Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,362,203	T→C	E3E (GAA→GAG)	yfaX ←	putative DNA‑binding transcriptional regulator YfaX

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,362,203	0	T	C	100.0%	101.7 / NA	29	E3E (GAA→GAG)	yfaX	putative DNA‑binding transcriptional regulator YfaX
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (17/12);  total (17/12)

TTCATTAAGCAGCAAATAGGCCGTGCTTTTAGGGATTCCCAATGTGTCAATGATCGTCGCAGCACTACAGGGGCCAATGCGGGCAATCAAATTGAGAATATCAATAGCCCGTGTCAGGGCGGGAACTTTGCTTGATTCCAACATACTGGACTCCAGTCTTAGATACTGGAATCAGTCTCGTTGCTCAGGGGGAGTAAAATTGTGAAGCGCATCAAATCCCGCTCCTCGTCCAGCAGACTGGAGAAATTACGC  >  NC_000913/2362068‑2362319                                                                                                                                        |                                                                                                                     ttCATTAAGCAGCAAATAGGCCGTGCTTTTAGGGATTCCCAATGTGTCAATGATCGTCGCAGCACTACAGGGGCCAATGCGGGCAATCAAATTGAGAATATCAATAGCCCGTGTCAGGGCGGGAACTTTGCTTGACTCCa                                                                                                                  >  1:193763/1‑140 (MQ=255) ttCATTAAGCAGCAAATAGGCCGTGCTTTTAGGGATTCCCAATGTGTCAATGATCGTCGCAGCACTACAGGGGCCAATGCGGGCAATCAAATTGAGAATATCAATAGCCCGTGTCAGGGCGGGAACTTTGCTTGACTCCa                                                                                                                  <  2:479840/140‑1 (MQ=255)    aTTAAGCAGCAAATAGGCCGTGCTTTTAGGGATTCCCAATGTGTCAATGATCGTCGCAGCACTACAGGGGCCAATGCGGGCAATCAAATTGAGAATATCAATAGCCCGTGTCAGGGCGGGAACTTTGCTTGACTCCAACa                                                                                                               <  2:193763/140‑1 (MQ=255)       aaGCAGCAAATAGGCCGTGCTTTTAGGGATTCCCAATGTGTCAATGATCGTCGCAGCACTACAGGGGCCAATGCGGGCAATCAAATTGAGAATATCAATAGCCCGTGTCAGGGCGGGAACTTTGCTTGACTCCAACATAc                                                                                                            <  1:143180/140‑1 (MQ=255)                      cGTGCTTTTAGGGATTCCCAATGTGTCAATGATCGTCGCAGCACTACAGGGGCCAATGCGGGCAATCAAATTGAGAATATCAATAGCCCGTGTCAGGGCGGGAACTTTGCTTGACTCCAACATACTGGACTCCAGTc                                                                                                >  2:783485/1‑137 (MQ=255)                      cGTGCTTTTAGGGATTCCCAATGTGTCAATGATCGTCGCAGCACTACAGGGGCCAATGCGGGCAATCAAATTGAGAATATCAATAGCCCGTGTCAGGGCGGGAACTTTGCTTGACTCCAACATACTGGACTCCAGTc                                                                                                <  1:783485/137‑1 (MQ=255)                       gTGCTTTTAGGGATTCCCAATGTGTCAATGATCGTCGCAGCACTACAGGGGCCAATGCGGGCAATCAAATTGAGAATATCAATAGCCCGTGTCAGGGCGGGAACTTTGCTTGACTCCAACATACTGGACTCCAGTCTTAg                                                                                            >  1:541560/1‑140 (MQ=255)                              tAGGGATTCCCAATGTGTCAATGATCGTCGCAGCACTACAGGGGCCAATGCGGGCAATCAAATTGAGAATATCAATAGCCCGTGTCAGGGCGGGAACTTTGCTTGACTCCAACATACTGGACTc                                                                                                     >  1:207740/1‑124 (MQ=255)                              tAGGGATTCCCAATGTGTCAATGATCGTCGCAGCACTACAGGGGCCAATGCGGGCAATCAAATTGAGAATATCAATAGCCCGTGTCAGGGCGGGAACTTTGCTTGACTCCAACATACTGGACTc                                                                                                     <  2:207740/124‑1 (MQ=255)                               aGGGATTCCCAATGTGTCAATGATCGTCGCAGCACTACAGGGGCCAATGCGGGCAATCAAATTGAGAATATCAATAGCCCGTGTCAGGGCGGGAACTTTGCTTGACTCCAACATACTGGACTCCAGTCTTAGATACTGGa                                                                                    >  2:35473/1‑140 (MQ=255)                                    ttCCCAATGTGTCAATGATCGTCGCAGCACTACAGGGGCCAATGCGGGCAATCAAATTGAGAATATCAATAGCCCGTGTCAGGGCGGGAACTTTGCTTGACTCCAACATACTGGACTCCAGTCTTAg                                                                                            <  2:865789/127‑1 (MQ=255)                                    ttCCCAATGTGTCAATGATCGTCGCAGCACTACAGGGGCCAATGCGGGCAATCAAATTGAGAATATCAATAGCCCGTGTCAGGGCGGGAACTTTGCTTGACTCCAACATACTGGACTCCAGTCTTAg                                                                                            >  1:865789/1‑127 (MQ=255)                                               tCAATGATCGTCGCAGCACTACAGGGGCCAATGCGGGCAATCAAATTGAGAATATCAATAGCCCGTGTCAGGGCGGGAACTTTGCTTGACTCCAACATACTGGACTCCAGTCTTAGATACTGGAATCAGTCTCGTTGCTc                                                                    >  1:17421/1‑140 (MQ=255)                                               tCAATGATCGTCGCAGCACTACAGGGGCCAATGCGGGCAATCAAATTGAGAATATCAATAGCCCGTGTCAGGGCGGGAACTTTGCTTGACTCCAACATACTGGACTCCAGTCTTAGATACTGGAATCAGTCTCGTTGCTc                                                                    >  1:450228/1‑140 (MQ=255)                                                  aTGATCGTCGCAGCACTACAGGGGCCAATGCGGGCAATCAAATTGAGAATATCAATAGCCCGTGTCAGGGCGGGAACTTTGCTTGACTCCAACATACTGGACTCCAGTCTTAGATACTGGAATCAGTCTCGTTGCTCAgg                                                                 <  1:561044/140‑1 (MQ=255)                                                  aTGATCGTCGCAGCACTACAGGGGCCAATGCGGGCAATCAAATTGAGAATATCAATAGCCCGTGTCAGGGCGGGAACTTTGCTTGACTCCAACATACTGGACTCCAGTCTTAGATACTGGAATCAGTCTCGTTGCTCAgg                                                                 <  2:27547/140‑1 (MQ=255)                                                  aTGATCGTCGCAGCACTACAGGGGCCAATGCGGGCAATCAAATTGAGAATATCAATAGCCCGTGTCAGGGCGGGAACTTTGCTTGACTCCAACATACTGGACTCCAGTCTTAGATACTGGAATCAGTCTCGTTGCTCAgg                                                                 <  1:35473/140‑1 (MQ=255)                                                  aTGATCGTCGCAGCACTACAGGGGCCAATGCGGGCAATCAAATTGAGAATATCAATAGCCCGTGTCAGGGCGGGAACTTTGCTTGACTCCAACATACTGGACTCCAGTCTTAGATACTGGAATCAGTCTCGTTGCTCAgg                                                                 <  2:450228/140‑1 (MQ=255)                                                                         gCCAATGCGGGCAATCAAATTGAGAATATCAATAGCCCGTGTCAGGGCGGGAACTTTGCTTGACTCCAACATACTGGACTCCAGTCTTAGATACTGGAATCAGTCTCGTTGCTCAGGGGGAGTAAAATTGTGAAGCGCAt                                          >  1:651154/1‑140 (MQ=255)                                                                         gCCAATGCGGGCAATCAAATTGAGAATATCAATAGCCCGTGTCAGGGCGGGAACTTTGCTTGACTCCAACATACTGGACTCCAGTCTTAGATACTGGAATCAGTCTCGTTGCTCAGGGGGAGTAAAATTGTGAAGCGCAt                                          >  2:130984/1‑140 (MQ=255)                                                                         gCCAATGCGGGCAATCAAATTGAGAATATCAATAGCCCGTGTCAGGGCGGGAACTTTGCTTGACTCCAACATACTGGACTCCAGTCTTAGATACTGGAATCAGTCTCGTTCCTCAGGAGGAGTACAATTGTGAAGCGCAt                                          >  1:291091/1‑140 (MQ=255)                                                                            aaTGCGGGCAATCAAATTGAGAATATCAATAGCCCGTGTCAGGGCGGGAACTTTGCTTGACTCCAACATACTGGACTCCAGTCTTAGATACTGGAATCAGTCTCGTTGCTCAGGGGGAGTAAAATTGTGAAGCGCATCaa                                       >  1:1798/1‑140 (MQ=255)                                                                                           aTTGAGAATATCAATAGCCCGTGTCAGGGCGGGAACTTTGCTTGACTCCAACATACTGGACTCCAGTCTTAGATACTGGAATCAGTCTCGTTGCTCAGGGGGAGTAAAATTGTGAAGCGCATCAAATCCCGCTCCTCGTc                        <  2:519887/140‑1 (MQ=255)                                                                                             tGAGAATATCAATAGCCCGTGTCAGGGCGGGAACTTTGCTTGACTCCAACATACTGGACTCCAGTCTTAGATACTGGAATCAGTCTCGTTGCTCAGGGGGAGTAAAATTGTGAAGCGCATCAAATCCCGCTCCTCGTCca                      >  1:698408/1‑140 (MQ=255)                                                                                                 aaTATCAATAGCCCGTGTCAGGGCGGGAACTTTGCTTGACTCCAACATACTGGACTCCAGTCTTAGATACTGGAATCAGTCTCGTTGCTCAGGGGGAGTAAAATTGTGAAGCGCATCAAATCCCGCTCCTCGTCcagcag                  <  2:698408/140‑1 (MQ=255)                                                                                                              cGTGTCAGGGCGGGAACTTTGCTTGACTCCAACATACTGGACTCCAGTCTTAGATACTGGAATCAGTCTCGTTGCTCAGGGGGAGTAAAATTGTGAAGCGCATCAAATCCTGCTACTCGTCCAGTAGACTGGAGAAATTa     >  1:243751/1‑140 (MQ=255)                                                                                                              cGTGTCAGGGCGGGAACTTTGCTTGACTCCAACATACTGGACTCCAGTCTTAGATACTGGAATCAGTCTCGTTGCTCAGGGGGAGTAAAATTGTGAAGCGCATCAAATCCCGCTCCTCGTCCAGCAGACTGGAGAAATTa     >  2:461606/1‑140 (MQ=255)                                                                                                              cGTGTCAGGGCGGGAACTTTGCTTGACTCCAACATACTGGACTCCAGTCTTAGATACTGGAATCAGTCTCGTTGCTCAGGGGGAGTAAAATTGTGAAGCGCATCAAATCCCGCTCCTCGTCCAGCAGACTGGAGAAATTa     >  2:747038/1‑140 (MQ=255)                                                                                                                tgtCGGGGCGGCAAGTTTGCTTGATTCCAACATCCCTGACTCCCGGGTTAGATACTGGAATCAGGCCCGTTGCTCAGGGGGGGTAAAATTGTGAAGCGCATCAAATCCCGCTCCTCGTCCAGCAGACTGGAGGAATTACg   >  2:803724/1‑140 (MQ=255)                                                                                                                 gtCAGGGCGGGAACTTTGCTTGACTCCAACATACTGGACTCCAGTCTTAGATACTGGAATCAGTCTCGTTGCTCAGGGGGAGTAAAATTGTGAAGCGCATCAAATCCCGCTCCTCGTCCAGCAGACTGGAGAAATTACGc  >  2:572057/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                     TTCATTAAGCAGCAAATAGGCCGTGCTTTTAGGGATTCCCAATGTGTCAATGATCGTCGCAGCACTACAGGGGCCAATGCGGGCAATCAAATTGAGAATATCAATAGCCCGTGTCAGGGCGGGAACTTTGCTTGATTCCAACATACTGGACTCCAGTCTTAGATACTGGAATCAGTCTCGTTGCTCAGGGGGAGTAAAATTGTGAAGCGCATCAAATCCCGCTCCTCGTCCAGCAGACTGGAGAAATTACGC  >  NC_000913/2362068‑2362319

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑