Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	272,498	T→C	intergenic (+308/‑82)	insN → / → eyeA	CP4‑6 prophage; IS911A regulator fragment/CP4‑6 prophage; small RNA EyeA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	272,498	0	T	C	100.0%	222.8 / NA	63	intergenic (+308/‑82)	insN/eyeA	CP4‑6 prophage; IS911A regulator fragment/CP4‑6 prophage; small RNA EyeA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (30/33);  total (30/33)

ACAACGGGGATTGAGGCGAGATCACTAAGCCGAGATGATCCTCAAGGTTAAGTACTGAAAGGTTGAAGAACATGAACCCGTTAATCCGCCTCTGTGGGTTGAAAACGTCACCACGGCCTACGTGATCTGACAGGCCGTGCAGGAGGAACTGGCAGTGATACGTAAGCACTGCCGGTCGAAGGTGTTTTGACATGTATGCGAAACACCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGATAAAGA  >  NC_000913/272361‑272634                                                                                                                                          |                                                                                                                                         aCAACGGGGATTGAGGCGAGATCACTAAGCCGAGATGATCCTCAAGGTTAAGTACTGAAAGGTTGAAGAACATGAACCCGTTAATCCGCCTCTGTGGGTTGAAAACGTCACCACGGCCTACGTGATCTGACAGGCCgcgc                                                                                                                                        <  2:498790/140‑1 (MQ=255)          aTTGAGGCGAGATCACTAAGCCGAGATGATCCTCAAGGTTCAGTACTGAAAGGTTGAAGAACATGAACCCGTTAATCCGCCTCTGTGGGTTGAAAACGTCACCACGGCCTACGTGATCTGACAGGCCGCGCAGGAGGAAc                                                                                                                               <  2:561517/140‑1 (MQ=255)           ttGAGGCGAGATCACTAAGCCGAGATGATCCTCAAGGTTAAGTACTGAAAGGTTGAAGAACATGAACCCGTTAATCCGCCTCTGTGGGTTGAAAACGTCACCACGGCCTACGTGATCTGACAGGCCGCGCAGGAGGAACt                                                                                                                              >  1:17528/1‑140 (MQ=255)                gCGAGATCACTAAGCCGAGATGATCCTCAAGGTTAAGTACTGAAAGGTTGAAGAACATGAACCCGTTAATCCGCCTCTGTGGGTTGAAAACGTCACCACGGCCTACGTGATCTGACAGGCCGCGCAGGAGGAACTGGCAg                                                                                                                         >  2:514977/1‑140 (MQ=255)                 cGAGATCACTAAGCCGAGATGATCCTCAAGGTTAAGTACTGAAAGGTTGAAGAACATGAACCCGTTAATCCGCCTCTGTGGGTTGAAAACGTCACCACGGCCTACGTGATCTGACAGGCCGCGCAGGAGGAACTGGCAGt                                                                                                                        <  1:2715/140‑1 (MQ=255)                   agaTCACTAAGCCGAGATGATCCTCAAGGTTAAGTACTGAAAGGTTGAAGAACATGAACCCGTTAATCCGCCTCTGTGGGTTGAAAACGTCACCACGGCCTACGTGATCTGACAGGCCGCGCAGGAGGAACTGGCAGTGa                                                                                                                      <  1:578104/140‑1 (MQ=255)                        aCTAAGCCGAGATGATCCTCAAGGTTAAGTACTGAAAGGTTGAAGAACATGAACCCGTTAATCCGCCTCTGTGGGTTGAAAACGTCACCACGGCCTACGTGATCTGACAGGCCGCGCAGGAGGAACTGGCAGTGATAc                                                                                                                   >  2:438521/1‑138 (MQ=255)                        aCTAAGCCGAGATGATCCTCAAGGTTAAGTACTGAAAGGTTGAAGAACATGAACCCGTTAATCCGCCTCTGTGGGTTGAAAACGTCACCACGGCCTACGTGATCTGACAGGCCGCGCAGGAGGAACTGGCAGTGATAc                                                                                                                   <  1:438521/138‑1 (MQ=255)                        aCTAAGCCGAGATGATCCTCAAGGTTAAGTACTGAAAGGTTGAAGAACATGAACCCGTTAATCCGCCTCTGTGGGTTGAAAACGTCACCACGGCCTACGTGATCTGACAGGCCGCGCAGGAGGAACTGGCAGTGATACGt                                                                                                                 <  1:482098/140‑1 (MQ=255)                               cGAGATGATCCTCAAGGTTAAGTACTGAAAGGTTGAAGAACATGAACCCGTTAATCCGCCTCTGTGGGTTGAAAACGTCACCACGGCCTACGTGATCTGACAGGCCGCGCAGGAGGAACTGGCAGTGATACGTAAGCACt                                                                                                          <  2:17528/140‑1 (MQ=255)                               cGAGATGATCCTCAAGGTTAAGTACTGAAAGGTTGAAGAACATGAACCCGTTAATCCGCCTCTGTGGGTTGAAAACGTCACCACGGCCTACCTGATCTGAAAGGACGCGCAGGAGGAACTGGAAGTGATACGTAAGCACt                                                                                                          >  2:335464/1‑140 (MQ=255)                                gagaTGATCCTCAAGGTTAAGTACTGAAAGGTTGAAGAACATGAACCCGTTAATCCGCCTCTGTGGGTTGAAAACGTCACCACGGCCTACGTGATCTGACAGGCCGCGCAGGAGGAACTGGCAGTGATACGTAAGCACTg                                                                                                         <  2:44544/140‑1 (MQ=255)                                 agaTGATCCTCAAGGTTAAGTACTGAAAGGTTGAAGAACATGAACCCGTTAATCCGCCTCTGTGGGTTGAAAACGTCACCACGGCCTACGTGATCTGACAGGCCGCGCAGGAGGAACTGGCAGt                                                                                                                        <  2:509526/124‑1 (MQ=255)                                 agaTGATCCTCAAGGTTAAGTACTGAAAGGTTGAAGAACATGAACCCGTTAATCCGCCTCTGTGGGTTGAAAACGTCACCACGGCCTACGTGATCTGACAGGCCGCGCAGGAGGAACTGGCAGt                                                                                                                        >  1:509526/1‑124 (MQ=255)                                 agaTGATCCTCAAGGTTAAGTACTGAAAGGTTGAAGAACATGAACCCGTTAATCCGCCTCTGTGGGTTGAAAACGTCACCACGGCCTACGTGATCTGACAGGCCGCGCAGGAGGAACTGGCAGTGATACGTAAGCACTGc                                                                                                        >  1:142188/1‑140 (MQ=255)                                 agaTGATCCTCAAGGTTAAGTACTGAAAGGTTGAAGAACATGAACCCGTTAATCCGCCTCTGTGGGTTGAAAACGTCACCACGGCCTACGTGATCTGACAGGCCGCGCAGGAGGAACTGGCAGTGATACGTAAGCACTGc                                                                                                        >  2:182609/1‑140 (MQ=255)                                 agaTGATCCTCAAGGTTAAGTACTGAAAGGTTGAAGAACATGAACCCGTTAATCCGCCTCTGTGGGTTGAAAACGTCACCACGGCCTACGTGATCTGACAGGCCGCGCAGGAGGAACTGGCAGTGATACGTAAGCACTGc                                                                                                        >  1:90996/1‑140 (MQ=255)                                  gatgatCCTCAAGGTTAAGTACTGAAAGGTTGAAGAACATGAACCCGTTAATCCGCCTCTGTGGGTTGAAAACGTCACCACGGCCTACGTGATCTGACAGGCCGCGCAGGAGGAACTGGCAGTGATACGTAAGCACTGcc                                                                                                       <  2:771876/140‑1 (MQ=255)                                   atgatCCTCAAGGTTAAGTACTGAAAGGTTGAAGAACATGAACCCGTTAATCCGCCTCTGTGGGTTGAAAACGTCACCACGGCCTACGTGATCTGACAGGCCGCGCAGGAGGAACTGGCAGTGa                                                                                                                      >  1:673031/1‑124 (MQ=255)                                   atgatCCTCAAGGTTAAGTACTGAAAGGTTGAAGAACATGAACCCGTTAATCCGCCTCTGTGGGTTGAAAACGTCACCACGGCCTACGTGATCTGACAGGCCGCGCAGGAGGAACTGGCAGTGa                                                                                                                      <  2:673031/124‑1 (MQ=255)                                     gatCCTCAAGGTTAACTACTGACAGATTGCAGAACATCAACCCGTTAATCCGCCTCTGTGGGTTGAAAACGTCACCACGGCCTACGTGATCTGACAGGCCGCGCAGGAGGAACTGGCAGTGATACGTAAGCACTGCCGGt                                                                                                    <  1:269784/140‑1 (MQ=255)                                        ccTCAAGGTTAAGTAGTGAAAGTTTGAAGGACATGAAGCCGTTAATCCGCCTCTGTGGGTTGAAAACGTCACCACGGCCTACGTGATCTGACAGGCCGCGCAGGAGGAACTGGCAGTGATACGTAAGCACTGCCGGTCGa                                                                                                 <  1:335464/140‑1 (MQ=255)                                         cTCAAGGTTAAGTACTGAAAGGTTGAAGAACATGAACCCGTTAATCCGCCTCTGTGGGTTGAAAACGTCACCACGGCCTACGTGATCTGACAGGCCGCGCAGGAGGAACTGGCAGTGATACGTAAGCACTGCCGGTCGaa                                                                                                <  2:713745/140‑1 (MQ=255)                                            aaGGTTAAGTACTGAAAGGTTGAAGAACATGAACCCGTTAATCCGCCTCTGTGGGTTGAAAACGTCACCACGGCCTACGTGATCTGACAGGCCGCGCAGGAGGAACTGGCAGTGATACGTAAGCACTGCCGGTCGAAGgt                                                                                             <  2:687506/140‑1 (MQ=255)                                            aaGGTTAAGTACTGAAAGGTTGAAGAACATGAACCCGTTAATCCGCCTCTGTGGGTTGAAAACGTCACCACGGCCTACGTGATCTGACAGGCCGCGCAGGAGGAACTGGCAGTGATACGTAAGCACTGCCGGTCGAAGgt                                                                                             >  1:45225/1‑140 (MQ=255)                                                ttAAGTACTGAAAGGTTGAAGAACATGAACCCGTTAATCCGCCTCTGTGGGTTGAAAACGTCACCACGGCCTACGTGATCTGACAGGCCGCGCAGGAGGAACTGGCAGTGATACGTAAGCACTGCCGGTCGAAGGTGttt                                                                                         <  2:130501/140‑1 (MQ=255)                                                 tAAGTACTGAAAGGTTGAAGAACATGAACCCGTTAATCCGCCTCTGTGGGTTGAAAACGTCACCACGGCCTACGTGATCTGACAGGCCGCGCAGGAGGAACTGGCAGTGATACGTAAGCACTGCCGGTCGAAGGTGtttt                                                                                        >  1:459237/1‑140 (MQ=255)                                                    gTACTGAAAGGTTGAAGAACATGAACCCGTTAATCCGCCTCTGTGGGTTGAAAACGTCACCACGGCCTACGTGATCTGACAGGCCGCGCAGGAGGAACTGGCAGTGATACGTAAGCACTGCCGGTCGAAGGTGTTTTGAc                                                                                     <  2:758890/140‑1 (MQ=255)                                                        tGAAAGGTTGAAGAACATGAACCCGTTAATCCGCCTCTGTGGGTTGAAAACGTCACCACGGCCTACGTGATCTGACAGGCCGCGCAGGAGGAACTGGCAGTGATACGTAAGCACTGCCGGTCGAAGGTGTTTTGACATGt                                                                                 <  2:459237/140‑1 (MQ=255)                                                         gAAAGGTTGAAGAACATGAACCCGTTAATCCGCCTCTGTGGGTTGAAAACGTCACCACGGCCTACGTGATCTGACAGGCCGCGCAGGAGGAACTGGCAGTGATACGTAAGCACTGCCGGTCGAAGGTGTTTTGATATGTa                                                                                <  2:142188/140‑1 (MQ=255)                                                          aaaGGTTGAAGAACATGAACCCGTTAATCCGCCTCTGTGGGTTGAAAACGTCACTACGGCCTACGAGATCTGACAGGCCGCCCAGGAGGAACTGGCAGTGATACGTAAGCACTGCCGGGCGAAGGTGTTCTAACATGTAt                                                                               >  2:810037/1‑140 (MQ=255)                                                              gTTGAAGAACATGAACCCGTTAATCCGCCTCTGTGGGTTGAAAACGTCACCACGGCCTACGTGATCTGACAGGCCGCGCAGGAGGAACTGGCAGTGATACGTAAGCACTGCCGGTCGAAGGTGTTTTGACATGTATGCGa                                                                           <  2:90996/140‑1 (MQ=255)                                                               ttGAAGAACATGAACCCGTTAATCCGCCTCTGTGGGTTGAAAACGTCACCACGGCCTACGTGATCTGACAGGCCGCGCAGGAGGAACTAGCAGTGATACGTAAGCACTGCCGGTCGAAGGTGTTTTGACATGTATGCGaa                                                                          >  1:100177/1‑140 (MQ=255)                                                                 gaagaaCATGAACCCGTTAATCCGCCTCTGTGGGTTGAAAACGTCACCACGGCCTACGTGATCTGACAGGCCGCGCAGGAGGAACTGGCAGTGATACGTAAGCACTGCCGGTCGAAGGTGTTTTGACATGTATGCGAAac                                                                        >  1:4799/1‑140 (MQ=255)                                                                    gaaCATGAACCCGTTAATCCGCCTCTGTGGGTTGAAAACGTCACCACGGCCTACGTGATCTGACAGGCCGCGCAGGAGGAACTGGCAGTGATACGTAAGCACTGCCGGTCGAAGGTGTTTTGACATGTATGCGAAACAcc                                                                     >  2:525879/1‑140 (MQ=255)                                                                         tGAACCCGTTAATCCGCCTCTGTGGGTTGAAAACGTCACCACGGCCTACGTGATCTGACAGGCCGCGCAGGAGGAACTGGCAGTGATACGTAAGCACTGCCGGTCGAAGGTGTTTTGACATGTATGCGAAACACCGGGgc                                                                >  1:65095/1‑140 (MQ=255)                                                                           aaCCCGTTAATCCGCCCGTGTGTGTTGAAAACGCCACCATGGCCTACGCGATCTGACAGGCCGCGCAGGAGGAACTGGCAGTGATACGTAAGCACTGCCGGTCGAAGGTGTTTTGACATGTATGCGAAACACCGGGgcag                                                              <  2:416664/140‑1 (MQ=255)                                                                            aCCCGTTAATCCGCCTCTGTGGGTTGAAAACGTCACCACGGCCTACGTGATCTGACAGGCCGCGCAGGAGGAACTGGGAGTGATACGTAAGCACTGCCGGTCGAAGGTGTTTTGACATGTATGCGAAACACCGGGgcagc                                                             >  2:39986/1‑140 (MQ=255)                                                                               cGTTAATCCGCCTCTGTGGGTTGAAAACGTCACCACGGCCTACGTGATCTGACAGGCCGCGCAGGAGGAACTGGCAGTGATACGTAAGCACTGCCGGTCGAAGGTGTTTTGACATGTATGCGAAACACCGGGGCagcagc                                                          <  2:45225/140‑1 (MQ=255)                                                                                     tCCGCCTCTGTGGGTTGAAAACGTCACCACGGCCTACGTGATCTGACAGGCCGCGCAGGAGGAACTGGCAGTGATACGTAAGCACTGCCGTTCGAAGGTGTTTTGACATGTATGCGAAACACCGGGGCAGCAGCGTCTAt                                                    <  1:810037/140‑1 (MQ=255)                                                                                          ctctGTGGGTTGAAAACGTCACCACGGCCTACGTGATCTGACAGGCCGCGCAGGAGGAACTGGCAGTGATACGTAAGCACTGCCGGTCGAAGGTGTTTTGACATGTATGCGAAACACGGGGGCAGCAGCGTCTATCACGc                                               >  1:322717/1‑140 (MQ=255)                                                                                               tgGGTTGAAAACGTCACCACGGCCTACGTGATCTGACAGGCCGCGCAGGAGGAACTGGCAGTGATACGTAAGCACTGCCGGTCAAAGGTGTTTTGACATGTATGCGAAACACCGGGGCAGCAGCGTCTATCACGCTTGCg                                          >  1:715939/1‑140 (MQ=255)                                                                                                    tGAAAACGTCACCACGGCCTACGTGATCTGACAGGCCGCGCAGGAGGAACTGGCAGTGATACGTAAGCACTGCCGGTCGAAGGTGTTTTGACATGTATGCGAAACACCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCt                                     >  2:856589/1‑140 (MQ=255)                                                                                                      aaaaCGTCACCACGGCCTACGTGATCTGACAGGCCGCGCAGGAGGAACTGGCAGTGATACGTAAGCACTGCCGGTCGAAGGTGTTTTGACATGTATGCGAAACACCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGa                                   <  2:4799/140‑1 (MQ=255)                                                                                                      aaaaCGTCACCACGGCCTACGTGATCTGACAGGCCGCGCAGGAGGAACTGGCAGTGATACGTAAGCACTGCCGGTCGAAGGTGTTTTGACATGTATGCGAAACACCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGa                                   >  1:522547/1‑140 (MQ=255)                                                                                                       aaaCGTCACCACGGCCTACGTGATCTGACAGGCCGCGCAGGAGGAACTGGCAGTGATACGTAAGCACTGCCGGTCGAAGGTGTTTTGACATGTATGCGAAACACCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGAc                                  <  1:514977/140‑1 (MQ=255)                                                                                                           gTCACCACGGCCTACGTGATCTGACAGGCCGCGCAGGAGGAACTGGCAGTGATACGTAAGCACTGCCGGTCGAAGGTGTTTTGACATGTATGCGAAACACCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGACTTCt                              >  1:452635/1‑140 (MQ=255)                                                                                                                       tACGTGATCTGACAGGCCGCGCAGGAGGAACTGGCAGTGATACGTAAGCACTGCCGGTCGAAGGTGTTTTGACATGTATGCGAAACACCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGa                                   >  1:656198/1‑123 (MQ=255)                                                                                                                       tACGTGATCTGACAGGCCGCGCAGGAGGAACTGGCAGTGATACGTAAGCACTGCCGGTCGAAGGTGTTTTGACATGTATGCGAAACACCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGa                                   <  2:656198/123‑1 (MQ=255)                                                                                                                       tACGTGATCTGACAGGCCGCGCAGGAGGAACTGGCAGTGATACGTAAGCACTGCCGGTCGAAGGTGTTTTGACATGTATGCGAAACACCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCgg                  <  1:525879/140‑1 (MQ=255)                                                                                                                         cGTGATCTGACAGGCCGCGCAGGAGGAACTGGCAGTGATACGTAAGCACTGCCGGTCGAAGGTGTTTTGACATGTATGCGAAACACCGGGGTAGCAGCGTCTATAACGGTTGCGTTGATGACTTCTGCCAACTTGCGgca                >  2:363908/1‑140 (MQ=255)                                                                                                                           tGATCTGACAGGCCGCGTAGGATGAACTGGCAGTGGTACCTAAGCACTACCGGTCGAAGGTGTTTTGACATGTATGCGAAACACCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGgcaag              <  2:219855/140‑1 (MQ=255)                                                                                                                           tGATCTGACAGGCCGCGCAGGATGAACTGGCAGTGATACGTAAGCACTGCCGGTCGAAGGTGTTTTGACATGTATGCGAAACACCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGgcaag              <  2:304598/140‑1 (MQ=255)                                                                                                                           tGATCTGACAGGCCGCGCAGGAGGAACTGGCAGTGATACGTAAGCACTGCCGGTCGAAGGTGTTTTGACATGTATGCGAAACACCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGgcaag              <  1:444915/140‑1 (MQ=255)                                                                                                                           tGATCTGACAGGCCGCGAAGGAGGAACTGGCGGTGTTACATAAGCACTCCCGGTCGAAGGTGTTTTGACATGTATGCGAAACACCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGgcaag              <  2:322717/140‑1 (MQ=255)                                                                                                                             aTCTGACAGGCCGCGCAGGAGGAACTGGCAGTGATACGTAAGCACTGCCGGTCGAAGGTGTTTTGACATGTATGCGAAACACCGGGGCAGCAGCGTCTATCAGGCTTGCGGTGCAGAATTCTGCTAACTTGCGgcaagca            >  1:219855/1‑140 (MQ=255)                                                                                                                             aTCTGACAGGCCGCGCAGGAGGAACTGGCAGTGATACGTAAGCACTGCCGGTCGAAGGTGTTTTGACATGTATGCGAAACACCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGgcaagca            <  2:65095/140‑1 (MQ=255)                                                                                                                              tCTGACAGGCCGCGCAGGAGGAACTGGCAGTGATACGTAAGCACTGCCGGTCGAAGGTGTTTTGACATGTATGCGAAACACCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGgcaagcaa           >  1:3959/1‑140 (MQ=255)                                                                                                                               cTGACAGGCCGCGCAGGAGGAACTGGCAGTGATACGTAAGCACTGCCGGTCGAAGGTGTTTTGACATGTATGCGAAACACCGGGGCAGCAGTGTCTATCATGCTCGCGCTGCTGACTTCTGCTAACTTGCGGcaagcaag          >  2:341440/1‑140 (MQ=255)                                                                                                                                 gACAGGCCGCGCAGGAGGAACTGGCAGTGATACGTAAGCACTGCCGGTCGAAGGTGTTTTGACATGTATGCGAAACACCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGa        >  1:671777/1‑140 (MQ=255)                                                                                                                                  aCAGGCCGCGCAGGAGGAACTGGCAGTGATACGTAAGCACTGCCGGTCGAAGGTGTTTTGACATGTATGCGAAACACCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGAt       >  2:737054/1‑140 (MQ=255)                                                                                                                                  aCAGGCCGCGCAGGAGGAACTGGCAGTGATACGTAAGCACTGCCGGTCGAAGGTGTTTTGACATGTATGCGAAACACCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGAt       <  1:856589/140‑1 (MQ=255)                                                                                                                                     ggCCGCGCAGGAGGAACTGGCAGTGATACGTAAGCACTGCCGGTCGAAGGTGTTTTGACATGTATGCGAAACACCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGATaaa    >  1:847140/1‑140 (MQ=255)                                                                                                                                      gCCGCGCAGGAGGAACTGGCAGTGATACGTAAGCACTGCCTGTCGAAGGTGTTTTGACATGTATGCGAAACACCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGATAAag   >  2:544633/1‑140 (MQ=255)                                                                                                                                      gCCGCGCAGGAGGAACTGGCAGTGATACGTAAGCACTGCCGGTCGAAGGTGTTTTGACATGTATGCGAAACACCGGGGCAGCAGCGTCTATCACGCTTGCGTAGCTGACTACTGGCAGCTTGTGGCAAGCAAGGATAAag   >  1:236114/1‑140 (MQ=255)                                                                                                                                       ccGCGCAGGAGGAACTGGCAGTGATACGTAAGCACTGCCGGTCGAAGGTGTTTTGACATGTATGCGAAACACCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGATAAaga  <  2:452635/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                         ACAACGGGGATTGAGGCGAGATCACTAAGCCGAGATGATCCTCAAGGTTAAGTACTGAAAGGTTGAAGAACATGAACCCGTTAATCCGCCTCTGTGGGTTGAAAACGTCACCACGGCCTACGTGATCTGACAGGCCGTGCAGGAGGAACTGGCAGTGATACGTAAGCACTGCCGGTCGAAGGTGTTTTGACATGTATGCGAAACACCGGGGCAGCAGCGTCTATCACGCTTGCGTTGCTGACTTCTGCCAACTTGCGGCAAGCAAGGATAAAGA  >  NC_000913/272361‑272634

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑