| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,970,436 | T→C | I6T (ATA→ACA) | ygdQ → | UPF0053 inner membrane protein YgdQ |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,970,436 | 0 | T | C | 97.8% | 148.1 / NA | 45 | I6T (ATA→ACA) | ygdQ | UPF0053 inner membrane protein YgdQ |
| Reads supporting (aligned to +/- strand): ref base T (1/0); new base C (20/24); total (21/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.67e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CACCAGTGCACTACTGGCCCGTCATTTTCTGATCCAGTAGCCATCGCTTTGACCTGCCGCTTTCCGGGCATATAATTACCGCTTCATTTTTTTGGCAGGGCTTTTTAGATGTTATTTGCATGGATAACCGATCCTAACGCCTGGCTTGCGCTCGGTACGCTGACGCTGCTGGAGATCGTTCTTGGGATCGACAATATTATTTTCCTTTCTCTGGTGGTGGCAAAGCTTCCCACAGCACAACGTGCTCATGCGCGCCGTC > NC_000913/2970312‑2970570 | cACCAGTGCACTACTGGCCCGTCATTTTCTGATCCAGTAGCCATCGCTTTGACCTGCCGCTTTCCGGGCATATAATTACCGCTTCATTTTTTTGGCAGGGCTTTTTAGATGTTATTTGCATGGACAACCGATCCTAACGc > 1:793612/1‑140 (MQ=255) gTGCACTACTGGCCCGTCATTTTCTGATCCAGTAGCCATCGCTTTGACCTGCCGCTTTCCGGGCATATAATTACCGCTTCATTTTTTTGGCAGGGCTTTTTAGATGTTATTTGCATGGACAACCGATCCTAACGCCTGGc > 2:492562/1‑140 (MQ=255) cACTACTGGCCCGTCATTTTCTGATCCAGTAGCCATCGCTTTGACCTGCCGCTTTCCGGGCATATAATTACCGCTTCATTTTTTTGGCAGGGCTTTTTAGATGTTATTTGCATGGACAACCGATCCTAACGCCTGGCTTg > 1:771511/1‑140 (MQ=255) ctactGGCCCGTCATTTTCTGACCCAGTACCCATCGCATTGACCTGCCGCTTTCCGGGCATATAATTACCGCTTCATTTTTTTGGCAGGGCTTTTTAGATGTTATTTGCATGGACAACCGATCCTAACGCCTGGCTTgcg < 1:412386/140‑1 (MQ=255) actGGCCCGTCATTTTCTGATCCAGGAGCAATCGCTTGGACCTGCCGCTTTCCGGGCATATAATTACCGCTTCATTTTTTTGGCAGGGCTTTTTAGATGTTATTTGCATGGACAACCGATCCTAACGCCTGGCTTGCGCt < 1:413930/140‑1 (MQ=255) gCCCGTCATTTTCTGATCCAGTAGCCATCGCTTTGACCTGCCGCTTTCCGGGCATATAATTACCGCTTCATTTTTTTGGCAGGGCTTTTTAGATGTTATTTGCATGGACAACCGATCCTAACGCCTGGCTTGCGCt < 1:18103/136‑1 (MQ=255) gCCCGTCATTTTCTGATCCAGTAGCCATCGCTTTGACCTGCCGCTTTCCGGGCATATAATTACCGCTTCATTTTTTTGGCAGGGCTTTTTAGATGTTATTTGCATGGACAACCGATCCTAACGCCTGGCTTGCGCt > 2:18103/1‑136 (MQ=255) gCCCGTCATTTTCTGATCCAGTAGCCATCGCTTTGACCTGCCGCTTTCCGGGCATATAATTACCGCTTCATTTTTTTGGCAGGGCTTTTTAGATGTTATTTGCATGGACAACCGATCCTAACGCCTGGCTTGCGCTCGGt > 1:63483/1‑140 (MQ=255) gCCCGTCATTTTCTGATCCAGTAGCCATCGCTTTGACCTGCCGCTTTCCGGGCATATAATTACCGCTTCATTTTTTTGGCAGGGCTTTTTAAATGTTATTCGCATGGATAACCGATCCTAACACCTGGCTTCCGCTCGGt > 2:413930/1‑140 (MQ=255) tCATTTTCTGATCCAGTAGCCATCGCTTTGACCTGCCGCTTTCCGGGCATATAATTACCGCTTCATTTTTTTGGCAGGGCTTTTTAGATGTTATTTGCATGGACAACCGATCCTAACGCCTGGCTTGCGCTCGGTacgct > 2:136017/1‑140 (MQ=255) tCATTTTCTGATCCAGTAGCCATCGCTTTGACCTGCCGCTTTCCGGGCATATAATTACCGCTTCATTTTTTTGGCAGGGCTTTTTAGATGTTATTTGCATGGACAACCGAACCTGACACCTGGCGTGCGCTCGGTacgct > 1:256460/1‑140 (MQ=255) aTTTTCTGATCCAGTAGCCATCGCTTTGACCTGCCGCTTTCCGGGCATATAATTACCGCTTCATTTTTTTGGCAGGGCTTTTTAGATGTTATTTGCATGGACAACCGATCCTAACGCCTGGCTTGCGCTCGGTacgctga < 1:183500/140‑1 (MQ=255) aTTTTCTGATCCAGTAGCCATCACTATGATCTGCCGCTTTCCGGGCATATAATTACCGCTTCATTTTTTTGGCAGGGCTTTTTAGATGTTATTTGCATGGACAACCGATCCTAACGCCTGGCTTGCGCTCGGTacgctga < 1:409617/140‑1 (MQ=255) tttCTGATCCAGTAGTCATCGCTTTGACCGGCCGCTTTCCGGGCATATAATTACCGCTTCATTTTTTTGGCAGGGCTTTTTAGATGTTATTTGCATGGACAACCGATCCTAACGCCTGGCTTGCGCTCGGTacgctgacg < 1:360884/140‑1 (MQ=255) tttCTGATCCAGTAGCCATCGCTTTGACCTGCCGCTTTCCGGGCATATAATTACCGCTTCATTTTTTTGGCAGGGCTTTTTAGATGTTATTTGCATGGACAACCGATCCTAACGCCTGGCTTGCGCTCGGTacgctgacg < 1:136017/140‑1 (MQ=255) tttCTGATCCAGTAGCCATCGCCTTTACCTGCCGCTTCCCGGGCATATAATTACCGCTTCATTTTTTTGGCAGGGCTTTTTAGATGTTATTTGCATGGACAACCGATCCTAACGCCTGGCTTGCGCTCGGTacgctgacg < 1:306993/140‑1 (MQ=255) tCCAGTAGCCATCGCTTTGACCTGCCGCTTTCCGGGCATATAATTACCGCTTCATTTTTTTGGCAGGGCTTTTTAGATGTTATTTGCATGGACAACCGATCCTAACGCCTGGCTTGCGCTCGGTACGCTGACGCTGCTgg > 1:21574/1‑140 (MQ=255) tAGCCATCGCTTTGACCTGCCGCTTTCCGGGCATATAATTACCGCTTCATTTTTTTGGCAGGGCTTTTTAGATGTTATTTGCATGGACAACCGATCCTAACGCCTGGCTTGCGCTCGGTACGCTGACGCTGCTGGAGATc > 2:863161/1‑140 (MQ=255) cccTTTGACCTGCCGCTCTCCGCGCATATAATTACCGCTCCATTTTTTCGGCAGGGCTTTTTAGATGTTATTTGCATGGACAACCGATCCTAACGCCTGGCTTGCGCTCGGTACGCTGACGCTGCTGGAGATCGTTCTTg < 2:256460/138‑1 (MQ=255) cTTTGACCTGCCGCTTTCCGGGCATATAATTACCGCTTCATTTTTTTGGCAGGGCTTTTTAGATGTTATTTGCATGGACAACCGATCCTAACGCCTGGCTTGCGCTCGGTACGCTGACGCTGCTGGAGATCGTTCTTggg < 1:492562/140‑1 (MQ=255) tGCCGCTTTCCGGGCATATAATTACGGCTTCATTTTTGTGGCAGGGCTTTTTAGATGTTATTTGCATGGACAACCGATCCTAACGCCTGGCTTGCGCTCGGTACGCTGACGCTGCTGGAGATCGTTCTTGGGATCGACaa < 1:298843/140‑1 (MQ=255) gCCGCTTTCCGGGCATATAATTACCGCTTCATTTTTTTGGCAGGGCTTTTTAGATGTTATTTGCATGGACAACCGATCCTAACGCCTGGCTTGCGCTCGGTACGCTGACGCTGCTGGAGATCGTTCTTGGGATCGa > 1:616189/1‑136 (MQ=255) gCCGCTTTCCGGGCATATAATTACCGCTTCATTTTTTTGGCAGGGCTTTTTAGATGTTATTTGCATGGACAACCGATCCTAACGCCTGGCTTGCGCTCGGTACGCTGACGCTGCTGGAGATCGTTCTTGGGATCGa < 2:616189/136‑1 (MQ=255) ccGCTTTCCGGGCATATAATTACCGCTTCATTTTTTTGGCAGGGCTTTTTAGATGTTATTTGCATGGACAACCGATCCTAACGCCTGGCTTGCGCTCGGTACGCTGACGCTGCTGGAGATCGTTCTTGGGATCGACAata < 1:863161/140‑1 (MQ=255) gCTTTCCGGGCATATAATTACCGCTTCATTTTTTTGGCAGGGCTTTTTAGATGTTATTTGCATGGACAACCGATCCTAACGCCTGGCTTGCGCTCGGTACGCTGACGCTGCTGGAGATCGTTCTTGGGATCGACAAtatt < 2:793612/140‑1 (MQ=255) ccGGGCATATAATTACCGCTTCATTTTTTTGGCAGGGCTTTTTAGATGTTATTTGCATGGACAACCGATCCTAACGCCTGGCTTGCGCTCGGTACGCTGACGCTGCTGGAGATCGTTCTTGGGATCGACAATATTAtttt < 1:729781/140‑1 (MQ=255) ggCATATAATTACCGCTTCATTTTTTTGGCAGGGCTTTTTAGATGTTATTTGCATGGACAACCGATCCTAACGCCTGGCTTGCGCTCGGTACGCTGACGCTGCTGGAGATCGTTCTTGGGATCGACAATATTATTTTCCt < 2:771511/140‑1 (MQ=255) ataATTACCGCTTCATTTTTTTGGCAGGGCTTTTTAGATGTTATTTGCATGGACAACCGATCCTAACGCCTGGCTTGCGCTCGGTACGCTGACGCTGCTGGAGATCGTTCGTGGGATCGACAATATTATTTTCCTTtctc > 1:324161/1‑140 (MQ=255) ttACCGCTTCATTTTTTTGGCATGGTTTTGTAGATGTTATTTGCATGGACAACCGATCCTAACGCCTGGCTTGCGCTCGGTACGCTGACGCTGCTGGAGATCGTTCTTGGGATCGACAATATTATTTTCCTTTCTCtggt < 1:281590/140‑1 (MQ=255) aCCGCTTCATTTTTTTGGCAGGGCTTTTTAGATGTTATTTGCATGGACAACCG‑TCCTAACGCCTGGCTTGCGCTCGGTACGCTGACGCTGCTGGAGATCGTTCTTGGGATCGACAATATTATTTTCCTTTCTCtggtggt > 1:11548/1‑140 (MQ=255) cGCTTCATTTTTTTGGCAGGGCTTTTTAGATGTTATTTGCATGGACAACCGATCCTAACGCCTGGCTTGCGCTCGGTACGCTGACGCTGCTGGAGATCGTTCTTGGGATCGACAATATTATTTTCCTTTCTCtggtggtg > 1:47791/1‑140 (MQ=255) cTTCATTTTTTTGGCAGGGCTTTTTAGATGTTATTTGCATGGACAACCGATCCTAACGCCTGGCTTGCGCTCGGTACGCTGACGCTGCTGGTGATCGTTCATGGGATCCACAATATTATTTTGCTTTCTCTGGTGGTGGc > 2:255909/1‑140 (MQ=255) cTTCATTTTTTTGGCAGGGCTTTTTAGATGTTATTTGCATGGACAACCGATCCTAACGCCTGGCTTGCGCTCGGTACGCTGACGCTGCTGGAGCTCGTTCTTGGGATCGAAAATATTGTTTTCCGTTCTCTGGTGGTGGc > 1:413972/1‑140 (MQ=255) cTTCATTTTTTTGGCAGGGCTTTTTAGATGTTATTTGCATGGACAACCGATCCTAACGCCTGGCTTGCGCTCGGTACGCTGACGCTGCTGGAGATCGTTCTTGGGATCGACAATATTATTTTCCTTTCTCTGGTGGTGGc > 1:198177/1‑140 (MQ=255) ttCATTTTTTTGGCAGGGCTTTTTAGATGTTATTTGCATGGACATCCGATCCTAACGCCTGGCTTGCGCTCGGTACGCTGACGCTGCTGGAGATCGTTCTTGGGATCGACAATATTATTTTCCTTTCTCTGGTGGTGGCa < 2:640931/140‑1 (MQ=255) tCATTTTTTTGGCAGGGCTTTTTAGATGTTATTTGCATGGACAACCGATCCTAACGCCTGGCTTGCGCTCGGTACGCTGACGCTGCTGGAGATCGTTCTTGGGATCGACAATATTATTTTCCTTTCTCTGGTGGTGGCaa > 2:829182/1‑140 (MQ=255) tttGGCAGGGCTTTTCAGATGTCATATGCCTGGACAACCGATCCTAACGCCTGGCTTGCGCTCGGTACGCTGACGCTGCTGGAGATCGTTCTTGGGATCGACAATATTATTTTCCTTTCTCTGGTGGTGGCAAAGCTTcc < 1:255909/140‑1 (MQ=255) tttGGCAGCGCTTTTTGCATGTTATTTGCAAAGTCTACCTATCCTAACACCTGGCTTGCGCTCGGTACGCTGACGCTGCTGGAGATCGTTCTTGGGATCGACAATATTATTTTCCTTTCTCTGGTGGTGGCAAAGCTTcc < 2:413972/140‑1 (MQ=255) cAGGGCTTTTTAGATGTTATTTCCATGGACATCCGATCCAAACGCCTGGCTTGCGCTCGGTACGCTGACGCTGCTGGAGATCGTTCTTGGGATCGACAATATTATTTTCCTTTCTCTGGTGGTGGCAAAGCTTCCCACAg < 2:324161/140‑1 (MQ=255) aGGGCTTTTTAGATGTTATTTGCATGGACAACCGATCCTAACGCCTGGCTTGCGCTCGGTACGCTGACGCTGCTGGAGATCGTTCTTGGGATCGACAATATTATTTTCCTTTCTCTGGTGGTGGCAAAGCTTCCCACAGc > 1:633750/1‑140 (MQ=255) gggCTTTTTAGATGTTATTTGCATGGACAACCGATCCTAACGCCTGGCTTGCGCTCGGTACGCTGACGCTGCTGGAGATCGTTCTTGGGATCGACAATATTATTTTCCTTTCTCTGGTGGTGGCAAAGCTTCCCACAGca < 1:624334/140‑1 (MQ=255) gCTTTTTAGATGTTATTTGCATGGACAACCGATCCTAACGCCTGGCTTGCGCTCGGTACGCTGACGCTGCTGGAGATCGTTCTTGGGATCGACAATATTATTTTCCTTTCTCTGGTGGTGGCAAAGCTTCCCACAGcaca < 1:819026/140‑1 (MQ=255) tGTTATTTGCATGGACAACCGATCCTAACGCCTGGCTTGCGCTCGGTACGCTGACGCTGCTGGAGATCGTTCTTGGGATCGACAATATTATTTTCCTTTCTCTGGTGGTGGCAAAGCTTCCCACAGCACAACGTGCTCAt > 2:481008/1‑140 (MQ=255) gTTATTTGCATGGACAACCGATCCTAACGCCTGGCTTGCGCTCGGTACGCTGACGCTGCTGGAGATCGTTCTTGGGATCGACAATATTATTTTCCTTTCTCTGGTGGTGGCAAAGCTTCCCACAGCACAACGTGCTCATg > 1:537827/1‑140 (MQ=255) tttGCATGGACAACCGATCCTAACGCCTGGCTTGCGCTCGGTACGCTGACGCTGCTGGAGATCGTTCTTGGGATCGACAATATTATTTTCCTTTCTCTGGTGGTGGCAAAGCTTCCCACAGCACAACGTGCTCATgcgcg < 1:481008/140‑1 (MQ=255) aTGGACAACCGATCCTAACGCCTGGCTTGCGCTCGGTACGCTGACGCTGCTGGAGATCGTTCTTGGGATCGACAATATTATTTTCCTTTCTCTGGTGGTGGCAAAGCTTCCCACAGCACAACGTGCTCATGCGCGCCGTc < 2:198177/140‑1 (MQ=255) | CACCAGTGCACTACTGGCCCGTCATTTTCTGATCCAGTAGCCATCGCTTTGACCTGCCGCTTTCCGGGCATATAATTACCGCTTCATTTTTTTGGCAGGGCTTTTTAGATGTTATTTGCATGGATAACCGATCCTAACGCCTGGCTTGCGCTCGGTACGCTGACGCTGCTGGAGATCGTTCTTGGGATCGACAATATTATTTTCCTTTCTCTGGTGGTGGCAAAGCTTCCCACAGCACAACGTGCTCATGCGCGCCGTC > NC_000913/2970312‑2970570 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |