Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,909,569	C→T	G33D (GGC→GAC)	pyrG ←	CTP synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,909,569	0	C	T	100.0%	132.6 / NA	43	G33D (GGC→GAC)	pyrG	CTP synthetase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (26/17);  total (26/17)

CCCCAGGTCCAGGTCGGTTTCAGCGCCGTCTTCAGTAACGAACACTTCCCCGTGTTGGATTGGGCTCATAGTACCTGGATCGACGTTGATGTACGGATCCAGTTTCATGATGGTCACATTGAGGCCACGGGCTTCAAGAATGGCTGCGAGGGAGGCTGCGGCAATGCCTTTACCCAGAGAGGATACGACCCCGCCGGTCACAAAAATATAGTTCGTTGTCATGCTGAACCTGAGAAGTTAGGTTGAAAAGACGATGGAATA  >  NC_000913/2909445‑2909705                                                                                                                             |                                                                                                                                         ccccAGGTCCAGGTCGGTTTCAGCGCCGTCTTCAGTAACGAACACTTCCCCGTGTTGGATTGGGCTCATAGTACCTGGATCGACGTTGATGTACGGATCCAGTTTCATGATGGTCACATTGAGGTCACGGGCTTCAAGaa                                                                                                                           >  2:135557/1‑140 (MQ=255)     aggtccaggtcGGTTTCAGCGCCGTCTTCAGTAACGAACACTTCCCCGTGTTGGATTGGGCTCATAGTACCTGGATCGACGTTGATGTACGGATCCAGTTTCATGATGGTCACATTGAGGTCACGGGCTTCAAGAATGGc                                                                                                                       >  2:850537/1‑140 (MQ=255)       gtccaggtcGGTTTCAGCGCCGTCTTCAGTAACGAACACTTCCCCGTGTTGGATTGGGCTCATAGTACCTGGATCGACGTTGATGTACGGAGCCAGTTTCATGACGGTGACATTGAGTTCACCGGCTTCAAGAATGGCTg                                                                                                                     >  2:277978/1‑140 (MQ=255)            ggtcGGTTTCAGCGCCGTCTTCAGTAACGAACACTTCCCCGTGTTGGATTGGGCTCATAGTACCTGGATCGACGTTGATGTACGGATCCAGTTTCATGATGGTCACATTGAGGTCACGGGCTTCAAGAATGGCTGCgagg                                                                                                                >  1:510193/1‑140 (MQ=255)              tcGGTTTCAGCGCCGTCTTCAGTAACGAACACTTCCCCGTGTTGGATTGGGCTCATAGTACCTGGATCGACGTTGATGTACGGATCCAGTTTCATGATGGTCACATTGAGGTCACGGGCTTCAAGAATGGCTGCgaggga                                                                                                              >  1:624939/1‑140 (MQ=255)                  tttCAGCGCCGTCTTCAGTAACGAACACTTCCCCGTGTTGGATTGGGCTCATAGTACCTGGATCGACGTTGATGTACGGATCCAGTTTCATGATGGTCACATTGAGGTCACGGGCTTCAAGAATGGCTGCGAGGGAGGCt                                                                                                          <  2:510193/140‑1 (MQ=255)                       gcgcCGTCTTCAGTAACGAACACTTCCCCGTGTTGGATTGGGCTCATAGTACCTGGATCGACGTTGATGTACGGATCCAGTTTCATGATGGTCACATTGAGGTCACGGGCTTCAAGAATGGCTGCGAGGGAGGCTGCGGc                                                                                                     <  1:850537/140‑1 (MQ=255)                       gcgcCGTCTTCAGTAACAAACATTTCCCCGTGTTGTATTTGGCTCATAATACCTGGATCGACGTTGATGTACGGATCCAGTTTCATGATGGTCACATTGAGGTCACGGGCTTCAAGAATGGCTGCGAGGGAGGCTGTGGc                                                                                                     <  2:337323/140‑1 (MQ=255)                             tCTTCAGTAACGAACACTTCCCCGTGTTGGATTGGGCTCATAGTACCTGGATCGACGTTGATGTACGGATCCAGTTTCATGATGGTCACATTGAGGTCACGGGCTTCAAGAATGGCTACGAGGGAGGCTGCGGCAATGcc                                                                                               >  1:350672/1‑140 (MQ=255)                                    tAACGAACACTTCCCCGTGTTGGATTGGGCTCATAGTACCTGGATCGACGTTGATGTACGGATCCAGTTTCATGATGGTCACATTGAGGTCACGGGCTTCAAGAATGGCTGCGAGGGAGGCTGCGGCAATGCCTTTAccc                                                                                        >  1:567910/1‑140 (MQ=255)                                       cGAACACTTCCCCGTGTTGGATTGGGCTCATAGTACCTGGATCGACGTTGATGTACGGATCCAGTTTCATGATGGTCACATTGAGGTCACGGGCTTCAAGAATGGCTGCGAGGGAGGCTGCGGCAATGCCTTTACCCaga                                                                                     <  2:624939/140‑1 (MQ=255)                                            acTTCCCCGTGTTGGATTGGGCTCATAGTACCTGGATCGACGTTGATGTACGGATCCAGTTTCATGATGGTCACATTGAGGTCACGGGCTTCAAGAATGGCTGCGAGGGAGGCTGCGGCAATGCCTTTACCCAGAGAGGa                                                                                <  1:537639/140‑1 (MQ=255)                                                ccccGTGTTGGATTGGGCTCATAGTACCTGGATCGACGTTGATGTACGGATCCAGTTTCATGATGGTCACATTGAGGTCACGGGCTTCAAGAATGGCTGCGAGGGAGGCTGCGGCAATGCCTTTACCCAGAGAGGATACg                                                                            >  2:442644/1‑140 (MQ=255)                                                ccccGTGTTGGATTGGGCTCATAGTACCTGGATCGACGTTGATGTACGGATCCAGTTTCATGATGGTCACATTGAGGTCACGGGCTTCAAGAATGGCTGCGAGGGAGGCTGCGGCAATGCCTTTACCCAGAGAGGATACg                                                                            >  1:196047/1‑140 (MQ=255)                                                   cGTGTTGGATTGGGCTCATAGTACCTGGATCGACGTTGATGTACGGATCCAGTTTCATGATGGTCACATTGAGGTCACGGGCTTCAAGAATGGCTGCGAGGGAGGCTGCGGCAATGCCTTTACCCAGAGAGGATACGAcc                                                                         >  1:150434/1‑140 (MQ=255)                                                   cGTGTTGGATTGGGCTCATAGTACCTGGATCGACGTTGATGTACGGATCCAGTTTCATGATGGTCACATTGAGGTCACGGGCTTCAAGAATCGCTGCGAGGGAGGCGGCGGCAATGCCTTTAGACAGAGAGGATACGAcc                                                                         >  2:387892/1‑140 (MQ=255)                                                     tgtTGGATTGGGCTCATAGTACCTGGATCGACGTTGATGTACGGATCCAGTTTCATGATGGTCACATTGAGGTCACGGGCTTCAAGAATGGCTGCGAGGGAGGCTGCGGCAATGCCTTTACCCaga                                                                                     <  2:643621/126‑1 (MQ=255)                                                     tgtTGGATTGGGCTCATAGTACCTGGATCGACGTTGATGTACGGATCCAGTTTCATGATGGTCACATTGAGGTCACGGGCTTCAAGAATGGCTGCGAGGGAGGCTGCGGCAATGCCTTTACCCaga                                                                                     >  1:643621/1‑126 (MQ=255)                                                     tgtTGGATTGGGCTCATAGTACCTGGATCGACGTTGATGTACGGATCCAGTTTCATGATGGTCACATTGAGGTCACGGGCTTCAAGAATGGCTGCGAGGGAGGCTGCGGCAATGCCTTTACCCAGAGAGGATACGAcccc                                                                       >  1:149779/1‑140 (MQ=255)                                                             tGGGCTCATAGTACCTGGATCGACGTTGATGTGCGGATCCGGTTTCATGATGGTCACATTGAGGTCACGGGCTTCAAGAATGGCTGCGAGGGAGGCTGCGGCAATGCCTTTCCCCAGAGAGGATACGACCCCGCCGGTca                                                               <  2:693168/140‑1 (MQ=255)                                                                    aTAGTACCTGGATCGACGTTGATGTACGGATCCAGTTTCATGATGGTCACATTGAGGTCACGGGCTTCAAGAATGGCTGCGAGGGAGGTTGCGGCAATGCCTTTACCCAGAGAGGATACGACCCCGCCGGt                                                                 <  2:158682/131‑1 (MQ=255)                                                                    aTAGTACCTGGATCGACGTTGATGTACGGATCCAGTTTCATGATGGTCACATTGAGGTCACGGGCTTCAAGAATGGCTGCGAGGGAGGCTGCGGCAATGCCTTTACCCAGAGAGGATACGACCCCGCCGGt                                                                 >  1:158682/1‑131 (MQ=255)                                                                    aTAGTACCTGGATCGACGTTGATGTACGGATCCAGTTTCATGATGGTCACATTGAGGTCACGGGCTTCAAGAATGGCTGCGAGGGAGGCTGCGGCAATGCCTTTACCCAGAGAGGATACGACCCCGCCGGTCACAAAAat                                                        >  2:457448/1‑140 (MQ=255)                                                                    aTAGTACCTGGATCGACGTTGATGTACGGATCCAGTTTCATGATGGTCACATTGAGGTCACGGGCTTCAAGAATGGCTGCGAGGGAGGCTGCGGCAATGCCTTTACCCAGAGAGGATACGACCCCGCCGGTCACAAAAat                                                        >  2:18034/1‑140 (MQ=255)                                                                     tAGTACCTGGATCGACGTTGATGTACGGATCCAGTTTCATGATGGTCACATTGAGGTCACGGGCTTCAAGAATGGCTGCGAGGGAGGCTGCGGCAATGCCTTTACCCAGAGAGGATACGACCCCGCCGGTCACAAAAata                                                       <  1:442644/140‑1 (MQ=255)                                                                         aCCTGGATCGACGTTGATGTACGGATCCAGTTTCATGATGGTCACATTGAGGTCACGGGCTTCAAGAATGGCTGCGAGGGAGGCTGCGGCAATGCCTTTACCCAGAGAGGATACGACCCCGCCGGTCACAAAAATATAGt                                                   >  1:137594/1‑140 (MQ=255)                                                                          ccTGGATCGACGTTGATGTACGGATCCAGTTTCATGATGGTCACATTGAGGTCACGGGCTTCAAGAATGGCTGCGAGGGAGGCTGCGGCAATGCCTTTACCCAGAGAGGATACGACCCCGCCGGTCACAAAAATATAGtt                                                  <  1:457448/140‑1 (MQ=255)                                                                              gATCGACGTTGATGTACGGATCCAGTTTCATGATGGTCACATTGAGGTCACGGGCTTCAAGAATGGCTGCGAGGGAGGCTGCGGCAATGCCTTTACCCAGAGAGGATACGGCCCCGCCGGTCACAAAAATATAGTTCGtt                                              >  1:314698/1‑140 (MQ=255)                                                                              gATCGACGTTGATGTACGGATCCAGTTTCATGATGGTCACATTGAGGTCACGGGCTTCAAGAATGGCTGCGAGGGAGGCTGCGGCAATGCCTTTACCCAGAGAGGATACGACCCCGCCGGTCACAAAAATATAGTTCGtt                                              >  1:194980/1‑140 (MQ=255)                                                                                tCGACGTTGATGTACGGCTCCATTTTCATGATGGTCACAGTGAGGTCATGGGCTTCAAGAATGGCTGCGAGGGAGGCTGCGGCAATGCCTTTACCCAGAGAGGATACGACCCCGCCGGTCACAAAAATATAGTTCGTTGt                                            <  2:314698/140‑1 (MQ=255)                                                                                tCGACGTTGATGTACAGATCCACTTTCATAATGGTCAAATTGAGGTCACGGGCTTCAAGAATGGCTGCGAGGGAGGCTGCGGCAATGCCTTTACCCAGAGAGGATACGCCCCCGCCGGTCACAAAAATATAGTTCGTTGt                                            <  1:387892/140‑1 (MQ=255)                                                                                  gACGTTGATGTACGGATCCAGTTTCATGATGGTCACATTGAGGTCACGGGCTTCAAGAATGGCTGCGAGGGAGGCTGCGGCAATGCCTTTACCCAGAGAGCATAGGACGCCGCCGGTTACAAAAATATAGTTCGTTGTCa                                          >  2:310545/1‑140 (MQ=255)                                                                                    cGTTGATGTACGGATCCAGTTTCATGATGGTCACATTGAGGTCACGGGCTTCAAGAATGGCTGCGAGGGAGGCTGCGGCAATGCCTTTACCCAGAGAGGATACGACCCCGCCGGTCACAAAAATATAGTTCGTTGTCATg                                        >  2:598436/1‑140 (MQ=255)                                                                                    cGTTGATGTACGGATCCAGTTTCATGATGGTCACATTGAGGTCACGGGCTTCAAGAATGGCTGCGAGGGAGGCTGCGGCAATGCCTTTACCCAGAGAGGATACGACCCCGCCGGTCACAAAAATATAGTTCGTTGTCATg                                        <  2:137594/140‑1 (MQ=255)                                                                                      ttGATGTACGGATCCAGTTTCATGATGGTCACATTGAGGTCGCGGGCTTCAAGAATGGCTGCGAGGGAGGCTGCGGCAATGCCTTTACCCAGAGAGGATACGACCCCGCCGGTCACAAAAATATAGTTCGTTGTCATGCt                                      <  1:588862/140‑1 (MQ=255)                                                                                       tGATGTACGGATCCAGTTTCATGATGGTCACATTGAGGTCACGGGCTTCAAGAATGGCTGCGAGGGAGGCTGCGGCAATGCCTTTACCCAGAGAGGATACGCCCCCGCCGGTCACAAAAATATAGTTCGTTGTCATGCTg                                     <  2:150434/140‑1 (MQ=255)                                                                                       tGATGTACGGATCCAGTTTCATGATGGTCACATTGAGGTCACGGGCTTCAAGAATGGCTGCGAGGGAGGCTGCGGCAATGCCTTTACCCAGAGAGGATACGACCCCGCCGGTCACAAAAATATAGTTCGTTGTCATGCTg                                     <  2:149779/140‑1 (MQ=255)                                                                                        gATGTACGGATCCAGTTTCATGATGGTCACATTGAGGTCACGGGCTTCAAGAATGGCTGCGAGGGAGGCTGCGGCAATGCCTTTACCCAGAGAGGATACGACCCCGCCGGTCACAAAAATATAGTTCGTTGTCATGCTGa                                    >  2:44431/1‑140 (MQ=255)                                                                                             aCGGATCCAGTTTCATGATGGTCACATTGAGGTCACGGGCTTCAAGAATGGCTGCGAGGGAGGCTGCGGCAATGCCTTTACCCAGAGAGGATACGACCCCGCCGGTCACAAAAATATAGTTCGTTGTCATGCTGAACCTg                               <  1:740639/140‑1 (MQ=255)                                                                                                   ccAGTTTCATGATGGTCACATTGAGGTCACGGGCTTCAAGAATGGCTGCGAGGGAGGCTGCGGCAATGCCTTTACCCAGAGAGGATACGACCCCGCCGGTCACAAAAATATAGTTCGTTGTCATGCTGAACCTGAGAAGt                         >  2:555557/1‑140 (MQ=255)                                                                                                                 gTCACATTGAGGTCACGGGCTTCAAGAATGGCTGCGAGGGAGGCTGCGGCAATGCCTTTACCCAGAGAGGATACGACCCCGCCGGTCACAAAAATATAGTTCATTGTCATACTGAACATGAGAACTTAGGTTGAAAAGAc           >  1:286516/1‑140 (MQ=255)                                                                                                                     caTTGAGGTCACGGGCTTCAAGAATGGCTGCGAGGGAGGCTGCGGCAATGCCTTTACCCAGAGAGGATACGACCCCGCCGGTCACAAAAATATAGTTCGTTGTCATGCTGAACCTGAGAAGTTAGGTTGAAAAGACGATg       >  2:596131/1‑140 (MQ=255)                                                                                                                          aGGTCACGGGCTTCAAGAATGGCTGCGAGGGAGGCTGCGGCAATGCCTTTACCCAGAGAGGATACGACCCCGCCGGTCACAAAAATATAGTTCGTTGTCATGCTGAACCTGAGAAGTTAGGTTGAAAAGACGATGGAATa  <  1:135557/140‑1 (MQ=255)                                                                                                                          aGGTCACGGGCTTCAAGAATGGCTGCGAGGGAGGCTGCGGCAATGCCTTTACCCAGAGAGGATACGACCCCGCCGGTCACAAAAATATAGTTCGTTGTCATGCTGAACCTGAGAAGTTAGGGTGAAAAGACGATGGAATa  >  1:858621/1‑140 (MQ=255)                                                                                                                             |                                                                                                                                         CCCCAGGTCCAGGTCGGTTTCAGCGCCGTCTTCAGTAACGAACACTTCCCCGTGTTGGATTGGGCTCATAGTACCTGGATCGACGTTGATGTACGGATCCAGTTTCATGATGGTCACATTGAGGCCACGGGCTTCAAGAATGGCTGCGAGGGAGGCTGCGGCAATGCCTTTACCCAGAGAGGATACGACCCCGCCGGTCACAAAAATATAGTTCGTTGTCATGCTGAACCTGAGAAGTTAGGTTGAAAAGACGATGGAATA  >  NC_000913/2909445‑2909705

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑