Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,570,274	T→C	T22A (ACG→GCG)	eutJ ←	putative chaperonin, ethanolamine utilization protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,570,274	0	T	C	100.0%	83.4 / NA	28	T22A (ACG→GCG)	eutJ	putative chaperonin, ethanolamine utilization protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (7/21);  total (7/21)

TGCCGTCGCGAACCACATCGGCCCAGTCGAGGCATACCGCCACTGGCTGACCGTCGCGGTCGACAACCATCGACACCACATCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCGGGCGTCTGGTTACACAGCGTTGCCGCCGTTTGCAGACGTGGGGTGAGCCATTGTTCGTCGTGCGCCATCTGTTACTCCTTAAACAATGCGAAACGCATCGACTAATACACAGCGACGCAGACGGACAAACGTACGCGCG  >  NC_000913/2570139‑2570408                                                                                                                                        |                                                                                                                                       tGCCGTCGCGAACCACATCGGCCCAGTCGAGGCATACCGCCACTGGCTGACCGTCGCGGTCGACAACCATCGACACCACATCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCGGGCGCCtcg                                                                                                                                    >  2:644432/1‑138 (MQ=255)                caTCGGCCCAGTCGAGGCATACCGCCACTGGCTGACCGTCGCGGTCGACAACCATCGACACCACATCGCAGGTGCCCAGATCCACCCCCAGTCACAGCGGAGATTCCGACGCGGCGGGCGCCAGGTTACACAGCGTTgcc                                                                                                                     >  2:279901/1‑140 (MQ=255)                 aTCGGCCCAGTCGAGGCATACCGCCACTGGCTGACCGTCGCGGTCGACAACCATCGACACCACATCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCGGGCGCCTGGTTACACAGCGTTgccg                                                                                                                    >  2:13039/1‑140 (MQ=255)                  tCGGCCCAGTCGAGGCATACCGCCACTGGCTGACCGTCGCGGTCGACAACCATCGACACCACATCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCGGGCGCCTGGTTACACAGCGTTgccgc                                                                                                                   <  2:70583/140‑1 (MQ=255)                        cAGTCGAGGCATACCGCCACTGGCTGACCGTCGCGGTCGACAACCATCGACACCACATCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCGGGCGCCTGGTTACACAGCCTTGGCCCCGTTTg                                                                                                             >  2:468132/1‑140 (MQ=255)                          gTCGAGGCATACCGCCACTGGCTGACCGTCGCGGTCGACAACCATCGACACCACATCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCGGGCGCCTGGTTACACAGCGTTGCCGCCGTTTGCa                                                                                                           >  1:198522/1‑140 (MQ=255)                           tCGAGGCATACCGCCACTGGCTGACCGTCGCGGTCGACAACCATCGACACCACATCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCGGGCGCCTGGTTATACAGCGTTGCCGCCGTTTGCAg                                                                                                          >  2:195576/1‑140 (MQ=255)                              gggCATACCGCCACTGGTTGACTGTCGCGGTCGGCCACCGTCGACACCGCATCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCGGGCGCCTGGTTACACAGCGTTGCCGCCGTTTGCAGACg                                                                                                       <  2:420670/139‑1 (MQ=255)                              gggCATACCGCCACTCGCTGACCGTCGCGGTCGACAAGCATCGACACCACATCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCGGGCGCCTGGTTACACAGCGTTGCCGCCGTTTGCAGACg                                                                                                       <  1:382792/139‑1 (MQ=255)                              aGGCATACCGCCACTGGCTGACCGTCGCGGTCGACAACCATCGACACCACATCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCGGGCGCCTGGTTACACAGCGTTGCCGCCGTTTGCAGACg                                                                                                       <  1:796295/140‑1 (MQ=255)                                    aCCGCCACTGGCTGACCGTCGCGGTCGACAACCATCGACACCACATCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCGGGCGCCTGGTCAAACAGCGTTACCGCGTCTTGCAGACGTggggg                                                                                                 >  2:382792/1‑139 (MQ=255)                                     ccGCCACTGGCTGACCGTCGCGGTCGACAACCATCGACACCACATCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCGGGCGCCTGGTTACACAGCGTTGCCGCCGTTTGCAGACGTGGGGTg                                                                                                <  1:605877/140‑1 (MQ=255)                                         cACTGGCTGACCGTCGCGGTCGACAACCATCGACACCACATCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCGGGCGCCTGGTTACACAGCGTTGCCGCCGTTTGCAGACGTGGGGTGAGcc                                                                                            <  2:585191/140‑1 (MQ=255)                                           cTGGCTGACCGTCGCGGTCGACAACCATCGACACCACATCGCCGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCGGGCGCCTGGTTACACAGCGTTGCCGCCGTTTGCAGACg                                                                                                       <  2:731969/127‑1 (MQ=255)                                           cTGGCTGACCGTCGCGGTCGACAACCATCGACACCACATCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCGGGCGCCTGGTTACACAGCGTTGCCGCCGTTTGCAGACg                                                                                                       >  1:731969/1‑127 (MQ=255)                                                        gcgGTCGACAACCATCGACACCACATCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCGGGCGCCTGGTTACACAGCGTTGCCGCCGTTTGCAGACGTGGGGTGAGCCATTGTTCGTCGTgcg                                                                             <  2:159707/140‑1 (MQ=255)                                                        gcgGTCGACAACCATCGACACCACATCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCGGGCGCCTGGTTACACAGCGTTGCCGCCGTTTGCAGACGTGGGGTGAGCCATTGTTCGTCGTgcg                                                                             <  1:468132/140‑1 (MQ=255)                                                          ggTCGACAACCATCGACACCACATCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCGGGCGCCTGGTTACACAGCGTTGCCGCCGTTTGCAGACGTGGGGTGAGCCATTGTTCGTCGTGCGcc                                                                           <  1:37166/140‑1 (MQ=255)                                                           gTCGACAACCATCGACACCACATCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCGGGCGCCTGGTTATACAGCGTTGCCGCCGTTTGCAGACGTGGGGTGAGCCATTGTTCGTCGTGCGCCa                                                                          <  1:195576/140‑1 (MQ=255)                                                              gACAACCATCGACACCACATCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTAGCGGCGGGCGCCCGGGTACACACCGGTTCCGCCGCTCGCCAACGGGGGGGGGGGCATTGTTTGTCGTGCGCCATcc                                                                       >  2:247277/1‑139 (MQ=255)                                                               aCAACCATCGACACCACATCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCGGGCGCCTGGTTACACAGCGTTGCCGCCGTTTGCAGACGTGGGGTGAGCCATTGTTCGTCGTGCGCCATCTg                                                                      <  1:13039/140‑1 (MQ=255)                                                                 aaCCATCGACACCACATCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCGGGCGCCTGGTTACACAGCGTTGCCGCCGTTTGCAGACGTGGGGTGAGCCATTGTTCGTCGTGCGCCATCTGtt                                                                    <  1:824307/140‑1 (MQ=255)                                                                 aaCCATCGACACCACATCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCGGGCGCCTGGTTACACAGCGTTGCCGCCGTTTGCAGACGTGGGGTGAGCCATTGTTCGTCGTGCGCCATCTGtt                                                                    <  1:468689/140‑1 (MQ=255)                                                                 aaCCATCGACACCACATCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCGGGCGCCTGGTTACACAGCGTTGCCGCCGTTTGCAGACGTGGGGTGAGCCATTGTTCGTCGTGCGCCATCTGtt                                                                    <  2:519220/140‑1 (MQ=255)                                                                      tCGACACCACATCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCGGGCGCCTGGTTACACAGGGTCGCCCCCGTTTTCAGACGGGGGGTGCGCCATTGTTCGTCGAGCGCCATCTGTTACTcc                                                               >  2:824307/1‑140 (MQ=255)                                                                             cacaTCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCGGGCGCCTGGTTACACAGCGTTGCCACCGGTTGCAGACGTGGGGTGAGCCATTGTTCGTCGTGCGCCATCTGTTACTCCTTAAACa                                                        >  2:688739/1‑140 (MQ=255)                                                                             cacaTCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCGGGCGCCCGGTTACTCAGCGGTGCCCGCGCATGACGACGCGGGGGGATCCCATGTTCGTCGAGCGCCAGCTGTTTCCCCTTAAACa                                                        >  2:212581/1‑140 (MQ=255)                                                                                       gTGCCCAGATCCACCCCCAGCAACAGCGGAGATTCCGTCGAGGCGGGCGCCTGGCTACAATGCGGTGCCGCCGTCTGAAGTCCTGGGGGGTGCCCTTCTTCGTCGTGTGCCCTTTGGTACTCCTTAACCACTGTGaaaac                                              >  2:759194/1‑138 (MQ=255)                                                                                         gCCCAGATCCACCCCCAGCCACAGCTGAGATTCCGTCGCGGCGGGCGCCTGGTTACACAGCGTTGCCGCCGTTTGCAGACGTGGGGTGAGCCATTGTTCGTCGTGCGCCATCTGTTACTCCTTAAACAATGCGAAACGCa                                            <  1:279901/140‑1 (MQ=255)                                                                                           ccAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCGGGCGCCTGGTTACACAGCGTTGCCGCCGTTTGCAGACGTGGGGTGAGCCATTGTTCGTCGTGCGCCATCTGTTACTCCTTAAACAATGCGAAACGCATc                                          >  2:447043/1‑140 (MQ=255)                                                                                             aGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCGGGCGCCTGGTTACACAGCGTTGCCGCCGTTTGCAGACGTGGGGTGAGCCATTGTTCGTCGTGCGCCATCTGTTACTCCTTAAACAATGCGAAACGCATCGa                                        <  2:44287/140‑1 (MQ=255)                                                                                              gATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCGGGCGCCTGGTTACACAGCGTTGCCGCCGTTTGCAGACGTGGGGTGAGCCATTGTTCGTCGTGCGCCATCTGTTGCTCCTTAAACAATGCGAAACGCATCGAc                                       <  1:759194/140‑1 (MQ=255)                                                                                                                gCGGAGATTCCGTCGCGGCGGGCGCCTGGTTACACAGCGTTGCCGCCGTTTGCAGACGTGGGGTGAGCCATTGTTCGTCGTGCGCCATCTGTTACTCCTTAAACAATGCGAAACGCATCGACTAATACACAGCGACGCAg                     <  2:198522/140‑1 (MQ=255)                                                                                                                                ggcgggcgCCTGGTTACACAGCGTTGCCGCCGTTTGCAGACGTGGGGTGAGCCATTGTTCGTCGTGCGCCATCTGTTACTCCTTAAACAATGCGAAACGCATCGACTAATACACAGCGACGCAGACGGACAAACGTAcgc     <  1:447043/140‑1 (MQ=255)                                                                                                                                   gggcgCCTGGTTTCACAGCGTTGCCGCCGTTTGCAGACGTGGGGTGAGCCATTGTTCGTCGTGCGCCATCTGTTACTCCTTAAACAATGCGAAACGCATCGACTAATACACAGCGACGCAGACGGACAAACGTAcgcgcg  <  2:744602/140‑1 (MQ=255)                                                                                                                                   gggcgCCTGGTTACCCAGCGTTGCCGCCGTTTGCAGACGTCGGGTGAGCCATTGTTCGTCGTGCGCCCTCTGTTACTCCTTAAACAATGCGAAACGCACCGACTAATACACAGCGACGCAGACGGACAAACGTAcgcgcg  <  2:558747/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                       TGCCGTCGCGAACCACATCGGCCCAGTCGAGGCATACCGCCACTGGCTGACCGTCGCGGTCGACAACCATCGACACCACATCGCAGGTGCCCAGATCCACCCCCAGCCACAGCGGAGATTCCGTCGCGGCGGGCGTCTGGTTACACAGCGTTGCCGCCGTTTGCAGACGTGGGGTGAGCCATTGTTCGTCGTGCGCCATCTGTTACTCCTTAAACAATGCGAAACGCATCGACTAATACACAGCGACGCAGACGGACAAACGTACGCGCG  >  NC_000913/2570139‑2570408

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑