Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,415,078	T→C	V111A (GTC→GCC)	pta →	phosphate acetyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,415,078	0	T	C	100.0%	104.3 / NA	30	V111A (GTC→GCC)	pta	phosphate acetyltransferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (11/19);  total (11/19)

TGAACCGCTGAAAATGAGCTACGTTGAAGGTCTGCTTTCCAGCAATCAGAAAGATGTGCTGATGGAAGAGATCGTCGCAAACTACCACGCTAACACCAAAGACGCTGAAGTCGTTCTGGTTGAAGGTCTGGTCCCGACACGTAAGCACCAGTTTGCCCAGTCTCTGAACTACGAAATCGCTAAAACGCTGAATGCGGAAATCGTCTTCGTTATGTCTCAGGGCACTGACACCCCGGAACAGCTGAAAGAGCGTATCGAACTGA  >  NC_000913/2414947‑2415209                                                                                                                                    |                                                                                                                                    tgaaccgctgaaAATGAGCTACGTTGAAGGTCTGCTTTCCAGCAATCAGAAAGATGTGCTGATGGAAGAGATCGTCGCAAACTACCACGCTAACACCAAAGACGCTGAAGTCGTTCTGGTTGAAGGTCTGGCCCCGacac                                                                                                                             <  1:105013/140‑1 (MQ=255)        ctgaaAATGAGCTACGTTGAAGGTCTGCTTTCCAGCAATCAGAAAGATGTGCTGATGGAAGAGATCGTCGCAGACTACCACGCTAACACCAAAGACGCTGAAGTCGTTCTGGTTGAAGGTCTGGCCCCGACACGTAAGCa                                                                                                                      <  1:628379/140‑1 (MQ=255)        ctgaaAATGAGCTACGTTGAAGGTCTGCTTTCCAGCAATCAGAAAGATGTGCTGATGGAAGAGATCGTCGCAAACTACCACGCTAACACCAAAGACGCTGAAGTCGTTCTGGTTGAAGGTCTGGCCCCGACACGTAAGCa                                                                                                                      <  2:468481/140‑1 (MQ=255)                   cTACGTTGAAGGTCTGCTTTCCGGCCATCAGAAAGATGTGCTGATGGAAGAGATCGTCGCAAACTACCACGCTAACACCAAAGACGCTGAAGTCGTTCTGGTTGAAGGTCTGGCCCCGACACGTAAGCACCAGTTTGccc                                                                                                           <  1:397692/140‑1 (MQ=255)                   cTACGTTGAAGGTCTGCTTTCCAGCAATCAGAAAGATGTGCTGATGGAAGAGATCGTCGCAAACTACCACGCTAACACCAAAGACGCTGAAGTCGTTCTGGTTGAAGGTCTGGCCCCGACACGTAAGCACCAGTTTGccc                                                                                                           <  2:62050/140‑1 (MQ=255)                                 tGCTTTCCAGCAATCAGAAAGATGTGCTGATGGAAGAGATCGTCGCAAACTACCACGCTAACACCAAAGACGCTGAAGTCGTTCTGGTTGAAGGTCTGGCCCCGACACGTAAGCACCAGTTTGCCCAGTCTCTGAACTAc                                                                                             >  1:469533/1‑140 (MQ=255)                                      tCCAGCAATCAGAAAGATGTGCTGATGGAAGAGATCGTCGCAAACTACCACGCTAACACCAAAGACGCTGAAGTCGTTCTGGTTGAAGGTCTGGCCCCGACACGTAAGCACCAGTTTGCCCAGTCTCTGAACTACGAAAt                                                                                        <  1:456299/140‑1 (MQ=255)                                             aTCAGAAAGATGTGCTGATGGAAGAGATCGTCGCAAACTACCACGCTAACACCAAAGACGCTGAAGTCGTTCTGGTTGAAGGTCTGGCCCCGACACGTAAGCACCAGTTTGCCCAGTCTCTGAACTACGAAATCGCTaaa                                                                                 >  1:148754/1‑140 (MQ=255)                                                  aaaGATGTGCTGATGGAAGAGATCGTCGCAACCTATCACCCTAACACCAAAGACGCTGAAGTCGTTCTGGTTGAAGGTCTGGCCCCGACACGTAAGCACCAGTTTGCCCAGTCTCTGAACTACGAAATCGCTAAAACGCt                                                                            <  2:272288/140‑1 (MQ=255)                                                    aGATGTGCTGATGGAAGAGATCGTCGCAAACTACCACGCTAACACCAAAGACGCTGAAGTCGTTCTGGTTGAAGGTCTGGCCCCGACACGTAAGCACCAGTTTGCCCAGTCTCTGAACTACGAAATCGCTAAAACGCTGa                                                                          <  2:469533/140‑1 (MQ=255)                                                               tGGAAGAGATCGTCGCAAACTACCACGCTAACACCAAAGACGCTGAAGTCGTTCTGGTTGAAGGTCTGGCCCCGACACGTAAGCACCAGTTTGCCCAGTCTCTGAACTACGAAATCGCTAAAGCGCTGAATGCGGAAATc                                                               >  2:746708/1‑140 (MQ=255)                                                                        tcgtcgCAAACTACCACGCTAACACCAAAGACGCTGAAGTCGTTCTGGTTGAAGGTCTGGCCCCGACACGTAAGCACCAGTTTGCCCAGTCTCTGAACTACGAAATCGCTAAAACGCTGAATGCGGAAATCGTCTTCGtt                                                      <  1:602458/140‑1 (MQ=255)                                                                        tcgtcgCAAACTACCACGCTAACACCAAAGACGCTGAAGTCGTTCTGGTTGAAGGTCTGGCCCCGACACGTAAGCACCAGTTTGCCCAGTCTCTGAACTACGAAATCGCTAAAACGCTGAATGCGGAAATCGTCTTCGtt                                                      >  2:602458/1‑140 (MQ=255)                                                                          gtcgCAAACTACCACGCTAACACCAAAGACGCTGAAGTCGTTCTGGTTGAAGGTCTGGCCCCGACACGTAAGCACCAGTTTGCCCAGTCTCTGAACTACGAAATCGCTAAAACGCTGAATGCGGAAATCGTCTTCGTTAt                                                    >  2:197982/1‑140 (MQ=255)                                                                            cgCAAACTACCACGCTAACACCAAAGACGCTGAAGTCGTTCTGGTTGAAGGTCTGGCCCCGACACGTAAGCACCAGTTTGCCCAGTCTCTGAACTACGAAATCGCTAAAACGCTGAATGCGGAAATCGt                                                             >  1:601741/1‑129 (MQ=255)                                                                            cgCAAACTACCACGCTAACACCAAAGACGCTGAAGTCGTTCTGGTTGAAGGTCTGGCCCCGACACGTAAGCACCAGTTTGCCCAGTCTCTGAACTACGAAATCGCTAAAACGCTGAATGCGGAAATCGt                                                             <  2:601741/129‑1 (MQ=255)                                                                              cAAACTACCACGCTAACACCAAAGACGCTGAAGTCGTTCTGGTTGAAGGTCTGGCCCCGACACGTAAGCACCAGTTTGCCCAGTCTCTGAACTACGAAATCGCTAAAACGCTGAATGCGGAAATCGTCTTCGTTATGtct                                                <  1:746708/140‑1 (MQ=255)                                                                                     ccACGCTAACACCAAAGACGCTGAAGTCGTTCTGGTTGAAGGTCTGGCCCCGACACGTAAGCACCAGTTTGCCCAGTCTCTGAACTACGAAATCGCTAAAACGCTGAATGCGGAAATCGTCTTCGTTATGTCTCAGGGCa                                         >  1:465680/1‑140 (MQ=255)                                                                                         gCTAACACCAAAGACGCTGAAGTCGTTCTGGTTGAAGGTCTGGCCCCGACACGTAAGCACCAGTTTGCCCAGTCTCTGAACTACGAAATCGCTAAAACGCTGAATGCGGAAATCGTCTTCGTTATGTCTCAGGGCACTGa                                     <  2:148754/140‑1 (MQ=255)                                                                                                  aaaGACGCTGAAGTCGTTCTGGTTGAAGGTCTGGCCCCGACACGTAAGCACCAGTTTGCCCAGTCTCTGAACTACGAAATCGCTAAAACGCTGAATGCGGAAATCGTCTTCGTTATGTCTCAGGGCACTGACACCCCGGa                            <  2:465680/140‑1 (MQ=255)                                                                                                   aaGACGCTGAAGTCGTTCTGGTTGAAGGTCTGGCCCCGACACGTAAGCACCAGTTTGCCCAGTCTCTGAACTACGAAATCGCTAAAACGCTGAATGCGGAAATCGTCTTCGTTATGTCTCAGGGCACTGACACCCCGGaa                           <  1:708364/140‑1 (MQ=255)                                                                                                   aaGACGCTGAAGTCGTTCTGGTTGAAGGTCTGGCCCCGACACGTAAGCACCAGTTTGCCCAGTCTCTGAACTACGAAATCGCTAAAACGCTGAATGCGGAAATCGTCTTCGTTATGTCTCAGGGCACTGACACCCCGGaa                           >  2:628410/1‑140 (MQ=255)                                                                                                       cGCTGAAGTCGTTCTGGTTGAAGGTCTGGCCCCGACACGTAAGCACCAGTTTGCCCAGTCTCTGAACTACGAAATCGCTAAAACGCTGAATGCGGAAATCGTCTTCGTTATGTCTCAGGGCACTGACACCCCGGaa                           <  1:490624/136‑1 (MQ=255)                                                                                                       cGCTGAAGTCGTTCTGGTTGAAGGTCTGGCCCCGACACGTAAGCACCAGTTTGCCCAGTCTCTGAACTACGAAATCGCTAAAACGCTGAATGCGGAAATCGTCTTCGTTATGTCTCAGGGCACTGACACCCCGGaa                           >  2:490624/1‑136 (MQ=255)                                                                                                         cTGAAGTCGTTCTGGTTGAAGGTCTGGCCCCGACACGTAAGCACCAGTTTGCCCAGTCTCTGAACTACGAAATCGCTAAAACGCTGAATGCGGAAATCGTCTTCGTTATGTCTCAGGGCACTGACACCCCGGaa                           >  1:197472/1‑134 (MQ=255)                                                                                                         cTGAAGTCGTTCTGGTTGAAGGTCTGGCCCCGACACGTAAGCACCAGTTTGCCCAGTCTCTGAACTACGAAATCGCTAAAACGCTGAATGCGGAAATCGTCTTCGTTATGTCTCAGGGCACTGACACCCCGGaa                           <  2:676267/134‑1 (MQ=255)                                                                                                         cTGAAGTCGTTCTGGTTGAAGGTCTGGCCCCGACACGTAAGCACCAGTTTGCCCAGTCTCTGAACTACGAAATCGCTAAAACGCTGAATGCGGAAATCGTCTTCGTTATGTCTCAGGGCACTGACACCCCGGaa                           <  2:197472/134‑1 (MQ=255)                                                                                                         cTGAAGTCGTTCTGGTTGAAGGTCTGGCCCCGACACGTAAGCACCAGTTTGCCCAGTCTCTGAACTACGAAATCGCTAAAACGCTGAATGCGGAAATCGTCTTCGTTATGTCTCAGGGCACTGACACCCCGGaa                           >  1:676267/1‑134 (MQ=255)                                                                                                                         tGAAGGTCTGGCCCCGACACGTAAGCACCAGTTTGCCCAGTCTCTGAACTACGAAATCGCTAAAACGCTGAATGCGGAAATCGTCTTCGTTATGTCTCAGGGCACTGACACCCCGGAACAGCTGAAAGAGCGTATCGAAc     <  1:73319/140‑1 (MQ=255)                                                                                                                            aGGTCTGGCCCCGACACGTAAGCACCAGTTTGCCCAGTCTCTGAACTACGAAATCGCTAAAACGCTGAATGCGGAAATCGTCTTCGTTATGTCTCAGGGCACTGACACCCCGGAACAGCTGAAAGAGCGTATCGAACTGa  <  1:628410/140‑1 (MQ=255)                                                                                                                                    |                                                                                                                                    TGAACCGCTGAAAATGAGCTACGTTGAAGGTCTGCTTTCCAGCAATCAGAAAGATGTGCTGATGGAAGAGATCGTCGCAAACTACCACGCTAACACCAAAGACGCTGAAGTCGTTCTGGTTGAAGGTCTGGTCCCGACACGTAAGCACCAGTTTGCCCAGTCTCTGAACTACGAAATCGCTAAAACGCTGAATGCGGAAATCGTCTTCGTTATGTCTCAGGGCACTGACACCCCGGAACAGCTGAAAGAGCGTATCGAACTGA  >  NC_000913/2414947‑2415209

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑