Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,504,414 T→C E24E (GAA→GAG)  fryA ← putative PTS multiphosphoryl transfer protein FryA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,504,4140TC100.0% 164.2 / NA 48E24E (GAA→GAG) fryAputative PTS multiphosphoryl transfer protein FryA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (24/24);  total (24/24)

CTGCCGCTAATGTTCAGGCTGCAACTGTCATTAAATAGGGTGCCGGTGCCAATCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGAGAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAA  >  NC_000913/2504277‑2504550
                                                                                                                                         |                                                                                                                                        
cTGCCGCTAATGTTCAGGCTGCAACTGTCATTAAATAGGGTGCCGGTGCCAATCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGCTc                                                                                                                                        >  2:569392/1‑140 (MQ=255)
   ccGCTAATGTTCAGGCTGCAACTGTCATTAAATAGGGTGCCGGTGCCAATCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGCTCttt                                                                                                                                     >  2:465345/1‑140 (MQ=255)
              cAGGCTGCAACTGTCATTAAATAGGGTGCCGGTGCCAATCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCGCTGACTACACTGCGCTTTAAGTTCCCACg                                                                                                                          >  1:287122/1‑140 (MQ=255)
                 gCTGCAACTGTCATTAATTAGGCTGCCGGTGCCAATCAGCGCCAGCGACCTTTTCGCATCTGCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGCTCTTTGAGTTCCCACGCCg                                                                                                                       <  2:229938/140‑1 (MQ=255)
                 gCTGCAACTGTCATTAAATAGGGTGCCGGTGCCAATCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGCTCTTTAAGTTCCCACGCCg                                                                                                                       <  1:619876/140‑1 (MQ=255)
                   tGCAACTGTCATTAAATAGGGTGCCGGTGCCAATCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGCTCTTTAAGTTCCCACGCCGGa                                                                                                                     <  1:607245/140‑1 (MQ=255)
                     cAACTGTCATTAAATAGGGTGCCGGTGCCAATCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTCCCAGTGGGTGCACTGCTCTTTAAGTTCCCACGCCGGACg                                                                                                                   >  2:299401/1‑140 (MQ=255)
                     cAACTGTCATTAAATAGGGTGCCGGTGCCAATCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGAATTCGCTTTGCCATTGGATGCACTGCACTTTAAGTTCCCACGCCGGACg                                                                                                                   >  2:306814/1‑140 (MQ=255)
                      aaCTGTCATTAAATAGGGTGCCGGTGCCAATCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCGCTGGCTGAACTACTCTTTAAGATCCCACGCCGGACGa                                                                                                                  >  1:229938/1‑140 (MQ=255)
                           tCATTAAATAGGGTGCCGGTGCCAATCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGCTCTTTAAGTTCCCACGCCGGACGAGCATg                                                                                                             >  2:555425/1‑140 (MQ=255)
                           tCATTAAATAGGGTGCCGGTGCCAATCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGCTCTTTAAGTTCCCACGCCGGACGAGCATg                                                                                                             >  2:454734/1‑140 (MQ=255)
                           tCATTAAATAGGGTGCCGGTGCCAATCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGCTCTTTAAGTTCCCACGCCGGACGAGCATg                                                                                                             >  1:844479/1‑140 (MQ=255)
                             aTTAAATAGGGTGCCGGTGCCAATCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGCTGTTTGAGTTCCCACACCGGACGAGCATGTa                                                                                                           >  1:222048/1‑140 (MQ=255)
                             aTTAAATAGGGTGCCGGTGCCAATCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGCTCTTTAAGTTCCCACGCCGGACGAGCATGTa                                                                                                           <  2:92207/140‑1 (MQ=255)
                               tAAATAGGGTGCCGGTGCCAATCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGCTCTTTAAGTTCCCACGCCGGACGAGCATGTAGa                                                                                                         >  2:572073/1‑140 (MQ=255)
                                    aGGGTGCCGGTGCCAATCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGCTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGtt                                                                                                    <  2:464751/140‑1 (MQ=255)
                                      ggtgccggtgccAATCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGCTCTTTAAGTTCCCACGCCGGACg                                                                                                                   <  1:123801/123‑1 (MQ=255)
                                      ggtgccggtgccAATCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGCTCTTTAAGTTCCCACGCCGGACg                                                                                                                   >  2:123801/1‑123 (MQ=255)
                                       gtgccggtgccAATCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGCTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAgg                                                                                                 >  2:434239/1‑140 (MQ=255)
                                              tgccAATCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGCTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGa                                                                                          <  1:454734/140‑1 (MQ=255)
                                               gccAATCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGCTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGAc                                                                                         >  2:610963/1‑140 (MQ=255)
                                                        cgcCAGCGAGCTTTTCGCATCTGCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGCTCTTTAAGTTCCCACGCCTGACCAGCGTGTAGACCATTAGCCAAAGGACAGAGAAAtt                                                                                >  2:276573/1‑140 (MQ=255)
                                                           cAGCGAGCTTTTCGCATCTGCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGCTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGAGAAATTGaa                                                                             <  1:40096/140‑1 (MQ=255)
                                                             gCGAGCTTTTCGCATCTGCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGCTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGAGAAATTGAATc                                                                           >  2:212648/1‑140 (MQ=255)
                                                              cGAGCTTTTCGCATCTGCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGCTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGAGAAATTGAATCg                                                                          <  1:434239/140‑1 (MQ=255)
                                                                ggCTTTTCGCATCTGCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCCGCACTGCTCTTTAAGTCCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGAAAAATTGAATCGtt                                                                        <  2:763860/139‑1 (MQ=255)
                                                                aGCTTTTCGCATCTGCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGCTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGAGAAATTGAATCGtt                                                                        >  1:764831/1‑140 (MQ=255)
                                                                      tCGCATCTGCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGCTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGAGAAATTGAATCGTTAACATa                                                                  >  1:800662/1‑140 (MQ=255)
                                                                             tGCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGCTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGAGAAATTGAATCGTTAACATAACCGCTc                                                           <  1:569392/140‑1 (MQ=255)
                                                                               ccTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGCTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGAGAAATTGAATCGTTAACATAACCGCt                                                            >  2:184397/1‑137 (MQ=255)
                                                                               ccTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGCTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGAGAAATTGAATCGTTAACATAACCGCt                                                            <  1:184397/137‑1 (MQ=255)
                                                                                 tttGCGTTCTGGCGATGCTTAACAAAAGTGACTTCTCTTCGCCACTGGCTGCACTGCTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGAGAAATTGAATCGTTAACATAACCGCTCCggg                                                       <  2:222048/140‑1 (MQ=255)
                                                                                  ttGCGTTCTGGCGATTGTTAATGAATGTGTTTTCGCTGTGCCACTGGCTGCACTGCTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGAGAAATTGAATCGTTAACATAACCGCTCCGGGt                                                      <  1:306814/140‑1 (MQ=255)
                                                                                  ttGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGCTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGAGAAATTGAATCGTTAACATAACCGCTCCGGGt                                                      <  2:764831/140‑1 (MQ=255)
                                                                                   tGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGCTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGAGAAATTGAATCGTTAACATAACCGCTCCGGGtt                                                     <  1:527756/140‑1 (MQ=255)
                                                                                         cTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGCTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGAGAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAg                                               >  2:829934/1‑140 (MQ=255)
                                                                                         cTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGCTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGAGAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAg                                               <  1:465345/140‑1 (MQ=255)
                                                                                                            gTGATTTCGCTTTGCCACTGGCTGCACTGCTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGAGAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGt                            >  2:550317/1‑140 (MQ=255)
                                                                                                            gTGATTTCGCTTTGCCACTGGCTGCACTGCTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGAGAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGt                            >  1:452398/1‑140 (MQ=255)
                                                                                                             tGATTTCGCTTTGCCACTGGCTGCAGTGCCCTTTAAGGTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGAGAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTc                           <  1:299401/140‑1 (MQ=255)
                                                                                                               aTTTCGCTTTGCCACTGGCTGCACTGCTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGAGAAATTGAATCGTTAACATAACCACTCCGGGGTAAGCAGGAGATCATCTGAAATCCGTCAg                         >  1:227716/1‑140 (MQ=255)
                                                                                                                 ttCGCTTTGCCACTGGCTGCACTGCTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGAGAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGtt                       >  1:210062/1‑140 (MQ=255)
                                                                                                                    gCTTTGCCACTGGCTGCCCTGCTCTTTAAGTCCCCTCGCCGGACGAGCCTGTAGACCGTTAGGCAAAGGACAGAGAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGaa                    <  2:287122/140‑1 (MQ=255)
                                                                                                                        tGCCACTGGCTGCACTGCTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGAGAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGaacaac                <  1:829934/140‑1 (MQ=255)
                                                                                                                              tGGCTGCACTGCTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGAGAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGtctc          <  1:610963/140‑1 (MQ=255)
                                                                                                                                     aCTGCTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGAGAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTa   <  1:555425/140‑1 (MQ=255)
                                                                                                                                      cTGCTCTTTAAGTTCTCACGCCGGAGGAGAATGTAGATCGTTAGGCAAAGGACAGAGAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTaa  <  1:276573/140‑1 (MQ=255)
                                                                                                                                      cTGCTCTTTAAGTTCCCACGCCGGACGAGCAAGTACACCGTTAGGCAATGGACAGAGAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTaa  <  2:320036/140‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                        
CTGCCGCTAATGTTCAGGCTGCAACTGTCATTAAATAGGGTGCCGGTGCCAATCAGCGCCAGCGAGCTTTTCGCATCTGCCTTTGCGTTCTGGCGATGGTTAATAAAAGTGATTTCGCTTTGCCACTGGCTGCACTGTTCTTTAAGTTCCCACGCCGGACGAGCATGTAGACCGTTAGGCAAAGGACAGAGAAATTGAATCGTTAACATAACCGCTCCGGGTTAATCAGGAGATCATCTGAAATCCGTCAGTTGAACAACCGTCTCACGCGTAA  >  NC_000913/2504277‑2504550

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑