Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,973,904	A→G	F704S (TTT→TCT)	aas ←	acyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,973,904	0	A	G	100.0%	133.4 / NA	39	F704S (TTT→TCT)	aas	acyltransferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (18/21);  total (18/21)

TATCGCCAAACGCAGAGAGAAACTGCGCCACGATAACCGCTTTCATCCCCTTCGACCACAACGAAGTGTTAGTGTGCACTGACTCACTCATCGTGTTGTTCCGCTTCGTCTACCCAGCTTTTCAACGTGACAAAGTCAGGTTTGCCGCTGCCAAGTAATGGCATCTGTTTCAGATAGCGAATATCGCGCGGTACAGCAAGCTCCGGCACGCCGTGCTCGCGGGCATACTGTTGCAACTTATCGCGCGT  >  NC_000913/2973772‑2974019                                                                                                                                     |                                                                                                                    tATCGCCAAACGCAGAGAGAAACTGCGCCACGATAACCGCTTTCATCCCCTTCGACCACAACGAAGTGTTAGTGTGCACTGACTCACTCATCGTGTTGTTGCGCATCGGCTACCCAGATTTTCAACGTGACAGAGTCAgg                                                                                                              >  2:311490/1‑140 (MQ=255)        aaaCGCAGAGAGAAACTGCGCCACGATAACCGCTTTCATCCCCTTCGACCACAACGAAGTGTTAGTGTGCACTGACTCACTCATCGTGTTGTTCCGCTTCGTCTACCCAGCTTTTCAACGTGACAGAGTCAGGTTTGCCg                                                                                                       <  1:162011/140‑1 (MQ=255)         aaCGCAGAGAGAAACTGCGCCACGATAACCGCTTTCATCCCCTTCGACCACAACGAAGTGTTAGTGTGCACTGACTCACTCATCGTGTTGTTCCGCTTCGTCTACCCAGCTTTTCAACGTGACAGAGTCAGGTTTGCCGc                                                                                                      <  1:788677/140‑1 (MQ=255)             cAGAGAGAAACTGCGCCACGATAACCGCTTTCATCTCCTCCGACCACAACGAAGTGTTAGTGTGCACTGACTCACTCATCGTGTTGTTCCGCTTCGTCTACCCAGCTTTTCAACGTGACAGAGTCAGGTTTGCCGCTGcc                                                                                                  <  2:295311/140‑1 (MQ=255)               gagagaAACTGCGCCACGATAACCGCTTTCATCCCCTTCGACCACAACGAAGTGTTAGTGTGCACTGACTCACTCATCGTGTTGTTCCGCTTCGTCTACCCAGCTTTTCAACGTGACAGAGTCAGGTTTGCCGCTGCCaa                                                                                                <  1:757261/140‑1 (MQ=255)                    aaaCTGCGCCACGATAACCGCTTTCATCCCCTTCGACCACAACGAAGTGTTAGTGTGCACTGACTCACTCATCGTGTTGTTCCGCTTCGTCTACCCAGCTTTTCAACGTGACAGAGTCAGGTTTGCCGCTGCCAAGTAAt                                                                                           <  1:822111/140‑1 (MQ=255)                        tGCGCCACGATAACCGCTTTCATCCCCTTCGACCACAACGAAGTGTTAGTGTGCACTGACTCACTCATCGTGTTGTTCCGCTTCGTCTACCCAGCTTTTCAACGTGACAGAGTCAGGTTTGCCGCTGCCAAGTAATGGCa                                                                                       >  2:486962/1‑140 (MQ=255)                         gcgcCACGATAACCGCTTTCATTCCCTTCGACCACTACGGAGTGTTAGTGTGCACTGACTCACTCATCGTGTTGTTCCGCTTCGTCTACCCAGCTTTTCAACGTGACAGAGTCAGGTTTGCCGCTGCCAAGTAATGGCAt                                                                                      <  2:365761/140‑1 (MQ=255)                         gcgcCACGATAACCGCTTTCATCCCCTTCGACCACAACGAAGTGTTAGTGTGCACTGACTCACTCATCGTGTTGTTCCGCTTCGTCTACCCAGCTTTTCAACGTGACAGAGTCAGGTTTGCCGCTGCCAAGTAATGGCAt                                                                                      <  1:675675/140‑1 (MQ=255)                          cgcCACGATAACCGCTTTCATCCCCTTCGACCACAACGAAGTGTTAGTGTGCACTGACTCACTCATCGTGTTGTTCCGCTTCGTCTACCCAGCTTTTCAACGTGACAGAGTCAGGTTTGCCGCTGCCAAGTAATGGCATc                                                                                     >  2:433297/1‑140 (MQ=255)                          cgcCACGATAACCGCTTTCATCCCCTTCGACCACAACGAAGTGTTAGTGTGCACTGACTCACTCATCGTGTTGTTCCGCTTCGTCTACCCAGCTTTTCAACGGGACAGAGTCAGATTAGCCGCTACCAAGTAATGGCATc                                                                                     >  1:394359/1‑140 (MQ=255)                          cgcCACGATAACCGCTTTCATCCCCTTCGACCACAACGAAGTGTTAGTGTGCACTGACTCACTCATCGTGTTGTTCCGCTTCGTCTACCCAACTTTTCAAAGTGCCAGTGTCAGGTTGGCCGTTGCCAAGTAATGGCATc                                                                                     >  2:276585/1‑140 (MQ=255)                            ccACGATAACCGCTTTCATCCCCTTCGACCACAACGAAGTGTTAGTGTGCACTGACTCACTCATCGTGTTGTTCCGCTTCGTCTACCCAGCTTTTCAACGTGACAGAGTCAGGTTTGCCGCTGCCAAGTAATGGCATCTg                                                                                   >  2:160024/1‑140 (MQ=255)                               cGATAACCGCTTTCATCCCCTTCGACCACAACGAAGTGTTAGTGTGCACTGACTCACTCATCGTGTTGTTCCGCTTCGTCTACCCAGCTTTTCAACGTGACAGAGTCAGGTTTGCCGCTGCCAAGTAATGGCATCTGttt                                                                                <  1:762924/140‑1 (MQ=255)                                 aTAACCGCTTTCATCCCCTTCGACCACAACGAAGTGTTAGTGTGCACTGACTCACTCATCGTGTTGTTCCGCTTCGTCTACCCAGCTTTTCAACGTGACAGAGTCAGGTTTGCCGCTGCCAAGTAATGGc                                                                                        >  1:701537/1‑130 (MQ=255)                                 aTAACCGCTTTCATCCCCTTCGACCACAACGAAGTGTTAGTGTGCACTGACTCACTCATCGTGTTGTTCCGCTTCGTCTACCCAGCTTTTCAACGTGACAGAGTCAGGTTTGCCGCTGCCAAGTAATGGc                                                                                        <  2:701537/130‑1 (MQ=255)                                      cGCTTTCATCCCCTTCGACCACAACGAAGTGTTAGTGTGCACTGACTCACTCATCGTGTTGTTCCGCTTCGTCTACCCAGCTTTTCAACGTGACAGAGTCAGGTTTGCCGCTGCCAAGTAATGGCATCTGTTTCAGATAg                                                                         <  1:160024/140‑1 (MQ=255)                                          ttCATCCCCTTCGACCACAACGAAGTGTTAGTGTGCACTGACTCACTCATCGTGTTGTTCCGCTTCGTCTACCCAGCTTTTCAACGTGACAGAGTCAGGTTTGCCGCTGCCAAGTAATGGCATCTGTTTCAGATAGCGaa                                                                     >  2:781337/1‑140 (MQ=255)                                          ttCATCCCCTTCGACCACAACGAAGTGTTAGTGTGCACTGACTCACTCATCGTGTTGTTCCGCTTCGTCTACCCAGCTTTTCAACGTGACAGAGTCAGGTTTGCCGCTGCCAAGTAATGGCATCTGTTTCAGATAGCGaa                                                                     <  1:486962/140‑1 (MQ=255)                                          ttCATCCCCTTCGACCACAACGAAGTGTTAGTGTGCACTGACTCACTCATCGTGTTGTTCCGCTTCGTCTACCCAGCTTTTCAACGTGACAGAGTCAGGTTTGCCGCTGCCAAGTAATGGCATCTGTTTCAGATAGCGaa                                                                     <  2:268/140‑1 (MQ=255)                                           tCATCCCCTTCGACCACAACGAAGTGTTAGTGTGCACTGACTCACTCATCGTGTTGTTCCGCTTCGTCTACCCAGCTTTTCAACGTGACAGAGTCAGGTTTGGCGCTGCCGAGTGATGGCATCTATTTCAGATAGCGAat                                                                    >  1:249120/1‑140 (MQ=255)                                            cATCCCCTTCGACCACAACGAAGTGTTAGTGTGCACTGACTCACTCATCGTGTTGTTCCGCTTCGTCTACCCAGCTTTTCAACGTGACAGAGTCAGGTTTGCCGCTGCCAAGTAATGGCATCTGTTTCAGATAGCGAata                                                                   <  1:667923/140‑1 (MQ=255)                                                cccTTCGACCACAACCAAGTGTTAGTGTGTACTGACTCACTCATCGTGTTGTTCCGCTTCGTCTACCCAGCTTTTCAACGTGACAGAGTCAGGTTTGCCGCTGCCAAGTAATGGCATCTGTTTCAGATAGCGAATATcgc                                                               <  1:276585/140‑1 (MQ=255)                                                 ccTTCGACCACAACGAAGTGTTTGTGTGCACTGCCTCACCCATCGTGTTGTTCCGCTTCGTCTACCCAGCTTTTCAACGTGACAGAGTCAGGTTTGCCGCTGCCAAGTAATGGCATCTGTTTCAGATAGCGAATATcgcg                                                              <  2:394359/140‑1 (MQ=255)                                                   ttCGACCACAACGAAGTTTTAGCGTGCACTGCCTCCCTCCTCGTGTTGGTCCGCTTCGTCTACCCAGCTTTTCAACGTGACAGAGTCAGGTTTGCCGCTGCCAAGTAATGGCATCTGTTTCAGATAGCGAATATcgcgcg                                                            <  2:254094/140‑1 (MQ=255)                                                                   tgtTAGTGTGCACTGACTCACTCATCGTGTTGTTCCGCTTCGTCTACCCAGCTTTTCAACGTGACAGAGTCAGGTTTGCCGCTGCCAAGTAATGGCATCTGTTTCAGATAGCGAATATCGCGCGGTACAGCAAGCTCCgg                                            >  2:207313/1‑140 (MQ=255)                                                                   tgtTAGTGTGCACTGACTCACTCATCGTGTTGTTCCGCTTCGTCTACCCAGCTTTTCAACGTGACAGAGTCAGGTTTGCCGCTGCCAAGTAATGGCATCTGTTTCAGATAGCGAATATCGCGCGGTACAGCAAGCTCCgg                                            <  1:207313/140‑1 (MQ=255)                                                                           tgCACTGACTCACTCATCGTGTTGTTCCGCTTCGTCTACCCAGCTTTTCAACGTGACAGAGTCAGGTTTGCCGCTGCCAAGTAATGGCATCTGTTTCAGATAGCGAATATCGCGCGGTACAGCAAGCTCCGGCACGCCGt                                    >  2:748755/1‑140 (MQ=255)                                                                             cACTGACTCACTCATCGTGTTGTTCCGCTTCGTCTACCCAGCTTTTCAACGTGACAGAGTCAGGTTTGCCGCTGCCAAGTAATGGCATCTGTTTCAGATAGCTAATATCGAGCGATAAAGCAAGCTCCGGCACGCCGTGc                                  >  1:276927/1‑140 (MQ=255)                                                                             cACTGACTCACTCATCGTGTTGTTCCGCTTCGTCTACCCAGCTTTTCAACGTGACAGAGTCAGGTTTGCCGCTGCCAAGTAATGGCATCTGTTTCAGATAGCGAATATCGCGCGGTACAGCAAGCTCCGGCACGCCGTGc                                  >  1:627342/1‑140 (MQ=255)                                                                             cACTGACTCACTCATCGTGTTGTTCCGCTTCGTCTACCCAGCTTTTCAACGTGACAGAGTCAGGTTTGCCGCTGCCAAGTAATGGCATCTGTTTCAGATAGCGAATATCGCGCGGTACAGCAAGCTCCGGCACGCCGTGc                                  >  1:165537/1‑140 (MQ=255)                                                                             cACTGACTCACTCATCGTGTTGTTCCGCTTCGTCTACCCAGCTTTTCAACGTGACAGAGTCAGGTTTGCCGCTGCCAAGTAATGGCATCTGTTTCAGATAGCGAATATCGCGCGGTACAGCAAGCTCCGGCACGCCGTGc                                  >  2:753789/1‑140 (MQ=255)                                                                                      actcaTCGTGTTGTTCCGCTTCGTCTACCCAGCTTTTCAACGTGACAGAGTCAGGTTTGCCGCTGCCAAGTAATGGCATCTGTTTCAGATAGCGAATATCGCGCGGTACAGCAAGCTCCGGCACGCCGTGCTCGCGGGCa                         <  1:833651/140‑1 (MQ=255)                                                                                      actcaTCGTGTTGTTCCGCTTCGTCTACCCAGCTTTTCAACGTGACAGAGTCAGGTTTGCCGCTGCCAAGTAATGGCATCTGTTTCAGATAGCGAATATCGCGCGGTACAGCAAGCTCCGGCACGCCGTGCTCGCGGGCa                         <  1:155638/140‑1 (MQ=255)                                                                                      actcaTCGTGTTGTTCCGCTTCGTCTACCCAGCTTTTCAACGTGACAGAGTCAGGTTTGCCGCTGCCAAGTAATGGCATCTGTTTCAGATAGCGAATATCGCGCGGTACAGCAAGCTCCGGCACGCCGTGCTCGCGGGCa                         <  1:781337/140‑1 (MQ=255)                                                                                       ctcaTCGTGTTGTTCCGCTTCGTCTACCCAGCTTTTCAACGTGACAGAGTCAGGTTTGCCGCTGCCAAGTAATGGCATCTGTTTCAGATAGCGAATATCGCGGGGTACAGGAAGTTCTGGCACGCCGTGCTCGCGGGCAt                        >  1:359113/1‑140 (MQ=255)                                                                                                  gttCCGCTTCGTCTACCCAGCTTTTCAACGGGACAGAGTCAGGTTTGCCGCTGCCAAGTAATGGCATCTGTTTCAGATAGCGAATATCGCGCGGTACCGCAAGCTCCGGCACGCCGTGCTCGCGGGCATACTGTTGCAAc             >  2:588950/1‑140 (MQ=255)                                                                                                      cGCTTCGTCTACCCAGCTTTTCAACGTGACAGAGTCAGGTTTGCCGCTGCCAAGTAATGGCATCTGTTTCAGATAGCGAATATCGCGCGGTACAGCAAGCTCCGCCACGCCGTGCTCCCGGGCATACTGTTGCAACTTAt         >  2:329522/1‑140 (MQ=255)                                                                                                             tCTACCCAGCTTTTCAACGTGATAGTGTCCGGTTTGCCGCTGCCAAGTAATGGCATCTGTTTCAGATAGCGAATATCGCGCGGTACAGCAAGCTCCGGCACGCCGTGCTCGCGGGCATACTGTTGCAACTTATCGCGCGt  <  1:311490/140‑1 (MQ=255)                                                                                                                                     |                                                                                                                    TATCGCCAAACGCAGAGAGAAACTGCGCCACGATAACCGCTTTCATCCCCTTCGACCACAACGAAGTGTTAGTGTGCACTGACTCACTCATCGTGTTGTTCCGCTTCGTCTACCCAGCTTTTCAACGTGACAAAGTCAGGTTTGCCGCTGCCAAGTAATGGCATCTGTTTCAGATAGCGAATATCGCGCGGTACAGCAAGCTCCGGCACGCCGTGCTCGCGGGCATACTGTTGCAACTTATCGCGCGT  >  NC_000913/2973772‑2974019

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑