Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,662,353	A→G	intergenic (‑222/+230)	iscR ← / ← trmJ	DNA‑binding transcriptional dual regulator IscR/tRNA Cm32/Um32 methyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,662,353	0	A	G	100.0%	110.8 / NA	34	intergenic (‑222/+230)	iscR/trmJ	DNA‑binding transcriptional dual regulator IscR/tRNA Cm32/Um32 methyltransferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (16/18);  total (16/18)

TCCCGAGTAAATTGGTCAACTATTTACTTGACTGATTTAGTCGGGTATTTAACCTTCAGTGCCATTTTTTTATCGTGGCGTTTGTACCTGTTGTCGGATGCGGCGTGAACGCCATATCCGCCCTGGGGTTCTGTACACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCA  >  NC_000913/2662217‑2662480                                                                                                                                         |                                                                                                                                tCCCGAGTAAATTGGTCAACTATTTACTTGACTGATTTAGTCGGGTATTTAACCTTCAGTGCCATTTTTTTATCGTGGCGTTTGTACCTGTTGTCGGATGCGGCGTGAACGCCATATCCGCCCTGGGGTTCTGTACGCTg                                                                                                                              <  2:716487/140‑1 (MQ=255)   ccGAGTAAATTGGTCAACTATTTACTTGACTGATTTAGTCGGGTATTTAACCTTCAGTGCCATTTTTTTATCGTGGCGTTTGTACCTGTTGTCGGATGCGGCGTGAACGCCATATCCGCCCTGGGGTTCTGTACGCTGTa                                                                                                                            >  1:73695/1‑140 (MQ=255)     gAGTAAATTGGTCAACTATTTACTTGACTGATTTAGTCGGGTATTTAACCTTCAGTGCCATTTTTTTATCGTGGCGTTTGTACCTGTTGTCGGATGCGGCGTGAACGCCATATCCGCCCTGGGGTTCTGTACGCTGTAgg                                                                                                                          <  1:491851/140‑1 (MQ=255)      aGTAAATTGGTCAACTATTTACTTGACTGATTTAGTCGGGTATTTAACCTTCAGTGCCATTTTTTTATCGTGGCGTTTGTACCTGTTGTCGGATGCGGCGTGAACGCCATATCCGCCCTGGGGTTCTGTACGCTGTAGGc                                                                                                                         <  1:212667/140‑1 (MQ=255)             tGGTCAACTATTTACTTGACTGATTTAGTCGGGTATTTAACCTTCAGTGCCATTTTTTTATCGTGGCGTTTGTACCTGTTGTCGGATGCGGCGTGAACGCCATATCCGCCCTGGGGTTCTGTACGCTGTAGGCCTGATaa                                                                                                                  <  2:189953/140‑1 (MQ=255)                     tATTTACTTGACTGATTTAGTCGGGTATTTAACCTTCAGTGCCATTTTTTTATCGTGGCGTTTGTACCTGTTGTCGGATGCGGCGTGAACGCCATATCCGCCCTGGGGTTCTGTACGCTGTAGGCCTGATAAGACGCAtt                                                                                                          <  1:788561/140‑1 (MQ=255)                              gACTGATTTAGTCGGGTATTTAACCTTCAGTGCCATTTTTTTATCGTGGCGTTTGTACCTGTTGTCGGATGCGGCGTGAACGCCATATCCGCCCTGGGGTTCTGTACGCTGTAGGCCTGATaa                                                                                                                  >  2:733115/1‑123 (MQ=255)                              gACTGATTTAGTCGGGTATTTAACCTTCAGTGCCATTTTTTTATCGTGGCGTTTGTACCTGTTGTCGGATGCGGCGTGAACGCCATATCCGCCCTGGGGTTCTGTACGCTGTAGGCCTGATaa                                                                                                                  <  1:733115/123‑1 (MQ=255)                               aCTGATTTAGTCGGGTATTTAACCTTCAGTGCCATTTTTTTATCGTGGCGTTTGTACCTGTTGTCGGATGCGGCGTGAACGCCATATCCGCCCTGGGGTTCTGTACGCTGTAGGCCTGATAAGACGCATTACGCGTCGCa                                                                                                >  1:434912/1‑140 (MQ=255)                               aCTGATTTAGTCGGGTATTTAACCTTCAGTGCCATTTTTTTATCGTGGCGTTTGTACCTGTTGTCGGATGCGGCGTGAACGCCATATCCGCCCTGGGGTTCTGTACGCTGTAGGCCTGATAAGACGCATTACGCGTCGCa                                                                                                <  1:721472/140‑1 (MQ=255)                               aCTGATTTAGTCGGGCATTTAACCTTCAGTGCCATTTTTTTATCGTGGCGTTTGTACCTGTTGTCGGATGCGGCGTGAACGCCATATCCGCCCTGGGGGTCTGTACGCTGTAGGCCTGATAAGACGCATTACGCGTCGCa                                                                                                >  2:96847/1‑140 (MQ=255)                                cTGATTTAGTCGGGTATTTAACCTTCAGTGCCATTTTTTTATCGTGGCGTTTGTACCTGTTGTCGGATGCGGCGTGAACGCCATATCCGCCCTGGGGTTCTGTACGCTGTAGGCCTGATAAGACGCATTACGCGTCGCAt                                                                                               <  2:572433/140‑1 (MQ=255)                                 tGATTTAGTCGGGTATTTAACCTTCAGTGCCATTTTTTTATCGTGGCGTTTGTACCTGTTGTCGGATGCGGCGTGAACGCCATATCCGCCCTGGGGTTCTGTACGCTGTAGGCCTGATAAGACGCATTACGCGTCGCATc                                                                                              >  2:81960/1‑140 (MQ=255)                                   aTTTAGTCGGGTATTTAACCTTCAGTGCCATTTTTTTATCGTGGCGTTTGTACCTGTTGTCGGATGCGGCGTGAACGCCATATCCGCCCTGGGGTTCTGTACGCTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAg                                                                                            >  1:352196/1‑140 (MQ=255)                                                       ttCAGTGCCATTTTTTTATCGTGGCGTTTGTACCTGTTGTCGGATGCGGCGTGAACGCCATATCCGCCCTGGGGTTCTGTACGCTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATgcgg                                                                        >  1:760761/1‑140 (MQ=255)                                                          aGTGCCATTTTTTTATCGTGGCGTTTGTACCTGTTGTCGGATGCGGCGTGAACGCCATATCCGCCCTGGGGTTCTGTACGCTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGt                                                                     >  1:460788/1‑140 (MQ=255)                                                          aGTGCCATTTTTTTATCGTGGCGTTTGTACCTGTTGTCGGATGCGGCGTGAACGCCATATCCGCCCTGGGGTTCTGTACGCTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGt                                                                     >  2:32035/1‑140 (MQ=255)                                                           gTGCCATTTTTTTATCGTGGCGTTTGTACCTGTTGTCGGATGCGGCGTGAACGCCATATCCGCCCTGGGGTTCTGTACGCTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTg                                                                    >  2:194200/1‑140 (MQ=255)                                                            tGCCATTTTTTTATCGTGGCGTTTGTACCTGTTGTCGGATGCGGCGTGAACGCCATATCCGCCCTGGGGTTCTGTACGCTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGa                                                                   >  1:582680/1‑140 (MQ=255)                                                               cATTTTTTTATCGTGGCGTTTGTACCTGTTGACGGATGCGGCGTGAACGCCATATCCGCCCTGGGGTTCTGTACGCTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACg                                                                <  2:275826/140‑1 (MQ=255)                                                                        aTCGTGGCGTTTGTAGCGGTAGGCGGATGCGTCGTCAGCTCCATATCCACCCTGGGTTTCTGTACGCGGTAGGCCTGATAAGACGCATTGCGCGTCGCATCAGGCAACTGCTGTCGCATGCGGCGTGAACGCTTTATCCg                                                       <  2:298583/140‑1 (MQ=17)                                                                             ggCGTTTGTACCTGTTGTCGGATGCGGCGTGAACGCCATATCCGCCCTGGGGTTCTGTACGCTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTa                                                  <  1:181677/140‑1 (MQ=255)                                                                             ggCGTTTGTACCTGTTGTCGGATGCGGCGTGAACGCCATATCCGCCCTGGGGTTCTGTACGCTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTa                                                  <  2:760761/140‑1 (MQ=255)                                                                             ggCGTTTGTACCTGTTGTCGGATGCGGCGTGAACGCCATATCCGCCCTGGGGTTCTGTACGCTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTa                                                  <  2:434912/140‑1 (MQ=255)                                                                             ggCGTTTGTACCTGTTGTCGGATGCGGCGTGAACGCCATATCCGCCCTGGGGTTCTGTACGCTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTa                                                  <  2:41808/140‑1 (MQ=255)                                                                             ggCGTTTGTACCTGTTGTCGGATGCGGCGTGAACGCCATATCCGCCCTGGGGTTCTGTACGCTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTa                                                  <  1:759497/140‑1 (MQ=255)                                                                             ggCCTTTGTACCTGTTGTCGGTTGCGGTGTGAACCCCATATCCGCCCTGGGGTTCTGTACGCTGTAGGCCTGATAAGCCGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTCTCCGACCTa                                                  <  2:193013/140‑1 (MQ=17)                                                                              gCGTTTGTACCTGTTGTCGGATGCGGCGTGAACGCCATATCCGCCCTGGGGTTCTGTACGCTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTAc                                                 >  1:459481/1‑140 (MQ=255)                                                                                       ccTGTTGTCGGATGCGGCGTGAACGCCATATCCGCCCTGGGGTTCTGTACGCTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGtt                                        <  2:459481/140‑1 (MQ=255)                                                                                       ccTGTTGTCGGATGCGGCGTGAACGCCATATCCGCCCTGGGGTTCTGTACGCTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGtt                                        <  1:194200/140‑1 (MQ=255)                                                                                       ccTGTTGTCGGATGCGGCGTGAACGCCATATCCGCCCTGGGGTTCTGTACGCTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGtt                                        <  1:730011/140‑1 (MQ=255)                                                                                            tgtCGGATGCGGCGTGAACGCCATATCCGCCCTGGGGTTCTGTACGCTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTg                                   >  2:527304/1‑140 (MQ=255)                                                                                                ggATGCGGCGTGAACGCCATATGCGCCCTGGAGTTCTGTCCGCTGTAGTCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAgg                               <  2:352196/140‑1 (MQ=14)                                                                                                        cgTGAACGCCATATCCGCCCTGGGGTTCTGTACGCTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACTGTGCTGTTCACTGTAGGCCTGATaa                       >  1:339919/1‑140 (MQ=255)                                                                                                                         cccTGGGGTTCTGTACGCTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCAGTTCACTGTAGTCCAGATAAGCCGCATTACGCGTTGc      >  1:327902/1‑140 (MQ=11)                                                                                                                             ggggTTCTGTACGCTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCa  >  1:561459/1‑140 (MQ=18)                                                                                                                                         |                                                                                                                                TCCCGAGTAAATTGGTCAACTATTTACTTGACTGATTTAGTCGGGTATTTAACCTTCAGTGCCATTTTTTTATCGTGGCGTTTGTACCTGTTGTCGGATGCGGCGTGAACGCCATATCCGCCCTGGGGTTCTGTACACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCAGGCAACGGCTGTCGGATGCGGCGTGAACGCCTTATCCGACCTACGGTTCTGTTCACTGTAGGCCTGATAAGACGCATTACGCGTCGCATCA  >  NC_000913/2662217‑2662480

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑