| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,910,165 | T→C | D174G (GAC→GGC) | mazG ← | nucleoside triphosphate pyrophosphohydrolase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,910,165 | 0 | T | C | 95.5% | 66.7 / ‑4.5 | 22 | D174G (GAC→GGC) | mazG | nucleoside triphosphate pyrophosphohydrolase |
| Reads supporting (aligned to +/- strand): ref base T (0/1); new base C (12/9); total (12/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.55e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01 | |||||||||||
CTTTTTGCAATGCGATTTCTGCTTTCGTCCCTAAATGGCGAGCCAGATTAACCGTGGCAAACAGCAGGTCCCCCATTTCCTCCTCCAGTTTAGCCTGGTCGACAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAGACTTTATCGACTACCGGACCAAGCGTCGTCCAATCGAAGCCAACGTTGGCGCAACGTTTCTGGATTTTTTGCGCACGCATTAAAGCCGGTAAACTACGAGGAATATCGTCCAGCGCCGAAT > NC_000913/2910031‑2910300 | cTTTTTGCAATGCGATTTCTGCTTTCGTCCCTAAATGGCGAGCCAGATTAACCGTGGCAAACAGCAGGTCCCCCATTTCCTCCTCCAGTTTAGCCTGGTCGACAACAGCCTGCCGCGCTTCGTACATCCCCTCGCCGAtc < 1:588040/140‑1 (MQ=255)cTTTTTGCAATGCGATTTCTGCTTTCGTCCCTAAATGGCGAGCCAGATTAACCGTGGCAAACAGCAGGTCCCCCATTTCCTCCTCCAGTTTAGCCTGGTCGACAACAGCCTGCCGCGCTTCGTACATCACCTCGCCGAtc > 1:615675/1‑140 (MQ=255) tGCAATGCGATTTCTGCTTTCGTCCCTAAATGGCGAGCCAGATTAACCGTGGCAAACAGCAGGTCCCCCATTTCCTCCTCCAGTTTAGCCTGGTCGACAACAGCCTGCCGCGCTTCGTACATCACCTCGCCGATCTCTTc < 2:484107/140‑1 (MQ=255) aaTGCGATTTCTGCTTTCGTCCCTAAATGGCGAGCCAGATTAACCGTGGCAAACAGCAGGTCCCCCATTTCCTCCTCCAGTTTAGCCTGGTCGACAACAGCCTGCCGCGCTTCGTACATCACCTCGCCGATCTCTTCGTa < 1:631658/140‑1 (MQ=255) tttCTGCTTTCGTCCCTAAATGGCGAGCCAGTTTAACCGGGGCAAACAGCAGGTCCCCCATTTCCTCCTCCAGTTTAGCCTGGTCGACAACAGCCTGCCGCGCTTCGTACATCACCTCGCCGATCTCTTCGTAGACTTTa < 2:294436/140‑1 (MQ=255) tttCTGCTTTCGTCCCTAAATGGCGAGCCAGATTAACCGTGGCAACCAGCAGGTCCCCCATTTCCTCCTCCAGTTTAGCCTGGTCGACAACAGCCTGCCGCGCTTCGTACATCACCTCGCCGATCTCTTCGTAGACTTTa < 2:834110/140‑1 (MQ=255) gAGCCAGATTAACCGTGGCAAACAGCAGGTCCCCCATTTCCTCCTCCAGTTTAGCCTGGTCGACAACAGCCTGCCGCGCTTCGTACATCACCTCGCCGATCTCTTCGTAGACTTTATCGACTACCGGACCAAGCgtcgtc < 2:86074/140‑1 (MQ=255) ccAGATTAACCGTGGCAAACAGCAGGTCCCCCATTTCCTCCTCCAGTTTAGCCTGGTCGACAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAGACTTTATCGACTACCGGACCAAGCGTCGTCCaa < 2:192386/140‑1 (MQ=255) aCCGTGGCAAACAGCAGGTCCCCCATTTCCTCCTCCAGTTTAGCCTGGTCGACAACAGCCTGCCGCGCTTCGTACATCACCTCGCCGATCTATTCGTAGAATTTATCGGCTACCGGACCAAGCGTCGTCCAATCGAAGcc > 2:345943/1‑140 (MQ=255) gCAAACAGCAGGTCCCCCATTTCCTCCTCCAGTTTAGCCTGGTCGACAACAGCCTGCCGCGCTTCGTACATCACCTCGCCGATCTCTTCGTAGACTTTATCGACTACCGGACCAAGCGTCGTCCAATCGAAGCCAACGtt > 2:480826/1‑140 (MQ=255) ccATTTCCTCCTCCAGTTTAGCCTGGTCGACAACAGCCTGCCGCGCTTCGTACATCACCTCGCCGATCTCTTCGTAGACTTTATCGACTACCGGACCAAGCGTCGTCCAATCGAAGCTAACGTTAGCGCAACGTTTCTgg > 1:416046/1‑140 (MQ=255) cctccAGTTTAGCCTGGTCGACAACAGCCTGCCGCGCTTCGTACATCACCTCGCCGATCTCTTCGTAGACTTTATCGACTACCGGACCAAGCGTCGTCCAATCGAAGCCAACGTTGGCGCAACGTTTCTGGATTTTTTgc > 2:593794/1‑140 (MQ=255) cctccAGTTTAGCCTGGTCGACAACAGCCTGCCGCGCTTCGTACATCACCTCGCCGATCTATTCGTAGACTTTATCGACTACCGGACCAAGAGTCGTCCAGTCGAAGCCAACGTTGGAGCAATGTTTCTGGATTTTTTgc > 2:321633/1‑140 (MQ=255) cgcgTTTACCGTGGTCGCCACTAGTCTGCCGCTTTGCGAACATCACCTCGCCGCTCTCTACGTAGACTTTATCTGTTTGCGGACCAATCTCCGTCCAATCGAAGCCAACGTTGGCGCAACGTTTCTTGAATTTTTGcgca < 2:535173/137‑1 (MQ=255) ccAGTTTAGCCTGGTGGACAACCGCCTGCCGCGCTTCGTACATCACCTCGCCGATCTCTTCGTAGACTTTATCGTCTACCGGACCAAGCGTCGTCCAATCGAAGCCAACGTTGGCGCAACGTTTCTGGATTTTTTGcgca < 1:345943/140‑1 (MQ=255) ggTCGACAACAGCCTGCCGCGCTTCGTACATCACCTCGCCGATCTCTTCGTAGACTTTATCGACTACCGGACCAAGCGTCGTCCAATCGAAGCCAACGTTGGCGCAACGTTTCTGGATTTTTTGCGCACGCATTAAAGcc < 2:615675/140‑1 (MQ=255) gTCGACAACAGCCTGCCGCGCTTCGTACATCACCTCGCCGATCTCTTCGTAGACTTTATCGACTACCGGACCAAGCGTCGTCCAATCGAAGCCAACGTTGGCGCAACGTTTCTGGATTTTTTGCGCACGCATTAAAGCCg > 1:698690/1‑140 (MQ=255) acaGCCTGCCGCGCTTCGTACATCACCTCGCCGATCTCTTCGTAGACTTTATCGACTACCGGACCAAGCGTCGTCCAATCGAAGCCAACGTTGGCGCAACGTTTCTGGATTTTTTGCGCACGCATTAAAGCCGGTAAACt < 2:708175/140‑1 (MQ=255) ccTGCCGCGCTTCGTACATCACCTCGCCGATCTCTTCGTAGACTTTATCGACTACCGGACCAAGCGTCGTCCAATCGAAGCCAACGTTGGCGCAACGTTTCTGGATTTTTTGCGCACGCATTAAAGCCGGTAAACTACGa > 1:447490/1‑140 (MQ=255) ccTGCCGCGCTTCGTACATCACCTCGCCGATCTCTTCGTAGACTTTATCGACTACCGGACCAAGCGTCGTCCAATCGAAGCCAACGTTGGCGCAACGTTTCTGGATTTTTTGCGCACGCATTAAAGCCGGTAAACTACGa > 1:442064/1‑140 (MQ=255) ttCGTACATCACCTCGCCGATCTCTTCGTAGACTTTATCGACTACCGGACCAAGCGTCGTCCAATCGAAGCCAACGTTGGCGCAACGTTTCTGGATTTTTTGCGCACGCATTAAAGCCGGTAAACTACGAGGAATATCGt > 1:160164/1‑140 (MQ=255) aCCTCGCCGATCTCTTCGTAGACTTTATCGACTACCGGACCAAGCGTCGTCCAATCGAAGCCAACGTTGGCGCAACGTTTCTGGATTTTTTGCGCACGCATTAAAGCCGGTAAACTACGAGGAATATCGTCCAGCGCCGa > 1:392108/1‑140 (MQ=255) cTCGCCGATCTCTTCGTAGACTTTATCGACTACCGGACCAAGCGTCGTCCAATCGAAGCCAACGTTGGCGCAACGTTTCTGGATTTTTTGCGCACGCATTAAAGCCGGTAAACTACGAGGAATATCGTCCAGCGCCGAAt > 2:715467/1‑140 (MQ=255) | CTTTTTGCAATGCGATTTCTGCTTTCGTCCCTAAATGGCGAGCCAGATTAACCGTGGCAAACAGCAGGTCCCCCATTTCCTCCTCCAGTTTAGCCTGGTCGACAACAGCCTGCCGCGCTTCGTACATCACCTCGTCGATCTCTTCGTAGACTTTATCGACTACCGGACCAAGCGTCGTCCAATCGAAGCCAACGTTGGCGCAACGTTTCTGGATTTTTTGCGCACGCATTAAAGCCGGTAAACTACGAGGAATATCGTCCAGCGCCGAAT > NC_000913/2910031‑2910300 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |