Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	7,390	C→T	L190L (CTG→CTA)	yaaJ ←	putative transporter YaaJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	7,390	0	C	T	100.0%	137.8 / NA	41	L190L (CTG→CTA)	yaaJ	putative transporter YaaJ
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (25/16);  total (25/16)

ACCAAATGACGTGGGGAAGTTGCCCGATATTCATTACGCAAATTACCAGGCTGGTCAGTACCCAGATTATCGCCATCAACGGGACAAAGCCCTGCATGAGCCGGGCGACGCCATGAAGACCGCGAGTGATTGCCAGCAGAGTAAAGACAGCGAGAATAATGCCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCGA  >  NC_000913/7254‑7523                                                                                                                                         |                                                                                                                                      aCCAAATGACGTGGGGAAGTTGCCCGATATTCATTACGCAAATTACCAGGCTGGTCAGTACCCAGATTATCGCCATCAACGGGACAAAGCCCTGCATGAGCCAGGCGACGGCATGAAAACCGCGAGTGATTGCCAGTaga                                                                                                                                    >  1:380165/1‑140 (MQ=255)      aTGACGTGGGGAAGTTGCCCGATATTCATTACGCAAATTACCAGGCTGGTCAGTACCCAGATTATCGCCATCAACGGGACAAAGCCCTGCATGAGCCGGGCGACGCCATGAAGACCGCGAGTGATTGCCAGTAGAGTaaa                                                                                                                               >  1:558653/1‑140 (MQ=255)      aTGACGTGGGGAAGTTGCCCGATATTCATTACGCAAATTACCAGGCTGGTCAGTACCCAGATTATCGCCATCAACGGGACAAAGCCCTGCATGAGCCGGGCGACGCCATGAAGACCGCGAGTGATTGCCAGTAGAGTaaa                                                                                                                               >  2:1060/1‑140 (MQ=255)                    ttGCCTGATATTCATTGAGCAATATACCAGGCCTGTCAGTACCCCGATAATCGCCATCAACGGGACAAAGCCCTGCATGAGCCGGGCGACGCCATGAAGACCGCGAGTGATTGCGAGTAGAGTAAAGACAGCTAGaataa                                                                                                                 <  2:380165/140‑1 (MQ=255)                    ttGCCCGATATTCATTACGCAAATTACCAGGCTGGTCAGTACCCAGATTATCGCCATCAACGGGACAAAGCCCTGCATGAGCCGGGCGACGCCATGAAGACCGCGAGTGATTGCCAGTAGAGTAAAGACAGCGAGaataa                                                                                                                 <  1:1060/140‑1 (MQ=255)                    ttGCCCGATATTCATTACGCAAATTACCAGGCTGGTCAGTACCCAGATTATCGCCATCAACGGGACAAAGCCCTGCATGAGCCGGGCGACGCCATGAAGACCGCGAGTGATTGCCAGTAGAGTAAAGACAGCGAGaataa                                                                                                                 <  2:558653/140‑1 (MQ=255)                        ccGATATTCATTACGCAAATTACCAGGCTGGTCAGTACCCAGATTATCGCCATCAACGGGACAAAGCCCTGCATGAGCCGGGCGACGCCATGAAGACCGCGAGTGATTGCCAGTAGAGTAAAGACAGCGAGAATAATGcc                                                                                                             >  2:667023/1‑140 (MQ=255)                         agATATTCATTACGCAAATTACCAGGCTGGTCAGTACCCAGATTATCGCCATCAACGGGACAAAGCCCTGCATGAGCCGGGCGACGCCATGAAGACCGCGAGTGATTGCCAGTAGAGTAAAGACAGCGAGaataa                                                                                                                 <  2:837261/134‑1 (MQ=255)                         agATATTCATTACGCAAATTACCAGGCTGGTCAGTACCCAGATTATCGCCATCAACGGGACAAAGCCCTGCATGAGCCGGGCGACGCCATGAAGACCGCGAGTGATTGCCAGTAGAGTAAAGACAGCGAGaataa                                                                                                                 >  1:837261/2‑135 (MQ=255)                           atatTCATTACGCAAATTACCAGGCTGGTCAGTACCCAGATTATCGCCATCAACGGGACAAAGCCCTGCATGAGCCGGGCGACGCCATGAAGACCGCGAGTGATTGCCAGTAGAGTAAAGACAGCGAGAATAATGCCTGt                                                                                                          >  2:791939/1‑140 (MQ=255)                              ttCATTACGCAAATTACGAGGCTGGTCAGTACCCATATTTTCGCCATCTACGGGACAAAGCCCTGCATGAGCCGGGCGACGCCATGAAGACCGCGAGTGATTGCCAGTAGAGTAAAGACAGCGAGAATAATGCCTGTCAc                                                                                                       <  2:320021/140‑1 (MQ=255)                              ttCATTACGCAAATTACCAGGCTGGTCAGTACCCAGATTATCGCCATCAACGGGACAAAGCCCTGCATGAGCCGGGCGACGCCATGAAGACCGCGAGTGATTGCCAGTAGAGTAAAGACAGCGAGAATAATGCCTGTCAc                                                                                                       <  1:651245/140‑1 (MQ=255)                                          aTTACCAGGCTGGTCAGTACCCAGATTATCGCCATCAACGGGACAAAGCCCTGCATGAGCCGGGCGACGCCATGAAGACCGCGAGTGATTGCCAGTAGAGTAAAGACAGCGAGAGTACTGCCTGACACCAGCGGGGGaaa                                                                                           >  1:400243/1‑140 (MQ=255)                                               cAGGCTGGTCAGTACCCAGATTTTCGCCATCGACGCGACAAAGCCCTGCATGAGCCGGGCGACGCCATGAAGACCGCGAGTGATTGCCAGTAGAGTAAAGACAGCGAGAATAATGCCTGTCACCAGCGGGGGAAAATCaa                                                                                      <  2:400787/140‑1 (MQ=255)                                                  gCTGGTCAGTACCCAGATTATCGCCATCAACGGGACAAAGCCCTGCATGAGCCGGGCGACGCCATGAAGACCGCGAGTGATTGCCAGTAGAGTAAAGACAGCGAGAATAATGCCTGTCACCAGCGGGGGAAAATCAAAAg                                                                                   >  1:19270/1‑140 (MQ=255)                                                  gCTGGTCAGTACCCAGATTATCGCCATCAACGGGACAAAGCCCTGCATGAGCCGGGCGACGCCATGAAGACCGCGAGTGATTGCCAGTAGAGTAAAGACAGCGAGAATAATGCCTGTCACCAGCGGGGGAAAATCAAAAg                                                                                   >  1:485050/1‑140 (MQ=255)                                                   cTGGTCAGTACCCAGATTATCGCCATCAACGGGACAAAGCCCTGCATGAGCCGGGCGACGCCATGAAGACCGCGAGTGATTGCCAGTAGAGTAAAGACAGCGAGAATAATGCCTGTCACCAGCGGGGGAAAATCAAAAGa                                                                                  >  2:568618/1‑140 (MQ=255)                                                      gTCAGTACCCAGATTATCGCCATCAACGGGACAAAGCCCTGCATGAGCCGGGCGACGCCATGAAGACCGCGAGTGATTGCCAGTAGAGTAAAGACAGCGAGACTAATGCCTGTCTCCTGCGGGGCAAAATCAAAAGaaaa                                                                               >  1:400787/1‑140 (MQ=255)                                                      gTCAGTACCCAGATTATCGCCATCAACGGGACAAAGCCCTGCATGAGCCGGGCGACGCCATGAAGACCGCGAGTGATTGCCAGTAGAGTAAAGACAGCGAGAATAATGCCTGTCTCCAGCGGGGAAAAATCAAAAGaaaa                                                                               >  1:341924/1‑140 (MQ=255)                                                      gTCAGTACCCAGATTATCGCCATCAACGGGACAAAGCCCTGCATGAGCCGGGCGACGCCATGAAGACCGCGAGTGATTGCCAGTAGAGTAAAGACAGCGAGAATAATGCCTGTCACCAGCGGGGGAAAATCAAAAGaaaa                                                                               >  1:659274/1‑140 (MQ=255)                                                      gTCAGTACCCAGATTATCGCCATCAACGGGACAAAGCCCTGCATGAGCCGGGCGACGCCATGAAGACCGCGAGTGATTGCCAGTAGAGTAAAGACAGCGAGAATAATGCCTGTCACCAGCGGGGGAAAATCAAAAGaaaa                                                                               >  2:714221/1‑140 (MQ=255)                                                           tACCCAGATTATCGCCATCAACGGGACAAAGCCCTGCATGAGCCGGGCGACGCCATGAAGACCGCGAGTGATTGCCAGTAGAGTAAAGACAGCGAGAATAATGCCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCa                                                                          >  1:780506/1‑140 (MQ=255)                                                               cAGATTATCGCCATCAACGGGACAAAGCCCTGCATGAGCCGGGCGACGCCATGAAGACCGCGAGTGATTGCCAGTAGAGTAAAGACAGCGAGAATAATGCCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGgc                                                                      <  2:659274/140‑1 (MQ=255)                                                                     aTCGCCATCAACGGGACAAAGCCCTGCATGAGCCGGGCGACGCCATGAAGACCGCGAGTGATTGCCAGTAGAGTAAAGACAGCGAGAATAACGCCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGc                                                                >  2:370201/1‑140 (MQ=255)                                                                       cGCCATCAACGGGACAAAGCCCTGCATGAGCCGGGCGACGCCATGAAGACCGCGAGTGATTGCCAGTAGAGTAAAGACAGCGAGAATAATGCCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCaa                                                              <  2:19270/140‑1 (MQ=255)                                                                            tCAACGGGACAAAGCCCTGCATGAGCCGGGCGACGCCATGAAGACCGCGAGTGATTGCCAGTAGAGTAAAGACAGCGAGAATAATGCCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCg                                                         <  2:485050/140‑1 (MQ=255)                                                                            tCAACGGGACAAAGCCCTGCATGAGCCGGGCGACGCCATGAAGACCGCGAGTGATTGCCAGTAGAGTAAAGACAGCGAGAATAATGCCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCg                                                         <  1:568618/140‑1 (MQ=255)                                                                              aaCGGGACAAAGCCCTGCATGAGCCGGGCGACGCCATGAAGACCGCGAGTGATTGCCAGTAGAGTAAAGACAGCGAGAATAATGCCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGtt                                                       <  1:667023/140‑1 (MQ=255)                                                                                cGGGACAAAGCCCTGCATGAGCCGGGCGACGCCATGAAGACCGCGAGTGATTGCCAGTAGAGTAAAGACAGCGAGAATAATGCCTGTCACCGGCGGGGGAGAATGAAAGGAAAAACTGAGGGTGCGGGCAACGGCGTTCg                                                     >  2:232130/1‑140 (MQ=255)                                                                                cGGGACAAAGCCCTGCATGAGCCGGGCGACGCCATGAAGACCGCGAGTGATTGCCAGTAGAGTAAAGACAGCGAGAATAATGCCTGTCACCAGCGGGGGAAAGTCAAAAGGAAAACTGAGGGCGGGGGCAACGGCGTTCg                                                     >  1:234683/1‑140 (MQ=255)                                                                                                    gcctggCGACGCCATGAAGACCGCGAGTGATTGCCAGTAGAGTAAAGACAGCGAGAATAATGCCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAAtt                                 >  1:789194/5‑140 (MQ=255)                                                                                                    gCCGGGCGACGCCATGAAGACCGCGAGTGATTGCCAGTAGAGTAAAGACAGCGAGAATAATGCCTGTCACCAGCGGGGGAGAATCAAAAGAAAAACTCAGGGCGCGGGCACCGGCGTACGCTTGGACTCCGCTGAAAAtt                                 >  1:279166/1‑140 (MQ=255)                                                                                                    gCCGGGCGACGCCATGAAGACCGCGAGTGATTGCCAGTAGAGTAAAGACAGCGAGAATAATGCCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAAtt                                 >  1:696624/1‑140 (MQ=255)                                                                                                    gCCGGGCGACGCCATGAAGACCGCGAGTGATTGCCAGTAGAGTAAAGACAGCGAGAATAATGCCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGAGCGAGGGAAACGGCGTCCGCTCGAACTCCGCTGAAAAtt                                 >  2:287583/1‑140 (MQ=255)                                                                                                       gggCGACGCCATGAAGACCGCGAGTGATTGCCAGTAGAGTAAAGACAGCGAGAATAATGCCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATg                              >  1:522036/1‑140 (MQ=255)                                                                                                                cATGAAGACCGCGAGTGCTTGCCAGTAGAGTTAAGTCAGCGAGAATAAGGCCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCg                     <  2:234683/140‑1 (MQ=255)                                                                                                                 aTGAAGACCGCGAGTGATTGCCGGTAGAGTATAGAAAGCTAGAATAATGCCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGa                    <  2:341924/140‑1 (MQ=255)                                                                                                                  tGAAGACCGCGAGTGATTGCCAGTAGAGTAAAGACAGCGAGAATAATGCCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGAt                   <  1:791939/140‑1 (MQ=255)                                                                                                                       aCCGCGAGTGATTGCCAGTAGGGTAAAGACAACGAGAATAATGCCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCa              >  1:729891/1‑140 (MQ=255)                                                                                                                              gTGATTGCCAGTAGAGTAAAGACAGCGAGAATAATGCCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAg         <  1:857342/138‑1 (MQ=255)                                                                                                                              gTGATTGCCAGTAGAGTAAAGACAGCGAGAATAATGCCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAg         >  2:857342/1‑138 (MQ=255)                                                                                                                                   tGCCAGTAGAGTAAAGACAGCGAGAATAATGCCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTTAAAATTAAGCCATACGCGATGCGCAAAAAGACGGCGa  >  1:381724/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                      ACCAAATGACGTGGGGAAGTTGCCCGATATTCATTACGCAAATTACCAGGCTGGTCAGTACCCAGATTATCGCCATCAACGGGACAAAGCCCTGCATGAGCCGGGCGACGCCATGAAGACCGCGAGTGATTGCCAGCAGAGTAAAGACAGCGAGAATAATGCCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCGA  >  NC_000913/7254‑7523

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑