Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,877,685	T→C	intergenic (+67/+884)	iap → / ← cas2	alkaline phosphatase isozyme conversion protein/CRISPR‑associated endoribonuclease Cas2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,877,685	0	T	C	100.0%	39.7 / NA	13	intergenic (+67/+884)	iap/cas2	alkaline phosphatase isozyme conversion protein/CRISPR‑associated endoribonuclease Cas2
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (2/11);  total (2/11)

CGCTGCCGTGACGTTATGCGGATAATGCTACCTCTGGTGAAGGAGTTGGCGAAGGCGTCTTGATGGGTTTGAAAATGGGAGCTGGGAGTTCTACCGCAGAGGCGGGGGAACTCCAAGTGATATCCATCATCGCATCCAGTGCGCCCGGTTTATCCCCGCTGATGCGGGGAACACCAGCGTCAGGCGTGAAATCTCACCGTCGTTGCCGGTTTATCCCTGCTGGCGCGGGGAAC  >  NC_000913/2877556‑2877788                                                                                                                                  |                                                                                                        cGCTGCCGTGACGTTATGCGGATAATGCTACCTCTGGTGAAGGAGTTGGCGAAGGCGTCTTGATGGGTTTGAAAATGGGAGCTGGGAGTTCGACCGCAGACGCGGGGGAACTCCAAAAGATAACCAACACCGCATCCAGt                                                                                               >  2:236414/1‑140 (MQ=255)    tGCCGTGACGTTATGCGGATAATGCTACCTCTGGTGAAGGAGTTGGCGAAGGCGTCTTGATGGGTTTGAAAATGGGAGCTGGGAGTTCTACCGCAGAGGCGGGGGAACTCCAAGTGATATCCATCACCGCATCCAGTgcg                                                                                            <  2:112957/140‑1 (MQ=255)     gCCGTGACGTTATGCGGATAATGCTACCTCTGGTGAAGGAGTTGGCGAAGGCGTCTTGATGGGTTTGAAAATGGGAGCTGGGAGTTCTACCGCAGAGGCGGGGGAACTCCAAGTGATATCCATCACCGCATCCAGTgcgc                                                                                           <  1:455061/140‑1 (MQ=255)      ccGTGACGTTATGCGGATAATGCTACCTCTGGTGAAGGAGTTGGCGAAGGCGTCTTGATGGGTTTGAAAATGGGAGCTGGGAGTTCTACCGCAGAGGCGGGGGAACTCCAAGTGATATCCATCACCGCATCCAGTGCGcc                                                                                          <  1:834523/140‑1 (MQ=255)              ttATGCGGATAATGCTACCTCTGGTGAAGGAGTTGGCGAAGGCGTCTTGATGGGTTTGAAAATGGGAGCTGGGAGTTCTACCGCAGAGGCGGGGGAACTCCAAGTGATATCCATCACCGCATCCAGTGCGCCCGGTTTAt                                                                                  <  1:513194/140‑1 (MQ=255)                        aaTGCTACCTCTGGTTAAGGAGATGACGACGGCGTCTTGATGGGTTTGAAAATGGGAGCTGGGAGTTCTACCGCAGAGGCGGGGGAACTCCAAGTGATATCCATCACCGCATCCAGTGCGCCCGGTTTATCCCCGCTGAt                                                                        <  1:350459/140‑1 (MQ=255)                           gCTACCTCTGGTGAAGGAGTTGGCGAAGGCGTCTTGATGGGTTTGAAAATGGGAGCTGGGAGTTCTACCGCAGAGGCGGGGGGACTCCAAGTGATATCCAGCACGGCCACCAGTGCGACCCGCTTATCCCCGCTGATGCg                                                                     >  2:293801/1‑140 (MQ=255)                             tACCTCTGGTGAAGGAGTTGGCGAAGGCGTCTTGATGGGTTTGAAAATGGGAGCTGGGAGTTCTACCGCAGAGGCGGGGGAACTCCAAGTGATATCCATCACCGCATCCAGTGCGCCCGGTTTATCCCCGCTGATGCggg                                                                   <  1:631219/140‑1 (MQ=255)                               ccTCTGGTGAAGGAGTTGGCGAAGGCGTCTTGATGGGTTTGAAAATGGGAGCTGGGAGTTCTACCGCAGAGGCGGGGGAACTCCAAGTGATATCCATCACCGAATCCAGGGCGCACGGGTTATCACCGCTGATGCGGGGa                                                                 >  1:244956/1‑140 (MQ=255)                                     gTGAAGGAGTTGGCGAAGGCGTCTTGATGGGTTTGAAAATGGGAGCTGGGAGTTCTACCGCAGAGGCAGGGGAACTCCAAGTGATATCCATCACCGCATCCAGTGCGCCCGGTTTATCCCCGCTGATGCGGGGAACACCa                                                           >  2:488321/1‑140 (MQ=255)                                                         gTCTTGATGGGTTTGTAAATGGGAGCTGGGAGTTCTATCGCAGAGGCGGGGGAACTCCAAGTGATATCCATCACCGCATCCAGTGCGCCCGGTTTATCCCCGCTGATGCGGGGAACACCAGCGTCAGGCGTGAAATCTCa                                       <  1:325748/140‑1 (MQ=255)                                                                   gTTTGAAAATGGGAGCTGGGAGGTCCACCGCAGAGGCGGGGGAACTCCAAGTGATATCCATCACCGCATCCAGTGCGCCCGGTTTATCCCCGCTGATGCGGGGAACACCAGCGTCAGGCGTGAAATCTCACCGTCGTTGc                             <  1:357963/140‑1 (MQ=255)                                                                           aTGGGAGCTGGGAGTGCTACCGCAGTGGCAGGGGAACTCCAAGTGATATCCATCACCGCATCCAGTGCGCCCGGTTTATCCCCGCTGATGCGGGGAACACCAGCGTCAGGCGTGAAATCTCACCGTCGTTGCCGGTTTAt                     <  1:293801/140‑1 (MQ=255)                                                                              ggAGCTGGGAGTTCTACCGCAGAGGCGGGGGAACTCCAAGTGATATCCATCACCGCATCCAGTGCGCCCCGTTTATCCCCGCTGATGCGGGGGACACCAGCGTCCGGCCGGAAATCTCACCGGCGTTGCCGGTTTATccc                  >  2:357963/1‑140 (MQ=255)                                                                                     ggAGTTCTACCGCAGAGGCGGGGGAACTCCAAGGGATATCCATCACCCCATCCCGTGCGCCCGGGTTTTCCCCGCCGATGCCGGGAACACCAGCGGCAGGCGTGGAATCTCCCCCTCGTTGCCGGTTTATCCCTGCTGgc           >  2:128586/1‑140 (MQ=255)                                                                                      gAGTTCTACCGCAGAGGCGGGGCAACTCCAAGTGATATCCATCACCGCATCCAGTGCGCCCGGTTTATCCCCGCTGATGCGGGGAACACCAGCGTCAGGCGTGAAATCTCACCGTCGTTGCCGGTTTATCCCTGCTGgcg          <  1:325616/140‑1 (MQ=255)                                                                                              ccGCAGAGGCGGGGGCACTCCAAGTTATACCCATCACAGCATCCAGTGCGCCCGGTTTATCCCCGCTGATGCGGGGAACACCAGCGTCAGGCGTGAAATCTCACCGTCGTTGCCGGTTTATCCCTGCTGGCGCGGGGAAc  <  1:236414/140‑1 (MQ=255)                                                                                                                                  |                                                                                                        CGCTGCCGTGACGTTATGCGGATAATGCTACCTCTGGTGAAGGAGTTGGCGAAGGCGTCTTGATGGGTTTGAAAATGGGAGCTGGGAGTTCTACCGCAGAGGCGGGGGAACTCCAAGTGATATCCATCATCGCATCCAGTGCGCCCGGTTTATCCCCGCTGATGCGGGGAACACCAGCGTCAGGCGTGAAATCTCACCGTCGTTGCCGGTTTATCCCTGCTGGCGCGGGGAAC  >  NC_000913/2877556‑2877788

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑