Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,456,619	T→C	D65G (GAT→GGT)	sixA ←	phosphohistidine phosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,456,619	0	T	C	100.0%	167.1 / NA	48	D65G (GAT→GGT)	sixA	phosphohistidine phosphatase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (22/26);  total (22/26)

GTAAGTGAGAGATCACCAGTACCGAAGCAACACCTTCATTGGTCAACGCCTGCAAATAGGCACTGACCAGACCAACATCGCCGCAGGGCGTTAACTCCGGCAGAACTTCTGCACTGGAAGGCAGGTTCAGACAATCCCCTACTTCTTCCAGTGTTTGCTCGGCTCGCAGGAACGGGCTCACCAGAACGCGTTCGATTTCCACTTTTTGACCTTTCAGCCAGTTCGCCATCAGGCGAGATTCGTCACAACCATTAGTGGTCAGAGGACGAA  >  NC_000913/2456485‑2456754                                                                                                                                       |                                                                                                                                        gTAAGTGAGAGATCACCAGTACCGAAGCAACACCTTCATTGGTCAACGCCTGCAAATAGGCACTGACCAGACCAACATCGCCGCAGGGCGTTAACTCCGGCAGAACTTCTGCACTGGAAGGCAGGTTCAGACAACCCCCt                                                                                                                                    <  2:565976/140‑1 (MQ=255) gTAAGTGAGAGATCACCAGTACCGAAGCAACACCTTCATTGGTCAACGCCTGCAAATAGGCACTGACCAGACCAACATCGCCGCAGGGCGTGAACTCCGGTAGAGCTTTTGCACTGGAAGGCAGGTTCAGACAACCCCCt                                                                                                                                    >  2:351314/1‑140 (MQ=255)    aGTGAGAGATCACCAGTACCGACGCCACACCTTCATTGGTCAACGCCTGCAAATAGGCACTGACCAGACCAACATCGCCGCAGGGCGTTAACTCCGGCAGAACTTCTGCACTGGAAGGCAGGTTCAGACAACCCCCTAct                                                                                                                                 <  1:351314/140‑1 (MQ=255)    aGTGAGAGATCACCAGTACCGAAGCAACACCTTCATTGGTCAACGCCTGCAAATAGGCACTGACCAGACCAACATCGCCGCAGGGCGTTAACTCCGGCAGAACTTCTGCACTGGAAGGCAGGTTCAGACAACCCCCTAct                                                                                                                                 <  1:77723/140‑1 (MQ=255)    aGTGAGAGATCACCAATACCGACGCTACACCTTCATTTGTCAACGCCTGCAAATAGGCACTGACCAGACCAACATCGCCGCAGGGCGTTAACTCCGGCAGAACTTCTGCACTGGAAGGCAGGTTCAGACAACCCCCTAct                                                                                                                                 <  1:242739/140‑1 (MQ=255)          agaTCACCAGTACCGAAGCAACACCTTCATTGGTCAACGCCTGCAAATAGGCACTGACCAGACCAACATCGCCGCAGGGCGTTAACTCCGGCAGAACTTCTGCACTGGAAGGCAGGTTCAGACAACCCCCTACTTCTTcc                                                                                                                           <  1:207438/140‑1 (MQ=255)                         aaGCAACACCTTCATTGGTCAACGCCTGCAAATAGGCACTGACCAGACCAACATCGCCGCAGGGCGTTAACTCCGGCAGAACTTCTGCACTGGAAGGCAGGTTCAGACAACCCCCTACTTCTTCCAGTGTTTgctcggct                                                                                                            >  1:69667/1‑140 (MQ=255)                         aaGCAACACCTTCATTGGTCAACGCCTGCAAATAGGCACTGACCAGACCAACATCGCCGCAGGGCGTTAACTCCGGCAGAACTTCTGCACTGGAAGGCAGGTTCAGACAACCCCCTACTTCTTCCAGTGTTTgctcggct                                                                                                            >  2:493225/1‑140 (MQ=255)                              acacCTTCATTGGTCAACGCCTGCAAATAGGCACTGACCAGACCAACATCGCCGCAGGGCGTTAACTCCGGCAGAACTTCTGCACTGGAAGGCAGGTTCAGACAACCCCCTACTTCTTCCAGTGTTTgctcggctcg                                                                                                          >  1:629839/1‑137 (MQ=255)                              acacCTTCATTGGTCAACGCCTGCAAATAGGCACTGACCAGACCAACATCGCCGCAGGGCGTTAACTCCGGCAGAACTTCTGCACTGGAAGGCAGGTTCAGACAACCCCCTACTTCTTCCAGTGTTTgctcggctcg                                                                                                          <  2:629839/137‑1 (MQ=255)                              acacCTTCATTGGTCAACGCCTGCAAATAGGCACTGACCAGACCAACATCGCCGCAGGGCGTTAACTCCGGCAGAACTTCTGCACTGGAAGGCAGGTTCAGACAACCCCCTACTTCTTCCAGTGTTTGCTCGGCTCGCAg                                                                                                       >  2:102672/1‑140 (MQ=255)                              acacCTTCATTGGTCAACGCCTGCAAATAGGCACTGACCAGACCAACATCGCCGCAGGGCGTTAACTCCGGCAGAACTTCTGCACTGGAAGGCAGGTTCAGACAACCCCCTACTTCTTCCAGTGTTTGCTCGGCTCGCAg                                                                                                       >  2:490944/1‑140 (MQ=255)                              acacCTTCATTGGTCAACGCCTGCAAATAGGCACTGACCAGACCAACATCGCCGCAGGGCGTTAACTCCGGCAGAACTTCTGCACTGGAAGGCAGGTTCAGACAACCCCCTACTTCTTCCAGTGTTTGCTCGGCTCGCAg                                                                                                       >  1:480124/1‑140 (MQ=255)                              acacCTTCATTGGTCAACGCCTGCAAATAGGCACTGACCAGACCAACATCGCCGCAGGGCGTTAACTCCGGCAGAACTTCTGCACTGGAAGACAGGTTCAAACAACCCACTACTTCTGCCAGGGTTTGCTCGGCTCGGAg                                                                                                       >  2:318219/1‑140 (MQ=255)                              acacCTTCATTGGTCAACGCCTGCAAATAGGCACTGACCAGACCAACATCGCCGCAGGGCGTTAACTCCGGCAGAACTTCTGCACTGGAACGCAGGGTCAGACAACCCCCTACTTCTTCCAGTGTTTGCTCGGCCCGCAg                                                                                                       >  2:766918/1‑140 (MQ=255)                                          gTCAACGCCTGCAAATAGGCACTGACCAGACCAACATCGCCGCAGGGCGTTAACTCCGGCAGAACTTCTGCACTGGAAGGCAGGTTCAGACAACCCCCTACTACTTCCAGTGTTGGCACGGCTCTCAGGAACGGGCTCAc                                                                                           >  1:255593/1‑140 (MQ=255)                                                 ccTGCAAATAGGCACTGACCAGACCAACATCGCCGCAGGGCGTTAACTCCGGCAGAACTTCTGCACTGGAAGGCAGGTTCAGACAACCCCCTACTTCTTCCAGTGTTTGCTCGGCTCGCAGGAACGGGCTCACCAGAAcg                                                                                    >  1:17374/1‑140 (MQ=255)                                                    gCAAATAGGCACTGACCAGACCAACATCGCCGCAGGGCGTTAACTCCGGCAGAACTTCTGCACTGGAAGGCAGGTTCAGACAACCCCCTACTTCTTCCAGTGTTTGCTCGGCTCGCAGGAACGGGCTCACCAGAACGCGt                                                                                 <  1:557180/140‑1 (MQ=255)                                                          aGGCACTGACCAGACCAACATCGCCGCAGGGCGTTAACTCCGGCAGAACTTCTGCACTGGAAGGCAGGTTCAGACAACCCCCTACTTCTTCCAGTGTTTGCTCGGCTCGCAGGAACGGGCTCACCAGAACGCGTTCGAtt                                                                           >  1:824388/1‑140 (MQ=255)                                                                  accagaccaACATCGCCGCAGGGCGTTAACTCCGGCAGAACTTCTGCACTGGAAGGCAGGTTCAGACAACCCCCTACTTCTTCCAGTGTTTGCTCGGCTCGCAGGAACGGGCTCACCAGAACGCGTTCGATTTCCACttt                                                                   >  1:860522/1‑140 (MQ=255)                                                                    cagaccaACATCGCCGCAGGGCGTTAACTCCGGCAGAACTTCTGCACTGGAAGGCAGGTTCAGACAACCCCCTACTTCTTCCAGTGTTTGCTCGGCTCGCAGGAACGGGCTCACCAGAACGCGTTCGATTTCCACttttt                                                                 <  2:69667/140‑1 (MQ=255)                                                                     agaccaACATCGCCGCCAGGCGATAACTCCGGCAGAACTCCTGCACTGGAAGGCAGGTTCAGACAACCCCCTACTTCTTCCAGTGTTTGCTCGGCTCGCAGGAACGGGCTCACCAGAACGCGTTCGATTTCCACTTTTTg                                                                <  2:385843/140‑1 (MQ=255)                                                                     agaccaACATCGCCGCAGGGCGTTAACTCCGGCAGAACTTCTGCACTGGAAGGCAGGTTCAGACAACCCCCTACTTCTTCCAGTGTTTGCTCGGCTCGCAGGAACGGGCTCACCAGAACGCGTTCGATTTCCACTTTTTg                                                                <  1:102672/140‑1 (MQ=255)                                                                       accaACATCGCCGCAGGGCGTTAACTCCGGCAGAACTTCTGCACTGGAAGGCAGGTTCAGACAACCCCCTACTTCTTCCAGTGTTTGCTCGCCTCGCAGGAACGGGATCACCAGAACGCGTTCTATTTCCAATTTTTGAc                                                              >  2:395481/1‑140 (MQ=255)                                                                            cATCGCCGCAGGGCGTTAACTCCGGCAGAACTTCTGCACTGGAAGGCAGGTTCAGACAACCCCCTACTTCTTCCAGTGTTTGCTCGGCTCGCAGGAACGGGCTCACCAGAACGCGTTCGATTTCCACTTTTTGACCTTTc                                                         >  2:496618/1‑140 (MQ=255)                                                                             aTCGCCGCAGGGCGTTAACTCCGGCAGAACTTCTGCACTGGAAGGCAGGTTCAGACAACCCCCTACTTCTTCCAGTGTTTGCTCGGCTCGCAGGAACGGGCTCACCAGAACGCGTTCGATTTCCACTTTTTGACCTTTCa                                                        <  2:480124/140‑1 (MQ=255)                                                                              tCGCCGCAGGGCGTTAACTCCGGCAGAACTTCTGCACTGGAAGGCAGGTTCAGACAACCCCCTACTTCTTCCAGTGTTTGCTCGGCTCGCAGGAACGGGCTCACCAGAACACGTACGGTTTCCAGTTTTTGACCTTTCAg                                                       >  1:258582/1‑140 (MQ=255)                                                                                 ccgcAGGGCGTTAACTCCGGCAGAACTTCTGCACTGGAAGGCAGGTTCAGACAACCCCCTACTTCTTCCAGTGTTTGCTCGGCTCGCAGGAACGGGCTCACCAGAACGCGTTCGATTTCCACTTTTTGACCTTTCAGCCa                                                    >  1:588873/1‑140 (MQ=255)                                                                                 ccgcAGGGCGTTAACTCCGGCAGAACTTCTGCACTGGAAGGCAGGTTCAGACAACCCCCTACTTCTTCCAGTGTTTGCTCGGCTCGCAGGAACGGGATCACCAGAACGCGTTCGATTTCCACTTTTTGACCTTTCAGCCa                                                    >  1:202657/1‑140 (MQ=255)                                                                                       ggCGTTAACTCCGGCAGAACTTCTGCACTGGAAGGCAGGTTCAGACAACCCCCTACTTCTTCCAGTGTTTGCTCGGCTCGCAGGAACGGGCTCACCAGAACGCGTTCGATTTCCACTTTTTGACCTTTCAGCCAGt                                                  <  1:166720/136‑1 (MQ=255)                                                                                       ggCGTTAACTCCGGCAGAACTTCTGCACTGGAAGGCAGGTTCAGACAACCCCCTACTTCTTCCAGTGTTTGCTCGGCTCGCAGGAACGGGCTCACCAGAACGCGTTCGATTTCCACTTTTTGACCTTTCAGCCAGt                                                  >  2:166720/1‑136 (MQ=255)                                                                                        gCGTTAACTCCGGCAGAACTTCCGCTCTGAAAGGCAGATTCAGACAACCCCCTACTTCTTCCAGTGTTTGCTCGGCTCGCAGGAACGGGCTCACCAGAACGCGTTCGATTTCCACTTTTTGACCTTTCAGCCAGTTCGcc                                             <  1:318219/140‑1 (MQ=255)                                                                                                   ggCAGAACTTCTGCACTGGAAGGCAGGTTCAGACAACCCCCTACTTCTTCCAGTGTTTGCTCGGCTCGCAGGAACGGGCTCACCAGAACGCGTTCGATTTCCACTTTTTGACCTTTCAGCCAGTTCGCCATCAGGCgaga                                  >  1:181896/1‑140 (MQ=255)                                                                                                   ggCAGAACTTCTGCACTGGAAGGCAGGTTCAGACAACCCCCTACTTCTTCCAGTGTTTGCTCGGCTCGCAGGAACGGGCTCACCAGAACGCGTTCGATTTCCACTTTTTGACCTTTCAGCCAGTTCGCCATCAGGCgaga                                  >  1:720134/1‑140 (MQ=255)                                                                                                           ttCTGCACTGGAAGGCAGGTTCAGACAACCCCCTACTTCTTCCAGTGTTTGCTCGGCTCGCAGGAACGGGCTCACCAGAACGCGTTCGATTTCCACTTTTTGACCTTTCAGCCAGTTCGCCATCAGGCGAGATTCGTcac                          <  2:854396/140‑1 (MQ=255)                                                                                                            tCTGCACTGGAAGGCAGGTTCAGACAACCCCCTACTTCTTCCAGTGTTTGCTCGGCTCGCAGGAACGGGCTCACCAGAACGCGTTCGATTTCCACTTTTTGACCTTTCAGCCAGTTCGCCATCAGGCGAGATTCGTcaca                         <  1:496618/140‑1 (MQ=255)                                                                                                              tGCACTGGAAGGCAGGTTCAGACAACCCCCTCCTTGTTCCAGTGTTTGATCGGCTCGCAGGAACGGGCTCACCAGAACGCGTTCGATTTCCACTTTTTGACCTTTCAGCCAGTTCGCCATCAGGCGAGATTCGTCACAAc                       <  2:258582/140‑1 (MQ=255)                                                                                                              tGCACTGGAAGGCAGGTTCAGACAACCCCCTACTTCTTCCAGTGTTTGCTCGGCTCGCAGGAACGGGCTCACCAGAACGCGTTCGATTTCCACTTTTTGACCTTTCAGCCAGTTAGCCATCAGGCGAGATTCGTca                           >  2:182555/1‑136 (MQ=255)                                                                                                              tGCACTGGAAGGCAGGTTCAGACAACCCCCTACTTCTTCCAGTGTTTGCTCGGCTCGCAGGAACGGGCTCACCAGAACGCGTTCGATTTCCACTTTTTGACCTTTCAGCCAGTTAGCCATCAGGCGAGATTCGTca                           <  1:182555/136‑1 (MQ=255)                                                                                                                  cTGGAAGGCAGGTTCAGACAACCCCCTACTTCTTCCAGTGTTTGCTCGGCTCGCAGGAACGGGCTCACCAGAACGCGTTCGATTTCCACTTTTTGACCTTTCAGCCAGTTCGCCATCAGGCGAGATTCGTCACAACCAtt                   >  1:521593/1‑140 (MQ=255)                                                                                                                    ggAAGGCAGGTTCAGACAACCCCCTCCTTCTTCCAGTGTTTGCTCGGCTCGCAGGAACGGGCTCACCAGAACGCGTTCGATTTCCACTTTTTGACCTTTCAGCCAGTTCGCCATCAGGCGAGATTCGTCACAACCATTAg                 <  1:395481/140‑1 (MQ=255)                                                                                                                     gAAGGCAGGTTCAGACAACCCCCTACTTCTTCCAGTGTTTGCTCGGCTCGCAGGAACGGGCTCACCAGAACGCGTTCGATTTCCACTTTTTGACCTTTCAGCCAGTTCGCCATCAGGCGAGATTCGTCACAACCATTAGt                <  2:720134/140‑1 (MQ=255)                                                                                                                        ggCAGGTTCAGACAACCCCCTACTTCTTCCAGTGTTTGCTCGGCTCGCAGGAACGGGCTCACCAGAACGCGTTCGATTTCCACTTTTTGACCTTTCAGCCAGTTCGCCATCAGGCGAGATTCGTCACAACCATTAGTGGt             <  2:17374/140‑1 (MQ=255)                                                                                                                        gacAGGTTCAGACAACCCCCTACTTCTTCAAGTGTTTCCTCGGCTCGCAGGAACGGGCTCACCAGAACGCGTTCGATTTCCACTTTTTGACCTTTCAGCCAGTTCGCCATCAGGCGAGATTCGTCACAACCATTAGTGGt             <  1:256204/138‑1 (MQ=255)                                                                                                                         gCAGGTTCAGACAACCCTCTACCTCTTCCAGAGTTCGCTCGGCTCGCAAGAACGGGCTCACCAGAACGCGTTCGATTTCCACTTTTTGACCTTTCAGCCAGTTCGCCATCAGGCGAGATTCGTCACAACCATTAGTGGTc            <  2:255593/140‑1 (MQ=255)                                                                                                                              ttCAGACAACCCCCTACTTCTTCCAGTGTTTGCTCGGCTCGCAGGAACGGGCTCACCAGAACGCGTTCGATTTCCACTTTTTGACCTTTCAGCCAGTTCGCCATCAGGCGAGATTCGTCACAACCATTAGTGGTCAGAgg       <  1:490944/140‑1 (MQ=255)                                                                                                                                 aGACAACCCCCTACTTCTTCCAGTGTTTGCTCGGCTCGCAGGAACGGGCTCACCAGAACGCGTTCGATTTCCACTTTTTGACCTTTCAGCCAGTTCGCCATCAGGCGAGATTCGTCACAACCATTAGTGGTCAGAGGACg    >  1:650864/1‑140 (MQ=255)                                                                                                                                 aGACAACCCCCTACTTCTTCCAGTGTTTGCTCGGCTCGCAGGAACGGGCTCACCAGAACGCGTTCGATTTCCACTTTTTGACCTTTCAGCCAGTTCGCCATCAGGCGAGATTCGTCACAACCATTAGTGGTCAGAGGACg    <  2:588873/140‑1 (MQ=255)                                                                                                                                 aGACAACCCCCTACTTCTTCCAGTGTTTGCTCGGCTCGCAGGAACGGGCTCACCAGAACGCGTTCGATTTCCACTTTTTGACCTTTCAGCCAGTTCGCCATCAGGCGAGATTCGCCACAACCATTAGTGGTCAGAGGACg    <  2:181896/140‑1 (MQ=255)                                                                                                                                   aCAACCCCCTACTTCTTCCAGTGTTTGCTCGGCTCGCAGGAACGGGCTCACCAGAACGCGTTCGATTTCCACTTTTTGACCTTTCAGCCAGTTCGCCATCAGGCGAGATTCGTCACAACCATTAGTGGTCAGAGGACGaa  <  2:824388/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                        GTAAGTGAGAGATCACCAGTACCGAAGCAACACCTTCATTGGTCAACGCCTGCAAATAGGCACTGACCAGACCAACATCGCCGCAGGGCGTTAACTCCGGCAGAACTTCTGCACTGGAAGGCAGGTTCAGACAATCCCCTACTTCTTCCAGTGTTTGCTCGGCTCGCAGGAACGGGCTCACCAGAACGCGTTCGATTTCCACTTTTTGACCTTTCAGCCAGTTCGCCATCAGGCGAGATTCGTCACAACCATTAGTGGTCAGAGGACGAA  >  NC_000913/2456485‑2456754

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑