Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,878,876	A→G	D299D (GAT→GAC)	cas1 ←	multifunctional nuclease Cas1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,878,876	0	A	G	100.0%	299.5 / NA	84	D299D (GAT→GAC)	cas1	multifunctional nuclease Cas1
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (44/40);  total (44/40)

TTTTGCGGATACATCACCTACATATACCCCTGCACGTACCTCCAACAACCAGATGGCTAATCTGCCTCGTAAGCGCGGAGGTACATTTTCAGTGACCACGACCAACATACTCATTTCAGCTACTCCGATGGCCTGCATCTCCCAGTGAAACAGGAAGCGGAATGGCAACAGGCTGTGCATCTTCAGGTGGGGCCGGCGGTTGTATTTCTCCAGCGGCAAGCACGTCCTCTATAAGCGGAATCAATTTGGCTAATGTTTTACTACTGCGAAAA  >  NC_000913/2878740‑2879011                                                                                                                                         |                                                                                                                                        ttttGCGGATACATCACCTACATATACCCCTGCACGTACCTCCAACAACCAGATGGCTAATCTGCCTCGTAAGCGCGGAGGTACATTTTCAGTGACCACGACCAACATACTCATTTCAGCTACTCCGATGGCCTGCGtct                                                                                                                                      <  1:138036/140‑1 (MQ=255)          tACATCACCTACATATACCCCTGCACGTACCTCCAACAACCAGATGGCTAATCTGCCTCGTAAGCGCGGAGGTACATTTTCAGTGACCACGACCAACATACTCATTTCAGCTACTCCGATGGCCTGCGTCTCCCAGTGaa                                                                                                                             >  2:606167/1‑140 (MQ=255)           aCATCACCTACATATACCCCTGCACGTACCTCCAACAACCAGATGGCTAATCTGCCTCGTAAGCGCGGAGGTACATTTTCAGTGACCACGACCAACATACTCATTTCAGCTACTCCGATGGCCTGCGTCTCCCAGTGaaa                                                                                                                            >  2:532668/1‑140 (MQ=255)            cATCACCTACATATACCCCTGCACGTACCTCCAACAACCAGATGGCTAATCTGCCTCGTAAGCGCGGAGGTACATTTTCAGTGACCACGACCAACATACTCATTTCAGCTACTCCGATGGCCTGCGTCTCCCAGTGAAAc                                                                                                                           <  2:102718/140‑1 (MQ=255)                     cATATACCCCTGCACATACCTCCAACAACCAGATGGCCAATCTGCCTCGTAAGCGCGGAGGTACATTTTCAGTGACCACGACCAACATACTCATTTCAGCTACTCCGATGGCCTGCGTCTCCCAGTGAAACAGGAAGCgg                                                                                                                  <  1:220213/140‑1 (MQ=255)                       tataCCCCTGCACGTACCTCCAACAACCAGATGGCTAATCTGCCTCGTAAGCGCGGAGGTACATTTTCAGTGACCACGACCAACATACTCATTTCAGCTACTCCGATGGCCTGCGTCTCCCAGTGAAACAGGAAGCGGaa                                                                                                                <  1:606167/140‑1 (MQ=255)                       tataCCCCTGCACGTACCTCCAACAACCAGATGGCTAATCTGCCTCGTAAGCGCGGAGGTACATTTTCAGTGACCACGACCAACATACTCATTTCAGCTACTCCGATGGCCTGCGTCTCCCAGTGAAACAGGAAGCGGaa                                                                                                                >  1:4429/1‑140 (MQ=255)                       tataCCCCTGCACGTACCTCCAACAACCAGATGGCTAATCTGCCTCGTAAGCGCGGAGGTACATTTTCAGTGACCACGACCAACATACTCATTTCAGCTACTCCGATGGCCTGCGTCTCCCAGTGAAACAGGAAGCGGaa                                                                                                                <  2:67203/140‑1 (MQ=255)                         taCCCCTGCACGTACCTCCAACAACCAGATGGCTAATCTGCCTCGTAAGCGCGGAGGTACATTTTCAGTGACCACGACCAACATACTCATTTCAGCTACTCCGATGGCCTGCGTCTCCCAGTGAAACAGGAAGCGGAATg                                                                                                              >  1:10244/1‑140 (MQ=255)                          aCCCCTGCACGTACCTCCAACAACCAGATGGCTAATCTGCCTCGTAAGCGCGGAGGTACATTTTCAGTGACCACGACCAACATACTCATTTCAGCTACTCCGCTGGCCTGCGTCTCCAAGTGAAACAGGAAGCGGAATgg                                                                                                             >  1:348077/1‑140 (MQ=255)                            cccTGCACGTACCTCCAACAACCAGATGGCTAATCTGCCTCGTAAGCGCGGAGGTACATTTTCAGTGACCACGACCAACATACTCATTTCAGCTACTCCGATGGCCTGCGTCTCCCAGTGAAACAGGAAGCGGaa                                                                                                                >  1:203208/1‑135 (MQ=255)                            cccTGCACGTACCTCCAACAACCAGATGGCTAATCTGCCTCGTAAGCGCGGAGGTACATTTTCAGTGACCACGACCAACATACTCATTTCAGCTACTCCGATGGCCTGCGTCTCCCAGTGAAACAGGAAGCGGaa                                                                                                                <  2:203208/135‑1 (MQ=255)                            cccTGCACGTACCTCCAACAACCAGATGGCTAATCTGCCTCGTAAGCGCGGAGGTACATTTTCAGTGACCACGACCAACATACTCATTTCAGCTACTCCGATGGCCTGCGTCTCCCAGTGAAACAGGAAGCGGAATGGCa                                                                                                           >  1:835673/1‑140 (MQ=255)                             ccTGCACGTACCTCCAACAACCAGATGGCTAATCTGCCTCGTAAGCGCGGAGGTACATTTTCAGTGACCACGACCAACATACTCATTTCAGCTACTCCGATGACCTGCGTGTCCGAGGGAAACAAGAAGCGGAATGGCaa                                                                                                          >  1:238576/1‑140 (MQ=255)                               tGCACGTACCTCCAACAACCAGATGGCTAATCTGCCTCGTAAGCGCGGAGGTACATTTTCAGTGACCACGACCAACATACTCATTTCAGCTACTCCGATGGCGTGCGTCTCCCATTGGAACAGGAAGCGGAATGGCAACa                                                                                                        >  1:275134/1‑140 (MQ=255)                               tGCACGTACCTCCAACAACCAGATGGCTAATCTGCCTCGTAAGCGCGGAGGTACATTTTCAGTGACCACGACCAACATACTCATTTCAGCTACTCCGATGGCGTGCGTCTACCAGTGCAACAGGAAGCGGAATGGCAACa                                                                                                        >  1:222044/1‑140 (MQ=255)                               tGCACGTACCTCCAACAACCAGATGGCTAATCTGCCTCGTAAGCGCGGAGGTACATTTTCAGTGACCACGACCAACATACTCATTTCAGCTACTCCGATGGCCTGCGTCTCCCAGTGAAACAGGAAGCGGAATGGCaa                                                                                                          <  2:115800/138‑1 (MQ=255)                               tGCACGTACCTCCAACAACCAGATGGCTAATCTGCCTCGTAAGCGCGGAGGTACATTTTCAGTGACCACGACCAACATACTCATTTCAGCTACTCCGATGGCCTGCGTCTCCCAGTGAAACAGGAAGCGGAATGGCaa                                                                                                          >  1:115800/1‑138 (MQ=255)                               tGCACGTACCTCCAACAACCAGATGGCTAATCTGCCTCGTAAGCGCGGAGGTACATTTTCAGTGACCACGACCAACATACTCATTTCAGCTACTCCGATGGCCTGCGTCTCCCAGTGAAACAGGAAGCGGAATGGCAACa                                                                                                        >  1:629004/1‑140 (MQ=255)                               tGCACGTACCTCCAACAACCAGATGGCTAATCTGCCTCGTAAGCGCGGAGGTACATTTTCAGTGACCACGACCAACATACTCATTTCAGCTACTCCGATGGCCTGCGTCTCCCAGTGAAACAGGAAGCGGAATGGCAACa                                                                                                        >  1:674858/1‑140 (MQ=255)                               tGCACGTACCTCCAACAACCAGATGGCTAATCTGCCTCGTAAGCGCGGAGGTACATTTTCAGTGACCACGACCAACATACTCATTTCAGCTACTCCGATGGCCTGCGTCTCCCAGTGAAACAGGAAGCGGAATGGCAACa                                                                                                        >  2:590820/1‑140 (MQ=255)                               tGCACGTACCTCCAACAACCAGATGGCTAATCTGCCTCGTAAGCGCGGAGGTACATTTTCAGTGACCACGACCAACATACTCATTTCAGCTACTCCGATGGCCTGCGTCTCCCAGTGAAACAGGAAGCGGAATGGCAACa                                                                                                        >  1:743996/1‑140 (MQ=255)                               tGCACGTACCTCCAACAACCAGATGGCTAATCTGCCTCGTAAGCGCGGAGGTACATTTTCAGTGACCACGACCAACATACTCATTTCAGCTACTCCGATGGCCTGCGTCTCCCAGTGAAACAGGAAGCGGAATGGCAACa                                                                                                        >  1:437282/1‑140 (MQ=255)                               tGCACGTACCTCCAACAACCAGATGGCTAATCTGCCTCGTAAGCGCGGAGGCACATTTTCAGTGACCACGACCAACATACTCATTTCAGCTACTCCGATGGCCTGCGTCTCCCAGTGAAACAGGAAGCGGAATGGCAACa                                                                                                        >  2:836125/1‑140 (MQ=255)                                gCACGTACCTCCAACAACCAGATGGCTAATCTGCCTCGTAAGCGCGGAGGTACATTTTCAGTGACCACGACCAACATACTCATTTCAGCTACTCCGATGGCCTGCGTCTCCCAGTGAAACAGGAAGCGGAATGGCAACAg                                                                                                       >  1:12743/1‑140 (MQ=255)                                gCACGTACCTCCAACAACCAGATGGCTAATCTGCCTCGTAAGCGCGGAGGTACATTTTCAGTGACCACGACCAACATACTCATTTCAGCTACTCCGATGGCCTGCGTCTCCCAGTGAAACAGGAAGCGGAATGGCAACAg                                                                                                       >  2:707279/1‑140 (MQ=255)                                 cACGTACCTCCAACAACCAGATGGCTAATCTGCCTCGTAAGCGCGGAGGTACATTTTCAGTGACCACGACCAACATACTCATTTCAGCTACTCCGATGGCCTGCGTCTCCCAGTGAAACAGGAAGCGGAATGGCAACAgg                                                                                                      >  2:493147/1‑140 (MQ=255)                                 cACGTACCTCCAACAACCAGATGGCTAATCTGCCTCGTAAGCGCGGAGGTACATTTTCAGTGACCACGACCAACATACTCATTTCAGCTACTCCGATGGCCTGCGTCTCCCAGTGAAACAGGAAGCGGAATGGCAACAgg                                                                                                      >  1:114523/1‑140 (MQ=255)                                     tACCTCCAACAACCAGACGGCTTATCTGCCTCGTACGCGCGGAGGTACCTTTTCAGTGACCACGACCAACATACTCATTTCAGCTACTCCGATGGCCTGCGTCTCCCAGTGAAACAGGAAGCGGAATGGCAACAGGCtgt                                                                                                  <  2:238576/140‑1 (MQ=255)                                      aCCTCCAACAACCAGTTGGCTAATCGGCCCCGTAAGCGCGGAGGTACATTTTCAGTGACCACGACCAACATACTCATTTCAGCTACTCCGATGGCCTGCGTCTCCCAGTGAAACAGGAAGCGGAATGGCAACAGGCtgtg                                                                                                 <  1:314480/140‑1 (MQ=255)                                      aCCTCCAACAACCAGATGGCTAATCTGCCTCGTAAGCGCGGAGGTACATTTTCAGTGACCACGACCAACATACTCATTTCAGCTACTCCGATGGCCTGCGTCTCCCAGTGAAACAGGAAGCGGAATGGCAACAGGCtgtg                                                                                                 >  1:749761/1‑140 (MQ=255)                                          ccAACAACCAGATGGCTAATCTGCCTCGTAAGCGCGGAGGTACATTTTCAGTGACCACGACCAACATACTCATTTCAGCTACTCCGATGGCCTGCGTCTCCCAGTGAAACAGGAAGCGGAATGGCAACAGGCTGTGCATc                                                                                             <  2:10244/140‑1 (MQ=255)                                              caacCAGATGGCTAATCTGCCTCGTAAGCGCGGAGGTACATTTTCAGTGACCACGACCAACATACTCATTTCAGCTACTCCGATGGCCTGCGTCTCCCAGTGAAACAGGAAGCGGAATGGCAACAGGCtgtg                                                                                                 >  2:472468/1‑132 (MQ=255)                                              caacCAGATGGCTAATCTGCCTCGTAAGCGCGGAGGTACATTTTCAGTGACCACGACCAACATACTCATTTCAGCTACTCCGATGGCCTGCGTCTCCCAGTGAAACAGGAAGCGGAATGGCAACAGGCtgtg                                                                                                 <  1:472468/132‑1 (MQ=255)                                                     aTGGCTAATCTGCCTCGTAAGCGCGGAGGTACATTTTCAGTGACCACGACCAACATACTCATTTCAGCTACTCCGATGGCCTGCGTCTCCCTGTGAAACAAGAATCGGTATGGCAACCGGCTGTGCATCTTCAGGTgggg                                                                                  >  2:276437/1‑140 (MQ=255)                                                     aTGGCTAATCTGCCTCGTAAGCGCGGAGGTACATTTTCAGTGACCACGACCAACATACTCATTTCAGCTACTCCGATGGCCTGCGTCTCCCAGTGAAACAGGAAGCGGAATGGCAACAGGCTGTGCATCTTCAGGTgggg                                                                                  >  2:510572/1‑140 (MQ=255)                                                         cTAATCTGCCTCGTAAGCGCGGAGGTACATTTTCAGTGACCACGACCAACATACTCATTTCAGCTACTCCGATGGCCTGCGTCTCCCAGTGAAACAGGAAGCGGAATGGCAACAGGCTGTGCATCTTCAGGTGGGGCcgg                                                                              <  2:462159/140‑1 (MQ=255)                                                             tCTGCCTCGTAAGCGCGGAGGTCCATTTTCACTGACCACTACCAACATACTCATTTCAGCTACTCCGATGGCCTGCGTCTCCCAGTGAAACAGGAAGCGGAATGGCAACAGGCTGTGCATCTTCAGGTGGGGCCGGCGGt                                                                          <  2:275134/140‑1 (MQ=255)                                                             tCTGCCTCGTAAGCGCGGAGGTACATTTTCAGTGACCACGACCAACATACTCATTTCAGCTACTCCGATGGCCTGCGTCTCCCAGTGAAACAGGAAGCGGAATGGCAACAGGCTGTGCATCTTCAGGTGGGGCCGGCGGt                                                                          <  2:749761/140‑1 (MQ=255)                                                                gCCTCGTAAGCGCGGAGGTACATTTTCAGTGACCACGACCAACATACTCATTTCAGCTACTCCGATGGCCTGCGTCTCCCAGTGAAACAGGAAGCGGAATGGCAACAGGCTGTGCATCTTCAGGTGGGGCcggcgg                                                                           >  2:19379/1‑136 (MQ=255)                                                                gCCTCGTAAGCGCGGAGGTACATTTTCAGTGACCACGACCAACATACTCATTTCAGCTACTCCGATGGCCTGCGTCTCCCAGTGAAACAGGAAGCGGAATGGCAACAGGCTGTGCATCTTCAGGTGGGGCcggcgg                                                                           <  1:19379/136‑1 (MQ=255)                                                                  cTCGTAAGCGCGGAGGTACATTTTCAGTGACCACGACCAACATACTCATTTCAGCTACTCCGATGGCCTGCGTCTCCCAGTGAAACAGGAAGCGGAATGGCAACAGGCTGTGCATCTTCAGGTGGGGCCGGCGGTTGTAt                                                                     >  1:778596/1‑140 (MQ=255)                                                                      tAAGCGCGGAGGTACATTTTCAGTGACCACGACCAACATACTCATTTCAGCTACTCCGATGGCCTGCGTCTCCCAGTGAAACAGGAAGCGGAATGGCAACAGGCTGTGCAGCTTTAGATGGGGCAGGCGGTTGGAttttt                                                                 >  1:390429/1‑138 (MQ=255)                                                                           gcgGAGGTACATTTTCAGTGACCACGACCAACATACTCATTTCAGCTACTCCGATGGCCTGCGTCTCCCAGTGAAACAGGAAGCGGAATGGCAACAGGCTGTGCATCTTCAGGTGGGGCCGGCGGTTGTATTTCTCCAGc                                                            >  2:84894/1‑140 (MQ=255)                                                                               aGGTACATTTTCAGTGACCACGACCAACATACTCATTTCAGCTACTCCGATGGCCTGCGTCTCCCAGTGAAACAGGAAGCGGAATGGCAACAGGCTGTGCATCTTCAGGTGGGGCCGGCGGTTGTATTTCTCCAGCGGCa                                                        <  1:510572/140‑1 (MQ=255)                                                                               aGGTACATTTTCAGTGACCACGACCAACATACTCATTTCAGCTACTCCGATGGCCTGCGTCTCCCAGTGAAACAGGAAGCGGAATGGCAACAGGCTGTGCATCTTCAGGTGGGGCCGGCGGTTGTATTTCTCCAGCGGCa                                                        <  2:437282/140‑1 (MQ=255)                                                                               aGGTACATTTTCAGTGACCACGACCAACATACTCATTTCAGCTACTCCGATGGCCTGCGTCTCCCAGTGAAACAGGAAGCGGAATGGCAACAGGCTGTGCATCTTCAGGTGGGGCCGGCGGTTGTATTTCTCCAGCGGCa                                                        <  1:707279/140‑1 (MQ=255)                                                                                  tACATTTTCAGTGACCACGACCAACATACTCATTTCAGCTACTCCGATGGCCTGCGTCTCCCAGTGAAACAGGAAGCGGAATGGCAACAGGCTGTGCATCTTCAGGTGGGGCCGGCGGTTGTATTTCTCCAGCGGCAAGc                                                     <  1:107479/140‑1 (MQ=255)                                                                                  tACATTTTCAGTGACCACGACCAACATACTCATTTCAGCTACTCCGATGGCCTGCGTCTCCCAGTGAAACAGGAAGCGGAATGGCAACAGGCTGTGCATCTTCAGGTGGGGCCGGCGGTTGTATTTCTCCAGCGGCAAGc                                                     <  2:674858/140‑1 (MQ=255)                                                                                  tACATTTTCAGTGACCACGACCAACATACTCATTTCAGCTACTCCGATGGCCTGCGTCTCCCAGTGAAACAGGAAGCGGAATGGCAACAGGCTGTGCATCTTCAGGTGGGGCCGGCGGTTGTATTTCTCCAGCGGCAAGc                                                     <  1:532668/140‑1 (MQ=255)                                                                                  tACACTTTCAGTGACCACGAACAACATCCTCATTTCAGCTGCTCCCATGGCCTGCATCTCCCAGTGAAACAGGAAGCCGAATGGCAACAGGCTGTGCATCTTCAGGTGGGGCTGGCGGTTGTATTTCTCCAGCGGCAAGc                                                     <  2:114523/140‑1 (MQ=255)                                                                                     aTTTTCAGTGACCACGACCAACTTACTCATTGCAGATACTCCGATGGCCTGCGTCTCCCAGTGAAACAGGAAGCGGAATGGCAACAGGCTGTGCATCTTCAGGTGGGGCCGGCGGTTGTATTTCTCCAGCGGCAAGCACg                                                  <  2:273221/140‑1 (MQ=255)                                                                                      ttttCAGTGACCCCGACCAACATCCTCATTTCAGCTACTCCGATGGCCTGCGTCTCCCAGTGAAACAGGAAGCGGAATGGCAACAGGCTGTGCATCTTCAGGTGGGGCCGGCGGTTGTATTTCTCCAGCGGCAAGCACGt                                                 <  1:176831/140‑1 (MQ=255)                                                                                      ttttCAGTGACCACGACCAACATACTCATTTCAGCTACTCCGATGGCCTGCGTCTCCCAGTGAAACAGGAAGCGGAATGGCAACAGGCTGTGCATCTTCAGGTGGGGCCGGCGGTTGTATTTCTCCAGCGGCAAGCACGt                                                 <  2:12743/140‑1 (MQ=255)                                                                                      ttttCAGTGACCACGACCAACACTCTCATTTGAGCCACTGCGATGGCCTGCGTCTCCCAGTGAAACAGGAAGCGGAATGGCAACAGGCTGTGCATCTTCAGGTGGGGCCGGCGGTTGTATTTCTCCAGCGGCAAGCACGt                                                 <  2:348077/140‑1 (MQ=255)                                                                                        ttCAGTGACCACGACCAACATACTCATTTCAGCTACTCCGATGGCCTGCGTCTCCCAGTGAAACAGGAAGCGGAATGGCAACAGGCTGTGCATCTTCAGGTGGGGCCGGCGGTTGTATTTCTCCAGCGGCAAGCACGTcc                                               <  2:677094/140‑1 (MQ=255)                                                                                         tCAGTGACCACGACCAACATACTCATTTCAGCTACTCCGATGGCCTGCGTCTCCCAGTGAAACAGGAAGCGGAATGGCAACAGGCTGTGCATCTTCAGGTGGGGCCGGCGGTTGTATTTCTCCAGCGGCAAGCACGt                                                 >  1:742953/1‑137 (MQ=255)                                                                                         tCAGTGACCACGACCAACATACTCATTTCAGCTACTCCGATGGCCTGCGTCTCCCAGTGAAACAGGAAGCGGAATGGCAACAGGCTGTGCATCTTCAGGTGGGGCCGGCGGTTGTATTTCTCCAGCGGCAAGCACGt                                                 <  2:742953/137‑1 (MQ=255)                                                                                           aGTGACCACGACCAACATACTCATTTCAGCTACTCCGATGGCCTGCGTCTCCCAGTGAAACAGGAAGCGGAATGGCAACAGGCTGTGCATCTTCAGGTGGGGCCGGCGGTTGTATTTCTCCCGCGGCAAGCACGTCctct                                            >  1:649293/1‑140 (MQ=255)                                                                                           aGTGACCACGACCAACATACTCATTTCAGCTACTCCGATGGCCTGCGTCTCCCAGTGAAACAGGAAGCGGAATGGCAACAGGCTGTGCATCTTCAGGTGGGGCCGGCGGCTGTTTTTTTTCCGCGGGGAACACGGCctct                                            >  2:706104/1‑140 (MQ=255)                                                                                               aCCACGACCAACATACTCATTTCAGCTACTCCGATGGCCTGCGTCTCCCAGTGAAACAGGAAGCGGAATGGCAGCAGGCTGTGCATCTTCAGGTGGGGCCGGCGGTTGTTTTTTTCCCGCGGCAAGCACGTCCTCTATaa                                        >  2:559794/1‑140 (MQ=255)                                                                                               aCCACGACCAACATACTCATTTCAGCTACTCCGATGGCCTGCGTCTCCCAGTGAAACAGGAAGCGGAATGGCAACAGGCTGTGCATCTTCAGGTGGGGCCGGTGGTTGTAATTTACCCGCGGCATGCACGTCCTCTATaa                                        >  1:361977/1‑140 (MQ=255)                                                                                               aCCACGACCAACATACTCATTTCAGCTACTCCGATGGCCTGCGTCTCCCAGTGAAACAGGAAGCGGAATGGCAACAGGCTGTGCATCTTCAGGTGGGGCCCGCGGTTGGTTTTTTCCGGCGGGAAGCACGTCCTCTATaa                                        >  2:268689/1‑140 (MQ=255)                                                                                                ccACGACCAACATACTCATTTCAGCTACTCCGATGGCCTGCGTCTCCCAGTGAAACAGGAAGCGGAATGGCAACAGGCTGTGCATCTTCAGGTGGGGCCGGCGGTTGTATTTCTCCAGCGGCAAGCACGTCCTCTATAAg                                       <  2:629004/140‑1 (MQ=255)                                                                                                 cACGACCAACATACTCATTTCAGCTACTCCGATGGCCTGCGTCTCCCAGTGAAACAGGAAGCGGAATGGCAACAGGCTGTGCATCTTCAGGTGGGGCCGGCGGTTGTATTTCTCCAGCGGCAAGCACGTCCTCTATAAGc                                      >  1:497624/1‑140 (MQ=255)                                                                                                   cGACCAACATACTCATTTCAGCTACTCCGATGGCCTGCGTCTCCCAGTGAAACAGGAAGCGGAATGGCAACAGGCTGTGCATCTTCAGGTGGGGCCGGCGGTTGTATTTCTCCAGCGGCAAGCACGTCCTCTATAAGCgg                                    >  1:602137/1‑140 (MQ=255)                                                                                                         aCATACTCATTTCAGCTACTCCGATGGCCTGCGTCTCCCAGTGAAACAGGAAGCGGAATGGCAACAGGCTGTGCATCTTCAGGTGGGGCCGGCGGTTGTATTTCTCCAGCGGCAAGCACGTCCTCTATAAGCGGAATCaa                              <  2:743996/140‑1 (MQ=255)                                                                                                           aTACTCATTTCAGCTACTCCGATGGCCTGCGTCTCCCAGTGAAACAGGAAGCGGAATGGCAACAGGCTGTGCATCTTCAGGTGGGGCCGGCGGTTGTATTTCTCCAGCGGCAAGCACGTCCTCTATAAGCGGAATCAAtt                            <  1:84894/140‑1 (MQ=255)                                                                                                               tCATTTCAGCTACTCCGATGGCTTGCGTCCCCCAGTGGAACAGGAAGCGGAATGGCAACAGGCTGTGCATCTTCAGGTGGGGCCGGCGGTTGTATTTCTCCAGCGGCAAGCACGTCCTCTATAAGCGGAATCAATTTGGc                        <  1:268689/140‑1 (MQ=255)                                                                                                               tCATTTCAGCTACTCCGATGGCCTGCGTCTCCCAGTGAAACAGGAAGCGGAATGGCAGCAGGCTGTGCATCTTCAGGTGGGGCCGGCGGTTGTATTTCTCCAGCGGCAAGCACGTCCTCTATAAGCGGAATCAATTTGGc                        <  1:559794/140‑1 (MQ=255)                                                                                                               tCATTTCAGCTACTCCGATGGCCTGCGTCTCCCAGTGAAACAGGAAGCGGAATGGCAACAGGCTGTGCATCTTCAGGTGGGGCCGGCGGTTGTATTTCTCCAGCGGCAAGCACGTCCTCTATAAGCGGAATCAATTTGGc                        <  1:706104/140‑1 (MQ=255)                                                                                                               tCATTTCAGCTACTCCGATGGCCTGCGTCTCCCAGTGAAACAGGAAGCGGAATGGCAACAGGCTGTGCATCTTCAGGTGGGGCCGGCGGTTGTATTTCTCCAGCGGCAAGCACGTCCTCTATAAGCGGAATCAATTTGGc                        <  1:716141/140‑1 (MQ=255)                                                                                                                cATTTCAGCTACTCCGTGGGCCGGCGTCTCCCAGTCAAACAGGAAGCGAAATGGCAACAGGCTGTGCATCTTCAGGTGGGGCCGGCGGTTGTATTTCTCCAGCGGCAAGCACGTCCTCTATAAGCGGAATCAATTTGGCt                       <  2:390429/140‑1 (MQ=255)                                                                                                                cATTTCAGCTACCCCGATGGCCAGCGTCTCCAAGTGAAACAGGAAGCGGAATGGCAACAGGCTGTGCATCTTCAGGTGGGGCCGGCGGTTGTATTTCTCCAGCGGCAAGCACGTCCTCTATAAGCGGAATCAATTTGGCt                       <  2:361977/140‑1 (MQ=255)                                                                                                                 aTTTCAGCTACTCCGATGGCCTGCGTCTCCCAGTGAAACAGGAAGCGGAATGGCAACAGGCTGTGCATCTTCAGGTGGGGCCGGCGGTTGTATTTCTCCAGCGGCAAGCACGTCCTCTATAAGCGGAATCAATTTGGCTa                      <  2:154032/140‑1 (MQ=255)                                                                                                                  tttCAGCTACTCCGATGTCCTGCGTCTCCCACTGATACACGAAGCGGACTGGCAACAGGCTGTGCATCTTCAGGTGGGGCCGGCGGTTGTATTTCTCCAGCGGCAAGCACGTCCTCTATAAGCGGAATCAATTTGGCTaa                     <  2:222044/140‑1 (MQ=255)                                                                                                                     cAGCTACTCCGATGGCCTGCGTCTCCCAGTGAAACAGGAAGCGGAATGGCAACAGGCTGTGCATCTTCAGGTGGGGCCGGCGGTTGTATTTCTCCAGCGGCAAAACCGGCCTCTTTAAGCGGAAACAATTTTGCTAAttt                  >  1:530073/1‑138 (MQ=255)                                                                                                                       gCTACTCCGATGGCCTGCGTCTCCCAGTGAAACAGGAAGCGGAATGGCAACAGGCTGTGCATCTTCAGGTGGGGCCGGCGGTTGTATTTCTGCAGCGGCAAACAGGTCATCTATAAGAGGAAGCAATTTGGCTAATGttt                >  2:243934/1‑140 (MQ=255)                                                                                                                         tACTCCGATGGCCTGCGTCTCCCAGTGAAACAGGAAGCGGAATGGCAACAGGCTGTGCATCTTCAGGTGGGGCCGGCGGTTGTATTTCTCCAGCGGCAAGCACGTCCTCTATAAGCGGAATCAATTTGGCTAATGTTTta              >  1:47188/1‑140 (MQ=255)                                                                                                                           cTCCGATGGCCTGCGTCTCCCAGTGAAACAGGAAGCGGAATGGCAACAGGCTGTGCATCTTCAGGTGGGGCCGGCGGTTGTATTTCTCCAGCGGCAAGCACGTCCTCTATAAGCGGAATCAATTTGGCTAATGTTTtact            >  1:513713/1‑140 (MQ=255)                                                                                                                            tCCGATGGCCTGCGTCTCCCAGTGAAACAGGAAGCGGAATGGCAACAGGCTGTGCATCTTCAGGTGGGGCCGGCGGTTGTATTTCTCCAGCGGCAAGCACGTCCTCTATAAGCGGAATCAATTTGGCTAATGTTTtacta           <  2:778596/140‑1 (MQ=255)                                                                                                                               gATGGCCTGCGTCTCCCAGTGAAACAGGAAGCGGAATGGCAACAGGCTGTGCATCTTCAGGTGGGGCCGGCGGTTGTATTTCTCCAGCGGCAAGCACGTCCTCTATAAGCGGAATCAATTTGGCTAATGTTTTACTACTg        <  2:47188/140‑1 (MQ=255)                                                                                                                                aTGGCCTGCGTCTCCCAGTGAAACAGGAAGCGGAATGGCAACAGGCTGTGCATCTTCAGGTGGGGCCGGCGGTTGTTTTTCCCCAGCCGCCAGGCCGTCCTCCATAAGAGGGAACAATTTGGCTAATGTTTTtttactta       >  2:157094/1‑138 (MQ=255)                                                                                                                                aTGGCCTGCGTCTCCCAGTGAAACAGGAAGCGGAATGGCAACAGGCTGTGCATCTTCAGGTGGGGCCGGCGGTTGTATTTTTCCAGCGGCCAGCACGTCCCCTACAAAAGGAATCAATTTTGATAATGTTTTATTACTGc       >  2:370942/1‑140 (MQ=255)                                                                                                                                 tGGCCTGCGTCTCCCAGTGAAACAGGAAGCGGAATGGCAACAGGCTGTGCATCTTCAGGTGGGGCCGGCGGTTGTATTTCTCCAGCGGCAAGCACGTCCTCTATAAGCGGAATCAATTTGGCTAATGTTTTACTACTGCg      <  2:4429/140‑1 (MQ=255)                                                                                                                                 tGGCCTGCGTCTCCCAGTGAAACAGGAAGCGGAATGGCAACAGGCTGTGCATCTTCAGGTGGGGCCGGCGGTTGTATTTCTCCAGCGGCAAGCACGTCCTCTATAAGCGGAATCAATTTGGCTAATGTTTTACTACTGCg      <  1:157094/140‑1 (MQ=255)                                                                                                                                     cTGCGTCTCCCAGTGAAACAGGAAGCGGAAGGGCAACAGGCTGTGCATCTTCAGGTGGGGCCGGCGGTTGTATTTCTCCAGCGGCAAGCACGTCCTCTATAAACGGAATCAAATTTGCTAATGTTTTTCTACTGCGaaaa  >  2:536155/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                        TTTTGCGGATACATCACCTACATATACCCCTGCACGTACCTCCAACAACCAGATGGCTAATCTGCCTCGTAAGCGCGGAGGTACATTTTCAGTGACCACGACCAACATACTCATTTCAGCTACTCCGATGGCCTGCATCTCCCAGTGAAACAGGAAGCGGAATGGCAACAGGCTGTGCATCTTCAGGTGGGGCCGGCGGTTGTATTTCTCCAGCGGCAAGCACGTCCTCTATAAGCGGAATCAATTTGGCTAATGTTTTACTACTGCGAAAA  >  NC_000913/2878740‑2879011

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑