Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,723,976	A→G	H417R (CAC→CGC)	pssA →	phosphatidylserine synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,723,976	0	A	G	100.0%	154.0 / NA	45	H417R (CAC→CGC)	pssA	phosphatidylserine synthase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (21/24);  total (21/24)

ATAACCTGAACCCGCGCGCCTGGCGTCTGGATCTGGAAAACGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCACAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCACTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTG  >  NC_000913/2723844‑2724109                                                                                                                                     |                                                                                                                                      ataaCCTGAACCCGCGCGCCTGGCGTCTGGATCTGGAAAACGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCACAGCGAGAGAAAGAACTGGAGCAGATCCGCTAGCGTAGCACCATCGTTAAGCGCTATcgc                                                                                                                                >  1:270550/1‑140 (MQ=255)  taaCCTGAACCCGCGCGCCTGGCGTCTGGATCTGGAAAACGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCACAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCGCTATcgcg                                                                                                                               <  1:837468/140‑1 (MQ=255)  taaCCTGAACCCGCGCGCCTGGCGTCTGGATCTGGAAAACGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCACAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCGCTATcgcg                                                                                                                               <  1:818812/140‑1 (MQ=255)   aaCCTGAACCCGCGCGCCTGGCGTCTGGATCTGGAAAACGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCACAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCGCTATCGCGa                                                                                                                              <  1:535862/140‑1 (MQ=255)    aCCTGAACCCGCGCGTCTGGCGCCTCGATCTGGAAAACACCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCACAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCGCTATCGCGAt                                                                                                                             <  1:329368/140‑1 (MQ=255)         aaCCCGCGCGCCTGGCGTCTGGATCTGGAAAACGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCACAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCGCTATCGCGATCTGCa                                                                                                                        >  1:187977/1‑140 (MQ=255)         aaCCCGCGCGCCTGGCGTCTGGATCTGGAAAACGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCACAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCGCTATCGCGATCTGCa                                                                                                                        >  2:468494/1‑140 (MQ=255)         aaCCCGCGCGCCTGGCGTCTGGATCTGGAAAACGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCACAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCGCTATCGCGATCTGCa                                                                                                                        <  2:468994/140‑1 (MQ=255)         aaCCCGCGCGCCTGGCGGCTGGCTCTGGAAACCGCTATTATGATCCACTATCCGCAACTTGAGCTGGCGCCACAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCGCTATCGCGATCTGCa                                                                                                                        <  2:270550/140‑1 (MQ=255)              gcgcgcCTGGCGTCTGGATCTGGAAAACGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCACAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCGCTATCGCGATCTGCAAAGTa                                                                                                                   >  1:505926/1‑140 (MQ=255)              gcgcgcCTGGCGTCTGGATCTGGAAAACGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCACAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCGCTATCGCGATCTGCAAAGTa                                                                                                                   >  1:590693/1‑140 (MQ=255)              gcgcgcCTGGCGTCTGGATCTGGAAAACGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCACAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCGCTATCGCGATCTGCAAAGTa                                                                                                                   >  1:34072/1‑140 (MQ=255)               cgcgcCTGGCGTCTGGATCTGGAAAACGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCACAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCGCTATcgcg                                                                                                                               <  2:709070/127‑1 (MQ=255)               cgcgcCTGGCGTCTGGATCTGGAAAACGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCACAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCGCTATcgcg                                                                                                                               >  1:709070/1‑127 (MQ=255)                gcgcCTGGCGTCTGGATCTGGAAAACGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCACAGCGAGAGAAAGAACTGGAGCTGATACGCGAGCAAACCGCCAGGGTTAAGCGCTATCACGATCTGGAAAGTAtt                                                                                                                 >  2:422148/1‑140 (MQ=255)                    cTGGCGTCTGGATCTGGAAAACGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCACAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCGCTATCGCGATCTGCAAAGTATTGCCg                                                                                                             >  1:801853/1‑140 (MQ=255)                          tctggatctggaAAACGCCATTGTGCTCCCCGATCCGCAACTTGAGCTGGCGCCACAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCGCTATCGCGATCTGCAAAGTATTGCCGATTATc                                                                                                       <  1:287385/140‑1 (MQ=255)                              gatctggaAAACGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCACAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCCCCATCGTTAAGCGCTATCGCGATCGGCAAAGTATTGCCGATTATCCGGt                                                                                                   <  1:32250/140‑1 (MQ=255)                               atctggaAAACGCCATTTTGATCCACGATCCGGAACTTGAGCTGGCGCCACAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCGCTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTg                                                                                                  <  2:590693/140‑1 (MQ=255)                               atctggaAAACGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCACAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCGCTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTg                                                                                                  <  2:505926/140‑1 (MQ=255)                                 ctggaAAACGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCACAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCGCTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGaa                                                                                                <  1:510647/140‑1 (MQ=255)                                 ctggaAAACGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCACAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCGCTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGaa                                                                                                <  1:689925/140‑1 (MQ=255)                                  tggaAAACGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCACAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCGCTATCGCGATCTGCAAAGTATTGCCGATTATc                                                                                                       <  1:208892/132‑1 (MQ=255)                                  tggaAAACGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCACAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCGCTATCGCGATCTGCAAAGTATTGCCGATTATc                                                                                                       >  2:208892/1‑132 (MQ=255)                                   ggaAAACGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCACAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCGCTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAgg                                                                                              <  2:801853/140‑1 (MQ=255)                                         cGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCACAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCGCTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTa                                                                                        >  2:749669/1‑140 (MQ=255)                                                   aTCCACGATCCGCAACTTGAGCTGGCGCCACAGAGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCGCTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATccg                                                                              <  1:468494/140‑1 (MQ=255)                                                        cGATCCGCAACTTGAGCTGGCGCCACAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCGCTATCGCGATCTGCAAAGTATTGCAGATTATCTGGTGAAGGTTCGTTAACTCATCCGCCGtt                                                                         >  1:357486/1‑140 (MQ=255)                                                         gATCCGCACCTTGAGCTGGCGCCACAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCCCCATCGTTAAGCGCTATCGCGATGTGCAAAGTATGGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGttt                                                                        <  2:766788/140‑1 (MQ=255)                                                           tCCGCAACTTGAGCTGGCGCCACAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCGCTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTgc                                                                      <  2:34072/140‑1 (MQ=255)                                                                     gAGCTGGCGCCACAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCGCTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCg                                                            >  1:437442/1‑140 (MQ=255)                                                                         tGGCGCCACAGCGAGAGAAAGACCTGGAGCTCATCCGCGTGCATACCACCATCGTTAAGCGCTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATc                                                        <  2:357486/140‑1 (MQ=255)                                                                            cgcCACAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGTATACCACCATCGTTAAGCGCTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGAc                                                     >  2:667219/1‑140 (MQ=255)                                                                             gcCACAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCGCTATCGCGATCTGCAAAGTATTGCCGATTATCCGATGAAGGTTCGTACACTAATCCGCCGATTGCCCCGTATCCGCATCGAcc                                                    >  2:330902/1‑140 (MQ=255)                                                                                    cGAGAGAAAGAACTGGAGCTGATCCGCGAGTATACCACCATCGTTAAGCGCTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGattaat                                             <  1:667219/140‑1 (MQ=255)                                                                                                 tGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCGCTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTCCGCGGTATCCGCATCGACCGATTAATTAGCCGCATCCTg                                >  2:287866/1‑140 (MQ=255)                                                                                                       tGATCCGCGAGCATACCACCATCGTTAAGCGCTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATca                          <  2:187977/140‑1 (MQ=255)                                                                                                       tGATCCGCGAGCATACCACCATCGTTAAGCGCTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATca                          <  2:507183/140‑1 (MQ=255)                                                                                                        gATCCGCGAGCATACCACCATCGTTAAGCGCTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTGTCCACATTGACCGATTTATTAGCCGCATCCTGTAATcac                         >  2:260176/1‑140 (MQ=255)                                                                                                         aTCCGCGAGCATACCACCATCGTTAAGCGCTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATcaca                        >  2:722701/1‑140 (MQ=255)                                                                                                            cgcgAGCATACCACCATCGTTAAGCGCTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAAcc                     <  1:722701/140‑1 (MQ=255)                                                                                                             gcgAGCATACCACCATCGTTAAGCGCTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAAccc                    <  2:437442/140‑1 (MQ=255)                                                                                                                           aTCGTTAAGCGCTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACgggg      >  1:481385/1‑140 (MQ=255)                                                                                                                            tCGTTAAGCGCTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGgt     >  2:163403/1‑140 (MQ=255)                                                                                                                              gTTAAGCGCTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGgttt   >  2:742426/1‑140 (MQ=255)                                                                                                                               ttAAGCGCTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGgtttg  >  1:172093/1‑140 (MQ=255)                                                                                                                                     |                                                                                                                                      ATAACCTGAACCCGCGCGCCTGGCGTCTGGATCTGGAAAACGCCATTTTGATCCACGATCCGCAACTTGAGCTGGCGCCACAGCGAGAGAAAGAACTGGAGCTGATCCGCGAGCATACCACCATCGTTAAGCACTATCGCGATCTGCAAAGTATTGCCGATTATCCGGTGAAGGTTCGTAAACTCATCCGCCGTTTGCGCCGTATCCGCATCGACCGATTAATTAGCCGCATCCTGTAATCACAACCCCGTCCTGTACGGGGTTTG  >  NC_000913/2723844‑2724109

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑