Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,043,737	G→A	G26S (GGC→AGC)	bglA →	6‑phospho‑beta‑glucosidase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,043,737	0	G	A	100.0%	190.3 / NA	55	G26S (GGC→AGC)	bglA	6‑phospho‑beta‑glucosidase A
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (31/24);  total (31/24)

ATGTCGTTTGGCTGCATGGTACTATTTCCTGTAAGAATTGACTCATCTGGAGCCTATGATAGTGAAAAAACTCACCTTACCGAAAGATTTCTTATGGGGCGGCGCAGTTGCCGCTCATCAGGTCGAAGGCGGCTGGAACAAAGGCGGAAAAGGGCCGAGCATTTGTGACGTTCTGACCGGTGGCGCACACGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGGAAAATACTATCCAAACCATGAAGCCGTTGATTTTT  >  NC_000913/3043607‑3043869                                                                                                                                   |                                                                                                                                     aTGTCGTTTGGCTGCATGGTACTATTTCCTGTAAGAATTGACTCATCTGGAGCCTATGATAGTGAAAAAACTCACCTTACCGAAAGATTTCTTATGGGGCGGCGCAGTTGCCGCTCATCAGGTCGCGAGCAGCTGGGACa                                                                                                                             >  2:803157/1‑140 (MQ=255) aTGTCGTTTGGCTGCATGGTACTATTTCCTGTAAGAATTGACTCATCTGGAGCCTATGATAGTGAAAAAACTCACCTTACCGAAAGATTTCTTATGGGGCGGCGCAGTTGCCGCTCATCAGGTCGAAGGCAGCTGGAACa                                                                                                                             <  1:500461/140‑1 (MQ=255) aTGTCGTTTGGCTGCATGGTACTATTTCCTGTAAGAATTGACTCATCTGGAGCCTATGATAGTGAAAAAACTCACCTTACCGAAAGATTTCTTATGGGGCGGAGCAGTTGCCGCACAGCAGGTCTAAGGCAGCTGGAACa                                                                                                                             >  1:278195/1‑140 (MQ=255)       tttGGCTGCATGGTACTATTTCCTGTAAGAATTGACTCATCTGGAGCCTATGATAGTGAAAAAACTCACCTTACCGAAAGATTTCTTATGGGGCGGCGCAGTTGCCGCTCATCAGGTCGAAGGCAGCTGGAACAAAGGCg                                                                                                                       >  1:573650/1‑140 (MQ=255)           gttGCATGGTACTATTTCCTGTAAGAATTGACTCATCTGGAGCCTATGATAGTGAAAAAACTCACCTTACCGAAAGATTTCTTATGGGGCGGCGCAGTTGCCGCTCATCAGGTCGAAGGCAGCTGGAACAAAGGCGGaaa                                                                                                                   >  1:832809/3‑140 (MQ=255)                   gTACTATTTCCTGTAAGAATTGACTCATCTGGAGCCTATGATAGTGAAAAAACTCACCTTACCGAAAGATTTCTTATGGGGCGGCGCAGTTGCCGCTCATCAGGTCGAAGGCAGCTGGAACAAAGGCGGAAAAGGGCCGa                                                                                                           <  2:536978/140‑1 (MQ=255)                    tACTATTTCCTGTAAGAATTGAATCATCTGGAGCCAATGTTAGTGAAAAAACTCACCTTACCGAAAGATTTCTTATGGGGCGGCGCAGTTGCCGCTCATCAGGTCGAAGGCAGCTGGAACAAAGGCGGAAAAGGGCCGAg                                                                                                          <  2:278195/140‑1 (MQ=255)                         tttCCTGTAAGAATTGACTCATCTGGAGCCTATGATAGTGAAAAAACTCACCTTACCGAAAGATTTCTTATGGGGCGGCGCAGTTGCCGCTCATCAGGTCGAAGGCAGCTGGAACAAAGGCGGAAAAGGGCCGAGCAttt                                                                                                     >  1:589127/1‑140 (MQ=255)                             cTGTAAGAATTGACTCATCTGGAGCCTATGATAGTGAAAAAACTCACCTTACCGAAAGATTTCTTATGGGGCGGCGCAGTTGCCGCTCATCAGGTCGAAGGCAGCTGGAACAAAGGCGGAAAAGGGCCGAGCATTTGTGa                                                                                                 >  1:861203/1‑140 (MQ=255)                              tGTAAGAATTGACTTATCTGGAGCCTATGATAGTGAAAAAACTCACCTTACCGAAAGATTTCTTATGGGGCGGCGCAGTTGCCGCTCATCAGGTCGAAGGCAGCTGGAACAAAGGCGGAAAAGGGCCGAGCATTTGTGAc                                                                                                >  2:630556/1‑140 (MQ=255)                              tGTAAGAATTGACTCATCTGGAGCCTATGATAGTGAAAAAACTCACCTTACCGAAAGATTTCTTATGGGGCGGCGCAGTTGCCGCTCATCAGGTCGAAGGCATCTGGAACAAAGACGGAAAAGGTCCGAGCATTTGTGAc                                                                                                >  1:316230/1‑140 (MQ=255)                              tGTAAGAATTGACTCATCTGGAGCCTATGATAGTGAAAAAACTCACCTTACCGAAAGATTTCTTATGGGGCGGCGCAGTTGCCGCTCATCAGGTCGAAGGCAGCTGGAACAAAGGCGGAAAAGGGCCGAGCATTtgtg                                                                                                  >  1:619755/1‑138 (MQ=255)                              tGTAAGAATTGACTCATCTGGAGCCTATGATAGTGAAAAAACTCACCTTACCGAAAGATTTCTTATGGGGCGGCGCAGTTGCCGCTCATCAGGTCGAAGGCAGCTGGAACAAAGGCGGAAAAGGGCCGAGCATTtgtg                                                                                                  <  2:619755/138‑1 (MQ=255)                              tGTAAGAATTGACTCATCTGGAGCCTATGATAGTGAAAAAACTCACCTTACCGAAAGATTTCTTATGGGGCGGCGCAGTTGCCGCTCATCAGGTCGAAGGCAGCTGGAACAAAGGCGGAAAAGGGCCGAGCATTTGTGAc                                                                                                >  1:171658/1‑140 (MQ=255)                              tGTAAGAATTGACTCATCTGGAGCCTATGATAGTGAAAAAACTCACCTTACCGAAAGATTTCTTATGGGGCGGCGCAGTTGCCGCTCATCAGGTCGAAGGCAGCTGGAACAAAGGCGGAAAAGGGCCGAGCATTTGTGAc                                                                                                >  2:722166/1‑140 (MQ=255)                              tGTAAGAATTGACTCATCTGGAGCCTATGATAGTGAAAAAACTCACCTTACCGAAAGATTTCTTATGGGGCGGCGCAGTTGCCGCTCATCAGGTCGAAGGCAGCTGGAACAAAGGCGGAAAAGGGCCGAGCATTTGTGAc                                                                                                >  2:751263/1‑140 (MQ=255)                              tGTAAGAATTGACTCATCTGGAGCCTATGATAGTGAAAAAACTCACCTTACCGAAAGATTTCTTATGGGGCGGCGCAGTTGCCGCTCATCAAGTCGAAGGCAGCGGGATCAAAGGCGGAAAAGGGCCGAGCATTTGTGAc                                                                                                >  2:218676/1‑140 (MQ=255)                               gTAAGAATTGACTTATCTGGAGCCTATGATAGTGAAAAAACTCACCTTACCGAAAGATTTCTTATGGGGCGGCGCAGTTGCCGCTCATCAGGTCGAAGGCAGCTGGAACAAAGGCGGAAAAGGGCCGAGCATTTGTGACg                                                                                               <  1:630556/140‑1 (MQ=255)                                          cTCATCTGGAGCCTATGATAGTGAAAAAACTCACCTTACCGAAAGATTTCTTATGGGGCGGCGCAGTTGCCGCTCATCAGGTCGAAGGCAGCTGGAACAAAGGCGGAAAAGGGCCGAGCATTTGTGACGTTCTGACCGGt                                                                                    >  1:513685/1‑140 (MQ=255)                                          cTCATCTGGAGCCTATGATAGTGAAAAAACTCACCTTACCGAAAGATTTCTTATGGGGCGGCGCAGTTGCCGCTCATCAGGTCGAAGGCAGCTGGAACAAAGGCGGAAAAGGGCCGAGCATTTGTGACGTTCTGACCGGt                                                                                    >  1:745620/1‑140 (MQ=255)                                           tCATCTGGAGCCTATGATAGTGAAAAAACTCACCTTACCGAAAGATTTCTTATGGGGCGGCGCAGTTGCCGCTCATCAGGTCGAAGGCAGCTGGAACAAAGGCGGAAAAGGGCCGAGCATTTGTGACGTTCTGACCGGTg                                                                                   >  1:810775/1‑140 (MQ=255)                                                 ggAGCCTATGATAGTGAAAAAACTCACCTTACCGAAAGATTTCTTATGGGGCGGCGCAGTTGCCGCTCATCAGGTCGAAGGCAGCTGGAACAAAGGCGGAAAAGGGCCGAGCATTTGTGACGTTCTGACCGGTGGCGcac                                                                             <  1:803157/140‑1 (MQ=255)                                                       tATGATAGTGAAAAAACTCACCTTACCGAAAGATTTCTTATGGGGCGGCGCAGTTGCCGCTCATCAGGTCGAAGGCAGCTGGAACAAAGGCGGAAAAGGGCCGAGCATTTGTGACGTTCTGACCGGTGGCGCACACGGCg                                                                       >  2:35753/1‑140 (MQ=255)                                                       tATGATAGTGAAAAAACTCACCTTACCGAAAGATTTCTTATGGGGCGGCGCAGTTGCCGCTCATCAGGTCGAAGGCAGCTGGAACAAAGGCGGAAAAGGGCCGAGCATTTGTGAAGTGCTGACCGGTGGCGCACACGGCg                                                                       >  1:343388/1‑140 (MQ=255)                                                         tGATAGTGAAAAAACTCACCTTACCGAAAGATTTCTTATGGGGCGGCGCAGTTGCCGCTCATCAGGTCGAAGGCAGCTGGAACAAAGGCGGAAAAGGGCCGAGCATTTGTGACGTTCTGACCGGTGGCGCACACGGCGTg                                                                     >  2:9366/1‑140 (MQ=255)                                                               tGAAAAAACTCACCTTACCGAAAGATTTCTTATGGGGCGGCGCAGTTGCCGCTCTTCAGGTCGAAGGCAGCTGGAACAAAGGCGGAAAAGGCCCGAGCATTTGTGACGATCTGACCGGTGGCCCACACGGCGTGCcgcgc                                                               >  2:367005/1‑140 (MQ=255)                                                               tGAAAAAACTCACCTTACCGAAAGATTTCTTATGGGGCGGCGCAGTTGCCGCTCATCAGGTCGAAGGCAGCTGGAACAAAGGCGGAAAAGGTCCGAGCATGTGTTACGGTCTGACCGATGGCACACACGGCGTGCcgcgc                                                               >  2:237286/1‑140 (MQ=255)                                                               tGAAAAAACTCACCTTACCGAAAGATTTCTTATGGGGCGGCGCAGTTGCCGCTCATCAGGTCGAAGGCAGCTGGAACAAAGGCGGAAAAGGGCCGAGCATTTGTGACGTTCTGACCGGTGGCGCACACGGCGTGCcgcgc                                                               >  1:548503/1‑140 (MQ=255)                                                               tGAAAAAACTCACCTTACCGAAAGATTTCTTATGGGGCGGCGCAGTTGCCGCTCATCAGGTCGAAGGCAGCTGGAACAAAGGCGGAAAAGGGCCGAGCATCTGTTACGTTCTGACCGCTGGCCCACACGGCGTGCcgcgc                                                               >  2:353634/1‑140 (MQ=255)                                                                      aCTCACCTTACCGAAGGATTTCTTATGGGGCGGCGCACTTGCCGCTCATCAGGTCGAAGGCAGCTGGAACAAAGGCGGAAAAGGGCCGAGCATTTGTGACGTTCTGACCGGTGGCGCACACGGCGTGCCGCGCGAAATCa                                                        <  1:353634/140‑1 (MQ=255)                                                                          aCCTTACCGAAAGATTTCTTATGGGGCGGCGCAGTTGCCGCTCATCAGGTCGAAGGCAGCTGGAACAAAGGCGGAAAAGGGCCGAGCATTTGTGACGTTCTGACCGGTGGCGCACACGGCGTGCCGCGCGaa                                                            >  2:820940/1‑132 (MQ=255)                                                                          aCCTTACCGAAAGATTTCTTATGGGGCGGCGCAGTTGCCGCTCATCAGGTCGAAGGCAGCTGGAACAAAGGCGGAAAAGGGCCGAGCATTTGTGACGTTCTGACCGGTGGCGCACACGGCGTGCCGCGCGaa                                                            <  1:820940/132‑1 (MQ=255)                                                                           ccTTACCGAAAGATTTCTTATGTGGCGGCGCGGTTCCCGTTCATCAGGCCGAAGGCAGCTGGAACAAAGGCGGAAAAGGGCCGAGCATTTGTGACGTTCTGACCGGTGGCGCACACGGCGTGCCGCGCGAAATCACCaaa                                                   <  2:343388/140‑1 (MQ=255)                                                                            cTTACCGAAAGATTTCTTATGGGGCGGCGCAGTTGCCGCTCATCAGGTCGAAGGCAGCTGGAACAAAGGCGGAAAAGGGCCGAGCATTTGTGACGTTCTGACCGGTGGCGCACACGGCGTGCCGCGCGAAATCACCAaag                                                  <  2:832809/140‑1 (MQ=255)                                                                                ccGAAAGATTTCTTATGGGGCGGCGCAGTTGCCGCTCATCAGGTCGAAGGCAGCTGGAACAAAGGCGGAAAAGGGCCGAGCATTTGTGACGTTCTGACCGGTGGCGCACACGGCGTGCCGCGCGAAATCACCAAAGAAGt                                              <  2:573650/140‑1 (MQ=255)                                                                                     aGATTTCTTATGGGGTGGCGCAATTGCCGCTCATCAGCTCGAAGGCAGCTGGAACAAAGGCGGAAAAGGGCCGAGCATTTGTGACGTTCTGACCGGTGGCGCACACGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGc                                         <  1:218676/140‑1 (MQ=255)                                                                                     aGATTTCTTATGGGGCGGCGCAGTTGCCGCTCATCAGGTCGAAGGCAGCTGGAACAAAGGCGGAAAAGGGCCGAGCATTTGTGACGTTCTGACCGGTGGCGCACACGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGc                                         <  2:171658/140‑1 (MQ=255)                                                                                     aGATTTCTTATGGGGCGGCGCAGTTGCCGCTCATCAGGTCGAAGGCAGCTGGAACAAAGGCGGAAAAGGGCCGAGCATTTGTGACGTTCTGACCGGTGGCGCACACGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGc                                         <  1:465621/140‑1 (MQ=255)                                                                                          tCTTATGGGGCGGCGCAGTTGCCGCTCATCAGGTCGAAGGCAGCTGGAACAAAGGCGGAAAAGGGCCGAGCATTTGTGACGTTCTGACCGGTGGCGCACACGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGGa                                    <  1:192322/140‑1 (MQ=255)                                                                                               tGGGGCGGCGCAGTTGCCGCTCATCAGGTCGAAGGCAGCTGGAACAAAGGCGGAAAAGGGCCGAGCATTTGTGACGTTCTGACCGGTGGCGCACACGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGGAAAATa                               <  2:548503/140‑1 (MQ=255)                                                                                                   gcggcgCAGTTGCCGCTCATCAGGTCGAAGGCAGCTGGAACAAAGGCGGAAAAGGGCCGAGCATTTGTGACGTTCTGACCGGTGGCGCACACGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGGAAAATACTAt                           <  2:589127/140‑1 (MQ=255)                                                                                                   gcggcgCAGTTGCCGCTCATCAGGTCGAAGGCAGCTGGAACAAAGGCGGAAAAGGGCCGAGCATTTGTGACGTTCTGACCGGTGGCGCACACGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGGAAAATACTAt                           <  1:9366/140‑1 (MQ=255)                                                                                                      gcgcAGTTGCCGCTCATCAGGTCGAAGGCAGCTGGAACAAAGGCGGAAAAGGGCCGAGCATTTGTGACGTTCTGACCGGTGGCGCACACGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGGAAAATACTATCCa                        >  2:676243/1‑140 (MQ=255)                                                                                                      gcgcAGTTGCCGCTCATCAGGTCGAAGGCAGCTGGAACAAAGGCGGAAAAGGGCCGAGCATTTGTGACGTTCTGACCGGTGGCGCACACGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGGAAAATACTATCCa                        >  1:781487/1‑140 (MQ=255)                                                                                                            ttGCCGCTCATCAGGTCGAAGGCAGCTGGAACAAAGGCGGAAAAGGGCCGAGCATTTGTGACGTTCTGACCGGTGGCGCACACGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGGAAAATACTATCCAAACCAt                  >  1:37328/1‑140 (MQ=255)                                                                                                            ttGCCGCTCATCAGGTCGAAGGCAGCTGGAACAAAGGCGGAAAAGGGCCGAGCATTTGTGACGTTCTGACCGGTGGCGCACACGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGGAAAATACTATCCAAACCAt                  >  1:30254/1‑140 (MQ=255)                                                                                                              gCCGCTCATCAGGTCGAAGGCAGCTGGAACAAAGGCGGAAAAGGGCCGAGCATTTGTGACGTTCTGACCGGTGGCGCACACGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGGAAAATACTATCCAAACCATGa                <  2:37328/140‑1 (MQ=255)                                                                                                                   tcatcaGGTCGAAGGCAGCTGGAACAAAGGCGGAAAAGGGCCGAGCATTTGTGACGTTCTGACCGGTGGCGCACACGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGGAAAATACTATCCAAACCATGAAGCCg           <  2:781487/140‑1 (MQ=255)                                                                                                                    catcaGGTCGAAGGCAGCTGGAACAAAGGCGGAAAAGGGCCGAGCATTTGTGACGTTCTGACCGGTGGCGCACACGGCGTGCCGCGCGAAAGCACCAAAGCAGTATTGACAGGAAAAGACTATCCAAACCATGAAGCCGt          >  2:305682/1‑140 (MQ=255)                                                                                                                     atcaGGTCGAAGGCAGCTGGAACAAAGGCGGAAAAGGGCCGAGCATTTGTGACGTTCTGACCGGTGGCGCACACGGCGTGCCGCGCGTAATCACCAAAGAAGTCTTGCCAGGAAAATACTATCCAAACCATGAAGCCGtt         <  2:513685/140‑1 (MQ=255)                                                                                                                     atcaGGTCGAAGGCAGCTGGAACAAAGGCGGAAAAGGGCCGAGCATTTGTGACGTTCTGACCGGTGGCGCACACGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGGAAAATACTATCCAAACCATGAAGCCGtt         <  2:745620/140‑1 (MQ=255)                                                                                                                      tcaGGTCGAAGGCAGCTGGAACAAAGGCGGAAAAGGGCCGAGCATTTGTGACGTTCTGACCGGTGGCGCACACGGCGTGCCGCGCGAAATCACCAAAGAAGTTTTGCCAGGAAATTACTATCCAAACCATGAAGCCGTTg        >  1:291797/1‑140 (MQ=255)                                                                                                                       caGGTCGAAGGCAGCTGGAACAAAGGCGGAAAAGGGCCGAGCATTTGTGACGTTCTGACCGGTGGCGCACACGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGGAAAATACTATCCAAACCATGAAGCCGTTGa       >  2:843786/1‑140 (MQ=255)                                                                                                                            cGAAGGCAGCTGGAACAGAGGCTGAAAAGGGTCGAGCATTTGTGACGTACTGACCGGTGGCGCACACGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGGAAAATACTATCCAAACCATGAAGCCGTTGAttttt  <  2:316230/140‑1 (MQ=255)                                                                                                                            cGAAGGCAGCTGGAACAAAGGCGGAAAAGGGCCGAGCATTTGTGACGTTCTGACCGGTGGCGCACACGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGGAAAATACTATCCAAACCATGAAGCCGTTGa       <  2:758027/135‑1 (MQ=255)                                                                                                                            cGAAGGCAGCTGGAACAAAGGCGGAAAAGGGCCGAGCATTTGTGACGTTCTGACCGGTGGCGCACACGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGGAAAATACTATCCAAACCATGAAGCCGTTGa       >  1:758027/1‑135 (MQ=255)                                                                                                                                   |                                                                                                                                     ATGTCGTTTGGCTGCATGGTACTATTTCCTGTAAGAATTGACTCATCTGGAGCCTATGATAGTGAAAAAACTCACCTTACCGAAAGATTTCTTATGGGGCGGCGCAGTTGCCGCTCATCAGGTCGAAGGCGGCTGGAACAAAGGCGGAAAAGGGCCGAGCATTTGTGACGTTCTGACCGGTGGCGCACACGGCGTGCCGCGCGAAATCACCAAAGAAGTCTTGCCAGGAAAATACTATCCAAACCATGAAGCCGTTGATTTTT  >  NC_000913/3043607‑3043869

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑