BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,638,481	T→C	N58D (AAC→GAC)	bamB ←	outer membrane protein assembly factor BamB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,638,481	0	T	C	100.0%	119.0 / NA	36	N58D (AAC→GAC)	bamB	outer membrane protein assembly factor BamB
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (19/17); total (19/17)

CAGCCATCTTTCTCGGCCAGGCTGACAGACCAGATTTCTTTGCCATCATCCGCATTCAGCGCTTTTACTAAACCAGCGCGGTCCGCTGCATAGACAACGTTGTCCGCCAGTGCCGGATGAAGATTGGAATAGAAGTTGCCAATGCCGCTACCAACGGAAGTGCTCCACGCCGTGGTCGGCGTAAACTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACG  >  NC_000913/2638345‑2638614

cAGCCATCTTTCTCGGCCAGGCTGACAGACCAGATTTCTTTGCCATCATCCGCATTCAGCGCTTTTACTAAACCAGCGCGGTCCGCTGCATAGACAACGTTGTCCGCCAGTGCCGGATGAAGATTGGAATAGAAGTCGcc                                                                                                                                    >  2:175969/1‑140 (MQ=255)

cAGCCATCTTTCTCGGCCAGGCTGACAGACCAGATTTCTTTGCCATCATCCGCATTCAGCGCTTTTACTAAACCAGCGCGGTCCGCTGCATAGACAACGTTGTCCGCCAGTGCCGGATGAAGATTGGAATAGAAGTCGcc                                                                                                                                    >  1:154397/1‑140 (MQ=255)

  gCCATCTTTCTCGGCCAGGCTGACAGACCAGATTTCTTTGCCATCATCCGCATTCAGCGCTTTTACTAAACCAGCGCGGTCCGCTGCATAGACAACGTTGTCCGCCAGTGCCGGATGAAGATTGGAATAGAAGTCGCCaa                                                                                                                                  >  2:92786/1‑140 (MQ=255)

                      tGACAGACCAGATTTCTTTGCCATCATCCGCATTCAGCGCTTTTACTAAACCAGCGCGGTCCGCTGCATAGACAACGTTGTCCGCCAGTGCCGGATGAAGATTGGAATAGAAGTCGCCAATGCCGCTACCAACGGAAGTg                                                                                                              <  2:619126/140‑1 (MQ=255)

                             ccAGATTTCTTTGCCATCATCCGCATTCAGCGCTTTTACTAAACCAGCGCGGTCCGCTGCATAGACAACGTTGTCCGCCAGTGCCGGATGAAGATTGGAATAGAAGTCGCCAATGCCGCTACCAACGGAAGTGCTCCACg                                                                                                       >  2:635473/1‑140 (MQ=255)

                              cAGATTTCTTTGCCATCATCCGCATTCAGCGCTTTTACTAAACCAGCGCGGTCCGCTGCATAGACAACGTTGTCCGCCAGTGCCGGATGAAGATTGGAATAGAAGTCGCCAATGCCGCTACCAACGGAAGTGCTCCACGc                                                                                                      <  1:92464/140‑1 (MQ=255)

                                      tttGCCATCATCCGCATTCAGCGCTTTTACTAAACCAGCGCGGTCCGCTGCATAGACAACGTTGTCCGCCAGTGCCGGATGAAGATTGGAATAGAAGTCGCCAATGCCGCTACCAACGGAAGTGCTCCACGCCGTGGTCg                                                                                              >  1:849931/1‑140 (MQ=255)

                                      tttGCCATCATCCGCATTCAGCGCTTTTACTAAACCAGCGCGGTCCGCTGCATAGACAACGTTGTCCGCCAGTGCCGGATGAAGATTGGAATAGAAGTCGCCAATGCCGCTACCAACGGAAGTGCTCCACGCCGTGGTCg                                                                                              >  1:197935/1‑140 (MQ=255)

                                             tcatcCGCATTCAGCGCTTTTACTAAACCAGCGCGGTCCGCTGCATAGACAACGTTGTTCGCCAGTGCCGGATGAAGATTGGAATAGAAGTCGCCAATGCCGCTACCAACGGAAGTGCTCCACGCCGTGGTCGGCGTaaa                                                                                       <  2:553848/140‑1 (MQ=255)

                                                     aTTCAGCGCTTTTACTAAACCAGCGCGGTCCGCTGCATAGACAACGTTGTCCGCCAGTGCCGGATGAAGATTGGAATAGAAGTCGCCAATGCCGCTACCAACGGAAGTGCTCCACGCCGTGGTCGGCGTAAACTGGtttt                                                                               <  2:197935/140‑1 (MQ=255)

                                                            gcTTTTACTAAACCAGCGCGGTCCGCTGCATAGACAACGTTGTCCGCCAGTGCCGGATGAAGATTGGAATAGAAGTCGCCAATGCCGCTACCAACGGAAGTGCTCCACGCCGTGGTCGGCGTAAACTGGTTTTCAACGGt                                                                        <  1:40402/140‑1 (MQ=255)

                                                              ttttACTAAACCAGCGCGGTCCGCTGCATAGACAACGTTGTCCGCCAGTGCCGGATGAAGATTGGAATAGAAGTCGCCAATGCCGCTACCAACGGAAGTGCTGCACGCCGCGGTCGGAGTAAACAGGTTTTCAACGGTTg                                                                      >  1:413213/1‑140 (MQ=255)

                                                               tttACTAAACAAGCGCGGTCCGCTGCATAGACAACGTTGTCCGCCAGTGCCGGATCAAGATTGGAATAGAAGTCGCCAATGCCGCTACCAACGGAAGTGCTCCACGCCGTGGTCGGCGTAAACTGGTTTTCAACGGTTgg                                                                     <  1:543506/140‑1 (MQ=255)

                                                                 tACTAAACCAGCGCGGTCCGCTGCATAGACAACGTTGTCCGCCAGTGCCGGATGAAGATTGGAATAGAAGTCGCCAATGCCGCTACCAACGGAAGTGCTCCACGCCGTGGTCGGCGTAAACTGGTTTTCAACGGTTGGCa                                                                   <  2:668008/140‑1 (MQ=255)

                                                                 tACTAAACCAGCGCGGTCCGCTGCATAGACAACGTTGTCCGCCAGTGCCGGATGAAGATTGGAATAGAAGTCGCCAATGCCGCTACCAACGGAAGTGCTCCACGCCGTGGTCGGCGTAAACTGGTTTTCAACGGTTGGCa                                                                   <  1:713173/140‑1 (MQ=255)

                                                                        ccAGCGCGGTCCGCTACATAGATAACGTTATCCGCCACTGCCGGATGAAGATTGGAATAGAAGTCGCCAATGCCGCTACCAACGGAAGTGCTCCACGCCGTGGTCGGCGTAAACTGGTTTTCAACGGTTGGCAATGGGGa                                                            <  1:410289/140‑1 (MQ=255)

                                                                            cgcgGTCCGCTGCATAGACAACGTTGTCCGCCAGTGCCGGATGAAGATTGGAATAGAAGTCGCCAATGCCGCTACCAACGGAAGTGCTCCACGCCGTGGTCGGCGTAAACTGGTTTTCAACGGTTGGCAATGGGGACATc                                                        >  2:866068/1‑140 (MQ=255)

                                                                            cgcgGTCCGCTGCATAGACAACGTTGTCCGCCAGTGCCGGATGAAGATTGGAATAGAAGTCGCCAATGCCGCTACCAACGGAAGTGCTCCACGCCGTGGTCGGCATAACCTGGTTTTTAACGGCTGGCAATCGGGACATc                                                        >  2:410289/1‑140 (MQ=255)

                                                                            cgcgGTCCGCTGCATAGACAACGTTGTCCGCCAGTGCCGGATGAAGATTGGAATAGAAGTCGCCAATGCCGCTACCAACGGAAGTGCTCCACGCCGTGGTCGCCGTAAACGGGTTTTAAACGGTAGGCAATGGGGACATc                                                        >  1:313886/1‑140 (MQ=255)

                                                                            cgcgGTCCGCTGCATAGACAACGTTGTCCGCCAGTGCCGGATGAAGATTGGAATAGAAGTCGCCAATGCCGCTACCAACGGAAGTGCTCCACGCCGTGGTCGACGTAAACTGGTATTCAACGGTCGGCAATGGGGACATc                                                        >  1:360816/1‑140 (MQ=255)

                                                                                           aGACAACGTTGTCCGCCAGTGCCGGATGAAGATTGGAATAGAAGTCGCCAATGCCGCTACCAACGGAAGTGCTCCACGCCGTGGTCGGCGTAAACTGGTTTTTAACGGTTCGCATTGGGGACATATTTACCACAtcttct                                         >  1:378941/1‑140 (MQ=255)

                                                                                            gACAACGTTGTCCGCCAGTGCCGGATGAAGATTGGAATAGAAGTCGCCAATGCCGCTACCAACGGAAGTGCTCCACGCCGTGGTCGGCGTAAACTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATcttctt                                        <  1:866068/140‑1 (MQ=255)

                                                                                            gACAACGTTGTCCGCCAGTGCCGGATGAAGATTGGAATAGAAGTCGCCAATGCCGCTACCAACGGAAGTGCTCCACGCCGTGGTCGGCGTAAACTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATcttctt                                        <  2:849931/140‑1 (MQ=255)

                                                                                                  gTTGTCCGCCAGTGCCGGATGAAGATTGGAATAGAAGTCGCCAATGCCGCTACCAACGGAAGTGCTCCACGCCGTGGTCGGCGTAAACTGGTTTTCAACGGTGGGCAATGAGGATATGTTTACCTCATCTTCTTCGCTGt                                  >  1:401283/1‑140 (MQ=255)

                                                                                                      tCCGCCAGTGCCGGATGAAGATTGGAATAGAAGTCGCCAATGCCGCTACCAACGGAAGTGCTCCACGCCGTGGTCGGCGTAAACTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATcttctt                                        <  2:169640/130‑1 (MQ=255)

                                                                                                      tCCGCCAGTGCCGGATGAAGATTGGAATAGAAGTCGCCAATGCCGCTACCAACGGAAGTGCTCCACGCCGTGGTCGGCGTAAACTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATcttctt                                        >  1:169640/1‑130 (MQ=255)

                                                                                                      tCCGCCAGTGCCGGATGAAGATTGGAATAGAAGTCGCCAATGCCGCTACCAACGGAAGTGCTCCACGCCGTGGTCGGCGTAAACTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTaaa                              >  2:685642/1‑140 (MQ=255)

                                                                                                      tCCGCCAGTGCCGGATGAAGATTGGAATAGAAGTCGCCAATGCCGCTACCAACGGAAGTGCTCCACGCCGTGGTCGGCGTAAACTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTaaa                              >  1:551053/1‑140 (MQ=255)

                                                                                                       ccGCCAGTGCCGGATGAAGATTGGAATAGAAGTCGCCAATGCCGCTACCAACGGAAGTGCTCCACGCCGTGGTCGGCGTAAACTGGTTTTCAACGGTTGGCATTGGGGACGTCTATAACACATCATCTTCGCTGTTAAAc                             >  1:357534/1‑140 (MQ=255)

                                                                                                         gCCAGTGCCGGATGAAGATTGGAATAGAAGTCGCCAATGCCGCTACCAACGGAAGTGCTCCACGCCGTGGTCGGCGTAAACTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAg                           >  2:580221/1‑140 (MQ=255)

                                                                                                              tGCCGGATGAAGATTGGCATAGCAGTCGCCACTGCAGCTTCCAACGGACGTGCTCCACGCCGTGGTCGGCGTAAACTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAAc                      <  2:221995/140‑1 (MQ=255)

                                                                                                                ccGGATGAAGATTGGAATAGAAGTCGCCAATGCCGCTACCAACGGAAGTGCTCCACGCCGTGGTCGGCGTAAACTGGTTTTCAACGGTTGGGAATGGGGAAATCGTTAGCACTTCTTATTCGGTGTTAAACAGCGAACAg                    >  2:238162/1‑140 (MQ=255)

                                                                                                                   gATGAAGATTGGAATATCAGTCGCCAATGCCGCTAGCAACGGAAGTGCCCCACGCCGTGGTCGGCGTAAACTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCg                 <  2:378941/140‑1 (MQ=255)

                                                                                                                   gATGAAGATTGGAATAGAAGTCGCCAATGCCGCTACCAACGGAAGTGCTCCACGCCGTGGTCGGCGTAAACTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCg                 <  1:580221/140‑1 (MQ=255)

                                                                                                                              gAATAGAAGTCGCCAATGCCGCTACCAACGGAAGTGCTCCACGCCGTGGTCGGCGTAAACTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGt      <  1:685642/140‑1 (MQ=255)

                                                                                                                                  aGAAGTCGCCAATGCCGCTACCTACGGAAGTACTCAACGCCGTGGTCGCCGTAAACTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACg  <  2:297436/140‑1 (MQ=255)

CAGCCATCTTTCTCGGCCAGGCTGACAGACCAGATTTCTTTGCCATCATCCGCATTCAGCGCTTTTACTAAACCAGCGCGGTCCGCTGCATAGACAACGTTGTCCGCCAGTGCCGGATGAAGATTGGAATAGAAGTTGCCAATGCCGCTACCAACGGAAGTGCTCCACGCCGTGGTCGGCGTAAACTGGTTTTCAACGGTTGGCAATGGGGACATCTTTACCACATCTTCTTCGCTGTTAAACAGCGAACAGCCGCTTAAAAGGGTAACG  >  NC_000913/2638345‑2638614

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`