Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,973,182	T→C	L227L (TTA→TTG)	lplT ←	lysophospholipid transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,973,182	0	T	C	100.0%	124.8 / NA	36	L227L (TTA→TTG)	lplT	lysophospholipid transporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (19/17);  total (19/17)

CGCCAACCACGATACCAATCGCTACCATCGCGTTGAGATAGGTGGGCGTAGCGTTATCGGTAATGCCCAGCGCCACCGGTACCCACAGCACCAACAGGAAACGCAGCGTGACACCCGCTCCCCAGAATAAACTGGTGCCCACCAGCGAAAAACGCGTTTCACCATTGCGCCATAGCGAGGTGCAGGCATTCAGGAAACTGCGGGTCATGTTGATGAGATTCCAGGACTGCCCCGGACGCGCCGCCGCCAGTTTGGGAATG  >  NC_000913/2973055‑2973314                                                                                                                                |                                                                                                                                     cgcCAACCACGATACCAATCGCTACCATCGCGTTGAGATAGGTGGGCGTAGCGTTATCGGTAATGCCCAGCGCCACCGGTACCCACAGCACCAACAGGAAACGCAGCGTGACACCCGCTCCCCAGAACAAACTGGTGccc                                                                                                                          >  1:537042/1‑140 (MQ=255)     aaCCACGATACCAATCGCTACCATCGCGTTGAGATAGGTGGGCGTAGCGTTATCGGTAATGCCCAGCGCCACCGGTACCCACAGCACCAACAGGAAACGCAGCGTGACACCCGCTCCCCAGAACAAACTGGTGCccacca                                                                                                                      >  1:754885/1‑140 (MQ=255)             tACCAATCGCTACCATCGCGTTGAGATAGGTGGGCGTAGCGTTATCGGTAATGCCCAGCGCCACCGGTACCCACAGCACCAACAGGAAACGCAGCGTGACACCCGCTCCCCAGAACAAACTGGTGCCCACCAGCGaaaaa                                                                                                              <  2:754885/140‑1 (MQ=255)                  aTCGCTACCATCGCGTTGAGATAGGTGGGCGTAGCGTTATCGGTAATGCCCAGCGCCACCGGTACCCACAGCACCAACAGGAAACGCAGCGTGACACCCGCTCCCCAGAACAAACTGGTGCCCACCAGCGAAAAACGCGt                                                                                                         >  1:470982/1‑140 (MQ=255)                       tACCATCGCGTTGAGATAGGTGGGCGTAGCGTTATCGGTAATGCCCAGCGCCACCGGTACCCACAGCACCAACAGGAAACGCAGCGTGACACCCGCTCCCCAGAACAAACTGGTGCCCACCAGCGAAAAACGCGTTTCAc                                                                                                    >  2:8433/1‑140 (MQ=255)                         ccATCGCGTTGAGATAGGTGGGCGTAGCGTTATCGGTAATGCCCAGCGCCACCGGTACCCACAGCACCAACAGGAAACGCAGCGTGACACCCGCTCCCCAGAACAAACTGGTGCCCACCAGCGAAAAACGCGTTTCACCa                                                                                                  <  2:470982/140‑1 (MQ=255)                            tCGCGTTGAGATAGGTGGGCGTAGCGTTATCGGTAATGCCCAGCGCCACCGGTACCCACAGCACCAACAGGAAACGCAGCGTGACACCCGCTCCCCAGAACAAACTGGTGCCCACCAGCGAAAAACGCGTTTCACCATTg                                                                                               <  2:514421/140‑1 (MQ=255)                             cgcgTTGAGATAGGTGGGCGTAGCGTTATCGGTAATGCCCAGCGCCACCGGTACCCACAGCACCAACAGGAAACGCAGCGTGACACCCGCTCCCCAGAACAAACTGGTGCCCACTAGCGAAAAATGCGTTTCACCATTgc                                                                                              >  1:404200/1‑140 (MQ=255)                             cgcgTTGAGATAGGTGGGCGTAGCGTTATCGGTAATGCCCAGCGCCACCGGTACCCACAGCACCAACAGGAAACGCAGCGTGACACCCGCTCCCCAGAACAAACTGGTGCCCACCAGCGAAAAACGCGTTTCACCATTgc                                                                                              >  1:587691/1‑140 (MQ=255)                             cgcgTTGAGATAGGTGGGCGTAGCGTTATCGGTAATGCCCAGCGCCACCGGTACCCACAACACCAACAGGAAACGCAGCGTGACACCCGCTCCCCAGAACAAACTGGTGCCCACCAGCGAAAAACGCGTTTCACCATTgc                                                                                              >  1:844328/1‑140 (MQ=255)                             cgcgTTGAGATAGGTGGACGTAGCGTTATCGGTAATGCCCAGCGCCACCGGTACCCACAGCACCAACAGGAAACGCAGCGTGACACCCGCTCCCCAGAACAAACTGGTGCCCACCAGCGAAAAACGCGTTTCACCATTgc                                                                                              >  1:101354/1‑140 (MQ=255)                                   gagaTAGGTGGGCGTAGCGTTATCGGTAATGCCCAGCGCCACCGGTACCCACAGCACCAACAGGAAACGCAGCGTGACACCCGCTCCCCAGAACAAACTGGTGCCCACCACCGAGAAACGCGTTTCACCATTGCGCCATa                                                                                        >  1:385998/1‑140 (MQ=255)                                         ggTGGGCGTAGCGTTATGGGTACTGCCCAGTCCGAGCGGCACCCACAGCACCAACAGGAAACGCAGCGTGACACCCGCTCCCCAGAACAAACTGGTGCCCACCAGCGAAAAACGCGTTTCACCATTGCGCCATAGCGAgg                                                                                  <  2:404200/140‑1 (MQ=255)                                         ggTGGGCGTAGCGTTATCGGTAATGCCCAGCGCCACCGGTACCCACAGCACCAACAGGAAACGCAGCGTGACACCCGCTCCCCAGAACAAACTGGTGCCCACCAGCGAAAAACGCGTTTCACCATTGCGCCATAGCGAgg                                                                                  <  2:704926/140‑1 (MQ=255)                                                gTAGCGTTATCGGTAATGCCCAGTGCCACCGGTACCCACAGCACCAACAGGAAACGCAGCGTGACACCCGCTCCCCAGAACAAACTGGTGCCCACCAGCGAAAAACGCGTTTCACCATTGCGCCATAGCGAGGTGCAGGc                                                                           >  1:666373/1‑140 (MQ=255)                                                    cGTTATCGGTAATGCCCAGCGCCACCGGTACCCACAGCACCAACAGGAAACGCAGCGTGACACCCGCTCCCCAGAACAAACTGGTGCCCACCAGCGAAAAACGCGTTTCACCATTGCGCCATAGCGAGGTGCAGGCATTc                                                                       >  2:462163/1‑140 (MQ=255)                                                        aTCGGTAATGCCCAGCGCCACCGGTACCCACAGCACCAACAGGAAACGCAGCGTGACACCCGCTCCCCAGAACAAACTGGTGCCCACCAGCGAAAAACGCGTTTCACCATTGCGCCATAGCGAGGTGCAGGCATTCAGGa                                                                   >  2:22976/1‑140 (MQ=255)                                                         tCGGTAATGCCCAGCGCCACCGGTACCCACAGCACCAACAGGAAACGCAGCGTGACACCCGCTCCCCAGAACAAACTGGTGCCCACCAGCGAAAAACGCGTTTCGCCATTGCGCCATAGCGAGGTGCAGGCATTCAGGaa                                                                  <  1:633886/140‑1 (MQ=255)                                                                   ccAGCGCCACCGGTACCCACAGCACCAACAGGAAACGCAGCGTGACACCCGCTCCCCAGAACAAACTGGTGCCCACCAGCGAAAAACGCGTTTCACCATTGCTCCATAGCGAGGCGCGGGCATTCAGGAAAATGCGGGTc                                                        >  1:383174/1‑140 (MQ=255)                                                                   ccAGCGCCACCGGTACCCACAGCACCAACAGGAAACGCAGCGTGACACCCGCTCCCCAGAACAAACTGGTGCCCACCAGCGAAAAACGCGTTTCACCATTGCGCCATAGCGAGGTGCAGGCATTCAGGAAACTGCGGGTc                                                        <  2:587691/140‑1 (MQ=255)                                                                    cAGCGCCACCGGTACCCACAACACCAACAGGAAACGCAGCGTGACACCCGCTCCCCAGAACAAACTGGTGCCCACCAGCGAAAAACGCGTTTCACCATTGCGCCATAGCGAGGTGCAGGCATTCAGGAAACTGCGGGTCa                                                       <  2:844328/140‑1 (MQ=255)                                                                       cgcCACCGGTACCCACAGCACCAACAGGAAACGCAGCGTGACACCCGCTCCCCAGAACAAACTGGTGCCCACCAGCGAAAAACGCGTTTCACCATTGCGCCATAGCGAGGTGCAGGCTTTCAGGTAACTGCGGGTCATGt                                                    >  1:388913/1‑140 (MQ=255)                                                                       cgcCACCGGTACCCACAGCACCAACAGGAAACGCAGCGTGACACCCGCTCCCCAGAACAAACTGGTGCCCACCAGCGAAAAACGCGTTTCACCATTGCGCCATAGCGAGGTGCAGGCATTCAGGAAACTGCGGGTCATGt                                                    >  2:718210/1‑140 (MQ=255)                                                                           aCCGGTACCCACAGCACCAACAGGAAACGCAGCGTGACACCCGCTCCCCAGAACAAACTGGTGCCCACCAGCGAAAAACGCGTTTCACCATTGCGCCATAGCGAGGTGCAGGCATTCAGGAAACTGCGGGTCATGTtgat                                                <  2:44192/140‑1 (MQ=255)                                                                             cGGTACCCACAGCACCAACAGGAAACGCAGCGTGACACCCGCTCCCCAGAACAAACTGGTGCCCACCAGCGAAAAACGCGTTTCACCATTGCGCCATAGCGAGGTGCAGGCATTCAGGAAACTGCGGGTCATGTtgatga                                              <  1:8433/140‑1 (MQ=255)                                                                             cGGTACCCACAGCACCAACAGGAAACGCAGCGTGACACCCGCTCCCCAGAACAAACTGGTGCCCACCAGCGAAAAACGCGTTTCACCATTGCGCCATAGCGAGGTGCAGGCATTCAGGAAACTGCGGGTCATGTtgatga                                              <  2:666373/140‑1 (MQ=255)                                                                                       aGCACCAACAGGAAACGCAGCGTGACACCCGCTCCCCAGAACAAACTGGTGCCCACCAGCGAAAAACGCGTTTCACCATTGCGCCATAGCGTGGTGCAGGTATTAAGGAAACTGCGGGTCATAATGATGAGATTCCAGGa                                    >  2:424233/1‑140 (MQ=255)                                                                                       aGCACCAACAGGAAACGCAGCGTGACACCCGCTCCCCAGAACAAACTGGTGCCCACCAGCGAAAAACGCGTTTCACCATTGCGCCATAGCGAGGTGCAGGCATTCAGGAAACTGGGGCTCATGTTGATGAGATTCCAGGa                                    >  1:382612/1‑140 (MQ=255)                                                                                       aGCACCAACAGGAAACGCAGCGTGACACCCGCTCCCCAGAACAAACTGGTGCCCACCAGCGAAAAACGCGTTTCACCATTGCGCCATAGCGAGGTGCAGGCATTCAGGAAACTGCGGGTCATGTTGATGAGATTCCAGGa                                    <  1:132556/140‑1 (MQ=255)                                                                                       aGCACCAACAGGAAACGCAGCGGGACACCCGCTCCTCAGAACAAACTGCTGCCCACCAGCGAAAAACGCGTTTCACCATTGCGCCATAGCGAGGTGCAGGCATTCAGGAAACTGCGGGTCATGTTGATGAGATTCCAGGa                                    <  2:327199/140‑1 (MQ=255)                                                                                              aCAGGAAACGCAGCGTGACACCCGCTCCCCAGAACAAACTGGTGCCCACCAGCGAAAAACGCGTTTCACCATTGCGCCATAGCGAGGTGCAGGCATTCAGGAAACTGCGGATCACGTAGATGAGCTTCCAGGACTGcccc                             >  1:327199/1‑140 (MQ=255)                                                                                                    aaCGCAGCGTGACACCCGCTCCCCAGAACAAACTGGTGCCCACCAGCGAAAAACGCGTTTCACCATTGCGCCATAGCGAGGTGCAGGCATTCAGGAAACTGCGGGTCATGTTGATGAGATTCCAGGACTGCCCCGGAcgc                       <  2:76614/140‑1 (MQ=255)                                                                                                     aCGCAGCGTGACACCCGCTCCCGAGAACAGACTGGTGTCCACCAGCGAAAAACGCGTTTCACCATTGCGCCATAGCGAGGTGCAGGCATTCAGGAAACTGCGGGTCATGTTGATGAGATTCCAGGACTGCCCCGGAcgcg                      <  1:424233/140‑1 (MQ=255)                                                                                                         aGCGTGACACCCGCTCCCCAGAACAAACTGGTGCCCACCAGCGAAAAACGCGTTTCACCATTGCGCCATAGCGAGGTGCAGGCATTCAGGATACTGCGGGTCATGTGGACGAGATTCGAGGACTGCCCCGGACGcgccgc                  >  2:411489/1‑140 (MQ=255)                                                                                                                    cGCTCCCCAGAACAAACTGGTGCCCACCAGCGAAAAACGCGTTTCACCATTGCGCCATAGCGAGGTGCAGGCATTCAGGAAACTGCGGGTCATGTTGATGAGATTCCAGGACTGCCCCGGACGCGCCGCCGCCAGTTTgg       <  2:537042/140‑1 (MQ=255)                                                                                                                         cccAGAACAAACTGGTGCCCACGAGCGAAAATCGCCTTTCACCATTGCGCCATAGCGAGGTGCAGGCATTCAGGAAACTGCGGGTCATGTTGATGAGATTCCAGGACTGCCCCGGACGCGCCGCCGCCAGTTTGGGAATg  <  2:347544/140‑1 (MQ=255)                                                                                                                                |                                                                                                                                     CGCCAACCACGATACCAATCGCTACCATCGCGTTGAGATAGGTGGGCGTAGCGTTATCGGTAATGCCCAGCGCCACCGGTACCCACAGCACCAACAGGAAACGCAGCGTGACACCCGCTCCCCAGAATAAACTGGTGCCCACCAGCGAAAAACGCGTTTCACCATTGCGCCATAGCGAGGTGCAGGCATTCAGGAAACTGCGGGTCATGTTGATGAGATTCCAGGACTGCCCCGGACGCGCCGCCGCCAGTTTGGGAATG  >  NC_000913/2973055‑2973314

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑