Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,045,063	T→C	Y468H (TAC→CAC)	bglA →	6‑phospho‑beta‑glucosidase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,045,063	0	T	C	100.0%	180.6 / NA	52	Y468H (TAC→CAC)	bglA	6‑phospho‑beta‑glucosidase A
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (29/23);  total (29/23)

CACACCGTGGGGCTGCATCGACTGCGTGTCGTTCACCACCGGGCAGTACAGCAAACGCTACGGCTTTATCTATGTGAATAAACATGACGACGGTACTGGCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTA  >  NC_000913/3044927‑3045199                                                                                                                                         |                                                                                                                                         cacacCGTGGGGCTGCATCGACTGCGTGTCGTTCACCACCGGGCAGTACAGCAAACGCTACGGCTTTATCTATGTGAATAAACATGACGACTGTACTGGCGATAAGGCACGTTCACGTAAGAAGAGCTTTAACTGGcaca                                                                                                                                       >  2:390948/1‑140 (MQ=255)   cacCGTGGGGCTGCATCGACTGCGTGTCGTTCACCACCGGGCAGTACAGCAAACGCTACGGCTTTATCTATGTGAATAAACATGACGACGGTACTGGCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGCACaaa                                                                                                                                     <  1:578416/140‑1 (MQ=255)   cacCGTGGGGCTGCATCGACTGCGTGTCGTTCACCACCGGGCAGTACAGCAAACGCTACGGCTTTATCTATGTGAATAAACATGACGACGGTACTGGCGATAAGTCGCGTGCACCTAAGAAGAGCTTTAACTGGCACaaa                                                                                                                                     >  1:280561/1‑140 (MQ=255)    acCGTGGGGCTGCATCGACTGCGTGTCGTTCACCACCGGGCAGTACAGCAAACGCTACGGCTTTATCTATGTGAATAAACATGACGACGGTACTGGCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGCACAAag                                                                                                                                    <  2:195934/140‑1 (MQ=255)      cGTGGGGCTGCATCGACTGCGTGTCGTTCACCACCGGGCAGTACAGCAAACGCTACGGCTTTATCTATGTGAATAAACATGACGACGGTACTGGCGATATGTAGCGTTCACGTAGGAAGAGCTTGAACTGGCACAAagag                                                                                                                                  >  1:345618/1‑140 (MQ=255)                cATCGACTGCGTGTCGTTCACCACCGGGCAGTACAGCAAACGCTACGGCTTTATCTATGTGAATAAACATGACGACGGTACTGGCGATATGTCGCGTTCAGGTATGAAAAGCTTTAACTGGCACAAAGAGGTGATTGCCa                                                                                                                        >  2:328443/1‑140 (MQ=255)                cATCGACTGCGTGTCGTTCACCACCGGGCAGTACAGCAAACGCTACGGCTTTATCTATGTGAATAAACATGACGACGGTACTGGCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGCACAAAGAGGTGATTGCCa                                                                                                                        >  2:97737/1‑140 (MQ=255)                cATCGACTGCGTGTCGTTCACCACCGGGCAGTACAGCAAACGCTACGGCTTTATCTATGTGAATAAACATGACGACGGTACTGGCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGCACAAAGAGGTGATTGCCa                                                                                                                        >  2:126018/1‑140 (MQ=255)                     aCTGCGTGTCGTTCACCACCGGGCAGTACAGCAAACGCTACGGCTTTATCTATGTGAATAAACATGACGACGGTACTGGCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGCACAAAGAGGTGATTGCCAGCAAc                                                                                                                   >  1:118252/1‑140 (MQ=255)                          gtgtCGTTCACCACCGGGCAGTACAGCAAACGCTACGGCTTTATCTATGTGAATAAACATGACGACGGTACTGGCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGCACAAAGAGGTGATTGCCAGCAACGGCga                                                                                                              >  1:30830/1‑140 (MQ=255)                           tgtCGTTCACCACCGTGCAGTACAGCAAACGCTACGGTTTTATCTATGTGAATAAACATGACGACGGTACTGGCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGCACAAAGAGGTGATTGCCAGCAACGGCgag                                                                                                             <  1:314254/140‑1 (MQ=255)                           tgtCGTTCACCACCGGGGAGTAAAGCAAACGCTACCGCTCTATCTATGCGAATAAACATGACGACGGTACTGGCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGCACAAAGAGGTGATTGCCAGCAACGGCgag                                                                                                             <  2:275089/140‑1 (MQ=255)                                ttCACCACCGGGCAGTACAGCAAACGCTACGGCTTTATCTATGTGAATAAACATGACGACGGTACTGGCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGCACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCt                                                                                                        >  1:558264/1‑140 (MQ=255)                                      cccGGGCAGTACAGCAAACGCTTCGGCTTTATCTACGTGCATAAACATCACGACGGTACTGGCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGCACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGt                                                                                                  <  2:248406/139‑1 (MQ=255)                                      accGGGCAGTACAGCAAACGCTGCGGCTTTAACTACGTGAATAAACATAACGACGGTACTGGCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGCACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGt                                                                                                  <  2:303574/140‑1 (MQ=255)                                       ccGGGCAGTACAGCAAACGCTACGGCTTTATCTATGTGAATAAACATGACGACGGTACTGGCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGCACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTc                                                                                                 <  1:126018/140‑1 (MQ=255)                                          ggCAGTACAGCAAACGCTACGGCTTTATCTATGTGAATAAACATGACGACGGTACTGGCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGCACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGAt                                                                                              >  2:293275/1‑140 (MQ=255)                                          ggCAGTACAGCAAACGCTACGGCTTTATCTATGTGAATAAACATGACGACGGTACTGGCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGCACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGAt                                                                                              >  1:27053/1‑140 (MQ=255)                                               tACAGCAAACGCTACGGCTTTATCTATGTGAATAAACATGACGACGGTACTGGCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGCACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGt                                                                                         >  2:570198/1‑140 (MQ=255)                                                  aGCAAACGCTACGGCTTTATCTATGTGAATAAACATGACGACGGTACTGGCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGCACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTAcc                                                                                      <  1:293275/140‑1 (MQ=255)                                                  aGCAAACGCTACGGCTTTATCTATGTGAATAAACATGACGACGGTACTGGCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGCACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTAcc                                                                                      <  2:701778/140‑1 (MQ=255)                                                  aGCAAACGCTACGGCTTTATCTATGTGAATAAACATGACGACGGTACTGGCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGCACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTAcc                                                                                      <  1:841916/140‑1 (MQ=255)                                                         gCTACGGCTTTATCTATGTGAATAAACATGACGACGGTACTGGCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGCACAAAGAGGTTATTGCCAGTAACCGCGGGAAGCTTTAAGTCCATGAAGTACCGGATGCa                                                                               >  2:358756/1‑140 (MQ=255)                                                         gCTACGGCTTTATCTATGTGAATAAACATGACGACGGTACTGGCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGCACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCa                                                                               >  1:152222/1‑140 (MQ=255)                                                                   tATCTATGTGAATAAACATGACGACGGTACTGGCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGCACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACttgttg                                                                     >  2:438926/1‑140 (MQ=255)                                                                     tCTATGTGAATAAACATGACGACGGTACTGGCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGCACAAAGAGGTGATTGCCAGCAACGGCGAGAAACTTTAAGTCGATGAAGAACCGAATGCAATACTTGTTGCa                                                                   >  2:265965/1‑140 (MQ=255)                                                                      cTATGTGAATAAACATGGCGACGGTACTGGCCATAAGTCTCGTTCACGTAAGAAGAGCTTTAACTGGCACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCAt                                                                  <  2:345618/140‑1 (MQ=255)                                                                         tgtgAATAAACATGACGACGGTACTGGCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGCACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTATCGGATGCAATACTTGTTGCATCCg                                                               >  1:652208/1‑140 (MQ=255)                                                                          gtgAATAAACATGACGACGGTACTGGCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGCACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCgg                                                              >  1:696763/1‑140 (MQ=255)                                                                             aaTAAACATGACGACGGTACTGTCGGTATTTCGCGTTAACGTAAGAAGAGCTTTAACTGGCACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCa                                                           <  1:262349/140‑1 (MQ=255)                                                                             aaTAAACATGACGACGGTACTGGCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGCACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCa                                                           <  2:152222/140‑1 (MQ=255)                                                                             aaTAAACATGACGACGGTACTGGCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGCACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCa                                                           >  1:636153/1‑140 (MQ=255)                                                                             aaTAAACATGACGACGGTACTGGCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGCACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCa                                                           <  1:570198/140‑1 (MQ=255)                                                                               tAAACATGACGACGGTACTGGCAATCTGTCGCGTTCATGTAAGAAGAGCTTTAACTGGCACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGa                                                         <  1:390948/140‑1 (MQ=255)                                                                                  aCATGACGACGGTACGGGCGATTTGTCGCGTTCACATAAGAAGAGCTTTAACTGGCACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACaa                                                      <  1:378546/140‑1 (MQ=255)                                                                                     tGACGACGGTACTGGCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGCACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACtt                                                   <  2:696763/140‑1 (MQ=255)                                                                                       acgacgGTACTGGCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGCACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTcc                                                 >  1:565826/1‑140 (MQ=255)                                                                                       acgacgGTACTGGCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGCACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTAGATGAAGTCCCGGATGAAATACTTGGTGCAACCGGTCAGACAACTTcc                                                 >  2:262349/1‑140 (MQ=255)                                                                                           cgGTACTGGCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGCACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGt                                             >  2:95754/1‑140 (MQ=255)                                                                                                   gCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGCACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACa                                                       <  1:803761/122‑1 (MQ=255)                                                                                                   gCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGCACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACa                                                       >  2:803761/1‑122 (MQ=255)                                                                                                      atatGTCGCGTTCACTTAAGAATAGGTTTTACTGGCACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTcccg                                  <  1:397584/140‑1 (MQ=255)                                                                                                            cgcgTTCACGTAAGACGAGCTTGAATTGGCACAAAGAAGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCcgcccgcc                            <  1:358756/140‑1 (MQ=255)                                                                                                             gcgTTCACGTAAGAAGAGCTTTAACTGGCACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCTCGCCCGCCa                           >  1:17993/1‑140 (MQ=255)                                                                                                             gcgTTCACGTAAGAAGAGCTTTAACTGGCACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCa                           >  2:42631/1‑140 (MQ=255)                                                                                                             gcgTTCACGTAAGAAGAGCTTTAACTGGCACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATAATTGTTGCACCCGGTGAAACAACTTCTCTGTCATATTTCCTGCCCGCCa                           >  2:397584/1‑140 (MQ=255)                                                                                                              cgTTCACGTAAGAAGGGCTTTAACTAGCAAAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCaa                          <  1:293712/140‑1 (MQ=255)                                                                                                                        agaagaGCTTTAACTGGCACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAGGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATaaaa                >  2:651946/1‑140 (MQ=255)                                                                                                                             ggCTTTAACTGGCACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTcc           <  2:27053/139‑1 (MQ=255)                                                                                                                               cTTTAACTGGCACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCACCAAATCGATAAAACTTCCCg         >  2:456784/1‑140 (MQ=255)                                                                                                                                tttAACTGGCACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGt        <  2:30830/140‑1 (MQ=255)                                                                                                                                 ttAACTGGCACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACGACTTCCCTATTTTATTGCCCGCCCGGCAAAGCGATAAAACTTCCCGTg       >  2:252864/1‑140 (MQ=255)                                                                                                                                    aCTGGCACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACg    >  2:741172/1‑140 (MQ=255)                                                                                                                                      tGGCACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTATCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTa  <  2:652208/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                         CACACCGTGGGGCTGCATCGACTGCGTGTCGTTCACCACCGGGCAGTACAGCAAACGCTACGGCTTTATCTATGTGAATAAACATGACGACGGTACTGGCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTA  >  NC_000913/3044927‑3045199

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑