| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,892,952 | T→C | V99A (GTA→GCA) | ygcN → | putative oxidoreductase with FAD/NAD(P)‑binding domain |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,892,952 | 0 | T | C | 98.0% | 169.9 / NA | 50 | V99A (GTA→GCA) | ygcN | putative oxidoreductase with FAD/NAD(P)‑binding domain |
| Reads supporting (aligned to +/- strand): ref base T (1/0); new base C (23/26); total (24/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.80e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CGCGGAACTCCTCCCGCAATTTCATCTGACCGCGCCTCTTGAACGACGCATCACTCACGAAAGCCTTTCCCTGTTAACGCCCGATGGCGTAACGACGTTTTCCAGCTTACAGCCCGGCGGTGAATCCTGGAGTGTATTACGTGCACGATTCGATCCGTGGCTGGTTGCCGAAGCCGAAAAAGAAGGTGTCGAATGCATCCCCGGAGCGACGGTGGATGCACTGTATGAAGAAAACGGCAGAGTCTGTGGCGTTATTTGTGGTGACGA > NC_000913/2892818‑2893084 | cgcgGAACTCCTCCCGCAATTTCATCTGACCGCGCCTCTTGAACGACGCATCACTCACGAAAGCCTTTCCCTGTTAACGCCCGATGGCGTAACGACGTTTTCCAGCTTACAGCCCGGCGGTGAATCCTGGAGTGCATTAc > 1:476267/1‑140 (MQ=255) gcgGAACTCCTCCCGCAATTTCATCTGACCGCGCCTCTTGAACGACGCATCACTCACGAAAGCCTTTCCCTGTTAACGCCCGATGGCGTAACGACGTTTTCCAGCTTACAGCCCGGCGGTGAATCCTGGAGTGCATTACg > 2:476669/1‑140 (MQ=255) ggAACTCCTCCCGCAATTTCATCTGACCGCGCCTCTTGAACGACGCATCACTCACGAAAGCCTTTCCCTGTTAACGCCCGATGGCGTAACGACGTTTTCCAGCTTACAGCCCGGCGGTGAATCCTGGAGTGCATTACGTg < 2:451077/140‑1 (MQ=255) gAACTCCTCCCGCAATTTCATCTGACCGCGCCTCTTGAACGACGCATCACTCACGAAAGCCTTTCCCTGTTAACGCCCGATGGCGTAACGACGTTTTCCAGCTTACAGCCCGGCGGTGAATCCTGGAGTGCATTACGTGc < 1:817345/140‑1 (MQ=255) ccGCAATTTCATCTGCCCGCGCTTCGTGAACGACGCAGCACTCACGAAAGCCTTTCCCTGTTAACGCCCGATGGCGTAACGACGTTTTCCAGCTTACAGCCCGGCGGTGAATCCTGGAGTGCATTACGTGCACGATTCGa < 1:331940/140‑1 (MQ=255) ttCATCTGACCGCGCCTCTTGAACGACGCATCACTCACGAAAGCCTTTCCCTGTTAACGCCCGATGGCGTAACGACGTTTTCCAGCTTACAGCCCGGCGGTGGATCCTGGAGTGTATAACGTGCACGATTCGATCCGTgg > 1:233912/1‑140 (MQ=255) cATCTGACCGCGCCTCTTGAACGACGCATCACTCACGAAAGCCTTTCCCTGTTAACGCCCGATGGCGTAACGACGTTTTCCAGCTTACAGCCCGGCGGTGAATCCTGGAGTGCATTACGTGCACGATTCGATCCGTGGCt > 2:523234/1‑140 (MQ=255) cATCTGACCGCGCCTCTTGAACGACGCATCACTCACGAAAGCCTTTCCCTGTTAACGCCCGATGGCGTAACGACGTTTTCCAGCTTACAGCCCGGCGGTGAATCCTGGAGTGCATTACGTGCACGATTCGATCCGTGGCt > 2:622676/1‑140 (MQ=255) ccGCGCCTCTTGAACGACGCATCACTCACGAAAGCCTTTCCCTGTTAACGCCCGATGGCGTAACGACGTTTTCCAGCTTACAGCCCGGCGGTGAATCCTGGAGTGCATTACGTGCACGATTCGATCCGTGGCTGGTTgcc > 2:634656/1‑140 (MQ=255) ccGCGCCTCTTGAACGACGCATCACTCACGAAAGCCTTTCCCTGTTAACGCCCGATGGCGTAACGACGTTTTCCAGCTTACAGCCCGGCGGTGAATCCTGGAGTGCATTACGTGCACGATTCGATCCGTGGCTGGTTgcc > 2:778526/1‑140 (MQ=255) ccGCGCCTCTTGAACGACGCATCACTCACGAAAGCCTTTCCCTGTTAACGCCCGATGGCGTAACGACGTTTTCCAGCTTACAGCCCGGCGGAGAATCCTGCAGTACATTACGTGCACCATTCCATCCGTGGCTGGTTgcc > 2:299076/1‑140 (MQ=255) ctctTGAACGACGCATCACTCACGAAAGCCTTTCCCTGTTAACGCCCGATGGCGTAACGACGTTTTCCAGCTTACAGCCCGGCGGTGAATCCTGGAGTGCATTACGTGCACGATTCGATCCGTGGCTGGTTgccgaagcc < 1:634656/140‑1 (MQ=255) ttGAACGACGCATCACTCACGAAAGCCTTTCCCTGTTAACGCCCGATGGCGTAACGACGTTTTCCAGCTTACAGCCCGGCGGTGAATCCTGGAGTGCATTACGTGCACGATTCGATCGGTGGCTGGTTgccgaagccgaa > 1:318085/1‑140 (MQ=255) ttGAACGACGCATCACTCACGAAAGCCTTTCCCTGTTAACGCCCGATGGCGTAACGACGTTTTCCAGCTTACAGCCCGGCGGTGAATCCTGGAGTGCATTACGTGCACGATTCGATCCGTGGCTGGTTgccgaagccgaa < 1:523234/140‑1 (MQ=255) ttGAACGACGCATCACTCACGAAAGCCTTTCCCTGTTAACGCCCGATGGCGTAACGACGTTTTCCAGCTTACAGCCCGGCGGTGAATCCTGGAGTGCATTACGTGCACGATTCGATCCGTGGCTGGTTgccgaagccgaa > 1:733912/1‑140 (MQ=255) acgacgCATCACTCACGAAAGCCTTTCCCTGTTAACGCCCGATGGCGTAACGACGTTTTCCAGCTTACAGCCCGGCGGTGAATCCTGGAGTGCATTACGTGCACGATTCGATCCGTGGCTGGTTGCCGAAGCCGAAAaag < 2:476267/140‑1 (MQ=255) acgCATCACTCACGAAAGCCTTTCCCTGTTAACGCCCGATGGCGTAACGACGTTTTCCAGCTTACAGCCCGGCGGTGAATCCTGGAGTGCATTACGTGCACGATTCGATCCGTGGCTGGTTGCCGAAGCCGAAAaagaag < 1:778526/140‑1 (MQ=255) acgCATCACTCACGAAAACCTTACCCTGTTATCGCCCGATGGCGTAACAACGTTTTCCAGCTTACAGCCCGGCGGTGAATCCTGGAGTGCATTACGTGCACGATTCGATCCGTGGCTGGTTGCCGAAGCCGAAAaagaag < 2:233912/140‑1 (MQ=255) cgCATCACTCACGAAAGCCTTTCCCTGTTAACGCCCGATGGCGTAACGACGTTTTCCAGCTTACAGCCCGGCGGTGAATCCTGGAGTGCATTACGTGCACGATTCGATCCGTGGCTGGTTGCCGAAGCCGAAAAAGAAgg > 2:788073/1‑140 (MQ=255) tcactcacGAAAGCCTTTCCCTCTTTACGGCCGATGGCGTAACGACGTTTTCCAGCTTACAGCCCGGCGGTGAATCCTGGAGTGCATTACGTGCACGATTCGATCCGTGGCTGGTTGCCGAAGCCGAAAAAGAAGGTGTc < 1:251500/140‑1 (MQ=255) cacGAAAGCCTTTCCCTGTTAACGCCCGATGGCGTAACGACGTTTTCCAGCTTACAGCCCGGCGGTGAATCCTGGAGTGCATTACGTGCACGATTCGATCCGTGGCTGGTTGCCGAAGCCGAAAAAGAAGGTGTCGAATg < 2:733912/140‑1 (MQ=255) cacGAAAGCCTTTCCCTATTAAAGCCCGATGTCGTAACGCCGTTTTCCCGCTTACAGCCCGGCGGTGAATCCTGGAGTGCATTACGTGCACGATTCGATCCGTGGCTGGTTGCCGAAGCCGAAAAAGAAGGTGTCGAATg < 2:318085/140‑1 (MQ=255) acGAAAGCCTTTCCCTGTTAACGCCCGATGGCGTAACGACGTTTTCCAGCTTACAGCCCGGCGGTGAATCCTGGAGTGCATTACGTGCACGATTCGATCCGTGGCTGGTTGCCGAAGCCGAAAAAGAAGGTGTCGAATGc > 2:791527/1‑140 (MQ=255) ttCCCTGTTAACGCCCGATGGCGTAACGACGTTTTCCAGCTTACAGCCCGGCGGTGAATCATGGAGTGCATTACGTGCACGATTCGATCCGTGGCTGGTTGCCGAAGCCGAAAAAGAAGGTGTCGAATGCATCCCCGGAg > 1:178976/1‑140 (MQ=255) cccTGTTAACGCCCGATGGCGTAACGACGTTTTCCAGCTTACAGCCCGGCGGTGAATCCTGGAGTGCATTACGTGCACGATTCGATCCGTGGCTGGTTGCCGAAGCCGAAAAAGAAGGTGTCGAATGCATCCCCGGAGCg > 2:148454/1‑140 (MQ=255) ccTGTTAACGCCCGATGGCGTAACGACGTTTTCCAGCTTACAGCCCGGCGGTGAATCCTGGAGTGCATTACGTGCACGATTCGATCCGTGGCTGGTTGCCGAAGCCGAAAAAGAAGGTGTCGAATGCATCCCCGGAGCGa < 2:458113/140‑1 (MQ=255) gtGTTAACGCCCGATGGCGTAAAGAGGTTTTCCAGCTGACAGCCCGGCGGTGAATCCTGGAGTGCATTACGTGCACGATTCGATCCGTGGCTGGTTGCCGAAGCCGAAAAAGAAGGTGTCGAATGCATCCCCGGAGCGAc < 1:354063/139‑1 (MQ=255) cTGTTAACGCCCGATGGCGTAACGACGTTTTCCAGCTTACAGCCCGGCGGTGAATCCTGGAGTGCATTACGTGCACGATTCGATCCGTGGCTGGTTGCCGAAGCCGAAAAAGAAGGTGTCGAATGCATCCCCGGAGCGAc < 1:476669/140‑1 (MQ=255) ttAACGCCCGATGGCGTAACGACGTTTTCCAGCTTACAGCCCGGCGGTGAATCCTGGAGTGCATTACGTGCACGATTCGATCCGTGGCTGGTTGCCGAAGCCGAAAAAGAAGGTGTCGAATGCATCCCCGGAGCGACGGt > 2:459945/1‑140 (MQ=255) tAACGCCCGATGGCGTAACGACGTTTTCCAGCTTACAGCCCGGCGGTGAATCCTGGAGTGCATTACGTGCACGATTCGATCCGTGGCTGGTTGCCGAAGCCGAAAAAGAAGGTGTCGAATGCATCCCCGGAGCGACGGTg < 1:677067/140‑1 (MQ=255) aaCGCCCGATGGCGTAACGACGTTTTCCAGCTTACAGCCCGGCGGTGAATCCTGGAGTGCATTACGTGCACGATTCGATCCGTGGCTGGTTGCCGAAGCCGAAAAAGAAGGTGTCGAATGCATCCCCGGAGCGACGGTgg < 1:459945/140‑1 (MQ=255) aCGGCCGATGGCGTAACTACGTTTTCCAGCTCACGCCCCTGCGGTGAACCCTGGAGTGCATTACGTGCACGATTCGATCCGTGGCTGGTTGCCGAAGCCGAAAAAGAAGGTGTCGAATGCATCCCCGGAGCGACGGTGGa < 2:219324/140‑1 (MQ=255) aCGCCCGATGGCGTAACGACGTTTTCCAGCTTACAGCCCGGCGGTGAATCCTGGAGTGCATTACGTGCACGATTCGATCCGTGGCTGGTTGCCGAAGCCGAAAAAGAAGGTGTCGAATGCATCCCCGGAGCGACGGTGGa > 1:823180/1‑140 (MQ=255) cGCCCGATGGCGTAATGACGTTGTCCAGCTTACAGCCGGGCGGTGAATCCTGGAGTGCATTACGTGCACGATTCGATCCGTGGCTGGTTGCCGAAGCCGAAAAAGAAGGTGTCGAATGCATCCCCGGAGCGACGGTGGAt < 1:299076/140‑1 (MQ=255) gCCCGATGGCGTAACGACGTTTTCCAGCTTACAGCCCGGCGGTGAATCCTGGAGTGCATTACGTGCACGATTCGATCCGTGGCTGGTTGCCGAAGCCGAAAAAGAAGGTGTCGAATGCATCCCCGGAGCGACGGTGGATg < 1:707905/140‑1 (MQ=255) cccGATGGCGTAACGACGTTTTTCAGCTTCCAGCCCGTCGGTGAATCCTGGAGTGCATTACGTGCACGATTCGATCCGTGGCTGGTTGCCGAAGCCGAAAAAGAAGGTGTCGAATGCATCCCCGGAGCGACGGTGGATGc < 1:312082/140‑1 (MQ=255) cccGATGGCGTAACGACGTTTTCCAGCTTACCGCCCGGCGGTGAATCCCGGAGTGCATTACGTGCACGATTCGATCCGTGGCTGGTTGCCGAAGCCGAAAAAGAAGGTGTCGAATGCATCCCCGGAGCGACGGTGGATGc < 2:319499/140‑1 (MQ=255) ccGATGGCGTAACGACGTTTTCCAGCTTACAGCCCGGCGGTGAATCATGGAGTGCATTACGTGCACGATTCGATCCGTGGCTGGTTGCCGAAGCCGAAAAAGAAGGTGTCGAATGCATCCCCGGAGCGACGGTGGATGCa < 2:178976/140‑1 (MQ=255) gATGGCGTAACGACGTTTTCCAGCTTACAGCCCGGCGGTGAATCCTGGAGTGCATTACGTGCACGATTCGATCCGTGGCTGGTTGCCGAAGCCGAAAAAGGAGGCGTCAAATGCATCCCCGGAGCGACGGTGGATGCAcc > 2:354063/1‑139 (MQ=255) aTGGCGTAACGACGTTTTCCAGCTTACAGCCCGGCGGTGAATCCTGGAGTGCATTACGTGCACGATTCGATCCGTGGCTGGTTGCCGAAGCCGAAAAAGAAGGTGTCGAATGCATCCCCGGAGCGACgg < 2:860180/129‑1 (MQ=255) aTGGCGTAACGACGTTTTCCAGCTTACAGCCCGGCGGTGAATCCTGGAGTGCATTACGTGCACGATTCGATCCGTGGCTGGTTGCCGAAGCCGAAAAAGAAGGTGTCGAATGCATCCCCGGAGCGACgg > 1:860180/1‑129 (MQ=255) aTGGCGTAACGACGTTTTCCAGCTTACAGCCCGGCGGTGAATCCTGGAGTGCATTACGTGCACGATTCGATCCGTGGCTGGTTGCCGAAGCCGAAAAAGAAGGTGTCGAATGCATCCCCGGAGCGACGGTGGATGCa < 2:130896/137‑1 (MQ=255) aTGGCGTAACGACGTTTTCCAGCTTACAGCCCGGCGGTGAATCCTGGAGTGCATTACGTGCACGATTCGATCCGTGGCTGGTTGCCGAAGCCGAAAAAGAAGGTGTCGAATGCATCCCCGGAGCGACGGTGGATGCa > 1:130896/1‑137 (MQ=255) aaCGACGTTTTCCAGCTTACAGCCCGGCGGTGAATCCTGGAGTGCATTACGTGCACGATTCGATCCGTGGCTGGTTGCCGAAGCCGAAAAACAAGGTGTCGAATGCATACCCGGAGCAACGGAGGATGCACTGTATgaag > 2:245522/1‑140 (MQ=255) tttCCAGCTTACAGCCCGGCGGTGAATCCTGGAGTGCATTACGTGCACGATTCGATCCGTGGCTGGTTGCCGAAGCCGAAAAAGAAGGTGTCGAATGCATCCCCGGAGCGACGGTGGATGCACTGTATGAAGAAAACGGc < 2:823180/140‑1 (MQ=255) cTTACAGCCCGGCGGTGAATCCTGGAGTGCATTACGTGCACGATTCGATCCGTGGCTGGTTGCCGAAGCCGAAAAAGAAGGTGTCGAATGCATCCCCGGAGCGACGGTGGATGCACTGTATGAAGAAAACGGCAGAGTCt > 1:632071/1‑140 (MQ=255) aGCCCGGCGGTGAATCCTGGAGTGCATTACGTGCACGATTCGATCCGTGGCTGGTTGCCGAAGCCGAAAAAGAAGGTGTCGAATGCATCCCCGGAGCGACGGTGGATGCACTGTATGAAGAAAACGGCAGAGTCTGTGGc < 1:545641/140‑1 (MQ=255) cggcggTGAATCCTGGAGTGCATTACGTGCACGATTCGATCCGTGGCTGGTTGCCGAAGCCGAAAAAGAAGGTGTCAAATGCATCCCCGGAGCGACGGTGGATGCACTGTATGAAGAAAACGGCAGAGTCTGTGGCGTTa > 1:89482/1‑140 (MQ=255) gcggTGAATCCTGGAGTGCATTACGTGCACGATTCGATCCGTGGCTGGTTGCCGAAGCCGAAAAAGAAGGTGTCGAATGCATCCCCGGAGCGACGGTGGATGCACTGTATGAAGAAAACGGCAGAGTCTGTGGCGTTAtt > 2:15654/1‑140 (MQ=255) gAATCCTGGAGTGCATTACGTGCACGATTCGATCCGTGGCTGGTTGCCGAAGCCGAAAAAGAAGGTGTCGAATGCATCCCCGGAGCGACGGTGGATGCACTGTATGAAGAAAACGGCAGAGTCTGTGGCGTTATTTgtgg < 1:791527/140‑1 (MQ=255) tGGAGTGCATTACGTGCACGATTCGATCCGTGGCTGGTTGCCGAAGCCGAAAAAGAAGGTGTCGAATGCATCCCCGGAGCGACGGTGGATGCACTGTATGAAGAAAACGGCAGAGTCTGTGGCGTCATTTGTGGTGACGa > 2:677026/1‑140 (MQ=255) | CGCGGAACTCCTCCCGCAATTTCATCTGACCGCGCCTCTTGAACGACGCATCACTCACGAAAGCCTTTCCCTGTTAACGCCCGATGGCGTAACGACGTTTTCCAGCTTACAGCCCGGCGGTGAATCCTGGAGTGTATTACGTGCACGATTCGATCCGTGGCTGGTTGCCGAAGCCGAAAAAGAAGGTGTCGAATGCATCCCCGGAGCGACGGTGGATGCACTGTATGAAGAAAACGGCAGAGTCTGTGGCGTTATTTGTGGTGACGA > NC_000913/2892818‑2893084 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |