Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,402,588 T→C T420A (ACC→GCC)  nuoC ← NADH:quinone oxidoreductase subunit CD

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,402,5880TC96.9% 107.0 / ‑7.0 32T420A (ACC→GCC) nuoCNADH:quinone oxidoreductase subunit CD
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (18/13);  minor base A (0/1);  total (18/14)
Fisher's exact test for biased strand distribution p-value = 4.38e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGCATTACGCGGGTGTAGCAGTCAGAAACGCCACCACCCACCGGGATTTCAAAGTCGAAGTTTTCATAGCCAGAATAAGGACGCGCCTTACGCACGTCGAAGTCGATCCCGGTAGCACGCAGGCCCGCGCCAGTGGTGCCCCACTCCAGCGCCTCTTTCGCGCCATAGGCGGCAACGCCCTGGGAACGACCTTTCAGAATGGTGTTTTGCAGCGCCGCTTTCTCGTAAGACGCCAGACGTTTCGGCATCCAGTCGAGGAACTCACGCAGCAG  >  NC_000913/2402452‑2402723
                                                                                                                                        |                                                                                                                                       
aGCATTACGCGGGTGTAGCAGTCAGAAACGCCACCACCCACCGGGATTTCAAAGTCGAAGTTTTCATAGCCAGAATAAGGACGCGCCTTACGCACGTCGAAGTCCATCGCGGTAGCATGCAGTCCCGCGCCTGTGGCGcc                                                                                                                                      >  2:416658/1‑140 (MQ=255)
     tACGCGGGTGTAGCACTAAGAATCGTCACTACCCACCCGGATTTCAAAGTCGAAGTTTTCATAGCCAGAATAAGGACGCGCCTTACGCACGTCGAAGTCGATCCCGGTAGCACGCAGGCCCGCGCCAGTGGCGCCCCACt                                                                                                                                 <  1:241535/140‑1 (MQ=255)
      aCGCGGGTGTAGCAGTCAGAAACGCCACCACCCACCGGGATTTCAAAGTCGAAGTTTTCATAGCCAGAATAAGGACGCGCCTTACGCACGTCGAAGTCGATCCCGGTAGCACGCAGGCCCGCGCCAGTGGCGCCCCACTc                                                                                                                                >  2:105662/1‑140 (MQ=255)
          gggTGTAGCAGTCAGAAACGCCACCACCCACCGGGATTTCAAAGTCGAAGTTTTCATAGCCAGAATAAGGACGCGCCTTACGCACGTCGAAGTCGATCCCGGTAGCACGCAGGCCCGCGCCAGTGGCGCCCCACTCCAgc                                                                                                                            >  2:635023/1‑140 (MQ=255)
                    gTCAGAAACGCCACCACCCACCGGGATTTCAAAGTCGAAGTTTTCATAGCCAGAATAAGGACGCGCCTTACGCACGTCGAAGTCGATCCCGGTAGCACGCAGGCCCGCGCCAGTGGCGCCCCACTCCAGCGCCTCTTTcg                                                                                                                  >  1:592724/1‑140 (MQ=255)
                      cAGAAACGCCACCGCCCACCGGGATTTCAAAGTCGAAGTTTTCATAGCCAGAATAAGGACGCGCCTTACGCACGTCGAAGTCGATCCCGGTAGCACGCAGGCCCCCGCCAGTGGCGCCCCACTCCAGCGCCTCTTTcgcg                                                                                                                >  1:826779/1‑140 (MQ=255)
                       aGAAACGCCACCACCCACCGGGATTTCAAAGTCGAAGTTTTCATAGCCAGAATAAGGACGCGCCTTACGCACGTCGAAGTCGATCCCGGTAGCGCGCAGGCCCGCGCCAGAGGCGCCACACTCCCGCGCCTCTTTCgcgc                                                                                                               >  1:327740/1‑140 (MQ=255)
                       aGAAACGCCACCACCCACCGGGATTTCAAAGTCGAAGTTTTCATAGCCAGAATAAGGACGCGCCTTACGCACGTCGAAGTCGATCCCGGTAGCACGCAGGCCCGCGCCAGTGGCGCCCCACTcc                                                                                                                               >  2:558533/1‑124 (MQ=255)
                       aGAAACGCCACCACCCACCGGGATTTCAAAGTCGAAGTTTTCATAGCCAGAATAAGGACGCGCCTTACGCACGTCGAAGTCGATCCCGGTAGCACGCAGGCCCGCGCCAGTGGCGCCCCACTcc                                                                                                                               <  1:558533/124‑1 (MQ=255)
                         aaaCGCCACCACCCACCGGGATTTCAAAGTCGAAGTTTTCATAGCCAGAATAAGGACGCGCCTTACGCACGTCGAAGTCGATCCCGGTAGCACGCAGGCCCGCGCCAGTGGCGCCCCACTCCAGCGCCTCTTTCGCGCCa                                                                                                             >  1:743869/1‑140 (MQ=255)
                                   acccaccGGGATTTCAAAGTCGAAGTTTTCATAGCCAGAATAAGGACGCGCCTTACGCACGTCGAAGTCGATCCCGGTAGCACGCAGGCCCGCGCCAGTGGCGCCCCACTCCAGCGCCTCTTTCGCGCCATAGGCGGCaa                                                                                                   <  2:90301/140‑1 (MQ=255)
                                   acccaccGGGATTTCAAAGTCGAAGTTTTCATAGCCAGAATAAGGACGCGCCTTACGCACGTCGAAGTCGATCCCGGTAGCACGCAGGCCCGCGCCAGTGGCGCCCCACTCCAGCGCCTCTTTCGCGCCATAGGCGGCaa                                                                                                   <  1:105662/140‑1 (MQ=255)
                                   acccaccGGGATTTCAAAGTCGAAGTTTTCATAGCCAGAATAAGGACGCGCCTTACGCACGTCGAAGTCGATCCCGGTAGCACGCAGGCCCGCGCCAGTGGCGCCCCACTCCAGCGCCTCTTTCGCGCCATAGGCGGCaa                                                                                                   <  1:635023/140‑1 (MQ=255)
                                               ttCAAAGTCGAAGTTTTCATAGCCAGAATAAGGACGCGCCTTACGCACGTCGAAGTCGATCCCGGTAGCACGCAGGCCCGCGCCAGTGGCGCCCCACTCCAGCGCCTCTTTCGCGCCATAGGCGGCAACGCCCTGGGAAc                                                                                       >  1:515104/1‑140 (MQ=255)
                                                tCAAAGTCGAAGTTTTCATAGCCAGAATAAGGACGCGCCTTACACACGTCGAAGTCGATCCCGGTAGCACGCAGGCCCGCGCCAGTGGCGCCCCACTCCAGCGCCTCTTTCGCGCCATAGGCGGCAACGCCCTGGGAACg                                                                                      <  1:164550/140‑1 (MQ=255)
                                                   aaGTCGAAGTTTTCATACCCAGTATAAGGACCCGCCTTACGCACGTCGTAGTCGATCCCGGTAGCGCGCAGGCCCGCGCCAGTGGCGCCCCACTCCAGCGCCTCTTTCGCGCCATAGGCGGCAACGCCCTGGGAACGGcc                                                                                   <  2:327740/140‑1 (MQ=255)
                                                       cGAAGTTTTCATAGCCAGAATAAGGACGCGCCTTACGCACGTCGAAGTCGATCCCGGTAGCACGCAGGCCCGCGCCAGTGGCGCCCCACTCCAGCGCCTCTTTCGCGCCATAGGCGGCAACGCCCTggg                                                                                          <  1:559101/129‑1 (MQ=255)
                                                       cGAAGTTTTCATAGCCAGAATAAGGACGCGCCTTACGCACGTCGAAGTCGATCCCGGTAGCACGCAGGCCCGCGCCAGTGGCGCCCCACTCCAGCGCCTCTTTCGCGCCATAGGCGGCAACGCCCTggg                                                                                          >  2:559101/1‑129 (MQ=255)
                                                           gTTTTCATAGCCAGAATAAGGACGCGCCTTACGCACGTCGAAGTCGATCCCGGTAGCACGCAGGCCCGCGCCAGTGGCGCCCCACTCCAGCGCCTCTTTCGCGCCATAGGCGGCAACGCCCTGGGAACGACCTTTCAGaa                                                                           <  2:743869/140‑1 (MQ=255)
                                                                     ccAGAATAAGGACGCGCCTTACGCACGTCGAAGTCGATCCCGGTAGTACGCAGGCCCGCGCCAGTGGCGCCCCACTCCAGCGCCTCTTTCGCGCCATAGGCGGCAACGCCCTGGGAACGACCTTTCAGAATGGTGTTTTg                                                                 >  1:162933/1‑140 (MQ=255)
                                                                      cAGAATAAGGACGCGCCTTACGCACGTCGAAGTCGATCCCGGTAGCACGCAGGCCCGCGCCAGTGGCGCCCCACCCCAGCGCCTCTTTCGCGCCATAGGCGGCAACGCCCTGGGAACGACCTTTCAGAATGGCGTTTTGc                                                                >  2:835344/1‑140 (MQ=255)
                                                                      cAGAATAAGGACGCGCCTTACGCACGTCGAAGTCGATCCCGGTAGCACGCAGGCCCGCGCCAGTAGCGCCCCACTCCAGCGCCTCTTTCTCGCCATAGGCGGCAACGCCCTGGGAACGACCTTTCAGAATGGTGtttttc                                                                >  2:197847/1‑138 (MQ=255)
                                                                        gAATAAGGACGCGCCTTACGCACGTCGAAGTCGATCCCGGTAGCACGCAGGCCCGCGCCAGTGGCGCCCCACTCCAGCGCCTCTTTCGCGCCATAGGCGGCAACGCCCTGGGAACGACCTTTCAGAATGGTGTTTTGCAg                                                              <  2:515104/140‑1 (MQ=255)
                                                                          aTAAGGACGCGCCTTACGCACGTCGAAGTCGATCCCGGTAGCACGCAGGCCCGCGCCAGTGGCGCCCCACTCCAGCGCCTCTTTCGCGCCATAGGCGGCAACGCCCTGGGAACGACCTTTCAGAATGGTGTTTTGCAgcg                                                            <  2:641064/140‑1 (MQ=255)
                                                                                   cgcCTTACGCACGTCGAAGTCGATCCCGGTAGCACGCAGGCCCGCGCCAGTGGCGCCCCACTCCAGCGCCTCTTTCGCGCCATAGGCGGCAACGCCCTGGGAACGACCTTGCAGAATCGTGTTTCGCAGCGCCGCTTtct                                                   >  1:416215/1‑140 (MQ=255)
                                                                                   cgcCTTACGCACGTCGAAGTCGATCCCGGTAGCACGCAGGCCCGCGCCAGTGGCGCCCCACTCCAGCGCCTCTTTCGCGCCATAGACGGCAACGCCCTGGGAACGACCTTTCAGAATGGTGTTTTGCAGCGCCGCTTtct                                                   >  2:124188/1‑140 (MQ=255)
                                                                                               gTCGAAGTCGATCCCGGTAGCACGCAGGCCCGCGCCAGTGGCGCCCCACTCCAGCGCCTCTTTCGCGCCATAGGCGGCAACGCCCTGGGAACGACCTTTCAGAATGGTGTTTTGCAGCGCCGCTTTCTCGTAAGACGCCa                                       >  1:595293/1‑140 (MQ=255)
                                                                                                         aTCCCGGTAGCACGCAGGCCCGCGCCAGTGGCGCCCCACTCCAGCGCCTCTTTCGCGCCATAGGCGGCAACGCCCTGGGAACGACCTTTCAGAATGGTGTTTTGCAGCGCCGCTTTCTCGTAAGACGCCAGACGTTTCgg                             >  1:641068/1‑140 (MQ=255)
                                                                                                          tCCCGGTAGCACGCAGTCCCGCGCCAGTGGAACCCGACTACAGCGCCTCTTTCGCGCCATAGGCGGCAACGCCCTGGGAACGACCTTTCAGAATGGTGTTTTGCAGCGCCGCTTTCTCGTAAGACGCCAGACGTTTCGGc                            <  2:416215/140‑1 (MQ=255)
                                                                                                                      gCAGGCCCGCGCCAGTGGCGCCCCACTCCAGCGCCTCTTTCGCGCCATAGGCGGCAACGCCCTGGGAACGACCTTTCAGAATGGTGTTTTGCAGCGCCGCTTTCTCGTAAGACGCCAGACGTTTCGGCATCCAGTCGAgg                >  2:549433/1‑140 (MQ=255)
                                                                                                                      gCAGGCCCGCGCCAGTGGCGCCCCACTCCAGCGCCTCTTTCGCGCCATAGGCGGCAACGCCCTGGGAACGACCTTTCAGAATGGTGTTTTGCAGCGCCGCTTTCTCGTAAGACGCCAGACGTTTCGGCATCCAGTCGAgg                <  2:464496/140‑1 (MQ=255)
                                                                                                                      gCAGGCCCGCGCCAGTGGCGCCCCACTCCAGCGCCTCTTTCGCGCCATAGGCGGCAACGCCCTGGGAACGACCTTTCAGAATGGTGTTTTGCAGCGCCGCTTTCTCGTAAGACGCCAGACGTTTCGGCATCCAGTCGAgg                >  1:464496/1‑140 (MQ=255)
                                                                                                                      gCAGGCCCGCGCCAGTGGCGCCCCACTCCAGCGCCTCTTTCGCGCCATAGGCGGCAACGCCCTGGGAACGACCTTTCAGAATGGTGTTTTGCAGCGCCGCTTTCTCGTAAGACGCCAGACGTTTCGGCATCCAGTCGAgg                <  1:124188/140‑1 (MQ=255)
                                                                                                                        aGGCCCGCGCCAGTGGCGCCCCACTCCAGCGCCTCTTTCGCGCCATAGGCGGCAACGCCCTGGGAACGACCTTTCAGAATGGTGTTTTGCAGCGCCGCTTTCTCGTAAGACGCCAGACGTTTCGGCATCCAGTCGAGGaa              >  2:717200/1‑140 (MQ=255)
                                                                                                                                    gtggcgCCCCACTCCAGCGCCTCTTTCGCGCCATAGGCGGCAACGCCCTGGGAACGACCTTTCAGAATGGTGTTTTGCAGCGCCGCTTTCTCGTAAGACGCCAGACGTTTCGGCATCCAGTCGAGGAACTCACGcagcag  <  2:641068/140‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                       
AGCATTACGCGGGTGTAGCAGTCAGAAACGCCACCACCCACCGGGATTTCAAAGTCGAAGTTTTCATAGCCAGAATAAGGACGCGCCTTACGCACGTCGAAGTCGATCCCGGTAGCACGCAGGCCCGCGCCAGTGGTGCCCCACTCCAGCGCCTCTTTCGCGCCATAGGCGGCAACGCCCTGGGAACGACCTTTCAGAATGGTGTTTTGCAGCGCCGCTTTCTCGTAAGACGCCAGACGTTTCGGCATCCAGTCGAGGAACTCACGCAGCAG  >  NC_000913/2402452‑2402723

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑