Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,710,742	T→C	I108T (ATT→ACT)	nadB →	L‑aspartate oxidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,710,742	0	T	C	100.0%	181.6 / NA	51	I108T (ATT→ACT)	nadB	L‑aspartate oxidase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (26/25);  total (26/25)

CATTGACTCGCATGTGGAAGACACATTGATTGCCGGGGCTGGTATTTGCGATCGCCATGCAGTTGAATTTGTCGCCAGCAATGCACGATCCTGTGTGCAATGGCTAATCGACCAGGGGGTGTTGTTTGATACCCACATTCAACCGAATGGCGAAGAAAGTTACCATCTGACCCGTGAAGGTGGACATAGTCACCGTCGTATTCTTCATGCCGCCGACGCCACCGGTAGAGAAGTAGAAACCACGCTGGTGAGCAAGGCGCTGAACCATC  >  NC_000913/2710605‑2710873                                                                                                                                          |                                                                                                                                    cATTGACTCGCATGTGGAAGACACATTGATTGCCGGGGCTGGTATTTGCGATCGCCATGCAGTTGAATTTGTCGCCAGCAATGCACGATCCGGTGTGCAATGGCTAATGGACCAGGGCGTGTGGTTTGATACCCACACTc                                                                                                                                   >  2:333941/1‑140 (MQ=255)    tGACTCGCATGTGGAAGACACATTGATTGCCGGGGCTGGTATTTGCGATCGCCATGCAGTTGAATTTGTCGCCAGCAATGCACGATCCTGTGTGCAATGGCTAATCGACCAGGGGGTGTTGTTTGATACCCACACTCAAc                                                                                                                                >  2:805133/1‑140 (MQ=255)      aCTCGCATGTGGAAGACACATTGATTGCCGGGGCTGGTATTTGCGATCGCCATGCAGTTGAATTTGTCGCCAGCAATGCACGATCCTGTGTGCAATGGCTAATCGACCAGGGGGTGTTGTTTGATACCCACACTCAACCg                                                                                                                              >  2:819141/1‑140 (MQ=255)             tgtgGAAGACACATTGATTGCCGGGGCTGGTATTTGCGATCGCCATGCAGTTGAATTTGTCGCCAGCAATGCACGATCCTGTGTGCAATGGCTAATCGACCAGGGGGTGTTGTTTGATACCCACACTCAACCGAATGGCg                                                                                                                       <  1:188433/140‑1 (MQ=255)               tgGAAGACACATTGATTGCCGGGGCTGGTATTTGCGATCGCCATGCAGTTGAATTTGTCGCCAGCAATGCACGATCCTGTGTGCAATGGCTAATCGACCAGGGGGTGTTGTTTGATACCCACACTCAACCGAATGGCgaa                                                                                                                     <  2:748403/140‑1 (MQ=255)               tgGAAGACACATTGATTGCCGGCGCCGGTATTTGCGATCGCCATGCAGTTGAATTTGTCGCCAGCAATGCACGATCCTGTGTGCAATGGCTAATCGACCAGGGGGTGTTGTTTGATACCCACACTCAACCGAATGGCgaa                                                                                                                     <  1:333941/140‑1 (MQ=255)                     acacATTGATTGCCGGGGCTGGTATTTGCGATCGCCATGCAGTTGAATTTGTCGCCAGCAATGCACGATCCTGTGTGCAATGGCTAATCGACCAGGGGGTGTTGTTTGATACCCACACTCAACCGAATGGCGAAGAAAGt                                                                                                               >  1:593662/1‑140 (MQ=255)                      cacaTTGATTGCCGGGGCTGGTATTTGCGATCGCCATGCAGTTGAATTTGTCGCCAGCAATGCACGATCCTGTGTGCAATGGCTAATCGACCAGGGGGTGTTGTTTGATACCCACACTCAACCGAATGGCGAAGAAAGtt                                                                                                              >  1:523509/1‑140 (MQ=255)                      cacaTTGATTGCCGGGGCTGGTATTTGCGATCGCCATGCAGTTGAATTTGTCGCCAGCAATGCACGATCCTGTGTGCAATGGCTAATCGACCAGGGGGTGTTGTTTGATACCCACACTCAACCGAATGGCGAAGAAAGtt                                                                                                              <  2:125493/140‑1 (MQ=255)                        caTTGATTGCCGGGGCTGGTATTTGCGATCGCCATGCAGTTGAATTTGTCGCCAGCAATGCACGATCCTGTGTGCAATGGCTAATCGACCAGGGGGTGTTGTTTGATACCCACACTCAACCGAATGGCGAAGAAAGTTAc                                                                                                            <  1:819141/140‑1 (MQ=255)                         attgattgCCGGGGCTGGTATTTGCGATCGCCATGCAGTTGAATTTGTCGCCAGCAATGCACGATCCTGTGTGCAATGGCTAATCGACCAGGGGGTGTTGTTTGATACCCACACTCAACCGAATGGCGAAGAAAGTTAcc                                                                                                           >  2:617463/1‑140 (MQ=255)                         attgattgCCGGGGCTGGTATTTGCGATCGCCATGCAGTTGAATTTGTCGCCAGCAATGCACGATCCTGTGTGCAATGGCTAATCGACCAGGGGGTGTTGTTGGATACCCACACACAGCCGAATAGCGAAGAAAGTTAcc                                                                                                           >  1:218528/1‑140 (MQ=255)                         attgattgCCGGGGCTGGTATTTGCGATCGCCATGCAGTTGAATTTGTCGCCAGCAATGCACGATCCTGTGTGCAATGGCTAATCGACCAGGGGGTGTTGGTTGGTACCCACACTCAACCGAGTGGCGAAGAAAGTTAcc                                                                                                           >  2:336832/1‑140 (MQ=255)                           tgattgCCGGGGCTGGTATTTGCGATCGCCATGCAGTTGAATTTGTCGCCAGCAATGCACGATCCTGTGTGCAATGGCTAATCGACCAGGGGGTGTTGTTTGATACCCACACTCAACCGAATGGCGAAGAAAGTTACCAt                                                                                                         <  1:60722/140‑1 (MQ=255)                              ttgCCGGGGCTGGTATTTGCGATCGCCATGCAGTTGAATTTGTCGCCAGCAATGCACGATCCTGTGTGCAATGGCTAATCGACCAGGGGGTGTTGTTTGATACCCACACTCAACCGAATGGCGAAGAAAGTTACCATCTg                                                                                                      <  2:510460/140‑1 (MQ=255)                                   ggggCTGGTATTTGCGATCGCCATGCAGTTGAATTTGTCGCCAGCAATGCACGATCCTGTGTGCAATGGCTAATCGACCAGGGGGTGTTGTTTGATACCCACACTCAACCGAATGGCGAAGAAAGTTACCATCTGACCCg                                                                                                 >  2:650543/1‑140 (MQ=255)                                   ggggCTGGTATTTGCGATCGCCATGCAGTTGAATTTGTCGCCAGCAATGCACGATCCTGTGTGCAATGGCTAATCGACCAGGGGGTGTTGTTTGATACCCACACTCAACCGAATGGCGAAGAAAGTTACCATCTGACCCg                                                                                                 <  2:588428/140‑1 (MQ=255)                                   ggggCTGGTATTTGCGATCGCCATGCAGTTGAATTTGTCGCCAGCAATGCACGATCCTGTGTGCAATGGCTAATCGACCAGGGGGTGTTGTTTGATACCCACACTCAACCGAATGGCGAAGAAAGTTACCATCTGACCCg                                                                                                 <  2:717225/140‑1 (MQ=255)                                   ggggCTGGTATTTGCGATCGCCATGCAGTTGAATTTGTCGCCAGCAATGCACGATCCTGTGTGCAATGGCTAATCGACCAGGGGGTGTTGTCTGATACCCACACTCAACCGAATGGCCAAGAAAGTTACCATCTGACCCg                                                                                                 >  2:317650/1‑140 (MQ=255)                                        tGGTATTTGCGATCGCCATGCAGTTGAATTTGTCGCCAGCAATGCACGATCCTGTGTGCAATGGCTAATCGACCAGGGGGTGTTGTTTGATACCCACACTCAACCGAATGGCGAAGAAAGTTACCATCTGACCCGTGAAg                                                                                            <  1:617463/140‑1 (MQ=255)                                         ggTATTTGCGATCGCCAAGCAGCTGAATTTGCCGCCAGCAATGCACGAGCCTGTGTGCAATGGCTAATCGACCAGGGGGTGTTGTTTGATACCCACACTCAACCGAATGGCGAAGAAAGTTACCATCTGACCCGTGAAgg                                                                                           <  2:218528/140‑1 (MQ=255)                                           tATTTGCGATCGCCATGCAGTTGAATTTGTCGCCAGCAATGCACGATCCTGTGTGCAATGGCTAATCGACCAGGGGGTGTTGTTTGATACCCACACTCAACCGAATGGCGAAGAAAGTTACCATCTGACCCGTGAAGGTg                                                                                         >  2:843662/1‑140 (MQ=255)                                                    tCGCCATGCAGTTGAATTTGTCGCCAGCAATGCACGATCCTGTGTGCAATGGCTAATCGACCAGGGGGTGTTGTTTGATACCCACACTCAACCGAATGGCGAAGAAAGTTACCATCTGACCCGTGAAGGTGGACATAGTc                                                                                >  2:38814/1‑140 (MQ=255)                                                     cGCCATGCAGTTGAATTTGTCGCCAGCAATGCACGATCCTGTGTGCAATGGCTAATCGACCAGGGGGTGTTGTTTGATACCCACACTCAACCGAATGGCGAAGAAAGTTACCATCTGACCCGTGAAGGTGGACATAGTCa                                                                               <  1:805133/140‑1 (MQ=255)                                                     cGCCATGCAGTTGAATTTGTCGCCAGCAATGCACGATCCTGTGTGCAATGGCTAATCGACCAGGGGGTGTTGTTTGATACCCACACTCAACCGAATGGCGAAGAAAGTTACCATCTGACCCGTGAAGGTGGACATAGTCa                                                                               >  2:191815/1‑140 (MQ=255)                                                                            cAGCAATGCACGATCCTGTGTGCAATGGCTAATCGACCAGGGGGTGTTGTTTGATACCCACACTCAACCGAATGGCGAAGAAAGTTACCATCTGACCCGTGAAGGTGGACATAGTCACCGTCGTATTCTTCATGccgccg                                                        >  2:28525/1‑140 (MQ=255)                                                                                 aTGCACGATCCTGTGTGCAATGGCTAATCGACCAGGGGGTGTTGTTTGATACCCACACTCAACCGAATGGCGAAGAAAGTTACCATCTGACCCGTGAAGGTGGACATAGTCACCGTCGTATTCTTCATGCCGCCGACGcc                                                   <  2:593662/140‑1 (MQ=255)                                                                                       gATCCTGTGTGCAATGGCTAATCGACCAGGGGGTGTTGTTTGATACCCACACTCAACCGAATGGCGAAGAAAGTTACCATCTGACCCGTGAAGGTGGACATAGTCACCGTCGTATTCTTCATGCCGCCGACGCCACCGGt                                             >  2:743244/1‑140 (MQ=255)                                                                                        aTCCTGTGTGCAATGGCTAATCGACCAGGGGGTGTTGTTTGATACCCACACTCAACCGAATGGCGAAGAAAGTTACCATCTGACCCGTGAAGGTGGACATAGTCACCGTCGTATTCTTCATGCCGCCGACGCCACCGGTa                                            <  1:191815/140‑1 (MQ=255)                                                                                        aTCCTGTGCGCAATGGCTAATCGACCAGGGGGTGTTGTTTGATACCCACACTCAACCGAATGGCGAAGAAAGTTACCATCTGACCCGTGAAGGTGGACATAGTCACCGTCGTATTCTTCATGCCGCCGACGCCACCGGTa                                            <  2:523509/140‑1 (MQ=255)                                                                                          ccTGTGTGCAATGGCTAATCGAGCATGGGGTGTTGTTTGATACCCACACTCAACCGAATGGCGAAGAAAGTTACCATCTGACCCGTGAAGGTGGACATAGTCACCGTCGTATTCTTCATGCCGCCGACGCCACCGGTaga                                          <  1:336832/140‑1 (MQ=255)                                                                                          ccTGTGTGCAATGGCTAATCGACCAGGGGGTGTTGTTTGATACCCACACTCAACCGAATGGCGAAGAAAGTTACCATCTGACCCGTGAAGGTGGACATAGTCACCGTCGTATTCTTCATGCCGCCGACGCCACCGGTaga                                          <  2:191080/140‑1 (MQ=255)                                                                                            tgtgtgCAATGGCTAATCGACCAGGGGGTGTTGTTTGATACCCACACTCAACCGAATGGCGAAGAAAGTTACCATCTGACCCGTGAAGGTGGACATAGTCACCGTCGTATTCTTCATGCCGCCGACGCCACCGGTAgaga                                        >  2:495650/1‑140 (MQ=255)                                                                                                   aaTGGCTAATCGACTAGGGGGTGTTGTTTGATACCCACACTCAACCGAATGGCGAAGAAAGTTACCATCTGACCCGTGAAGGTGGACATAGTCACCGTCGTATTCTTCATGCCGCCGACGCCACCGGTAGAGAAGTAGaa                                 >  1:652445/1‑140 (MQ=255)                                                                                                   aaTGGCTAATCGACCAGGGGGTGTTGTTTGATACCCACACTCAACCGAATGGCGAAGAAAGTTACCATCTGACCCGTGAAGGTGGACATAGTCACCGTCGTATTCTTCATGCCGCCGACGcc                                                   >  2:8774/1‑122 (MQ=255)                                                                                                   aaTGGCTAATCGACCAGGGGGTGTTGTTTGATACCCACACTCAACCGAATGGCGAAGAAAGTTACCATCTGACCCGTGAAGGTGGACATAGTCACCGTCGTATTCTTCATGCCGCCGACGcc                                                   <  1:8774/122‑1 (MQ=255)                                                                                                   aaTGGCTAATCGACCAGGGGGTGTTGTTTGATACCCACACTCAACCGAATGGCGAAGAAAGTTACCATCTGACCCGTGAAGGTGGACATAGTCACCGTCGTATTCTTCATGCCGCCGACGCCAc                                                 >  1:193464/1‑124 (MQ=255)                                                                                                   aaTGGCTAATCGACCAGGGGGTGTTGTTTGATACCCACACTCAACCGAATGGCGAAGAAAGTTACCATCTGACCCGTGAAGGTGGACATAGTCACCGTCGTATTCTTCATGCCGCCGACGCCAc                                                 <  2:193464/124‑1 (MQ=255)                                                                                                   aaTGGCTAATCGACCAGGGGGTGTTGTTTGATACCCACACTCAACCGAATGGCGAAGAAAGTTACCATCTGACCCGTGAAGGTGGACATAGTCACCGTCGTATTCTTCATGCCGCCGACGCCACCGGTAGAGAAGTAGaa                                 >  2:750532/1‑140 (MQ=255)                                                                                                   aaTGGCTAATCGACCAGGGGGTGTTGTTTGATACCCACACTCAACCGAATGACGAAGAAAGCTACCATCTGACCCGTGAAGGTGGACCTACGCTCCGTCGGATTATTCTTGCCGCCGGCGCCTTCGGTAGAGAAGGAGaa                                 >  2:388860/1‑140 (MQ=255)                                                                                                       gCTAATCGACCAGGGGGTGTTGTTTGATACCCACACTCAACCGAATGGCGAAGAAAGTTACCATCTGACCCGTGAAGGTGGACATAGTCACCGTCGTATTCTTCATGCCGCCGACGCCACCGGTAGAGAAGTAGAAACCa                             <  1:650543/140‑1 (MQ=255)                                                                                                        cTAATCGACCAGGGGGTGTTGTTTGATACCCACACTCAACCGAATGGCGAAGAAAGTTACCATCTGACCCGTGAAGGTGGACATAGTCACCGTCGTATTCTTCATGCCGCCGACGCCACCGGTAGAGAAGTAGAAACCAc                            >  1:690092/1‑140 (MQ=255)                                                                                                         tAATCGACCAGGGGGTGTTGTTTGATACCCACACTCAACCGAATGGCGAAGAAAGTTACCATCTGACCCGTGAAGGTGGACATAGTCACCGTCGTATTCTTCATGCCGCCGACGCCACCGGTAGAGAAGTAGAAACCACg                           <  2:690092/140‑1 (MQ=255)                                                                                                                   gggggTGTTGTTTGATACCCACACTCAACCGAATGGCGAAGAAAGTTACCATCTGACCCGTGAAGGTGGACATAGTCACCGTCGTATTCTTCATGCCGCCGACGCCACCGGTAGAGAAGTAGAAACCACGCTGGTGAGCa                 <  1:750532/140‑1 (MQ=255)                                                                                                                   gggggTGTTGTTTGATACCCACACTCAACCGAATGGCGAAGAAAGTTACCATCTGACCCGTGAAGGTGGACATAGTCACCGTCGTATTCTTCATGCCGCCGACGCCACCGGTAGAGAAGTAGAAACCACGCTGGTGAGCa                 <  2:134970/140‑1 (MQ=255)                                                                                                                          ttgttTGATACCCACACTCAACCGAATGGCGAAGAAAGTTACCATCTGACCCGTGAAGGTGGACATAGTCACCGTCGTATTCTTCATGCCGCCGACGCCACCGGTAGAGAAGTAGAAACCACGCTGGTGAGCAAGGCGCt          >  2:73922/1‑140 (MQ=255)                                                                                                                             tttGATACCCACACTCAACCGAATGGCGAAGAAAGTTACCATCTGACCCGTGAAGGTGGACATAGTCACCGTCGTATTCTTCATGCCGCCGACGCCACCGGTAGAGAAGTAGAAACCACGCTGGTGAGCAAGGCGCTGaa       <  1:28525/140‑1 (MQ=255)                                                                                                                                  tACCCACACTCAACCGAATGGCGAAGAAAGTTACCATCTGACCCGTGAAGGTGGACATAGTCACCGTCGTATTCTTCATGCCGCCGACGCCACCGGTAGAGAAGTAGAAACCACGCTGGTGAGCAAGgcg            <  2:450068/130‑1 (MQ=255)                                                                                                                                  tACCCACACTCAACCGAATGGCGAAGAAAGTTACCATCTGACCCGTGAAGGTGGACATAGTCACCGTCGTATTCTTCATGCCGCCGACGCCACCGGTAGAGAAGTAGAAACCACGCTGGTGAGCAAGgcg            >  1:450068/1‑130 (MQ=255)                                                                                                                                  tACCCACACTCAACCGAATGGCGAAGAAAGTTACCATCTGACCCGTGAAGGTGGACATAGTCACCGTCGTATTCTTCATGCCGCCGACGCCACCGGTAGAGAAGTAGAAACCACGCTGGTGAGCAAGGCGCTGAACCATc  >  2:126779/1‑140 (MQ=255)                                                                                                                                  tACCCACACTCAACCGAATGGCGAAGAAAGTTACCATCTGACCCGTGAAGGTGGACATAGTCACCGTCGTATTCTTCATGCCGCCGACGCCACCGGTAGAGAAGTAGAAACCACGCTGGTGAGCAAGGCGCTGAACCATc  >  2:450139/1‑140 (MQ=255)                                                                                                                                  tACCCACACTCAACCGAATGGCGAAGAAAGTTACCATCTGACCCGTGAAGGTGGACATAGTCACCGTCGTATTCTTCATGCCGCCGACGCCACCGGTAGAGAAGTAGAAACCACGCTGGTGAGCAAGGCGCTGAACCATc  >  1:791772/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                    CATTGACTCGCATGTGGAAGACACATTGATTGCCGGGGCTGGTATTTGCGATCGCCATGCAGTTGAATTTGTCGCCAGCAATGCACGATCCTGTGTGCAATGGCTAATCGACCAGGGGGTGTTGTTTGATACCCACATTCAACCGAATGGCGAAGAAAGTTACCATCTGACCCGTGAAGGTGGACATAGTCACCGTCGTATTCTTCATGCCGCCGACGCCACCGGTAGAGAAGTAGAAACCACGCTGGTGAGCAAGGCGCTGAACCATC  >  NC_000913/2710605‑2710873

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑