTGATCAAGGCTAAGAAAACGCTGACGCCTGAGTTCATTAAGCAGCAAATAGGCCGTGCTTTTAGGGATTCCCAATGTGTCAATGATCGTCGCAGCACTACAGGGGCCAATGc   >  2:577364/1‑140 (MQ=255)                                                                                                                       cTGGCCCACAAACAAAAATTTTCCTGGTGATCAAGGCTAAGAAAACGCTGACGCCTGAGTTCATTAAGCAGCAAATAGGCCGTGCTTTTAGGGATTCCCAATGTGTCAATGATCGTCGCAGCACTACAGGGGCCAATGCg  <  2:490176/140‑1 (MQ=255)                                                                                                                           |                                                                                                                                        ATCAATAATCCCCAGGTGACATAACAGCCCGGTGGTATCCATCAGTTGCGTCAAACGCGGTCGAGCCAGTTCCCGGAGATCCATCTTGCTTAACGCATGACCGGAAAGCTCCACCAGCCTGGTCCACAAACAAAAATTTTCCTGGTGATCAAGGCTAAGAAAACGCTGACGCCTGAGTTCATTAAGCAGCAAATAGGCCGTGCTTTTAGGGATTCCCAATGTGTCAATGATCGTCGCAGCACTACAGGGGCCAATGCG  >  NC_000913/2361891‑2362148

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑