Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,462,324 T→C F340S (TTC→TCC)  fadL → long‑chain fatty acid outer membrane channel/bacteriophage T2 receptor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,462,3240TC100.0% 155.1 / NA 46F340S (TTC→TCC) fadLlong‑chain fatty acid outer membrane channel/bacteriophage T2 receptor
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (22/24);  total (22/24)

AACCTGCCTGAAATGTGGGAAGTGTCAGGTTATAACCGTGTTGATCCACAGTGGGCGATTCACTATAGCCTGGCTTACACCAGCTGGAGTCAGTTCCAGCAGCTGAAAGCGACCTCAACCAGTGGCGACACGCTGTTCCAGAAACATGAAGGCTTTAAAGATGCTTACCGCATCGCGTTGGGTACCACTTATTACTACGATGATAACTGGACCTTCCGTACCGGTATCGCCTTTGATGACAGCCCAGTTCCTGCACAGAATCGTTCTA  >  NC_000913/2462188‑2462455
                                                                                                                                        |                                                                                                                                   
aaCCTGCCTGAAATGTTGGAAGCGTCAGGTTCTGACCGTCTTGATCGAGAGTGGGCGATTCACTATAGCCTGGCTTACACCAGCTGGAGTCAGTTCCAGCAGCTGAAAGCGACCTCAACCAGTGGCGACACGCTGTCCCa                                                                                                                                  <  2:392160/140‑1 (MQ=255)
          aaaTGTGGGAAGTGTCATGTTACAACCGTGTTGATCCACCGTGGGCGAATCACTATAGCCTGGCTTACACCAGCTGGAGTCAGTTCCAGCAGCTGAAAGCGACCTCAACCAGTGGCGACACGCTGTCCCAGAAACATGaa                                                                                                                        <  2:269255/140‑1 (MQ=255)
          aaaTGTGGGAAGTGTCAGGTTATAACCGTGTTGATCCACAGTGGGCGATTCACTATAGCCTGGCTTACACCAGCTGGAGTCAGTTCCAGCAGCTGAAAGCGACCTCAACCAGTGGCGACACGCTGTCCCAGAAACATGaa                                                                                                                        <  1:206222/140‑1 (MQ=255)
                 ggAAGTGTCAGGTTATAACCGTGTTGATCCACAGTGGGCGATTCACTATAGCCTGGCTTACACCAGCTGGAGTCAGTTCCAGCAGCTGAAAGCGACCTCAACCAGTGGCGACACGCTGTCCCAGAAACATGAAGGCTTTa                                                                                                                 >  2:212329/1‑140 (MQ=255)
                         cAGGTTATAACCGTGTTGATCCACAGTGGGCGATTCACTATAGCCTGGCTTACACCAGCTGGAGTCAGTTCCAGCAGCTGAAAGCGACCTCAACCAGTGGCGACACGCTGTCCCAGAAACATGAAGGCTTTAAAGATGCt                                                                                                         >  1:503250/1‑140 (MQ=255)
                                taACCGTGTTGATCCACAGTGGGCGATTCACTATAGCCTGGCTTACACCAGCTGGAGTCAGTTCCAGCAGCTGAAAGCGACCTCAACCAGTGGCGACACGCTGTCCCAGAAACAAGAAGGCTTTAAAGATGCTTACCGCa                                                                                                  >  1:359567/1‑140 (MQ=255)
                                  aCCGTGTTGATCCACAGTGGGCGATTCACTATAGCCTGGCTTACACCAGCTGGAGTCAGTTCCAGCAGCTGAAAGCGACCTCAACCAGTGGCGACACGCTGTCCCAGAAACATGAAGGCTTTAAAGATGCTTACCGCATc                                                                                                <  1:129906/140‑1 (MQ=255)
                                  aCCGTGTTGATCCACAGTGGGCGATTCACTATAGCCTGGCTTACACCAGCTGGAGTCAGTTCCAGCAGCTGAAAGCGACCTCAACCAGTGGCGACACGCTGTCCCAGAAACATGAAGGCTTTAAAGATGCTTACCGCATc                                                                                                <  2:60097/140‑1 (MQ=255)
                                     gtgtTGATCCACAGTGGGCGATTCACTATAGCCTGGCTTACACCAGCTGGAGTCAGTTCCAGCAGCTGAGAGCGACCTCAACCAGTGGCGACACGCTGTCCCAGAAACATGAAGGCTTTAAAGATGCTTACCGCATcgcg                                                                                             <  1:527657/140‑1 (MQ=255)
                                      tgtTGATCCACAGTGGGCGATTCACTATAGCCTGGCTTACACCAGCTGGAGTCAGTTCCAGCAGCTGAAAGCGACCTCAACCAGTGGCGACACGCTGTCCCAGAAACATGAAGGCTTTAAAGATGCTTACCGCATCGCGt                                                                                            >  1:130300/1‑140 (MQ=255)
                                      tgtTGATCCACAGTGGGCGATTCACTATAGCCTGGCTTACACCAGCTGGAGTCAGTTCCAGCAGCTGAAAGCGACCTCAACCAGTGGCGACACGCTGTCCCAGAAACATGAAGGCTTTAAAGATGCTTACCGCATCGCGt                                                                                            >  1:814911/1‑140 (MQ=255)
                                      tgtTGATCCACAGTGGGCAATTCACTATAGCCTGGCTTACACCAGCTGGAGTCAGTTCCAGCAGCTGAAAGCGACCTCAACCAGTGGCGACACGCTGTCCCAGAAACATGAAGGCTTTAAAGATGCTTACCGCATcgcg                                                                                             <  2:830982/139‑1 (MQ=255)
                                      tgtTGATCCACAGTGGGCAATTCACTATAGCCTGGCTTACACCAGCTGGAGTCAGTTCCAGCAGCTGAAAGCGACCTCAACCAGTGGCGACACGCTGTCCCAGAAACATGAAGGCTTTAAAGATGCTTACCGCATcgcg                                                                                             >  1:830982/1‑139 (MQ=255)
                                        ttGATCCACAGTGGGCGATTCACTATAGCCTGGCTTACACCAGCTGGAGTCAGTTCCAGCAGCTGAAAGCGACCTCAACCAGTGGCGACACGCTGTCCTAGAAACATGAAGGCTTTAAAGATGCTTACCGCATCGCGTTg                                                                                          <  2:725822/140‑1 (MQ=255)
                                          gATCCACAGTGGGCGATTCACTATAGCCTGGCTTACACCAGCTGGAGTCAGTTCCAGCAGCTGAAAGCGACCTCAACCAGTGGCGACACGCTGTCCCAGAAACATGAAGGCTTTAAAGATGCTTACCGCATCGCGTTggg                                                                                        <  1:65124/140‑1 (MQ=255)
                                           aTCCACAGTGGGCGATTCACTATAGCCTGGCATACTCCACCTGGAGTCAGTTCCAGCAGCTGAAAGCGACCTCAACCAGTGGCGACACGCTGTCCCAGAAACATGAAGGCTTTAAAGATGCTTACCGCATCGCGTTGGGt                                                                                       <  2:375774/140‑1 (MQ=255)
                                              cacaGTGGGCGATTCACTATAGCCTGGCTTACACCAGCTGGAGTCAGTTCCAGCAGCTGAAAGCGACCTCAACCAGTGGCGACACGCTGTCCCAGAAACATGAAGGCTTTAAAGATGCTTACCGCATCGCGTTGGGTAcc                                                                                    <  2:130300/140‑1 (MQ=255)
                                              cacaGTGGGCGATTCACTATAGCCTGGCTTACACCAGCTGGAGTCAGTTCCAGCAGCTGAAAGCGACCTCAACCAGCAGCGACACGTTGTCCCAGACACATGAAGGCTTTAAAGATGCTTACCGCATCGCGTTGGGTAcc                                                                                    <  2:210117/140‑1 (MQ=255)
                                               acaGTGGGCGATTCACTATAGCCTGGCTTACACCAGCTGGAGTCAGTTCCAGCAGCTGAAAGCGACCTCAACCAGTGGCGACACGCTGTCCCAGAAACATGAAGGCTTTAAAGATGCTTACCGCATCGCGTTGGGTACCa                                                                                   <  1:212329/140‑1 (MQ=255)
                                                     ggCGATTCACTATAGCCTGGCTTACACCAGCTGGAGTCAGTTCCAGCAGCTGAAAGCGACCTCAACCAGTGGCGACACGCTGTCCCAGAAACATGAAGGCTTGAAAGATGGTTATCGAATCGCGATGGGTACCACttatt                                                                             >  1:221239/1‑140 (MQ=255)
                                                             aCTATAGCCTGGCTTACACCAGCTGGAGTCAGTTCCAGCAGCTGAAAGCGACCTCAACCAGTGGCGACACGCTGTCCCAGAAACATGAAGGCTTTAAAGATGCTTACCGCATCGCGTTGGGTACCACTTATTACTACgat                                                                     <  1:861762/140‑1 (MQ=255)
                                                               tataGCCTGGCTTACACCAGCTGGAGTCAGTTCCAGCAGCTGAAAGCGACCTCAACCAGTGGCGACACGCTGTCCCAGAAACATGAAGGCTTTAAAGATGCTTACCGCATCGCGTTGGGTACCACTTATTACTACgatga                                                                   >  1:95831/1‑140 (MQ=255)
                                                                    ccTGGCTTACACCAGCTGGAGTCAGTTCCAGCAGCTGAAAGCGACCTCAACCAGTGGCGACACGCTGTCCCAGAAACATGAAGGCTTTAAAGATGCTTACCGCATCGCGTTGGGTACCACTTATTACTACGATGATAACt                                                              >  1:48156/1‑140 (MQ=255)
                                                                    ccTGGCTTACACCAGCTGGAGTCAGTTCCAGCAGCTGAAAGCGACCTCAACCAGTGGCGACACGCTGTCCCAGAAACATGAAGGCTTTAAAGATGCTTACCGCATCGCGTTGGGTACCACTTATTACTACGATGATAACt                                                              >  2:706530/1‑140 (MQ=255)
                                                                    ccTGGCTTACACCAGCTGGAGTCAGTTCCAGCAGCTGAAAGCGACCTCAACCAGTGGCGACACGCTGTCCCAGAAACATGAAGGCTTTAAAGATCCTTACCGCATCGCGTTGGGTACCACTTATTACTACGATGATAACt                                                              >  2:590809/1‑140 (MQ=255)
                                                                      tGGCTTACACCAGCTGGAGTCAGTTCCAGCAGCTGAAAGCGACCTCAACCAGTGGCGACACGCTGTCCCAGAAACATGAAGGCTTTAAAGATGCTTACCGCATCGCGTTGGGTACCACTTATTACTACGATGATAACTgg                                                            >  2:529791/1‑140 (MQ=255)
                                                                      tGGCTTACACCAGCTGGAGTCAGTTCCAGCAGCTGAAAGCGACCTCAACCAGTGGCGACACGCTGTCCCAGAAACATGAAGGCTTTAAAGAAGCTTACCGCATCACCTTTGGTACCAATTATGCCTACGATGATAACTgg                                                            >  2:401968/1‑140 (MQ=255)
                                                                       ggCTTACACCAGCTGGAGTCAGTTCCAGCAGCTGAAAGCGACCTCAACCAGTGGCGACACGCTGTCCCAGAAACATGAAGGCTTTAAAGATGCTTACCGCATCGCGTTGGGTACCACTTATTACTACGATGATAACTGGa                                                           >  1:760408/1‑140 (MQ=255)
                                                                                  gCTGGAGTCAGTTCCAGCAGCTGAAAGCGACCTCAACCAGTGGCGACACGCTGTCCCAGAAACATGAAGGCTTTAAAGATGCTTACCGCATCGCGTTGGGTACCGCTTTTTACTACGGTGATTACTGGACCTTCCGTAcc                                                >  2:258299/1‑140 (MQ=255)
                                                                                  gCTGGAGTCAGTTCCAGCAGCTGAAAGCGACCTCAACCAGTGGCGACACGCTGTCCCAGAAACATGAAGGCTTTAAAGATGCTTACCGCATCGCGTTGGGGACCACATATTACTACGGTGATAACTGGACCTTCCGTAcc                                                >  2:379741/1‑140 (MQ=255)
                                                                                       aGTCAGTTACAGCAGCTGAAAGCGACCTCAACCAGTGGCGACACGCTGTCCCAGAAACATGAAGGCTTTAAAGATGCTTACCGCATCGCGTTGGGTACCACTTATTACTACGATGATAACTGGACCTTCCGTACCGGTAt                                           >  1:651696/1‑140 (MQ=255)
                                                                                         tCAGTTCCAGCAGCTGACAGCGCCCTCAACCTGTGTCGACACGCTGTCACAGAAACATGAAGGCTTTAAAGATGCTTACCGCATCGCGTTGGGTACCACTTATTACTACGATGATAACTGGACCTTCCGTACCGGTATCg                                         <  2:221239/140‑1 (MQ=255)
                                                                                         tCAGTTCCAGCAGCTGAAAGCGACCTCAACCAGTGGCGACACGCTGTCCCAGAAACATGAAGGCTTTAAAGATGCTTACCGCATCGCGTTGGGTACCACTTATTACTACGATGATAACTGGACCTTCCGTACCGGTATCg                                         <  2:158717/140‑1 (MQ=255)
                                                                                         tCAGTTCCAGCAGCTGAAAGCGACCTCAACCAGTGGCGACACGCTGTCCCAGAAACATGAAGGCTTTAAAGATGCTTACCGCATCGCGTTGGGTACCACTTATTACTACGATGATAACTGGACCTTCCGTACCGGTATCg                                         <  1:590809/140‑1 (MQ=255)
                                                                                            gTTCCAGCAGCTGAACGCGACCGCAGCCAATGGCGACACGCTGTCCCAGAAACATGAAGGCTTTAAAGATGCTTACCGCATCGCGTTGGGTACCACTTATTACTACGATGATAACTGGACCTTCCGTACCGGTATCGCCt                                      <  1:401968/140‑1 (MQ=255)
                                                                                            gTTCCAGCAGCTGAAAGCGACCTCAACCAGTGGCGACACGCTGTCCCAGAAACATGAAGGCTTTAAAGATGCTTACCGCATCGCGTTGGGTACCACTTATTACTACGATGATAACTGGACCTTCCGTACCGGTATCGCCt                                      <  1:529791/140‑1 (MQ=255)
                                                                                            gTTCCAGCAGCTGAAAGCGACCTCAACCAGTGGCGACACGCTGTCCCAGAAACATGAAGGCTTTAAAGATGCTTACCGCATCGCGTTGGGTACCACTTATTACTACGATGATAACTGGACCTTCCGTACCGGTATCGCCt                                      <  1:59989/140‑1 (MQ=255)
                                                                                                cagcagCTGAAAGCGACCTCAACCAGTGGCGACACGCTGTCCCAGAAACATGAAGGCTTTAAAGATGCTTACCGCATCGCGTTGGGTACCACTTATTACTACGATGATAACTGGACCTTCCGTACCGGTATCGCCTTtga                                  >  1:54289/1‑140 (MQ=255)
                                                                                                       tGAAAGCGACCTCAACCAGTGGCGACACGCTGTCCCAGAAACATGAAGGCTTTAAAGATGCTTACCGCATCGCGTTGGGTACCACTTATTACTACGATGATAACTGGACCTTCCGTACCGGTATCGCCTTTGATGACAGc                           >  1:734029/1‑140 (MQ=255)
                                                                                                         aaaGCGACCTCAACCAGTGGCGACACGCTGTCCCAGAAACATGAAGGCTTTAAAGATGCTTACCGCATCGCGTTGGGTACCACTTATTACTACGATGATAACTGGACCTTCCGTACCGGTATCGCCTTTGATGACAGccc                         <  1:697901/140‑1 (MQ=255)
                                                                                                         aaaGCGACCTCAACCAGTGGCGACACGCTGTCCCAGAAACATGAAGGCTTTAAAGATGCTTACCGCATCGCGTTGGGTACCACTTATTACTACGATGATAACTGGACCTTCCGTACCGGTATCGCCTTTGATGACAGccc                         >  2:717518/1‑140 (MQ=255)
                                                                                                               aCCTCAACCAGTGGCGACACGCTGTCCCAGAAACATGAAGGCTTTAAAGATGCTTACCGCATCGCGTTGGGTACCACTTATTACTACGATGATAACTGGACCTTCCGTACCGGTATCGCCTTTGATGACAGCCCAGTTcc                   <  1:379741/140‑1 (MQ=255)
                                                                                                                        aGTGGCGACACGCTGTCACAGAAACATGAAGACTTTAAATATGCTTACTGCATCGCGTTGGGTACCACTTATTACTACGATGATAACTGGACCTTCCGTACCGGTATCGCCTTTGATGACAGCCCAGTTCCTGCACAGaa          <  2:359567/140‑1 (MQ=255)
                                                                                                                          tGGCGACACGCTGTCCCAGAAACATGAAGGCTTTAAAGATGCTTACCGCATCGCGTTGGGTACCACTTATTACTACGATGATAACTGGACCTTCCGTACCGGTATCGCCTTTGATGACAGCCCAGTTCCTGCACAGAATc        >  2:710481/1‑140 (MQ=255)
                                                                                                                           ggCGACACGCTGTCCGAGAAACCTGTAGGGTTTAAAGATGCTTACCGCATCGCGTTGGGTACCACTTATTACTACGATGATAACTGGACCTTCCGTACCGGTATCGCCTTTGATGACAGCCCAGTTCCTGCACAGAATCg       <  1:349327/140‑1 (MQ=255)
                                                                                                                              gACACGCTGTCCCAGAAACATGAAGGCTTTAAAGATGCTTACCGCATCGCGTTGGGTACCACTTATTACTACGATGATAACTGGACCTTCCGTACCGGTAGCGCCTTTGATGACAGCCCAGTTCCTGCACAGAATCGTTc    <  2:503250/140‑1 (MQ=255)
                                                                                                                                cacGCTGTCCCAGAAACATGAAGGCTTTAAAGATGCTTACCGCATCGCGTTGGGTACCACTTATTACTACGATGATAACTGGACCTTCCGTACCGGTATCGCATTTGATGTCAGTCCCGTTCCTACACAGAATCGTTCTa  >  1:363485/1‑140 (MQ=255)
                                                                                                                                        |                                                                                                                                   
AACCTGCCTGAAATGTGGGAAGTGTCAGGTTATAACCGTGTTGATCCACAGTGGGCGATTCACTATAGCCTGGCTTACACCAGCTGGAGTCAGTTCCAGCAGCTGAAAGCGACCTCAACCAGTGGCGACACGCTGTTCCAGAAACATGAAGGCTTTAAAGATGCTTACCGCATCGCGTTGGGTACCACTTATTACTACGATGATAACTGGACCTTCCGTACCGGTATCGCCTTTGATGACAGCCCAGTTCCTGCACAGAATCGTTCTA  >  NC_000913/2462188‑2462455

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑