Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,432,681	G→A	R88C (CGT→TGT)	folC ←	bifunctional folylpolyglutamate synthetase/dihydrofolate synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,432,681	0	G	A	100.0%	64.3 / NA	21	R88C (CGT→TGT)	folC	bifunctional folylpolyglutamate synthetase/dihydrofolate synthetase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (11/10);  total (11/10)

ACAACGCCGACAGCGTACCGTACTCGAAATAGGTCAGGGAAATATCACCGCGTGCCGATTCAATCTCCGCAAAAGAGGCGGTGTGGGCCGATTCCGGCAATTCCTGGCCCTGCACACGTACGCGCTCGGTATAACGCACCAGATGAGGCGAACTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTT  >  NC_000913/2432546‑2432807                                                                                                                                        |                                                                                                                               acaACGCCGACAGCGTACCGTACTCGAAATAGGTCAGGGAAATATCACCGCGTGCCGATTCAATCTCCGCAAAAGAGGCGGTGTGGGCCGATTCCGGCAATTCCTGGCCCTGCACACGGACGCCCTCGGGTTAACACAcc                                                                                                                            >  2:36988/1‑140 (MQ=255)   aaCGCCGACAGCGTACCATACTTGAAATAGGGCCGCGAAGTATCACCGCGTGCCGATTCAATCTCCGCAAAAGAGGCGGTGTGGGCCGATTCCGGCAATTCCTGGCCCTGCACACGTACGCGCTCGGTATAACACACCAg                                                                                                                          <  2:399092/140‑1 (MQ=255)             gCGTACCGTACTCGAAATAGGTCAGGGAAATATCACCGCGTGCCGATTCAATCTCCGCAAAAGAGGCGGTGTGGGCCGATTCCGGCAATTCCTGGCCCTGCACACGTACGCGCTCGGTATAACACACCAGATGAGGCGaa                                                                                                                >  2:48765/1‑140 (MQ=255)                taccgtacTCGAAATAGGTCAGGGAAATATCACCGCGTGCCGATTCAATCTCCGCAAAAGAGGCGGTGTGGGCCGATTCCGGCAATTCCTGGCCCTGCACACAGACGCGCACGGTATAACACACCAGATGAGGCGAACTg                                                                                                             >  1:329355/1‑140 (MQ=255)                  ccgtacTCGAAATAGGTCAGGGAAATATCACCGCGTGCCGATTCAATCTCCGCAAAAGAGGCGGTGTGGGCCGATTCCGGCAATTCCTGGCCCTGCACACGTACGCGCTCGGTATAACACACCAGATGAGGCGAACTGTa                                                                                                           <  1:36988/140‑1 (MQ=255)                       cTCGAAATAGGTCAGGGAAATATCACCGCGTGCCGATTCAATCTCCGCAAAAGAGGCGGTGTGGGCCGATTCCGGCAATTCCTGGCCCTGCACACGTACGCGCTCGGTATAACACACCAGATGAGGCGAACTGTAGACGc                                                                                                      <  1:656923/140‑1 (MQ=255)                                 gTCAGGGAAATATCACCGCGTGCCGATTCAATCTCCGCAAAAGAGGCGGTGTGGGCCGATTCCGGCAATTCCTGGCCCTGCACACGTACGCGCTCGGTATAACACACCAGATGAGGCGAACTGTAGACGCCCACTTTGTa                                                                                            >  1:77914/1‑140 (MQ=255)                                               aCCGCGTGCCGATTCAATCTCCGCAAAAGAGGCGGTGTGGGCCGATTCCGGCAATTCCTGGCCCTGCACACGTACGCGCTCGGTATAACACACCAGATGAGGCGAACTGTAGACGCCCACTTTGTACCCTGCCGCCATCa                                                                              <  1:48765/140‑1 (MQ=255)                                                ccGCGTGCCGATTCAATCTCCGCAAAAGAGGCGGTGTGGGCCGATTCCGGCAATTCCTGGCCCTGCACACGTACGCGCTCGGTATAACACACCAGATGAGGCGAACTGTAGACGCCCACTTTGTACCCt                                                                                        <  2:437312/129‑1 (MQ=255)                                                ccGCGTGCCGATTCAATCTCCGCAAAAGAGGCGGTGTGGGCCGATTCCGGCAATTCCTGGCCCTGCACACGTACGCGCTCGGTATAACACACCAGATGAGGCGAACTGTAGACGCCCACTTTGTACCCt                                                                                        >  1:437312/1‑129 (MQ=255)                                                ccGCGTGCCGATTCAATCTCCGCAAAAGAGGCGGTGTGGGCCGATTCCGGCAATTCCTGGCCCTGCACACGTACGCGCTCGGTATAACACACCAGATGAGGCGAACTGTAGACGCCCACTTTGTACCCTGCCGCCATCAg                                                                             >  1:489854/1‑140 (MQ=255)                                                    gTGCCGATTCAATCTCCGCAAAAGAGGCGGTGTGGGCCGATTCCGGCAATTCCTGGCCCTGCACACGTACGCGCTCGGTATAACACACCAGATGAGGCGAACTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATc                                                                         >  1:560766/1‑140 (MQ=255)                                                         gATTCAATCTCCGCAAAAGAGGCGGTGTGGGCCGATTCCGGCAATTCCTGGCCCTGCACACGTACGCGCTCGGTATAACACACCAGATGAGGCGAACTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTc                                                                    <  2:458369/140‑1 (MQ=255)                                                                          agagGCGGTGTGGGCCGCTTCCTGCAATTCCCGGCGCTGCACACGTACTCGCTCGGTATAACACACCAGATGAGGCGAACTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAggtgg                                                   <  2:329355/140‑1 (MQ=255)                                                                            agGCGGTGTGGGCCGATTCCGGCAATTCCTGGCCCTGCACACGTACGCGCTCGGTATAACACACCAGATGAGGCGAACTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGgtggtg                                                 <  2:560766/140‑1 (MQ=255)                                                                                                 gCAATTCCTGGCCCTGCACACGTACGCGCTCGGTATAACACACCAGATGAGGCGAACTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGTGGTGCCTTTGCCATTCGTACCCGCa                            <  2:489854/140‑1 (MQ=255)                                                                                                               tGCACACGTACGCGCTCGGTATAACACACCAGATGAGGCGAACTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGTGTTGCCTTTGCCAATCGTACCCGAAACGGTAAACACaaa              >  1:330914/1‑140 (MQ=255)                                                                                                                gCACACGTACGCGCTCGGTATAACACACCAGATGAGGCGAACTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAAt             >  1:811959/1‑140 (MQ=255)                                                                                                                 cacaCGTACGCGCTCGGTATAACACACCAGATGAGGCGAACTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATg            >  1:628232/1‑140 (MQ=255)                                                                                                                    ccGTACGCGCTCGGTATAACACACCAGATGAGGCGAACTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTATGGCAGGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGgcg         <  2:77914/139‑1 (MQ=255)                                                                                                                     cGTACGCGCTCGGTATAACACACCAGATGAGGCGAACTGTATACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGGGGTGCCTTTCCCATTCGTACCCGCAACGGTAAACACAAATGgcga        >  2:122240/1‑139 (MQ=255)                                                                                                                      gTACGCGCTCGGTATAACACACCAGATGAGGCGAACTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCt       >  2:849716/1‑140 (MQ=255)                                                                                                                           cgcTCGGTATAACACACCAGATGAGGCGAACTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGTGGTGCCTTTGCCATTCGTACCCGCATCGATAAACATAAATGGCGCTGGttt  >  1:399853/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                               ACAACGCCGACAGCGTACCGTACTCGAAATAGGTCAGGGAAATATCACCGCGTGCCGATTCAATCTCCGCAAAAGAGGCGGTGTGGGCCGATTCCGGCAATTCCTGGCCCTGCACACGTACGCGCTCGGTATAACGCACCAGATGAGGCGAACTGTAGACGCCCACTTTGTACCCTGCCGCCATCAGAATCGACTCCAGCGTACGGCAGGTGGTGCCTTTGCCATTCGTACCCGCAACGGTAAACACAAATGGCGCTGGTTT  >  NC_000913/2432546‑2432807

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑