Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,378,828	G→A	P144P (CCC→CCT)	menD ←	2‑succinyl‑5‑enolpyruvyl‑6‑hydroxy‑3‑ cyclohexene‑1‑carboxylate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,378,828	0	G	A	100.0%	86.2 / NA	26	P144P (CCC→CCT)	menD	2‑succinyl‑5‑enolpyruvyl‑6‑hydroxy‑3‑ cyclohexene‑1‑carboxylate synthase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (14/12);  total (14/12)

CCAGACGCTGTTGCCAGCTAAGCCCGGTATCGTCCATTTCGCCATACAGCGGTTCAGCAAACGGGCAGTTGATATGGACTCCCCCCGCATGAAGCGTACCGAGAGCGTGGTCGATGGTAGAAACCAGCCAACGTGCGGGGATATCCTGGGTCGGGCGCGGCAATGAAATACTGTGCGTGGGGTGAGAGGCGAACATTCCCGGCTGGCGAATTGCCTGATTCGCGCCGCAGTCAATTAGCTCCGGCGGGCGATCGGCGGTTAAGAGAA  >  NC_000913/2378692‑2378958                                                                                                                                         |                                                                                                                                   ccAGACGCTGTTGCCAGCTAAGCCCGGTATCGTCCATTTCGCCATACAGCGGTTCAGCAAACGGGCAGTTGATATGGACTCCCCCCGCATGAAGCGTACCGAGAGCGTGGTCGATGGTAGAAACCAGCCAACGCGCaggg                                                                                                                                 <  1:790266/140‑1 (MQ=255)           ttGCCAGCTAAGCCCGGTATCGTCCATTTCGCCATACAGCGGTTCAGCAAACGGGCAGTTGATATGGACTCCCCCCGCATGAAGCGTACCGAGAGCGTGGTCAATGGTAGGAACAAGGCAACGTGCAGGGATATCCTggg                                                                                                                       >  1:244505/1‑140 (MQ=255)                     aGCCCGGTATCGTCCATTTCGCCATACAGCGGTTCAGCAAACGGGCAGTTGATATGGACTCCCCCCGCATGAAGCGTACCGAGAGCGTGGTCGATGGTAGAAACCAGCCAACGTGCAGGGATATCCTGGGTCGGGCGCgg                                                                                                             <  2:776070/140‑1 (MQ=255)                     aGCCCGGTATCGTCCATTTCGCCATACAGCGGTTCAGCAAACGGGCAGTTGATATGGACTCCCCCCGCATGAAGCGTACCGAGAGCGTGGTCGATGGTAGAAACCAGCCAACGTGCAGGGATATCCTGGGTCGGGCGCgg                                                                                                             <  2:60610/140‑1 (MQ=255)                     aGCCCGGTATCGTCCATGTCGCTATACAGCGTTTCCGCATACGGGCAGTTGATATGGACTCCCCCCGCATGAAGCGTACCGAGAGCGTGGTCGATGGTAGAAACCAGCCAACGTGCAGGGATATCCTGGGTCGGGCGCgg                                                                                                             <  2:261023/140‑1 (MQ=255)                     aGCCCGGTATCGTCCATCTCGCCATACAGCGCTTCTGCATACGGGCAGATGATATGGACTCCCCCCGCATGAAGCGTACCGAGAGCGTGGTCGATGGTAGAAACCAGCCAACGTGCAGGGATATCCTGGGTCGGGCGCgg                                                                                                             <  2:244505/140‑1 (MQ=255)                          ggTATCGTCCATTTCGCTATACAGCGGTTCACCAACCGGACAGTTGATATGGACTCCCCGCGCATGAAGCGTACCGAGAGCGTGGTCGATGGTAGAAACCAGCCAACGTGCAGGGATATCCTGGGTCGGGCGCGGCAATg                                                                                                        <  2:263761/140‑1 (MQ=255)                                                     ttCAGCAAACGGGCAGTTGATATGGACTCCCCCCGCATGAAGCGTACCGAGAGCGTGGTCGATGGTAGAAACCAGCCAACGTGCAGGGATATCCTGGGTCGGGCGCGGCAATGAAATACTGTGCGTGGGGTGAGAGGCGa                                                                             >  1:686304/1‑140 (MQ=255)                                                       cAGCAAACGGGCGGTTGATACGGACTCCCCCCGCATGAATCGTACCGAGAGCGTGGTCGATGGTAGAAACCAGCCAACGTGCAGGGATATCATGGGTCGGGCGCGACAAGGAAAGACTGTGCGTGTGCTGAGAGGCGAAc                                                                           >  2:467895/1‑140 (MQ=255)                                                              cGGGCAGTTGATATGGACTCCCCCCGCATGAAGCGTACCGAGAGCGTGGTCGATGGTAGAAACCAGCCAACGTGCAGGGATATCCTGGGTCTGGCGCGGCCATGGAATGCTGTGCGTGGGGTGAGAGGCGAACATTCCCg                                                                    >  2:235983/1‑140 (MQ=255)                                                               gggCAGTTGATATGGACTCCCCCCGCATGAAGCGTACCGAGAGCGTGGTCGATGGTAGAAACCAGCCAACGTGCAGGGATATCCTGGGTCGGGCGCGGCAATGAAATACTGTGCGTGGGGTGAGAGGCGAACATTCCCgg                                                                   <  1:795778/140‑1 (MQ=255)                                                                      tGATATGGACTCCCCCCGCATGAAGCGTACCGAGAGCGTGGTCGATGGTAGAAACCAGCCAACGTGCAGGGATATCCTGGGTCGGGCGCGGCAATGAAATACTGTGCGTGGGGTGAGAGGCGAACATTCCCGGCTGGCGa                                                            <  2:205335/140‑1 (MQ=255)                                                                                  cccccGCATGAAGCGTACCGAGAGCGTGGTCGATGGTAGAAACCAGCCAACGTGCAGGGATATCCTGGGTCGGGCGCGGCAATGAAATACTGTGCGTGGGGTGAGAGGCGAACATTCCCGGCTGGCGAATTGCCTGATTc                                                >  1:577536/1‑140 (MQ=255)                                                                                  cccccGCATGAAGCGTACCGAGAGCGTGGTCGATGGTAGAAACCAGCCAACGTGCAGGGATATCCTGGGTCGGGCGCGGCAATGAAATACTGTGCGTGGGGTGAGAGGCGAACATTCCCGGCTGGCGAATTGCCTGATTc                                                >  1:24203/1‑140 (MQ=255)                                                                                  cccccGCATGAAGCGTACCGAGAGCGTGGTCGATGGTAGAAACCAGCCAACGTGCAGGGATATCCTGGGTCGGGCGCGGCAATGAAATACTGTGCGTGGGGTGAGAGGCGAACATTCCCGGCTGGCGAATTGCCTGATTc                                                >  1:15020/1‑140 (MQ=255)                                                                                  cccccGCATGAAGCGTACCGAGAGCGTGGTCGATGGTAGAAACCAGCCAACGTGCAGGGATATCCTGGGTCGGGCGCGGCAATGAAATACTGTGCGTGGGGTGAGA‑GCGAACATTCCCGGCTGGCGAATTGCCTGATTcg                                               >  2:111107/1‑140 (MQ=255)                                                                                         aTGAAGCGTACCGAGAGCGTGGTCGATGGTAGAAACCAGCCAACGTGCAGGGATATCATGGGTCGGGCGCGGCAATGAAATACTGTGCGTGGGGTGAGAGGCGAACATTCCCGGCTGGCGAATTGCCTGATTCGcgccgc                                         <  1:467895/140‑1 (MQ=255)                                                                                          tGAAGCGTACCGAGAGCGTGGTCGATGGTAGAAACCAGCCAACGTGCAGGGATATCCTGGGTCGGGCGCGGCAATGAAATACTGTGCGTGGGGTGAGAGGCGAACATTCCCGGCTGGCGAATTGCCTGATTCGCGCCGCa                                        <  2:686304/140‑1 (MQ=255)                                                                                                 tACCGAGAGCGTGGTCGATGGTAGAAACCAGCCAACGTGCAGGGATATCCTGGGTCGGGCGCGGCAATGAAATACTGTGCGTGGGGTGAGAGGCGAACATTCCCGGCTGGCGAATTGCCTGATTCGCGCCGCa                                        <  1:668614/133‑1 (MQ=255)                                                                                                 tACCGAGAGCGTGGTCGATGGTAGAAACCAGCCAACGTGCAGGGATATCCTGGGTCGGGCGCGGCAATGAAATACTGTGCGTGGGGTGAGAGGCGAACATTCCCGGCTGGCGAATTGCCTGATTCGCGCCGCa                                        >  2:668614/1‑133 (MQ=255)                                                                                                 tACCGAGAGCGTGGTCGATGGTAGAAACCAGCCAACGTGCAGGGATATCCTGGGTCGGGCGCGGCAATGAAATACTGTGCGTGGGGTGAGAGGCGAACATTCCCGGCTGGCGAATTGCCTGATTCGCGCCGCAGTCAAtt                                 >  2:749190/1‑140 (MQ=255)                                                                                                      agagCGTGGTCGATGGTAGAAACCAGCCAACGTGCAGGGATATCCTGGGTCGGGCGCGGCAATGAAATACTGTGCGTGGGGTGAGAGGCGAACATTCCCGGCTGGCGAATTGCCTGATTCGCGCCGCAGTCAATTAGCTc                            >  2:103994/1‑140 (MQ=255)                                                                                                            tGGTCGATGGTAGAAACCAGCCAACATGCAGGGATATCCTGGGGCGGGCGCGGCAATGAAATACTGTGCGTGGGGTGAGAGGCGAACATTCCCGGCTGGCGAATTGCCTGATTCGCGCCGCAGTCAATTAGCTCcggcgg                      >  2:686044/1‑140 (MQ=255)                                                                                                                  aTGGTAGAAACCAGCCAACGTGCAGGGATATCCTGGGTCGGGCGCGGCAATGAAATACTGTGCGTGGGGTGAGAGGCGAACATTCCCGGCTGGCGAATTGCCTGATTCGCGCCGCAGTCAATTAGCTCCGGCGGGCGATc                >  1:545766/1‑140 (MQ=255)                                                                                                                  aTGGTAGAAACCAGCCAACGTGCAGGGATATCCTGGGTCGGGCGCGGCAATGAAATACTGTGCGTGGGGTGAGA‑GCGAACATTCCCGGCTGGCGAATTGCCTGATTCGCGCCGCAGTCAATTAGCTCCGGCGGGCGATcg               <  1:111107/140‑1 (MQ=255)                                                                                                                   tGGTAGAAACCAGCCAACGTGCAGGGATATCCTGGGTCGGGCGCGGCAATGAAATACTGTGCGTGGGGTGAGAGGCGAACATTCCCGGCTGGCGAATTGCCTGATTCGCGCCGCAGTCAATTAGCTCCGGCGGGCGATcg               <  2:545766/140‑1 (MQ=255)                                                                                                                           aCCAGCCAACGTGCAGGGATATCCTGGGTCGGGCGCGGCAATGAAATACTGTGCGTGGGGTGAGAGGCGAACATTCCCGGCTGGCGAATTGCCTGATTCGCGCCGCAGTCAATTAGCTCCGGCGGGCGATCGGCGGTTaa       >  2:722495/1‑140 (MQ=255)                                                                                                                                ccAACGTGCAGGGATATCCTGGGTCGGGCGCGGCAATGAAATACTGTGCGTGGGGTGAGAGGCGAACATTCCCGGCTGGCGAATTGCCTGATTCGCGCCGCAGTCAATTAGCTCCGGCGGGCGATCGGCGGTTAAGAGaa  >  1:818583/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                   CCAGACGCTGTTGCCAGCTAAGCCCGGTATCGTCCATTTCGCCATACAGCGGTTCAGCAAACGGGCAGTTGATATGGACTCCCCCCGCATGAAGCGTACCGAGAGCGTGGTCGATGGTAGAAACCAGCCAACGTGCGGGGATATCCTGGGTCGGGCGCGGCAATGAAATACTGTGCGTGGGGTGAGAGGCGAACATTCCCGGCTGGCGAATTGCCTGATTCGCGCCGCAGTCAATTAGCTCCGGCGGGCGATCGGCGGTTAAGAGAA  >  NC_000913/2378692‑2378958

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑