Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,004,535	C→T	A418T (GCC→ACC)	ygeV ←	putative sigma(54)‑dependent transcriptional regulator YgeV

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,004,535	0	C	T	100.0%	219.4 / NA	65	A418T (GCC→ACC)	ygeV	putative sigma(54)‑dependent transcriptional regulator YgeV
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (27/38);  total (27/38)

GTATGCAGATGTAAAAAGTAATGCACCAATAGTTCAATATCTTCCTGACGTTCACGCAGCGGTGGCAGA‑GTTATCGGGATAACATTAAGTCGGTAGAAGAGATCTTCGCGGAATTTACCTTCGGCAATGAACTGGGCCAAATTCTGATTAGTTGCAGAAATGATGCGAATGTCGACTTGTATTGGGCTACTGGCACCAATCGGCAGAATTTCACGTGCCTCAATAGCGCGCAGTAATTTAGCCTGCAACATTAATGGCATATCACCTATTTCA  >  NC_000913/3004399‑3004671                                                                                                                                          |                                                                                                                                         ttATGCAGATGTAAAAAGTAATTCACCAATAGTTGCATATCTTACTGATGCTCACGCAGCGGTGGCAGA‑GTTATCGAGATCACATTAAGTCGGAAGAAGAGATCTTCGCGGAATTTACCTTCGGCAATGAGCTGGGTCaa                                                                                                                                       <  2:364368/139‑1 (MQ=255)    tGCAGATGTAAAAAGTAATGCACCAATAGTTCAATATCTTCCTGACGTTCACGCAGCGGTGGCAGA‑GTTATCGGGATAACATTAAGTCGGTAGAAGAGATCTTCGCGGAATTTACCTTCGGCAATGAACTGGGTCAAAtt                                                                                                                                    <  1:628449/140‑1 (MQ=255)             aaaaaGTAATGCACCAATAGTTAAATATCTTACTGACGTTCACGCAGCGGTGGCAGA‑GTTATCGGGATAACATTAAGTCGGTAGAAGAGATCTTCGCGGAATTTACCTTCGGCAATGAACTGGGTCAAATTCTGATTAGt                                                                                                                           <  2:272566/140‑1 (MQ=255)                 aGTAATGCACCAATAGTTCAATATCTTCCTGACGTTCACGCAGCGGTGGCAGA‑GTTATCGGGATAACATTAAGTCGGTAGAAGAGATCTTCGCGGAATTTACCTTCGGCAATGAACTGGGTCAAATTCTGATTAGTTGCa                                                                                                                       <  2:651715/140‑1 (MQ=255)                      tGCACCAACAGTTCAATATCTTCCTGACGTTCAAGCAGCCGTGGCAGA‑TTTATCGGGATAACATTAAGTCGGTAGAAGAGATCTTCGCGGAATTTACCTTCGGCAATGAACTGGGTCAAATTCTGATTAGTTGCAGAAat                                                                                                                  <  2:402138/140‑1 (MQ=255)                         aCCAATAGTTCAATATCTTCCTGACGTTCACGCAGCGGTGGCAGA‑GTTATCGGGATAACATTAAGTCGGTAGAAGAGATCTTCGCGGAATTTACCTTCGGCAATGAACTGGGTCAAATTCTGATTAGTTGCAGAAatgat                                                                                                               >  1:202772/1‑140 (MQ=255)                           cAATAGTTCAATATCTTCCTGACGTTCACGCAGCGGTGGCAGA‑GTTATCGGGATAACATTAAGTCGGTAGAAGAGATCTTCGCGGAATTTATCTTCGGCAGTGATCTGGGTCAAATTGTGATTAGTTGCAGAAATGATGc                                                                                                             >  2:303161/1‑140 (MQ=255)                                 ttCAATATCTTCCTGACGTTCACGCAGCGGTGGCAGA‑GTTATCGGGATAACATTAAGTCGGTAGAAGAGATCTTCGCGGAATTTACCTTCGGCATTGAACTGAGTCAAATTCTGATTAGTTGCAGAAATGATGCGAATGt                                                                                                       >  1:70616/1‑140 (MQ=255)                                 ttCAATATCTTCCTGACGTTCACGCAGCGGTGGCAGA‑GTTATCGGGATAACATTAAGTCGGTAGAAGAGATCTTCGCGGAATTTACCTTCGGCAATGAACTGGGTCAAATTCTGATTAGTTGCAGAAATGATGCGAATGt                                                                                                       >  1:57023/1‑140 (MQ=255)                                   cAATATCTTCCTGACGTTCACGCAGCGGTGGCAGA‑GTTATCGGGATAACATTAAGTCGGAAGAAGAGATCTTGGCGGAATTTACCTTCGGCGATGAACTGAGTCGAATACTGATTAGGTGCATAAATGATGCGAATGTCg                                                                                                     >  2:226507/1‑140 (MQ=255)                                     atatCTTCCTGACGTTCACGCAGCGGTGGCAGA‑GTTATCGGGATAACATTAAGTCGGTAGAAGAGATCTTCGCGGAATTTACCTTCGGCAATGAACTGGGTCAAATTCTGATTAGTTGCAGAAATGATGCGAATGTCGAc                                                                                                   <  1:790446/140‑1 (MQ=255)                                     atatCTTCCTGACGTGCACGCACCGGTGGCAGA‑GTTACCGGGATAACATTAAGTCGGTAGAAGAGATCTTCGCGGAATTTACCTTCGGCAATGAACTGGGTCAAATTCTGATTAGTTGCAGAAATGATGCGAATGTCGAc                                                                                                   <  1:339739/140‑1 (MQ=255)                                     aaatCTTCCTGCCGTTCACGCAGCGGTGG‑AGAGGTTAT‑GGGATAACATTAAGTCGGTAGAAGAGATCTTCGCGGAATTTACCTTCGGCAATGAACTGGGTCAAATTCTGATTAGTTGCAGAAATGATGCGAATGTCGACt                                                                                                  <  1:688537/138‑1 (MQ=255)                                      tatCTTCCTGACGTTCACGCAGCGGTGGCAGA‑GTTATCGGGATAACATTAAGTCGGTAGAAGAGATCTTCGCGGAATTTACCTTCGGCAATGAACTGGGTCAAATTCTGATTAGTTGCAGAAATGATGCGAATGTCGACt                                                                                                  >  1:72809/1‑140 (MQ=255)                                        tCTTCCTGACGTTCACGCAGCGGTGGCAGA‑GTTGTCGGGATAACATTAAGTCGGTAGAAGAGATCTTCGCGGAATTTACCTTCGGCAATGAACTGGGTCAAATTCTGATTAGTTGCAGAAATGATGCGAATGTCGACTTg                                                                                                <  2:2211/140‑1 (MQ=255)                                         cTTCCTGGCGTTCACGCAGCGGTGGCAGA‑GTTATCGGGATAACATTAAGTCGGTAGAAGAGATCTTCGCGGAATTTACCTTCGGCAATGAACTGGGTCAAGTTCTGATTAGTTGCAGAAATGATGCGAATGTCGACTTGt                                                                                               >  2:379117/1‑140 (MQ=255)                                         cTTCCTGACGTTCACGCAGCGGAGGCAGA‑GTTATCGGGATAACATTGAGTCGGTAGAAGAGATCTTCGCGGAATTTACCTTCGGCAATGAACTGGGTCAAATTCTGATTAGTTGCAGAAATGATGCGAATGTCGACTTGt                                                                                               >  2:520365/1‑140 (MQ=255)                                          ttCCTGACGTTCACGCAGCGGTGGCAGA‑GTTATCGGGATAACATTAAGTCGGTAGAAGAGATCTTCGCGGAATTTACCTTCGGCAATGAACTGGGTCAAATTCTGATTAGTTGCAGAAATGATGCGAATGTCGACTTGTa                                                                                              <  1:91622/140‑1 (MQ=255)                                          ttCCTGACGTTCACGCAGCGGTGGCAGA‑GTTATCGGGATAACATTAAGTCGGTAGAAGAGATCTTCGCGGAATTTACCTTCGGCAATGAACTGGGTCAAATTCTGATTAGTTGCAGAAATGATGCGAATGTCGACTTGTa                                                                                              <  1:819909/140‑1 (MQ=255)                                           tCCTGACGTTCACGCGGCGGTGTCACA‑GTTATCGGGACAACATTAAGTCGGTAGAAGAGATCTTCGCGGAATTTACCTTCGGCAATGAACTGGGTCAAATTCTGATTAGTTGCAGAAATGATGCGAATGTCGACTTGTAt                                                                                             <  1:347516/140‑1 (MQ=255)                                           tCCTGACGTTCACGCAGCGGTGGCAGA‑GTTATCGGGATAACATTAAGTCGGTAGAAGAGATCTTCGCGGAATTTACCTTCGGCAATGAACTGGGTCAAATTCTGCTTAACTGCAGAAATGATACGAATGTCGACTTGTAt                                                                                             >  2:415162/1‑140 (MQ=255)                                           tCCTGACGTTCACGCAGCGGTGGCAGA‑GTTATCGGGATAACATTAAGTCGGTAGAAGAGATCTTCGCGGAATTTACCTTCGGCAATGAACTGGGTCAAATTCTGATTAGTTGCAGAAATGATGCGAATGTCGACTTGTAt                                                                                             >  2:543927/1‑140 (MQ=255)                                                  gTTCACGCAGCGGTGGCAGA‑GTTATCGGGATAACATTAAGTCGGTAGAAGAGATCTTCGCGGAATTTACCTTCGGCATTGAACTGAGTCAAATTCTGATTAGTTGCAGAAATGATGCGAATGTCGACTTGTATTGGGCTa                                                                                      <  2:70616/140‑1 (MQ=255)                                                        gCAGCGGTGGCAGA‑GTTATCGGGATAACATTAAGTCGGTAGAAGAGATCTTCGCGGAATTTACCTTCGGCAATGAACTGGGTCAAATTCTGATTAGTTGCAGAAATGATGCGAATGTCGACTTGTATTGGGCTACTGGCa                                                                                <  2:159735/140‑1 (MQ=255)                                                          aGCGGTGGCAGA‑GTTATCGGGATAACATTAAGTCGGTAGAAGAGATCTTCGCGGAATTTACCTTCGGCAATGAACTGGGTCAAATTCTGATAAGTTGCAGTAATAATGTGAATGTCGACTTGTATTGGGCTACTGGCAcc                                                                              >  2:375063/1‑140 (MQ=255)                                                               tGGCAGA‑GTTATCGGGATAACATTAAGTCGGTAGAAGAGATCTTCGCGGAATTTACCTTCGGCAATGAACTGGGTCAAATTCTGATTAGTTGCAGAAATGATGCGAATGTCGACTTGTATTGGGCTACTGGCACCAATCg                                                                         >  1:15903/1‑140 (MQ=255)                                                               tGGCAGA‑GTTATCGGAATAACATTACGTCTGTAGAAGGGATCTTCGCGGAATTTACCTTCGGCAATGAACTGGGTCAAATTCTGATTAGTTGCAGAAATGATGCGAATGTCGACTTGTATTGGGCTACTGGCACCAATCg                                                                         <  1:303161/140‑1 (MQ=255)                                                                ggCAGA‑GTTATCGGGATAACATTAAGTCGGTAGAAGAGATCTTCGCGGAATTTACCTTCGGCAATGAACTGGGTCAAATTCTGATTAGTTGCAGAAATGATGCGAATGTCGACTTGTATTGGGCTACTGGCACCAATCgg                                                                        <  2:521340/140‑1 (MQ=255)                                                                          aTCGGGATAACATTAAGTCGGTAGAAGAGATCTTCGCGGAATTTACCTTCGGCAATGAACTGGGTCAAATTCTGATTAGTTGCAGAAATGATGCGAATGTCGACTTGTATTGGGCTACTGGCACCAATCGGCAGAATTTc                                                               >  2:74265/1‑140 (MQ=255)                                                                          aTCGGGATAACATTAAGTCGGTAGAAGAGATCTTCGCGGAATTTACCTTCGGCAATGAACTGGGTCAAATCCTGATTAGTTGCAGAAATGATGCGAATGTCGACTTGTATTGGGCTACTGGCACCAATCGGCAGAATTTc                                                               >  2:177073/1‑140 (MQ=255)                                                                           tCGGGATAACATTAAGTCGGTAGAAGAGATCTTCGCGGAATTTACCTTCGGCAATGAACTGGGTCAAATTCTGATTAGTTGCAGAAATGATGCGAATGTCGACTTGTATTGGGCTACTGGCACCAATCGGCAGAATTTCa                                                              <  2:202772/140‑1 (MQ=255)                                                                             gggATAACATTAAGTCGGTAGACGATATCCTCGCGGATTTTACCTTCGGCAATGAACTGGGTCAAATTCTGATTAGTTGCAGAAATGATGCGAATGTCGACTTGTATTGGGCTACTGGCACCAATCGGCAGAATTTCACg                                                            <  1:375063/140‑1 (MQ=255)                                                                             gggATAACATTAAGTCGGTAGACGAGATCTTAGCGGAATGTACCTTCGCCAATGAACTGGGTCAAATTCTGATTAGTTGCAGAAATGATGCGAATGTCGACTTGTATTGGGCTACTGGCACCAATCGGCAGAATTTCACg                                                            <  2:327516/140‑1 (MQ=255)                                                                             gggATAACATTAAGTCGGTAGAAGAGATCTTCGCGGAATTTACCTTCGGCAATGAACTGGGTCAAATTCTGATTAGTTGCAGAAATGATGCGAATGTCGACTTGTATTGGGCTACTGGCACCAATCGGCAGAATTTCACg                                                            <  1:74265/140‑1 (MQ=255)                                                                             gggATAACATTAAGTCGGTAGAAGAGATCTTCGCGGAATTTACCTTCGGCAATGAACTGGGTCAAATTCTGATTAGTTGCAGAAATGATGCGAATGTCGACTTGTATTGGGCTACTGGCACCAATCGGCAGAATTTCACg                                                            <  2:156504/140‑1 (MQ=255)                                                                             gggATAACATTAAGTAGGTAGAAGAGATCTTCGCGGATTTTACCTTCGGCAATGAACTGGGTCAAATTCTGATTAGTTGCAGAAATGATGCGAATGTCGACTTGTATTGGGCTACTGGCACCAATCGGCAGAATTTCACg                                                            <  1:380450/140‑1 (MQ=255)                                                                                aTAACATTAAGTCGGTAGAAGAGATCTTCGCGGAATTTACCTTCGGCAATGAACTGGGTCAAATTCTGATTAGTTGCAGAAATGATGCGAATGTCGACTTGTGTTGGGCTCCTGACAGCAATCGGCAGAATTTCACGTGc                                                         >  1:327516/1‑140 (MQ=255)                                                                                aTAACATTAAGTCGGTAGAAGAGATCTTCGCGGAATTTACCTTCGGCAATGAACTGGGTCAAATTCTGATTAGTTGCAGAAATGATGCGAATGTCGACTTGTATTGGGCTACTGGCACCAATCGGCAGAATTTCACGTGc                                                         >  2:147930/1‑140 (MQ=255)                                                                                  aaCATTAAGTCGGTAGAAGAGATCTTCGCGGAATTTACCTTCGGCAATGAACTGGGTCAAATTCTGATTAGTTGCAGAAATGATGCGAATGTCGACTTGTATTGGGCTACTGGCACCAATCGGCAGAATTTCa                                                              >  2:808113/1‑133 (MQ=255)                                                                                  aaCATTAAGTCGGTAGAAGAGATCTTCGCGGAATTTACCTTCGGCAATGAACTGGGTCAAATTCTGATTAGTTGCAGAAATGATGCGAATGTCGACTTGTATTGGGCTACTGGCACCAATCGGCAGAATTTCa                                                              <  1:808113/133‑1 (MQ=255)                                                                                  aaCATTAAGTCGGTAGAAGAGATCTTCGCGGAATTTACCTTCGGCAATGAACTGGGTCAAATTCTGATTAGTTGCAGAAATGATGCGAATGACGACTTGTTTTGGGCTTCTGGCACCAATCGCCAGAATTTCACGTGCCt                                                       >  2:349664/1‑140 (MQ=255)                                                                                         aGTCGGTAGAAGAGATCTTCGCGGAATTTACCTTCGGCAATGAACTGGGTCAAATTCTGATTAGTTGCAGAAATGATGCGAATGTCGACTTGTATTGGGCTACTGGCACCAATCGGCAGAATTTCa                                                              <  2:520735/126‑1 (MQ=255)                                                                                         aGTCGGTAGAAGAGATCTTCGCGGAATTTACCTTCGGCAATGAACTGGGTCAAATTCTGATTAGTTGCAGAAATGATGCGAATGTCGACTTGTATTGGGCTACTGGCACCAATCGGCAGAATTTCa                                                              >  1:520735/1‑126 (MQ=255)                                                                                         aGTCGGTAGAAGAGATCTTCGCGGAATTTACCTTCGGCAATGAACTGGGTCAAATTCTGATTAGTTGCAGAAATGATGCGAATGTCGACTTATATTGGGCTACTAGCAGCAATCGGCAGAATGTCACGTGCCTCAGTAgc                                                >  2:279496/1‑140 (MQ=255)                                                                                          gTCGGTAGAAGAGATCTTCGCGCAAATTACCTTCGGCCATGAACTGGGTCAAATTCTGATTAGTTGCAGAAATGATGCGAATGTCGACTTGTATTGGGCTACTGGCACCAATCGGCAGAATTTCACGTGCCTCAATAgcg                                               <  1:224295/140‑1 (MQ=255)                                                                                            gagacagagagATCTTCCCGGAATTTACCTTTGGCTATGCACTGGGTCGAATTCTGATTAGTTGCAGAAATGATGCGAATGTCGACTTGTATTGGGCTACTGGCACCAATCGGCAGAATTTCACGTGCCTCAATAgcgcg                                             <  2:325680/133‑1 (MQ=255)                                                                                            cGGTAGAAGAGATCTTCGCGGACTTGACCTTCGGCAATGAACTGGGTCAAATTCTGATTAGTTGCAGAAATGATGCGAATGTCGACTTGTATTGGGCTACTGGCACCAATCGGCAGAATTTCACGTGCCTCAATAgcgcg                                             <  1:415162/140‑1 (MQ=255)                                                                                            cGGTAGAAGAGATCTTCGCGGAATTTACCTTCGGCAATGAACTGGGTCAAATTCTGATTAGTTGCAGAAATGATGCGAATGTCGACTTGTATTGGGCTACTGGCACCAATCGGCAGAATTTCACGTGCCTCAATAgcgcg                                             <  1:543927/140‑1 (MQ=255)                                                                                              gTAGAAGAGATCTTCGCGGAATTTACCTTCGGCAATGAACTGGGTCAAATTCTGATTAGTTGCAGAAATGATGCGAATGTCGACTTGTATTGGGCTACTGGCACCAATCGGCAGTATTTCACGTTCCTCAATAGCGCGCa                                           >  1:359117/1‑140 (MQ=255)                                                                                              gTAGAAGAGATCTTCGCGGAATTTACCTTCGGCAATGAACTGGGTCAAATTCTGATTAGTTGCAGAAATGATGCGAATGTCGACTTGTATTGGGCTACTGGCACCAATCGCCAGAATTTCACGTTCCTCAATAGCGCGCa                                           >  1:349695/1‑140 (MQ=255)                                                                                                        tCTTCGCGGAATTTAGCTTCGGAAACGAACTGGGTCATATTCTGATTAGTTGCAGAAATGATGCGAATGTCGACTTGTATTGGGCTACTGGCACCAATCGGCAGAATTTCACGTGCCTCAGTAGCGCGCAGTAATTTAGc                                 <  1:279496/140‑1 (MQ=255)                                                                                                         cTTCGCGGAATTTACCTTCGGGAATGAACTGGGTCAAATTCTGATTAGTTGCAGAAATGATGCGAATGTCGACTTGTATTGGGCTACTGGCACCAATCGGCAGAATTTCACGTGCCTCAATAGCGCGCAGTAATTTAGcc                                >  1:681419/1‑140 (MQ=255)                                                                                                         cTTCGCGGAATTTACCTTCGGCAATGAACTGGGTCAAATTCTGATTAGTTGCAGAAATGATGCGAATGTCGACTTGTATTGGGCTACTGGCACCAATCGGCAGAATTTCACGTGCCTCAATAGCGCGCAGTAATTTAGc                                 >  1:175174/1‑139 (MQ=255)                                                                                                         cTTCGCGGAATTTACCTTCGGCAATGAACTGGGTCAAATTCTGATTAGTTGCAGAAATGATGCGAATGTCGACTTGTATTGGGCTACTGGCACCAATCGGCAGAATTTCACGTGCCTCAATAGCGCGCAGTAATTTAGc                                 <  2:175174/139‑1 (MQ=255)                                                                                                             gcgGAATTTACCTTCCGCAATGCACAGGGCCAAATTCAGATTAGTTGCAGAAATGATGCGAATGTCGACTTGTATTGGGCTACTGGCACCAATCGGCAGAATTTCACGTGCCTCAATAGCGCGCAGTAATTTAGCCTGCa                            <  1:226507/140‑1 (MQ=255)                                                                                                              cgGAATTTACCTTCGGCAATGAACTGGGTCAAATTCTGATTAGTTGCAGAAATGATGCGAATGTCGACTTGTATTGGGCTACTGGCACCAATCGGCAGAATTGCACGTGCATCACTACCGCGCATTAATTTAGctgtctc                           >  1:325680/1‑134 (MQ=255)                                                                                                               ggAATTTACCTTCGGCAATGAACTGGGTCAAATTCTGATTAGTTGCAGAAATGATGCGAATGTCGACTTGTATTGGGCTACTGGCACCAATCGGCAGAATTTCACGTGCCTCAATAGCGCGCAGTAATTTAGCCTGCAAc                          >  1:446830/1‑140 (MQ=255)                                                                                                                    ttACCTTCGGCAATGAACTGGGTCAAATTCTGATTAGTTGCAGAAATGATGCGAATGTCGACTTGTATTGGGCTACTGGCACCAATCGGCAGAATTTCACGTGCCTCAATAGCGCGCAGTAATTTAGCCTGCAACATTaa                     <  1:804267/140‑1 (MQ=255)                                                                                                                     tACCTTCGGCAATGAACTGGGTCAAATTCTGATTAGTTGCAGAAATGATGCGAATGTCGACTTGTATTGGGCTACTGGCACCAATCGGCAGAATTTCACGTGCCTCAATAGCGCGCAGTAATTTAGCCTGCAACATTAAt                    <  2:15903/140‑1 (MQ=255)                                                                                                                        cTTCGGCAATGAACTGGCTCAACTTCTGATTAGTTCCAGCAATGATGCTAATGTCGACTTGTATTGGGCTACTGGCACCAATCGGCAGAATTTCACGTGCCTCAATAGCGCGCAGTAATTTAGCCTGCAACATTAATGGc                 <  2:228801/140‑1 (MQ=255)                                                                                                                         ttCGGCAATGAACTGGGTCAAATTCTGATTAGTTGCAGAAATGATGCGAATGTCGACTTGTATTGGGCTACTGGCACCAATCGGCAGAATTTCACGTGCCTCAATAGCGCGCAGTAATTTAGCCTGCAACATTAATGGCa                <  2:446830/140‑1 (MQ=255)                                                                                                                         ttCGGCAATGAACTGGGTCAAATTCTGATTAGTTGCAGAAATGATGCGAATGTCGACTTGTATTGGGCTACTGGCACCAATCGGCAGAATTTCACGTGCCTCAATAGCGCGCAGTAATTGAGCCTGCAACATTAATGGCa                <  2:57023/140‑1 (MQ=255)                                                                                                                          tCGGGAATGAACTGGGTCAAATTCTGATTAGTTGCAGAAATGATGCGAATGTCGACTTGTATTGGGCTACTGGCACCAATCGGCAGAATTTCACGTGCCTCAATAGCGCGCAGTAATTTAGCCTGCAACATTAATGGCat               <  2:681419/140‑1 (MQ=255)                                                                                                                          tCGGCAATGAACTGGTTCAAATTCTGATTAGTTGCAGGAATGATGCGAATGTCGACTTGTATTGGGCTACTGGCACCAATCGGCAGAATTTCACGTGCCTCAATAGCGCGCAGTAATTTAGCCTGCAACATTAATGGCat               <  1:379117/140‑1 (MQ=255)                                                                                                                           cGGCAATGAACTGGGTCAAATTCTGATTAGTTGCAGAAATGATGCGAATGTCGACTTGTATTGGGCTACTGGCACCAATCGGCAGAATTTCACGTGCCTCAATAGCGCGCAGTAATTTAGCCTGCAACATTAATGGCata              >  2:579566/1‑140 (MQ=255)                                                                                                                           cGGCAATGAACTGGGTCAAATCCTGATTAGTTGCAGAAATGATGCGAATGTCGACTTGTATTGGGCTACTGGCACCAATCGGCAGAATTTCACGTGCCTCAATAGCGCGCAGTAATTTAGCCTGCAACATTAATGGCata              <  1:177073/140‑1 (MQ=255)                                                                                                                             gCAATGAACTGGGTCAAATTCTGATTAGTTGCAGAAATGATGCGAATGTCGACTTGTATTGGGCTACTGGCACCAATCGGCAGAATTTCACGTGCCTCAATAGCGCGCAGTAATTTAGCCTGCAACATTAATGGCATATc            >  2:89412/1‑140 (MQ=255)                                                                                                                                  gAACTGGGTCAAATTCTGATTAGTTGCAGAAATGATGCGAATGTCGACTTGTATTGGGCTACTGGCACCAATCGGCAGAATTTCACGTGCCTCAATAGCGCGCAGTAATTTAGCCTGCAACATTAATGGCATATCACCTa       >  2:185402/1‑140 (MQ=255)                                                                                                                                       gggTCAAATTCTGATTATTTGCTGAAATGATTCGATTGTTGACTTGTATTGGGCTACTGGCACCAATCGGCAGAATTTCACGTGCCTCAATAGCGCGCAGTAATTTAGCCTGCAACATTAATGGCATATCACCTATTTCa  <  2:359117/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                         GTATGCAGATGTAAAAAGTAATGCACCAATAGTTCAATATCTTCCTGACGTTCACGCAGCGGTGGCAGA‑GTTATCGGGATAACATTAAGTCGGTAGAAGAGATCTTCGCGGAATTTACCTTCGGCAATGAACTGGGCCAAATTCTGATTAGTTGCAGAAATGATGCGAATGTCGACTTGTATTGGGCTACTGGCACCAATCGGCAGAATTTCACGTGCCTCAATAGCGCGCAGTAATTTAGCCTGCAACATTAATGGCATATCACCTATTTCA  >  NC_000913/3004399‑3004671

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑