Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,920,251	T→C	N166D (AAT→GAT)	gudX ←	glucarate dehydratase‑related protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,920,251	0	T	C	100.0%	99.0 / NA	30	N166D (AAT→GAT)	gudX	glucarate dehydratase‑related protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (14/16);  total (14/16)

AGGTAACACGCCGCCCTTAAGTTTGAAATCTTTAAAGCCGTAGCGATCCTGTGAGGCTTCCGCCAGACGCACAACGGCTTCGCTGTTCATTGCTTTCTGATGCCGCAACTGATACCACTCATGGTTGCCCGGCGTATTTTCCACATAAGGAAGATCGGTTTTGGTCCGATCACCGATATAAAACAGATAACCGAGGACGGTAATAGCCTCGCGTTGCTTGCCTGGCCCTAACAGTTCGCAGACCGGAACATTCAGCGCCTTACCTAGCAGGT  >  NC_000913/2920114‑2920385                                                                                                                                          |                                                                                                                                       aGGTAACACGCCGCCCTTAAGTTTGAAATCTTTAAAGCCGTAGCGATCCTGTGAGGCTTCCGCCAGACGCACAACGGCTTCGCTGTTCATTGCTTTCTGATGCCGCAACTGATACCACTCATGGTTGCCCGGCGTAtctt                                                                                                                                      <  2:99082/140‑1 (MQ=255)    tAACACGCCGCCCTTAAGTTTGAAATCTTTAAAGCCGTAGCGATCCTGTGAGGCTTCCGCCAGACGCACAACGGCTTCGCTGTTCATTGCTTTCTGATGCCGCAACTGATACCACTCATGGTTGCCCGGCGTATCTTCca                                                                                                                                   <  2:461560/140‑1 (MQ=255)        acGCCGCCCTTAAGTTTGAAATCTTTAAAGCCGTAGCGATCCTGTGAGGCTTCCGCCAGACGCACAACGGCTTCGCTGTTCATTGCTTTCTGATGCCGCAACTGATACCACTCATGGTTGCCCGGCGTATCTTCCACATa                                                                                                                               <  1:824105/140‑1 (MQ=255)           ccgccCTTAAGTTTGAAATCTTTAAAGCCGTAGCGATCCTGTGAGGCTTCCGCCAGACGCACAACGGCTTCGCTGTTCATTGCTTTCTGATGCCGCAACTGATACCACTCATGGTTGCCCGGCGTATCTTCCACATAAgg                                                                                                                            <  1:609289/140‑1 (MQ=255)             gccCTTAAGTTTGAAATCTTTAAAGCCGTAGCGATCCTGTGAGGCTTCCGCCAGACGCACAACGGCTTCGCTGTTCATTGCTTTCTGATGCCGCAACTGATACCACTCATGGTTGCCCGGCGTATCTTCCACATAAGGaa                                                                                                                          >  1:198817/1‑140 (MQ=255)                             tCTTTAAAGCCGTAGCGCTCCTCTGAGGCTCTCGCAAGACGCACAACGGCTTCGCTGTTCATTGCTTTCTGATGCCGCAACTGATACCACTCATGGTTGCCCGGCGTATCTTCCACATAATGAAGATCGGTTTTGGTCCg                                                                                                          <  2:415689/140‑1 (MQ=255)                              cTTTAAAGCCGTAGCGATCCTGTGAGGCTTCCGCCAGACGCACAACGGCTTCGCTGTTCATTGCTTTCTGATGCCGCAACTGATACCACTCATGGTTGCCCGGCGTATCTTCCACATAAGGAAGATCGGTTTTGGTCCGa                                                                                                         <  1:810350/140‑1 (MQ=255)                                  aaaGCCGCACCTACCCTGTGAGGCTTCCGCCAGACGCACAACGATTTCGCTGTTCATTGCTTTCTGATGCCGCAACTGATACCACTCATGGTTGCCCGGCGTATCTTCCACATAAGGAAGATCGGTTTTGGTCCGATCAc                                                                                                     <  1:540062/140‑1 (MQ=255)                                    aGCCGTAGCGATCCTGTGAGGCTTCCGCCAGACGCACAACGGCTTCGCTGTTCATTGCTTTCTGATGCCGCAACTGATACCACTCATGGTTGCCCGGCGTATCTTCCACATAAGGAAGATCGGTTTTGGTCCGATCAc                                                                                                     >  2:597264/1‑138 (MQ=255)                                    aGCCGTAGCGATCCTGTGAGGCTTCCGCCAGACGCACAACGGCTTCGCTGTTCATTGCTTTCTGATGCCGCAACTGATACCACTCATGGTTGCCCGGCGTATCTTCCACATAAGGAAGATCGGTTTTGGTCCGATCAc                                                                                                     <  1:597264/138‑1 (MQ=255)                                      ccGTAGCGATCCTGTGAGGCTTCCGCCAGACGCACAACGGCTTCGCTGTTCATTGCTTTCTGATGCCGCAACTGATACCACTCATGGTTGCCCGGCGTATCTTCCACATAAGGAAGATCGGTTTTGGTCCGATCACCGat                                                                                                 >  2:105584/1‑140 (MQ=255)                                                    tgAGGCTTCCGCCAGACGCACAACGGCTTCGCTGTTCATTGCTTTCTGATGCCGCAACTGATACCACTCATGGTTGCCCGGCGTATCTTCCACATAAGGAGGATAGGTATTGGTCCGATCACCGATATAAAACAGATAAc                                                                                   >  2:264064/1‑140 (MQ=255)                                                    tgAGGCTTCCGCCAGACGCACAACGGCTTCGCTGTTCATTGCTTTCTGATGCCGCAACTGATACCACTCATGGTTGCCCGGCGTATCTTCCACATAAGGAAGATCGGTTTTGGTCCGATCACCGATATAAAACAGATAAc                                                                                   >  1:118322/1‑140 (MQ=255)                                                           tCCGCCAGACGCACAACGGCTTCGCTGTTCATTGCTTTCTGATGCCGCAACTGATACCACTCATGGTTGCCCGGCGTATCTTCCACATAAGGAAGATCGGTTTTGGTCCGATCACCGATATAAAACAGATAACCGAGGAc                                                                            >  1:619515/1‑140 (MQ=255)                                                                        caACGGCTTCGCTGTTCATTGCTTTCTGATGCCGCAACTGATACCACTCATGGTTGCCCGGCGTATCTTCCACATAAGGAAGATCGGTTTTGGTCCGATCACCGATATAAAACAGATAACCGAGGACGGTAATAGCCTcg                                                               >  2:684543/1‑140 (MQ=255)                                                                                   cTGTTCATTGCTTTCTGATGCCGCAACTGATACCACTCATGGTTGCCCGGCGTATCTTCCACATAAGGAAGATCGGTTTTGGTCCGATCACCGATATAAAACAGATAACCGAGGACGGTAATAGCCTCGCGTTGCTTGcc                                                    >  2:817519/1‑140 (MQ=255)                                                                                    tGTTCATTGCTTTCTTATGCCGCAATTGACACCACTCTTGGTTGCCCGGCGTATCTTCCACATAAGGAAGATCGGTTTTGGTCCGATCACCGATATAAAACAGATAACCGAGGACGGTAATAGCCTCGCGTTGCTTGCCt                                                   <  1:269075/140‑1 (MQ=255)                                                                                    tGTTCATTGCTTTCTGATGCCGCAACTGATTCCACTCATGGTTGCCCGGCGTATCTTCCACATAAGGAAGATCGGTTTTGGTCCGATCACCGATATAAAACAGATAACCGAGGACGGTAATAGCCTCGCGTTGCTTGCCt                                                   <  2:118322/140‑1 (MQ=255)                                                                                           tGCTTTCTGATGCCGCATCTGATACCACTCAGGGTAGCCCGGCGTATCCTCCACATAAGGAAGATCGGTTTTGGTCCGATCACCGATATAAAACAGATAACCGAGGACGGTAATAGCCTCGCGTTGCTTGCCTGGCCCTa                                            <  2:403818/140‑1 (MQ=255)                                                                                                tCTGATGCCGCAACTGATACCACTCATGGTTGCCCGGCGTATCTTCCACATAAGGAAGATCGGTTTTGGTCCGATCACCGATATAAAACAGATAACCGAGGACGGTAATAGCCTAGCATTGCTTACCTGGCCCTAACAGt                                       >  1:403818/1‑140 (MQ=255)                                                                                                   gATGCCGCAACTGATACCACTCATGGTTGCCCGGCGTATCTTCCACATAAGGAAGATCGGTTTTGGTCCGATCACCGATATAAAACAGATAACCGAGGACGGTAATAGCCTCGCGTTGCTTGCCTGGCCCTAACAGTTCg                                    <  2:619515/140‑1 (MQ=255)                                                                                                       ccGCAACTGATACCACTCATGGCTGCCCGTCGTATCTCCCACATAAGGAAGATCGGTTTTGGTCCGATCACCGATATAAAACAGATAACCGAGGACGGTAATAGCCTCGCGTTGCTTGCCTGGCCCTAACAGTTCGCAGa                                <  1:264064/140‑1 (MQ=255)                                                                                                                 tACCACTCATGGTTGCGCGGCGTATCTTCCACATAAGGAAGATCGGTTTTGGTCCGATCACCGATATAAAACAGATAACCGAGGACGGTAATAGCCTCGCGTTGCTTGCCTGGCCCTAACAGTTCGCAGACCGGAACAtt                      <  1:794314/140‑1 (MQ=255)                                                                                                                  aCCACTCATAGTTGCCCGGCGTATCTTCCACATAAGGAAGATCGGTTTTGGTCCGATCACCGATATAAAACAGATAACCGAGGACGGTAATAGCCTCGCGTTGCTTGCCTGGCCCTAACAGTTCGCAGACCGGAACATTc                     >  2:204816/1‑140 (MQ=255)                                                                                                                   ccACTCATGGTTGCCCGGCGTATCTTCCACATAAGGAAGATCGGTTTTGGTCCGATCACCGATATAAAACAGATAACCGAGGACGGTAATAGCCTCGCGTTGCTTGCCTGGCCCTAACAGTTCGCAGACCGGAACATTCa                    <  1:105584/140‑1 (MQ=255)                                                                                                                           ggTTGCCCGGCGTATCTTCCACATAAGGAAGATCGGTTTTGGTCCGATCACCGATATAAAACAGATAACCGAGGACGGTAATAGCCTCGCGTTGCTTGCCTGGCCCTAACAGTTCGCAGACCGGAACATTCAGCGcc               >  1:821390/1‑137 (MQ=255)                                                                                                                           ggTTGCCCGGCGTATCTTCCACATAAGGAAGATCGGTTTTGGTCCGATCACCGATATAAAACAGATAACCGAGGACGGTAATAGCCTCGCGTTGCTTGCCTGGCCCTAACAGTTCGCAGACCGGAACATTCAGCGcc               <  2:821390/137‑1 (MQ=255)                                                                                                                                cccGGCGTATCTTCCACATAAGGAAGATCGGTTTTGGTCCGATCACCGATATAAAACAGATAACCGAGGACGGTAATAGCCTCGCGTTGCTTGCCTGGCCCTAACAGTTGGCAGACCGGAACATTCAGCGCCTTACCTAg       >  1:106126/1‑140 (MQ=255)                                                                                                                                 ccGGCGTATCTTCCACATAAGGAAGATCGGTTTTGGTCCGATCACCGATATATAACAGATAACCGAGGACGGTAATAGCCTCGCGTTGCTTGCCTGGCCCTAACAGTTCGCAGACCGGAACATTCAGCGCCTTACCTAGc      >  2:48152/1‑140 (MQ=255)                                                                                                                                 ccGGCGTATCTTCCACATAAGGAAGATCGGTTTTGGTCCGATCACCGATATAAAACAGATAACCGAGGACGGTAATAGCCTCGCGTTGCTTGCCTGGCCCTAACAGTTCGCAGACCGGAACATTCAGCGCCTTACCTAGc      >  2:7117/1‑140 (MQ=255)                                                                                                                                     cGTATCTTCCACATAAGGAAGATCGGTTTTGGTCCGATCACCGATATAAAACAGATAACCGAGGACGGTAATAGCCTCGCGTTGCTTGCCTGGCCCTAACAGTTCGCAGACCGGAACATTCAGCGCCTTACCTAGCAGGt  <  2:198817/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                       AGGTAACACGCCGCCCTTAAGTTTGAAATCTTTAAAGCCGTAGCGATCCTGTGAGGCTTCCGCCAGACGCACAACGGCTTCGCTGTTCATTGCTTTCTGATGCCGCAACTGATACCACTCATGGTTGCCCGGCGTATTTTCCACATAAGGAAGATCGGTTTTGGTCCGATCACCGATATAAAACAGATAACCGAGGACGGTAATAGCCTCGCGTTGCTTGCCTGGCCCTAACAGTTCGCAGACCGGAACATTCAGCGCCTTACCTAGCAGGT  >  NC_000913/2920114‑2920385

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑